RU2001105774A -
Recombinant plasmid DNA p31NC encoding 125 amino acid nucleocapsid protein of the pig reproductive and respiratory syndrome virus and ensuring its expression in E. coli bacteria cells
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Recombinant plasmid DNA p31NC encoding 125 amino acid nucleocapsid protein of the pig reproductive and respiratory syndrome virus and ensuring its expression in E. coli bacteria cells
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RU2001105774A
RU2001105774ARU2001105774/13ARU2001105774ARU2001105774ARU 2001105774 ARU2001105774 ARU 2001105774ARU 2001105774/13 ARU2001105774/13 ARU 2001105774/13ARU 2001105774 ARU2001105774 ARU 2001105774ARU 2001105774 ARU2001105774 ARU 2001105774A
Федеральное государственное учреждение Всероссийский научно-исследовательский институт защиты животных
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Рекомбинантная плазмидная ДНК р31МС, кодирующая 125 аминокислотный нуклеокапсидный белок вируса репродуктивно-респираторного синдрома свиней и обеспечивающая его экспрессию в клетках бактерий E.coli, содержит BamH I/HindIll-фрагмент ДНК плазмиды pQE31, включающий промотор/оператор фага Т5, синтетический рибосом-связывающий сайт, терминатор транскрипции фага лямбда и в качестве генетического маркера ген (β-лактамазы, детерминирующий устойчивость трансформированных плазмидой р31МС клеток бактерий E.coli к ампициллину, содержит также BamHI/Hindlll-фрагмент, кодирующий 125 аминокислотный нуклеокапсидный белок вируса репродуктивно-респираторного синдрома свиней.The recombinant plasmid DNA p31MS encoding the 125 amino acid nucleocapsid protein of the pig reproductive and respiratory syndrome virus and ensuring its expression in E. coli bacteria cells contains the BamH I / HindIll DNA fragment of the plasmid pQE31, including the T5 promoter / operator, a synthetic ribosome binding site , a lambda phage transcription terminator and, as a genetic marker, a gene (β-lactamase, which determines the resistance of ampicillin to E. coli bacteria transformed with plasmid p31MS, also contains a BamHI / Hindlll fragment, swine reproductive and respiratory syndrome virus 125 amino acid nucleocapsid protein.
RU2001105774/13A2001-03-022001-03-02Recombinant plasmid dna p31nc encoding 15 amino acid nucleocapsid protein of swine reproductive-respiratory syndrome virus and providing its expression in cells of bacterium escherichia coli
RU2205218C2
(en)
Recombinant plasmid dna p31nc encoding 15 amino acid nucleocapsid protein of swine reproductive-respiratory syndrome virus and providing its expression in cells of bacterium escherichia coli
Recombinant plasmid dna p31nc encoding 15 amino acid nucleocapsid protein of swine reproductive-respiratory syndrome virus and providing its expression in cells of bacterium escherichia coli
Recombinant plasmid dna p31nc encoding 15 amino acid nucleocapsid protein of swine reproductive-respiratory syndrome virus and providing its expression in cells of bacterium escherichia coli
Recombinant plasmid DNA p31NC encoding 125 amino acid nucleocapsid protein of the pig reproductive and respiratory syndrome virus and ensuring its expression in E. coli bacteria cells
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