RU2004100722A -
PES6-1 RECOMBINANT PLASMID DNA ENCODING HUMAN INTERFERON BETA-1B POLYPEPTIDE AND STRAIN ESCHERICHIA COLI BDEES6-PRODUCER OF RECOMBINANT BETA-1B INTERFERON
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PES6-1 RECOMBINANT PLASMID DNA ENCODING HUMAN INTERFERON BETA-1B POLYPEPTIDE AND STRAIN ESCHERICHIA COLI BDEES6-PRODUCER OF RECOMBINANT BETA-1B INTERFERON
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RU2004100722A
RU2004100722ARU2004100722/13ARU2004100722ARU2004100722ARU 2004100722 ARU2004100722 ARU 2004100722ARU 2004100722/13 ARU2004100722/13 ARU 2004100722/13ARU 2004100722 ARU2004100722 ARU 2004100722ARU 2004100722 ARU2004100722 ARU 2004100722A
Институт биоорганической химии им.академиков М.М. Шем кина и Ю.А. Овчинникова РАН (RU)
Институт биоорганической химии им.академиков М.М. Шемякина и Ю.А. Овчинникова РАН
Закрытое акционерное общество "Мастерклон" (RU)
Закрытое Акционерное Общество "Мастерклон"
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Application filed by Институт биоорганической химии им.академиков М.М. Шем кина и Ю.А. Овчинникова РАН (RU), Институт биоорганической химии им.академиков М.М. Шемякина и Ю.А. Овчинникова РАН, Закрытое акционерное общество "Мастерклон" (RU), Закрытое Акционерное Общество "Мастерклон"filedCriticalИнститут биоорганической химии им.академиков М.М. Шем кина и Ю.А. Овчинникова РАН (RU)
Priority to RU2004100722/13ApriorityCriticalpatent/RU2261913C1/en
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Micro-Organisms Or Cultivation Processes Thereof
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Enzymes And Modification Thereof
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Preparation Of Compounds By Using Micro-Organisms
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Claims (2)
1. Рекомбинантная плазмидная ДНК pES6-1, кодирующая полипептид с последовательностью интерферона β-1b человека, имеющая молекулярную массу 3,61 МДа (5880 п.о.); состоящая из NdeI/HindIII-фрагмента ДНК плазмиды рЕТ22b(+), содержащего промотор и терминатор транскрипции Т7-РНК-полимеразы и усилитель трансляции гена 10 фага Т7, и NdeI/HindIII-фрагмента ДНК, содержащего последовательность искусственного гена рекомбинантного интерферона β-1b человека, содержащая в качестве генетического маркера ген β-лактамазы, детерминирующей устойчивость трансформированных плазмидой pES6-1 клеток Е.coli к ампициллину, содержащая уникальные сайты узнавания эндонуклеаз рестрикции, расположенные на следующем расстоянии вправо от сайта NdeI: XbaI - 38 п.о., HpaI - 1332 п.о., PstI - 4065 п.о., PvuI - 4190 п.о., XhoI - 5363 п.о.1. Recombinant plasmid DNA pES6-1 encoding a polypeptide with a human interferon β-1b sequence, having a molecular weight of 3.61 MDa (5880 bp); consisting of the NdeI / HindIII DNA fragment of the plasmid pET22b (+) containing the promoter and terminator of transcription of the T7 RNA polymerase and the translation enhancer of the T7 phage 10 gene, and the NdeI / HindIII DNA fragment containing the sequence of the human recombinant interferon β-1b artificial gene containing, as a genetic marker, a β-lactamase gene that determines the resistance of ampicillin to E. coli cells transformed with plasmid pES6-1, containing unique recognition sites for restriction endonucleases located to the right of the site NdeI: XbaI - 38 bp, HpaI - 1332 bp, PstI - 4065 bp, PvuI - 4190 bp, XhoI - 5363 bp2. Штамм Escherichia coli BDEES6, содержащий рекомбинантную плазмидную ДНК pES6-1, - продуцент рекомбинантного интерферона β-1b человека.2. The strain Escherichia coli BDEES6 containing recombinant plasmid DNA pES6-1, the producer of recombinant human interferon β-1b.
RU2004100722/13A2004-01-152004-01-15RECOMBINANT PLASMID DNA pES6-1 ENCODING HUMAN POLYPEPTIDE INTERFERON BETA-1b AND STRAIN Escherichia coli BDEES6 AS PRODUCER OF HUMAN RECOMBINANT INTERFERON BETA-1b
RU2261913C1
(en)
RECOMBINANT PLASMID DNA pES6-1 ENCODING HUMAN POLYPEPTIDE INTERFERON BETA-1b AND STRAIN Escherichia coli BDEES6 AS PRODUCER OF HUMAN RECOMBINANT INTERFERON BETA-1b
RECOMBINANT PLASMID DNA pES6-1 ENCODING HUMAN POLYPEPTIDE INTERFERON BETA-1b AND STRAIN Escherichia coli BDEES6 AS PRODUCER OF HUMAN RECOMBINANT INTERFERON BETA-1b
RECOMBINANT PLASMID DNA pES6-1 ENCODING HUMAN POLYPEPTIDE INTERFERON BETA-1b AND STRAIN Escherichia coli BDEES6 AS PRODUCER OF HUMAN RECOMBINANT INTERFERON BETA-1b
PES6-1 RECOMBINANT PLASMID DNA ENCODING HUMAN INTERFERON BETA-1B POLYPEPTIDE AND STRAIN ESCHERICHIA COLI BDEES6-PRODUCER OF RECOMBINANT BETA-1B INTERFERON
PES4-4 RECOMBINANT PLASMID DNA ENCODING THE HUMAN INTERPHERON ALPHA-2B POLYPEPTIDE AND STRAIN ESCHERICHIA COLI BDEES4-PRODUCER OF ALF-2 RECOMBINANT INTERFERON
Recombinant plasmid DNA PES3-7 encoding a polypeptide with a sequence of granulocyte colony stimulating human factor and strain ESCHERICHIA COLI BL21 (DE3) clenco
Recombinant plasmid DNA pQe30_PS-CFP2 / TurboYFP_MBP7, coding fragment of human myelin basic protein fused 80-104 IN THE COMPOSITION OF STRUCTURES with yellow and cyan fluorescent protein EPeFRET, STRAIN Escherichia coli BL21 (DE3) - PRODUCER said protein, AND METHOD OF PRODUCING RECOMBINANT PROTEIN EPeFRET
PAS-2 RECOMBINANT PLASMID DNA ENCODING A HUMAN ASPART PROINSULINE PROLINE POLYPEPTIDE AND ESCHERICHIA COLI BAS2 STRAIN OF BACTERIA - PRODUCER OF ASPART RECOMBINANT PROINSULIN
RECOMBINANT PLASMID DNA pET-hHSP70, ENCODING THE HEAT SHOCK PROTEIN hSP70, AND THE STRAIN E. coli BL21 (DE3) / pET-hHSP70 - PRODUCT HUMAN SHOCK PROTEIN hSP70
P30NE2 RECOMBINANT PLASMID DNA ENCODING THE 181-AMINO ACID N-TERMINAL FRAGMENT OF CLASSIC PIG VIRUS VIRUS E2 GLYCOPROTEIN AND PROVIDING ITS EXCESSLESS E.
Recombinant plasmid DNA pET32a-DomIII-E-ZIKV, which provides expression of a gene fragment E encoding domain III of structural Zlyvirus glycoprotein E and recombinant protein Dom III-E-ZIKV, designed to induce specific immunity against Zika virus
RECOMBINANT PLASMID DNA PLP-3,1, ENCODING HUMAN LYSPRO PROINSULINE POLYPEPTIDE, AND STRAIN OF ESCHERICHIA COLI PLP-3-1 / TG-1 PRODUCER OF RECOMBINANT L PROINSPROIN
METHOD FOR PRODUCING RECOMBINANT URIDINE-PHOSPHORILASE, RECOMBINANT PLASMID DNA PERURPHO1 AND STRAIN ESCHERICHIA COLI BL21 (DE3) / PERURPHO1 FOR ITS IMPLEMENTATION
Recombinant Plasmid DNA PGSDEI Encoding Protein E of Tick-Encephalitis Virus and ESCHERICHIA COLI Strain - Producer of Recombinant Protein E of Tick-Encephalitis Virus
Recombinant plasmid DNA pPA-OPRF, encodes SYNTHESIS OF RECOMBINANT PROTEIN F OUTER MEMBRANE PSEUDOMONAS AERUGINOSA, STRAIN ESCHERICHIA coli PA-OPRF-RECOMBINANT PROTEIN PRODUCER F OUTER MEMBRANE PSEUDOMONAS AERUGINOSA, AND METHOD OF PRODUCING RECOMBINANT PROTEIN F OUTER MEMBRANE PSEUDOMONAS AERUGINOS
Recombinant plasmid DNA p31NC encoding 125 amino acid nucleocapsid protein of the pig reproductive and respiratory syndrome virus and ensuring its expression in E. coli bacteria cells