RU2002129177A - Recombinant plasmid DNA PFASTBAC-G2R, containing units of variola virus genomes protein coding ANALOG TUMOR NECROSIS FACTOR RECEPTOR AND STRAIN baculovirus BTRI67, producing SOLUBLE TUMOR NECROSIS FACTOR RECEPTOR VARIOLA VIRUS - Google Patents

Recombinant plasmid DNA PFASTBAC-G2R, containing units of variola virus genomes protein coding ANALOG TUMOR NECROSIS FACTOR RECEPTOR AND STRAIN baculovirus BTRI67, producing SOLUBLE TUMOR NECROSIS FACTOR RECEPTOR VARIOLA VIRUS

Info

Publication number
RU2002129177A
RU2002129177A RU2002129177/13A RU2002129177A RU2002129177A RU 2002129177 A RU2002129177 A RU 2002129177A RU 2002129177/13 A RU2002129177/13 A RU 2002129177/13A RU 2002129177 A RU2002129177 A RU 2002129177A RU 2002129177 A RU2002129177 A RU 2002129177A
Authority
RU
Russia
Prior art keywords
necrosis factor
tumor necrosis
factor receptor
pfastbac
variola virus
Prior art date
Application number
RU2002129177/13A
Other languages
Russian (ru)
Other versions
RU2241754C2 (en
Inventor
Ирина Павловна Гилева
Сергей Николаевич Щелкунов
Игорь Александрович Рязанкин
Заки Амирович Максютов
Алексей Викторович Тотменин
Андрей Егорович Нестеров
Валентина Александровна Агеенко
Original Assignee
Государственный научный центр вирусологии и биотехнологии "Вектор"
Filing date
Publication date
Application filed by Государственный научный центр вирусологии и биотехнологии "Вектор" filed Critical Государственный научный центр вирусологии и биотехнологии "Вектор"
Priority to RU2002129177/13A priority Critical patent/RU2241754C2/en
Priority claimed from RU2002129177/13A external-priority patent/RU2241754C2/en
Publication of RU2002129177A publication Critical patent/RU2002129177A/en
Application granted granted Critical
Publication of RU2241754C2 publication Critical patent/RU2241754C2/en

Links

Claims (2)

1. Рекомбинантная плазмидная ДНК pFastBac-G2R, несущая фрагмент генома вируса натуральной оспы, кодирующий растворимый белок-аналог рецептора ФНОα человека, размером 5836 п.н. и молекулярной массой 3,85 Мда, содержащая ПЦР-фрагмент генома вируса натуральной оспы штамма Индия-1967, содержащий ген G2R, кодирующий белок-аналог рецептора фактора некроза опухолей человека, фланкированный сайтами узнавания эндонуклеаз рестрикции BamHI и EcoRI, размером 1084 п.о.; BamHI-EcoRI фрагмент векторной плазмиды pFastBac, размером 4752п.н., включающий бакуловирусный промотор pPolh, мини-Tn7-транспазон и сигнал для полиаденилирования вируса SV-40, обеспечивающий сайт-специфическую транспозицию ДНК гена G2R в геном бакуловируса; генетические маркеры: ген β-лактамазы, определяющий устойчивость к ампициллину; ген аминогликозидтрансферазы, определяющий устойчивость к гентамицину; уникальные сайты рестрикции: BamHI (4032); Dral (4801); EcoRI (5116).1. Recombinant plasmid DNA pFastBac-G2R, carrying a fragment of the genome of smallpox virus, encoding a soluble protein analogue of human TNFα receptor, size 5836 bp and a molecular weight of 3.85 Mda, containing a PCR fragment of the genome of smallpox virus strain India-1967, containing the G2R gene, encoding a protein analogue of the receptor for necrosis factor of human tumors, flanked by recognition sites of restriction endonucleases BamHI and EcoRI, 1084 bp. ; BamHI-EcoRI fragment of the 4752 bp vector plasmid pFastBac, including the pPolh baculovirus promoter, mini-Tn7 transposon and the SV-40 virus polyadenylation signal enabling site-specific transposition of G2R gene DNA into the baculovirus genome; genetic markers: β-lactamase gene, which determines ampicillin resistance; aminoglycoside transferase gene, which determines resistance to gentamicin; unique restriction sites: BamHI (4032); Dral (4801); EcoRI (5116). 2. Штамм рекомбинантного бакуловируса BTRi67, полученный с помощью рекомбинантной плазмидной ДНК pFastBac-G2R по п.1, депонированный в НИИ ККМ под номером VB-02, - продуцент растворимого белка-аналога рецептора ФНОα человека, кодируемого геном G2R вируса натуральной оспы.2. The strain of recombinant baculovirus BTRi67, obtained using the recombinant plasmid DNA pFastBac-G2R according to claim 1, deposited in the KKM Research Institute under the number VB-02, is a producer of soluble human TNFα receptor analog protein encoded by the smallpox virus G2R gene.
RU2002129177/13A 2002-10-31 2002-10-31 Recombinant plasmid dna pfastbac-g2r comprising smallpox virus genome fragment encoding protein-analog of tumor necrosis factor receptor and strain of baculovirus btri67 producing soluble receptor of smallpox virus tumor necrosis factor RU2241754C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2002129177/13A RU2241754C2 (en) 2002-10-31 2002-10-31 Recombinant plasmid dna pfastbac-g2r comprising smallpox virus genome fragment encoding protein-analog of tumor necrosis factor receptor and strain of baculovirus btri67 producing soluble receptor of smallpox virus tumor necrosis factor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2002129177/13A RU2241754C2 (en) 2002-10-31 2002-10-31 Recombinant plasmid dna pfastbac-g2r comprising smallpox virus genome fragment encoding protein-analog of tumor necrosis factor receptor and strain of baculovirus btri67 producing soluble receptor of smallpox virus tumor necrosis factor

Publications (2)

Publication Number Publication Date
RU2002129177A true RU2002129177A (en) 2004-07-27
RU2241754C2 RU2241754C2 (en) 2004-12-10

Family

ID=34387242

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2002129177/13A RU2241754C2 (en) 2002-10-31 2002-10-31 Recombinant plasmid dna pfastbac-g2r comprising smallpox virus genome fragment encoding protein-analog of tumor necrosis factor receptor and strain of baculovirus btri67 producing soluble receptor of smallpox virus tumor necrosis factor

Country Status (1)

Country Link
RU (1) RU2241754C2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2471869C1 (en) * 2011-05-10 2013-01-10 Федеральное бюджетное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор" (ФБУН ГНЦ ВБ "Вектор") RECOMBINANT PLASMID DNA pQE-60-TNFR-CrmB-Ind-67 AND pFastBac1-G2R-dSECRET CONTAINING SMALLPOX VIRUS GENOME FRAGMENT CODING TUMOUR NECROSIS FACTOR BINDING CrmB PROTEIN DOMAIN AND Bv/G2R-dSECRET BACULOVIRUS DOMAIN PRODUCING SECRETING TNF-BINDING CrmB PROTEIN OF SMALLPOX VIRUS WITH DELETED SECRET DOMAIN

Similar Documents

Publication Publication Date Title
Howard et al. Vaccinia virus homologues of the Shope fibroma virus inverted terminal repeat proteins and a discontinuous ORF related to the tumor necrosis factor receptor family
ES2256776T3 (en) INTERGENIC REGIONS AS INSERTION SITES IN THE GENOMA OF THE ANKARA VACCINIA MODIFIED VIRUS (MVA)
Giri et al. Genomic structure of the cottontail rabbit (Shope) papillomavirus.
CN1297479A (en) Modified adenovirus contg. fiber replacement protein
EP3664841A1 (en) Oncolytic virus expressing a car t cell target and uses thereof
JPH07285997A (en) Tnf ectopic protein
JP2020510426A5 (en)
CA2384827A1 (en) Capsid-modified recombinant adenovirus and methods of use
EP3274363A1 (en) Anti-tumour medicament based on adenovirus
CN111936622A (en) Recombinant vaccinia virus and pharmaceutical composition comprising same
CN108840947A (en) Bovine albumin-interferon-' alpha '-interleukin-22 fusion protein, preparation method and its encoding gene, a kind of ox long-acting interferon
RU2002129177A (en) Recombinant plasmid DNA PFASTBAC-G2R, containing units of variola virus genomes protein coding ANALOG TUMOR NECROSIS FACTOR RECEPTOR AND STRAIN baculovirus BTRI67, producing SOLUBLE TUMOR NECROSIS FACTOR RECEPTOR VARIOLA VIRUS
JP2004536607A (en) Viral variants that selectively replicate in targeted human cancer cells
JP6235697B2 (en) Mutant vaccinia virus strains, their use and methods of making them
RU2008106110A (en) Recombinant plasmid DNA rFastBac-G2R-IgG, comprising a fragment variola virus genomes encoding the tumor necrosis factor binding protein, and fragments of the human genome encodes a heavy chain of immunoglobulin G, and a strain of baculovirus BVG2RIgG, producing mouse soluble chimeric tumor necrosis factor binding protein variola virus Smallpox with a fragment of the heavy chain of human immunoglobulin G
RU2006113756A (en) Recombinant plasmid DNA pFastBac-B9RZ, VIRUS GENOME FRAGMENT CONTAINING monkeypox encoding interferon gamma binding protein, and a strain of baculovirus BvB9RZ, producing mouse soluble interferon-gamma binding protein VIRUS monkeypox
JP2007511534A5 (en)
Seregin et al. Comparative studies of gamma-interferon receptor-like proteins of variola major and variola minor viruses
CN108822220A (en) Sheep albumin-interferon-tau-interleukin-22 fusion protein, preparation method and its encoding gene, a kind of sheep long-acting interferon
CN108840921B (en) Sheep alpha interferon mutant and preparation method and application thereof
EP1339737B1 (en) Immunomodulatory protein derived from the yaba monkey tumor virus
RU2003123534A (en) RECOMBINANT PLASMID DNA PET23-A (+) PRXVIHUMΔ178, CODING THE N-TERM HUMAN PEROXYREDOXINE VI AND E. coli BL21 / DE3 / PET23-PENCIUM
EP1236800A3 (en) Nucleic acids encoding interferon alpha 2, containing single nucleotide polymorphisms and methods of use thereof
AU2002215727A1 (en) Immunomodulatory protein derived from the yaba monkey tumor virus
Benes̆ et al. Nucleotide sequence of the right early region of Bacillus phage φ15 and comparison with related phages: reorganization of gene 17 during evolution