RU98114356A -
Recombinant plasmid DNA PUL44HCMV, which provides for the expression of a fragment of the human cytomegalovirus UL44 gene encoding the immunomodulant part of the Black-chain-free bark-free birch bark
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Recombinant plasmid DNA PUL44HCMV, which provides for the expression of a fragment of the human cytomegalovirus UL44 gene encoding the immunomodulant part of the Black-chain-free bark-free birch bark
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RU98114356A
RU98114356ARU98114356/13ARU98114356ARU98114356ARU 98114356 ARU98114356 ARU 98114356ARU 98114356/13 ARU98114356/13 ARU 98114356/13ARU 98114356 ARU98114356 ARU 98114356ARU 98114356 ARU98114356 ARU 98114356A
Государственный научный центр вирусологии и биотехнологии "Вектор"
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Рекомбинантная плазмидная ДНК pUL44HCMV, обеспечивающая экспрессию фрагмента гена UL44 цитомегаловируса человека, кодирующего иммунодоминантную часть ДНК-связывающего белка р52(IСР36), в клетках бактерий Escherichia coli, характеризуется следующими признаками: имеет молекулярную массу 2,73 мегадальтон (4,14 т. п.о.); кодирует аминокислотную последовательность иммунодоминантной части р52 HCMV (c 200 по 431 а.к.); состоит из: BamHi/PstI - фрагмента ДНК плазмиды pQE30 (3,424 т.п.о.); BamHI/PstI - фрагмента гена UL44 (716 п.о.), кодирующего всю иммунодоминантную часть белка р52 HCMV (с 200 по 431 а.к.); содержит: в качестве генетического маркера ген bla β-лактамазы, определяющий устойчивость трансформированных плазмидой pUL44HCMV клеток к ампициллину; нуклеотидную последовательность, кодирующую полигистидиновый тракт в рамке считывания гена UL44, состоящий из 6 молекул аминокислоты гистидина, для последующей очистки с помощью аффинной хроматографии на Ni-NTA-resin; уникальные сайты узнавания рестрикционными эндонуклеазами, имеющие следующие координаты: SmaI - 498; NaeI - 475; NcoI - 530; BamHI - 146; PstI - 862.The recombinant plasmid DNA pUL44HCMV, which provides expression of the UL44 gene fragment of the human cytomegalovirus encoding the immunodominant part of the p52 DNA-binding protein (ICP36), in Escherichia coli bacteria cells, is characterized by the following characteristics: it has a molecular weight of 2.73 megadaltons (4.14 t. about.); encodes the amino acid sequence of the immunodominant part of p52 HCMV (c 200 to 431 a.a.); consists of: BamHi / PstI - DNA fragment of plasmid pQE30 (3,424 kb); BamHI / PstI — fragment of the UL44 gene (716 bp) encoding the entire immunodominant portion of the p52 HCMV protein (200 to 431 a.a.); contains: as a genetic marker, the bla gene of β-lactamase, which determines the resistance of ampicillin to cells transformed by the pUL44HCMV plasmid; a nucleotide sequence encoding a polyhistidine tract in the reading frame of the UL44 gene, consisting of 6 histidine amino acid molecules, for subsequent purification using affinity chromatography on Ni-NTA-resin; unique recognition sites by restriction endonucleases having the following coordinates: SmaI - 498; NaeI 475; NcoI - 530; BamHI - 146; PstI - 862.
RU98114356/13A1998-07-281998-07-28Recombinant plasmid dna pul 44 hcmv determining expression of human cytomegalovirus gene ul 44 fragment encoding immunodominant site of dna-binding protein p 52 (icp 36) in escherichia coli bacterium cells
RU2151800C1
(en)
Recombinant plasmid dna pul 44 hcmv determining expression of human cytomegalovirus gene ul 44 fragment encoding immunodominant site of dna-binding protein p 52 (icp 36) in escherichia coli bacterium cells
Recombinant plasmid dna pul 44 hcmv determining expression of human cytomegalovirus gene ul 44 fragment encoding immunodominant site of dna-binding protein p 52 (icp 36) in escherichia coli bacterium cells
Recombinant plasmid dna pul 44 hcmv determining expression of human cytomegalovirus gene ul 44 fragment encoding immunodominant site of dna-binding protein p 52 (icp 36) in escherichia coli bacterium cells
Circular dna molecule with conditional origin of replication which contains nucleic acid of interest and selection marker but does not encode its replication initiation protein, method for preparing the same and use thereof in gene therapy
Recombinant plasmid DNA PUL44HCMV, which provides for the expression of a fragment of the human cytomegalovirus UL44 gene encoding the immunomodulant part of the Black-chain-free bark-free birch bark
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