RU2008106110A - Recombinant plasmid DNA rFastBac-G2R-IgG, comprising a fragment variola virus genomes encoding the tumor necrosis factor binding protein, and fragments of the human genome encodes a heavy chain of immunoglobulin G, and a strain of baculovirus BVG2RIgG, producing mouse soluble chimeric tumor necrosis factor binding protein variola virus Smallpox with a fragment of the heavy chain of human immunoglobulin G - Google Patents
Recombinant plasmid DNA rFastBac-G2R-IgG, comprising a fragment variola virus genomes encoding the tumor necrosis factor binding protein, and fragments of the human genome encodes a heavy chain of immunoglobulin G, and a strain of baculovirus BVG2RIgG, producing mouse soluble chimeric tumor necrosis factor binding protein variola virus Smallpox with a fragment of the heavy chain of human immunoglobulin G Download PDFInfo
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- RU2008106110A RU2008106110A RU2008106110/13A RU2008106110A RU2008106110A RU 2008106110 A RU2008106110 A RU 2008106110A RU 2008106110/13 A RU2008106110/13 A RU 2008106110/13A RU 2008106110 A RU2008106110 A RU 2008106110A RU 2008106110 A RU2008106110 A RU 2008106110A
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Abstract
1. Рекомбинантная плазмидная ДНК pFastBac-G2R-IgG размером 6444 п.н. и молекулярной массой 4,18 мДа, несущая фрагмент генома вируса натуральной оспы штамма India-1967, кодирующий растворимый фактор некроза опухолей связывающий белок, и фрагмент генома человека, кодирующий фрагмент тяжелой цепи иммуноглобулина G человека, содержащая: ! ПЦР-фрагмент генома вируса натуральной оспы штамма India-67 длиной 1068 п.н., кодирующий ФНО-связывающий белок без стоп-кодона, полученный с использованием праймеров ! 1) 5' CGGGATCCCTACATTATTAAATCATGAAGTCCG 3' ! 2) 5' GCTCTAGATAAAAAGCGGGTGGGTTTGG 3', ! фланкированный сайтами узнавания эндонуклеаз рестрикции BamHI и ХbаI; ! фрагмент генома человека длиной 699 п.н., кодирующий фрагмент тяжелой цепи IgG1 человека с 117 по 329 а.к., полученный из плазмиды pBluescript-IgG1 после гидролиза эндонуклеазами рестрикции ХbаI и HindIII; ! ВаmHI - HindIII фрагмент векторной плазмиды pFastBac, размером 4677 п.н., включающий бакуловирусный промотор pPolh, мини-Тn7-транспазон и сигнал для полиаденилирования вируса SV-40, обеспечивающий сайт-специфическую транспозицию ДНК химерного гена G2R-IgG в геном бакуловируса; ! генетические маркеры: ! ген α-лактамазы, определяющий устойчивость к ампициллину; ! ген аминогликозидтрансферазы, определяющий устойчивость к гентамицину; ! уникальные сайты рестрикции: ВаmHI (4033), HindIII (5806). ! 2. Штамм рекомбинантного бакуловируса BvG2RIgG, полученный с помощью рекомбинантной плазмидной ДНК pFastBac-G2R-IgG по п.1, депонированный в Коллекции микроорганизмов ФГУН ГНЦ ВБ «Вектор» Роспотребнадзора под номером V-354, - продуцент растворимого химерного фактор некроза опухолей связывающего белка вируса натуральной оспы с фрагментом тяжелой цепи иммуноглобулина G человека.1. Recombinant plasmid DNA pFastBac-G2R-IgG size 6444 bp and a molecular weight of 4.18 mDa, carrying a fragment of the genome of the smallpox virus of strain India-1967, encoding a soluble tumor necrosis factor binding protein, and a fragment of the human genome encoding a fragment of the human immunoglobulin G heavy chain, containing:! PCR fragment of the genome of smallpox virus strain India-67 1068 bp in length, encoding a TNF-binding protein without a stop codon, obtained using primers! 1) 5 'CGGGATCCCTACATTATTAAATCATGAAGTCCG 3'! 2) 5 'GCTCTAGATAAAAAGCGGGTGGGTTTGG 3',! flanked by recognition sites of restriction endonucleases BamHI and XbaI; ! a fragment of the human genome 699 bp long, encoding a fragment of the heavy chain of human IgG1 from 117 to 329 a.a., obtained from the plasmid pBluescript-IgG1 after hydrolysis with restriction endonucleases XbaI and HindIII; ! BamHI - HindIII fragment of the vector plasmid pFastBac, size 4677 bp, including the baculovirus promoter pPolh, mini-Tn7 transposon and a signal for polyadenylation of the SV-40 virus, providing site-specific transposition of DNA of the chimeric gene G2R-IgG into the baculovirus genome; ! genetic markers:! α-lactamase gene, which determines ampicillin resistance; ! aminoglycoside transferase gene, which determines resistance to gentamicin; ! unique restriction sites: BamHI (4033), HindIII (5806). ! 2. The strain of recombinant baculovirus BvG2RIgG obtained using the recombinant plasmid DNA pFastBac-G2R-IgG according to claim 1, deposited in the Collection of microorganisms of the Federal State Institution of Science and Science of the World Bank "Vector" Rospotrebnadzor under the number V-354, is the producer of soluble chimeric tumor necrosis protein binding factor smallpox with a fragment of the heavy chain of human immunoglobulin G.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008106110/13A RU2376375C2 (en) | 2008-02-18 | 2008-02-18 | RECOMBINANT PLASMID DNA pFastBac-G2R-IgG, WHICH CONTAINS FRAGMENT OF SMALLPOX VIRUS GENOME, CODING FACTOR OF TUMOUR NECROSIS, BINDING PROTEIN, AND FRAGMENT OF HUMAN GENOME, CODING SECTION OF HEAVY CHAIN OF ANTIBODY G, AND STRAIN OF BACULOVIRUS BvG2RIgG, WHICH PRODUCES SOLUBLE CHIMERIC PROTEIN, WHICH CONSISTS OF SMALLPOX VIRUS PROTEIN, BINDING FACTOR OF TUMOUR NECROSIS, AND FRAGMENT OF HEAVY CHAIN OF HUMAN ANTIBODY G |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008106110/13A RU2376375C2 (en) | 2008-02-18 | 2008-02-18 | RECOMBINANT PLASMID DNA pFastBac-G2R-IgG, WHICH CONTAINS FRAGMENT OF SMALLPOX VIRUS GENOME, CODING FACTOR OF TUMOUR NECROSIS, BINDING PROTEIN, AND FRAGMENT OF HUMAN GENOME, CODING SECTION OF HEAVY CHAIN OF ANTIBODY G, AND STRAIN OF BACULOVIRUS BvG2RIgG, WHICH PRODUCES SOLUBLE CHIMERIC PROTEIN, WHICH CONSISTS OF SMALLPOX VIRUS PROTEIN, BINDING FACTOR OF TUMOUR NECROSIS, AND FRAGMENT OF HEAVY CHAIN OF HUMAN ANTIBODY G |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2008106110A true RU2008106110A (en) | 2009-08-27 |
| RU2376375C2 RU2376375C2 (en) | 2009-12-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| RU2008106110/13A RU2376375C2 (en) | 2008-02-18 | 2008-02-18 | RECOMBINANT PLASMID DNA pFastBac-G2R-IgG, WHICH CONTAINS FRAGMENT OF SMALLPOX VIRUS GENOME, CODING FACTOR OF TUMOUR NECROSIS, BINDING PROTEIN, AND FRAGMENT OF HUMAN GENOME, CODING SECTION OF HEAVY CHAIN OF ANTIBODY G, AND STRAIN OF BACULOVIRUS BvG2RIgG, WHICH PRODUCES SOLUBLE CHIMERIC PROTEIN, WHICH CONSISTS OF SMALLPOX VIRUS PROTEIN, BINDING FACTOR OF TUMOUR NECROSIS, AND FRAGMENT OF HEAVY CHAIN OF HUMAN ANTIBODY G |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2471869C1 (en) * | 2011-05-10 | 2013-01-10 | Федеральное бюджетное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор" (ФБУН ГНЦ ВБ "Вектор") | RECOMBINANT PLASMID DNA pQE-60-TNFR-CrmB-Ind-67 AND pFastBac1-G2R-dSECRET CONTAINING SMALLPOX VIRUS GENOME FRAGMENT CODING TUMOUR NECROSIS FACTOR BINDING CrmB PROTEIN DOMAIN AND Bv/G2R-dSECRET BACULOVIRUS DOMAIN PRODUCING SECRETING TNF-BINDING CrmB PROTEIN OF SMALLPOX VIRUS WITH DELETED SECRET DOMAIN |
| RU2531547C1 (en) * | 2013-03-04 | 2014-10-20 | Федеральное государственное бюджетное учреждение науки Институт биорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) | Recombinant plasmid dna pet3.54, encoding polypeptide fn3.54, interacting with human tumour necrosis factor, and strain of bacteria escherichia coli - producer of polypeptide fn3.54, interacting with human tumour necrosis factor |
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Effective date: 20170219 |