RU2003123534A -
RECOMBINANT PLASMID DNA PET23-A (+) PRXVIHUMΔ178, CODING THE N-TERM HUMAN PEROXYREDOXINE VI AND E. coli BL21 / DE3 / PET23-PENCIUM
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RECOMBINANT PLASMID DNA PET23-A (+) PRXVIHUMΔ178, CODING THE N-TERM HUMAN PEROXYREDOXINE VI AND E. coli BL21 / DE3 / PET23-PENCIUM
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RU2003123534A
RU2003123534ARU2003123534/13ARU2003123534ARU2003123534ARU 2003123534 ARU2003123534 ARU 2003123534ARU 2003123534/13 ARU2003123534/13 ARU 2003123534/13ARU 2003123534 ARU2003123534 ARU 2003123534ARU 2003123534 ARU2003123534 ARU 2003123534A
Институт биоорганической химии им. академиков М.М.Шем кина и Ю.А.Овчинникова Российской академии наук (RU)
Институт биоорганической химии им. академиков М.М.Шемякина и Ю.А.Овчинникова Российской академии наук
Институт биофизики клетки РАН (RU)
Институт биофизики клетки РАН
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Application filed by Институт биоорганической химии им. академиков М.М.Шем кина и Ю.А.Овчинникова Российской академии наук (RU), Институт биоорганической химии им. академиков М.М.Шемякина и Ю.А.Овчинникова Российской академии наук, Институт биофизики клетки РАН (RU), Институт биофизики клетки РАНfiledCriticalИнститут биоорганической химии им. академиков М.М.Шем кина и Ю.А.Овчинникова Российской академии наук (RU)
Priority to RU2003123534/13ApriorityCriticalpatent/RU2250262C1/en
Priority to PCT/RU2003/000473prioritypatent/WO2004043485A1/en
Priority to US10/534,238prioritypatent/US8003345B2/en
Priority to JP2005506685Aprioritypatent/JP2006505290A/en
Priority to CA002505478Aprioritypatent/CA2505478A1/en
Priority to EP03774414Aprioritypatent/EP1566182A4/en
Publication of RU2003123534ApublicationCriticalpatent/RU2003123534A/en
Application grantedgrantedCritical
Publication of RU2250262C1publicationCriticalpatent/RU2250262C1/en
Micro-Organisms Or Cultivation Processes Thereof
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Claims (2)
1. Рекомбинантная плазмидная ДНК рЕТ23-a(+)/PrxVIhumΔ178, кодирующая N-концевой фрагмент пероксиредоксина VI человека с молекулярной массой 19691,61 Да, содержащая EcoRI-NdeI-фрагмент плазмиды рЕТ23-а(+), включающий промотор РНК-полимеразы фага Т7, участок инициации репликации (ori), генетический маркер (Ар), детерминирующий устойчивость трансформированных плазмидой pET23-a(+)/PrxVIhumΔ178 клеток E.coli к ампициллину, уникальные сайты узнавания рестрикционными эндонуклеазами со следующими координатами: NdeI-790, EcoRI-192, PvuII-1531 и NdeI-EcoRI - фрагмент гена PrxVIhum длиной 552 п.о., кодирующий PrxVIhumΔ178.1. Recombinant plasmid DNA pET23-a (+) / PrxVIhumΔ178 encoding the N-terminal fragment of human peroxyredoxin VI with a molecular weight of 19691.61 Da, containing EcoRI-NdeI fragment of the plasmid pET23-a (+), including the phage RNA polymerase promoter T7, replication initiation site (ori), genetic marker (Ap), which determines the resistance of ampicillin to E. coli transformed with plasmid pET23-a (+) / PrxVIhumΔ178, unique recognition sites by restriction endonucleases with the following coordinates: NdeI-790, EcoRI-192 , PvuII-1531 and NdeI-EcoRI - 552 bp PrxVIhum gene fragment encoding PrxVIhumΔ178.2. Штамм Е.coli BL21/DE3/pET23-a(+)/PnxVIhumΔ178 - продуцент N-концевого фрагмента пероксиредоксина VI человека.2. Strain E. coli BL21 / DE3 / pET23-a (+) / PnxVIhumΔ178 - producer of the N-terminal fragment of human peroxyredoxin VI.
RU2003123534/13A2002-11-102003-07-29RECOMBINANT PLASMID DNA pET23-a(+)/PrxVIhumΔ178, ENCODING N-TERMINAL FRAGMENT OF HUMAN PEROXIREDOXINE VI, AND E.coli STRAIN BL21/DE3/pET23-a(+)/PrxVIhumΔ178 AS PRODUCER OF N-TERMINAL FRAGMENT OF HUMAN PEROXIREDOXINE VI
RU2250262C1
(en)
RECOMBINANT PLASMID DNA pET23-a(+)/PrxVIhumΔ178, ENCODING N-TERMINAL FRAGMENT OF HUMAN PEROXIREDOXINE VI, AND E.coli STRAIN BL21/DE3/pET23-a(+)/PrxVIhumΔ178 AS PRODUCER OF N-TERMINAL FRAGMENT OF HUMAN PEROXIREDOXINE VI
RECOMBINANT PLASMID DNA pET23-a(+)/PrxVIhumΔ178, ENCODING N-TERMINAL FRAGMENT OF HUMAN PEROXIREDOXINE VI, AND E.coli STRAIN BL21/DE3/pET23-a(+)/PrxVIhumΔ178 AS PRODUCER OF N-TERMINAL FRAGMENT OF HUMAN PEROXIREDOXINE VI
RECOMBINANT PLASMID DNA pET23-a(+)/PrxVIhumΔ178, ENCODING N-TERMINAL FRAGMENT OF HUMAN PEROXIREDOXINE VI, AND E.coli STRAIN BL21/DE3/pET23-a(+)/PrxVIhumΔ178 AS PRODUCER OF N-TERMINAL FRAGMENT OF HUMAN PEROXIREDOXINE VI
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