RU2011118764A - Recombinant plasmid DNA of pQE-60-TNFR-CrmB-Ind-67 and pFastBac1-G2R-dSECRET, containing units of variola virus genomes encoding the tumor necrosis factor binding domain PROTEIN CrmB AND STRAIN baculovirus BV / G2R-dSECRET, producing a secreted TNF-binding PROTEIN VIRUS CRMB PROTEIN WITH DELETED SECRET DOMAIN - Google Patents
Recombinant plasmid DNA of pQE-60-TNFR-CrmB-Ind-67 and pFastBac1-G2R-dSECRET, containing units of variola virus genomes encoding the tumor necrosis factor binding domain PROTEIN CrmB AND STRAIN baculovirus BV / G2R-dSECRET, producing a secreted TNF-binding PROTEIN VIRUS CRMB PROTEIN WITH DELETED SECRET DOMAIN Download PDFInfo
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- RU2011118764A RU2011118764A RU2011118764/10A RU2011118764A RU2011118764A RU 2011118764 A RU2011118764 A RU 2011118764A RU 2011118764/10 A RU2011118764/10 A RU 2011118764/10A RU 2011118764 A RU2011118764 A RU 2011118764A RU 2011118764 A RU2011118764 A RU 2011118764A
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Abstract
1. Рекомбинантная плазмидная ДНК pFastBac1-G2R-dSECRET размером 5285 п.н. и молекулярной массой 3,43 мДа, несущая фрагмент генома вируса натуральной оспы штамма India-1967, кодирующий ФНО-связывающий домен белка CrmB, содержащая: ! - ПЦР-фрагмент генома вируса натуральной оспы штамма India-67 длиной 525 п.н., кодирующий ФНО-связывающий домен белка CrmB ВНО (штамм India-1967), полученный с помощью праймеров ! 5' CGAGGATCCGCCACCATGAAGTCCGTATTATACTTGTATATATTG 3' ! 5' CGGAATTCCTAGGGTACGGGTTCGCATTTAT 3' ! фланкированный сайтами узнавания эндонуклеаз рестрикции EcoRI и BamHI; ! - EcoRI - BamHI фрагмент векторной плазмиды pFastBac1, размером 4760 п.н п.н., включающий бакуловирусный промотор pPolh, мини-Tn7-транспазон и сигнал для полиаденилирования вируса SV-40. ! 2. Штамм рекомбинантного бакуловируса Bv/G2R-dSECRET, полученный с помощью рекомбинантной плазмидной ДНК pFastBac1-G2R-dSECRET по п.1, депонированный в коллекции микроорганизмов ФГУН ГНЦ ВБ «Вектор» Роспотребнадзора под номером V-537 - продуцент ФНО-связывающего домена белка CrmB ВНО (штамм India-1967). ! 3. Рекомбинантная плазмидная ДНК pQE-60-TNFR-CrmB-Ind-67 размером 3872 п.н. и молекулярной массой 2,5 мДа, депонированная в коллекции микроорганизмов ФГУН ГНЦ ВБ «Вектор» Роспотребнадзора под номером P-48 и несущая фрагмент генома вируса натуральной оспы штамма India-1967, кодирующий ФНО-связывающий домен белка CrmB, содержащая: ! - ПЦР-фрагмент генома вируса натуральной оспы штамма India-67 длиной 453 п.н., кодирующий ФНО-связывающий домен белка CrmB ВНО (штамм India-1967), полученный с помощью праймеров !5´ CGCCATGGCACCGTATACACCACCCAATGGA 3´ ! 5´ CGAGATCTGGGTACGGGTTCGCATTTATCTG 3´ ! фланкированный сайтами узнавания эндонуклеаз рестрикции NcoI и BglII; ! - NcoI - BglII фрагмент векторной плазмиды pFastBac1, размером 3419 п.н., включающий ген β-лактамазы, определяющий уст 1. Recombinant plasmid DNA pFastBac1-G2R-dSECRET size 5285 BP and a molecular weight of 3.43 mDa, carrying a fragment of the genome of smallpox virus strain India-1967, encoding the TNF-binding domain of the CrmB protein, containing:! - PCR fragment of the genome of smallpox virus strain India-67 525 bp long, encoding the TNF-binding domain of the protein CrmB VNO (strain India-1967), obtained using primers! 5 'CGAGGATCCGCCACCATGAAGTCCGTATTATACTTGTATATATTG 3'! 5 'CGGAATTCCTAGGGTACGGGTTGGCATTTAT 3'! flanked by recognition sites of restriction endonucleases EcoRI and BamHI; ! - EcoRI - BamHI fragment of the vector plasmid pFastBac1, size 4760 bp, including the baculovirus promoter pPolh, mini-Tn7-transposon and signal for polyadenylation of the SV-40 virus. ! 2. The strain of recombinant baculovirus Bv / G2R-dSECRET, obtained using the recombinant plasmid DNA pFastBac1-G2R-dSECRET according to claim 1, deposited in the collection of microorganisms FSHC SSC WB "Vector" Rospotrebnadzor under number V-537 - producer of the protein of the TNF-binding CrmB VNO (strain India-1967). ! 3. Recombinant plasmid DNA pQE-60-TNFR-CrmB-Ind-67 with a size of 3872 bp and a molecular weight of 2.5 mDa, deposited in the collection of microorganisms of the Federal State Institution of Science and Science of the Scientific Center "Vector" of Rospotrebnadzor under the number P-48 and carrying a fragment of the genome of smallpox virus strain India-1967, encoding the TNF-binding domain of the CrmB protein, containing:! - PCR fragment of the genome of smallpox virus strain India-67 453 bp long, encoding the TNF-binding domain of the CrmB VNO protein (strain India-1967) obtained using primers! 5´ CGCCATGGCACCGTATACACCACCCAATGGA 3´! 5´ CGAGATCTGGGTACGGGTTCGCATTTATCTG 3´! flanked by recognition sites of restriction endonucleases NcoI and BglII; ! - NcoI - BglII fragment of the vector plasmid pFastBac1, size 3419 bp, including the β-lactamase gene, which determines the
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2011118764/10A RU2471869C1 (en) | 2011-05-10 | 2011-05-10 | RECOMBINANT PLASMID DNA pQE-60-TNFR-CrmB-Ind-67 AND pFastBac1-G2R-dSECRET CONTAINING SMALLPOX VIRUS GENOME FRAGMENT CODING TUMOUR NECROSIS FACTOR BINDING CrmB PROTEIN DOMAIN AND Bv/G2R-dSECRET BACULOVIRUS DOMAIN PRODUCING SECRETING TNF-BINDING CrmB PROTEIN OF SMALLPOX VIRUS WITH DELETED SECRET DOMAIN |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2011118764/10A RU2471869C1 (en) | 2011-05-10 | 2011-05-10 | RECOMBINANT PLASMID DNA pQE-60-TNFR-CrmB-Ind-67 AND pFastBac1-G2R-dSECRET CONTAINING SMALLPOX VIRUS GENOME FRAGMENT CODING TUMOUR NECROSIS FACTOR BINDING CrmB PROTEIN DOMAIN AND Bv/G2R-dSECRET BACULOVIRUS DOMAIN PRODUCING SECRETING TNF-BINDING CrmB PROTEIN OF SMALLPOX VIRUS WITH DELETED SECRET DOMAIN |
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| Publication Number | Publication Date |
|---|---|
| RU2011118764A true RU2011118764A (en) | 2012-11-20 |
| RU2471869C1 RU2471869C1 (en) | 2013-01-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| RU2011118764/10A RU2471869C1 (en) | 2011-05-10 | 2011-05-10 | RECOMBINANT PLASMID DNA pQE-60-TNFR-CrmB-Ind-67 AND pFastBac1-G2R-dSECRET CONTAINING SMALLPOX VIRUS GENOME FRAGMENT CODING TUMOUR NECROSIS FACTOR BINDING CrmB PROTEIN DOMAIN AND Bv/G2R-dSECRET BACULOVIRUS DOMAIN PRODUCING SECRETING TNF-BINDING CrmB PROTEIN OF SMALLPOX VIRUS WITH DELETED SECRET DOMAIN |
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Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2241754C2 (en) * | 2002-10-31 | 2004-12-10 | Государственный научный центр вирусологии и биотехнологии "Вектор" | Recombinant plasmid dna pfastbac-g2r comprising smallpox virus genome fragment encoding protein-analog of tumor necrosis factor receptor and strain of baculovirus btri67 producing soluble receptor of smallpox virus tumor necrosis factor |
| ES2315037B1 (en) * | 2004-09-02 | 2009-12-29 | Antonio Alcami Pertejo | CHEMIOKIN UNION ACTIVITY CODIFIED BY TNF RECEPTORS AND RELATED PROTEINS. |
| RU2376375C2 (en) * | 2008-02-18 | 2009-12-20 | Федеральное государственное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека, ФГУН ГНЦ ВБ "Вектор" Роспотребнадзора | RECOMBINANT PLASMID DNA pFastBac-G2R-IgG, WHICH CONTAINS FRAGMENT OF SMALLPOX VIRUS GENOME, CODING FACTOR OF TUMOUR NECROSIS, BINDING PROTEIN, AND FRAGMENT OF HUMAN GENOME, CODING SECTION OF HEAVY CHAIN OF ANTIBODY G, AND STRAIN OF BACULOVIRUS BvG2RIgG, WHICH PRODUCES SOLUBLE CHIMERIC PROTEIN, WHICH CONSISTS OF SMALLPOX VIRUS PROTEIN, BINDING FACTOR OF TUMOUR NECROSIS, AND FRAGMENT OF HEAVY CHAIN OF HUMAN ANTIBODY G |
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