RU98114360A - Recombinant plasmid DNA PUL32HCMV, which provides for the expression of a fragment of the human cytomegalovirus UL32 gene encoding the hydrophilic part of main white-brittle chicken protein - Google Patents
Recombinant plasmid DNA PUL32HCMV, which provides for the expression of a fragment of the human cytomegalovirus UL32 gene encoding the hydrophilic part of main white-brittle chicken proteinInfo
- Publication number
- RU98114360A RU98114360A RU98114360/13A RU98114360A RU98114360A RU 98114360 A RU98114360 A RU 98114360A RU 98114360/13 A RU98114360/13 A RU 98114360/13A RU 98114360 A RU98114360 A RU 98114360A RU 98114360 A RU98114360 A RU 98114360A
- Authority
- RU
- Russia
- Prior art keywords
- fragment
- pul32hcmv
- plasmid
- gene
- expression
- Prior art date
Links
- 101710031366 68 Proteins 0.000 title claims 4
- 101700067346 UL32 Proteins 0.000 title claims 4
- 229920003013 deoxyribonucleic acid Polymers 0.000 title claims 3
- 241000701024 Human betaherpesvirus 5 Species 0.000 title claims 2
- 108090000623 proteins and genes Proteins 0.000 title claims 2
- 102000004169 proteins and genes Human genes 0.000 title claims 2
- 241000287828 Gallus gallus Species 0.000 title 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N Ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 101700054771 GCA Proteins 0.000 claims 1
- 101700061402 MTRX Proteins 0.000 claims 1
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 1
- 101710017884 Segment-8 Proteins 0.000 claims 1
- 229960000723 ampicillin Drugs 0.000 claims 1
- 102000005936 beta-Galactosidase Human genes 0.000 claims 1
- 108010005774 beta-Galactosidase Proteins 0.000 claims 1
- 102000006635 beta-Lactamases Human genes 0.000 claims 1
- 108020004256 beta-Lactamases Proteins 0.000 claims 1
- 230000002068 genetic Effects 0.000 claims 1
- -1 histidine amino acid Chemical class 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 101700045377 mvp1 Proteins 0.000 claims 1
- 229920002704 polyhistidine Polymers 0.000 claims 1
- 108091007521 restriction endonucleases Proteins 0.000 claims 1
Claims (1)
EcoRV/HindIII - фрагмент ДНК (1949 п.о.) векторной плазмиды pUR290 [2], содержащий фрагмент гена LacZ, экспрессирующий только 1/3 часть β-галактозидазы (660 а.к.);
BamHI/HmdIII - фрагмент плазмиды pUR (pp 150), включающий участок гена UL32, кодирующий всю иммунодоминантную часть белка рр150 HCMV (1193 п.о.) [2];
в качестве генетического маркера ген bla β-лактамазы, определяющий устойчивость трансформированных плазмидой pUL32HCMV клеток к ампициллину;
нуклеотидную последовательность (23 п.о.), кодирующую полигистидиновый тракт в рамке считывания гена UL32, состоящий из 6 молекул аминокислоты гистидина;
уникальные сайты узнавания рестрикционными эндонуклеазами, имеющими следующие координаты: KpnI - 2082; StyI - 1741; HindIII - 2558; NruI - 1361; SacII - 2290; PvuII - 53, 1413; EcoRI - 2589Recombinant plasmid DNA pUL32HCMV, which provides the expression of the UL32 gene fragment of the human cytomegalovirus encoding the hydrophilic part of the main matrix protein pp150 HCMV, in Escherichia coli bacteria cells with a molecular weight of 2.09 megadaltons with a size of 3.165 kb, containing:
EcoRV / HindIII - DNA fragment (1949 bp) of the vector plasmid pUR290 [2] containing the LacZ gene fragment expressing only 1/3 of β-galactosidase (660 a.a.);
BamHI / HmdIII - a fragment of the plasmid pUR (pp 150), including a portion of the UL32 gene encoding the entire immunodominant portion of the HCMV pp150 protein (1193 bp) [2];
as a genetic marker, the bla β-lactamase gene, which determines the resistance of ampicillin to plasmid-transformed pUL32HCMV plasmid;
a nucleotide sequence (23 bp) encoding a polyhistidine tract in the reading frame of the UL32 gene, consisting of 6 histidine amino acid molecules;
unique recognition sites by restriction endonucleases having the following coordinates: KpnI - 2082; StyI - 1741; HindIII - 2558; NruI - 1361; SacII - 2290; PvuII - 53, 1413; EcoRI - 2589
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU98114360/13A RU2151801C1 (en) | 1998-07-28 | 1998-07-28 | Recombinant plasmid dna pul 32 hcmv determining expression of human cytomegalovirus gene ul 32 fragment encoding hydrophilic site of basic matrix protein pp 150 in escherichia coli bacterium cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU98114360/13A RU2151801C1 (en) | 1998-07-28 | 1998-07-28 | Recombinant plasmid dna pul 32 hcmv determining expression of human cytomegalovirus gene ul 32 fragment encoding hydrophilic site of basic matrix protein pp 150 in escherichia coli bacterium cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU98114360A true RU98114360A (en) | 2000-05-20 |
| RU2151801C1 RU2151801C1 (en) | 2000-06-27 |
Family
ID=20208938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU98114360/13A RU2151801C1 (en) | 1998-07-28 | 1998-07-28 | Recombinant plasmid dna pul 32 hcmv determining expression of human cytomegalovirus gene ul 32 fragment encoding hydrophilic site of basic matrix protein pp 150 in escherichia coli bacterium cells |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2151801C1 (en) |
-
1998
- 1998-07-28 RU RU98114360/13A patent/RU2151801C1/en not_active IP Right Cessation
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