RU2006140996A -
Recombinant plasmid DNA pTrcTREN-IL-13, which encodes a polypeptide with the properties of human interleukin-13, and the strain of Escherichia coli BL21 (DE3) / pTrcTREN-SUMMER
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Recombinant plasmid DNA pTrcTREN-IL-13, which encodes a polypeptide with the properties of human interleukin-13, and the strain of Escherichia coli BL21 (DE3) / pTrcTREN-SUMMER
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RU2006140996A
RU2006140996ARU2006140996/13ARU2006140996ARU2006140996ARU 2006140996 ARU2006140996 ARU 2006140996ARU 2006140996/13 ARU2006140996/13 ARU 2006140996/13ARU 2006140996 ARU2006140996 ARU 2006140996ARU 2006140996 ARU2006140996 ARU 2006140996A
Институт биоорганической химии им. академиков М.М. Шем кина и Ю.А. Овчинникова Российской Академии Наук (RU)
Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук
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1. Рекомбинантная плазмидная ДНК pTrcTREN-IL13 с молекулярной массой 2,83 Md (4,579 т.п.о.), обеспечивающая биосинтез полипептида со свойствами интерлейкина-13 человека в клетках Е. coli и состоящая из1. Recombinant plasmid DNA pTrcTREN-IL13 with a molecular weight of 2.83 Md (4.579, etc., etc.), providing biosynthesis of a polypeptide with the properties of human interleukin-13 in E. coli cells and consisting ofClaI/HindIII - фрагмента ДНК плазмиды pTrcTEGF длиной 4,232 т.п.о., включающего trc-промотор Е. coli, синтетический усилитель трансляции TREN гена 10 бактериофага Т7, терминатор транскрипции фага лямбда, ген bla β-лактамазы, определяющий устойчивость трансформированных плазмидой pTrcTREN-IL13 клеток к ампициллину, участок ori инициации репликации;ClaI / HindIII - a 4.232 kbp DNA fragment of the plasmid pTrcTEGF, including the E. coli trc promoter, synthetic translation enhancer TREN of gene 10 of bacteriophage T7, lambda phage transcription terminator, bla β-lactamase gene, which determines the stability of transformed plasmid pTrcTREN -IL13 cells to ampicillin, ori site of replication initiation;фланкированной сайтами рестрикции ClaI и HindIII оптимизированной для бактериальной экспрессии кДНК длиной 0,347 т.п.о. с нуклеотидной последовательностью, кодирующей полипептид со свойствами интерлейкина-13 человека, представленной на фиг.2 и содержащей уникальные сайты узнавания рестрикционными эндонуклеазами, имеющие следующие координаты: NcoI - 266, EcoRI - 271, ClaI - 378, PstI - 554, HindIII - 725.flanked by restriction sites ClaI and HindIII optimized for bacterial expression of 0.347 kb cDNA with a nucleotide sequence encoding a polypeptide with the properties of human interleukin-13, shown in figure 2 and containing unique recognition sites by restriction endonucleases, having the following coordinates: NcoI - 266, EcoRI - 271, ClaI - 378, PstI - 554, HindIII - 725.2. Штамм бактерий Escherichia coli BL21(DE3)/pTrcTREN-IL13 - продуцент полипептида со свойствами интерлейкина-13 человека.2. The bacterial strain Escherichia coli BL21 (DE3) / pTrcTREN-IL13 is a producer of a polypeptide with the properties of human interleukin-13.
RU2006140996/13A2006-11-212006-11-21RECOMBINANT PLASMID DNA pTrcTREN-IL-13 ENCODING POLYPEPTIDE WITH PROPERTIES OF HUMAN INTERLEUKIN-13, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pTrcTREN-IL13 - PRODUCENT OF POLYPEPTIDE WITH PROPERTIES OF HUMAN INTERLEUKIN-13
RU2333960C1
(en)
RECOMBINANT PLASMID DNA pTrcTREN-IL-13 ENCODING POLYPEPTIDE WITH PROPERTIES OF HUMAN INTERLEUKIN-13, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pTrcTREN-IL13 - PRODUCENT OF POLYPEPTIDE WITH PROPERTIES OF HUMAN INTERLEUKIN-13
RECOMBINANT PLASMID DNA pTrcTREN-IL-13 ENCODING POLYPEPTIDE WITH PROPERTIES OF HUMAN INTERLEUKIN-13, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pTrcTREN-IL13 - PRODUCENT OF POLYPEPTIDE WITH PROPERTIES OF HUMAN INTERLEUKIN-13
RECOMBINANT PLASMID DNA pTrcTREN-IL-13 ENCODING POLYPEPTIDE WITH PROPERTIES OF HUMAN INTERLEUKIN-13, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pTrcTREN-IL13 - PRODUCENT OF POLYPEPTIDE WITH PROPERTIES OF HUMAN INTERLEUKIN-13
Recombinant plasmid DNA pTrcTREN-IL-13, which encodes a polypeptide with the properties of human interleukin-13, and the strain of Escherichia coli BL21 (DE3) / pTrcTREN-SUMMER
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