RU2002102856A - The chimeric plasmid pZZSA for the expression of the chimeric angiogenin protein and the Escherichia coli strain BL21 (DE3) pZZSA is a producer of the recombinant human angiogenin chimeric protein - Google Patents

The chimeric plasmid pZZSA for the expression of the chimeric angiogenin protein and the Escherichia coli strain BL21 (DE3) pZZSA is a producer of the recombinant human angiogenin chimeric protein

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Publication number
RU2002102856A
RU2002102856A RU2002102856/13A RU2002102856A RU2002102856A RU 2002102856 A RU2002102856 A RU 2002102856A RU 2002102856/13 A RU2002102856/13 A RU 2002102856/13A RU 2002102856 A RU2002102856 A RU 2002102856A RU 2002102856 A RU2002102856 A RU 2002102856A
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RU
Russia
Prior art keywords
chimeric
pzzsa
angiogenin
protein
plasmid
Prior art date
Application number
RU2002102856/13A
Other languages
Russian (ru)
Other versions
RU2221043C2 (en
Inventor
Николай Павлович Мертвецов
Юрий Ахметович Рамазанов
Валентина Федоровна Майстренко
Original Assignee
Николай Павлович Мертвецов
Юрий Ахметович Рамазанов
Filing date
Publication date
Application filed by Николай Павлович Мертвецов, Юрий Ахметович Рамазанов filed Critical Николай Павлович Мертвецов
Priority to RU2002102856/13A priority Critical patent/RU2221043C2/en
Priority claimed from RU2002102856/13A external-priority patent/RU2221043C2/en
Priority to US10/502,554 priority patent/US20050148061A1/en
Priority to PCT/RU2003/000049 priority patent/WO2003064660A1/en
Publication of RU2002102856A publication Critical patent/RU2002102856A/en
Application granted granted Critical
Publication of RU2221043C2 publication Critical patent/RU2221043C2/en

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Claims (2)

1. Гибридная плазмида pZZSA, кодирующая синтез химерного белка ангиогенина с молекулярной массой 3,814 мегадальтон (Md) (6192 п.о.), содержащая XhoI/EcoRI - фрагмента ДНК плазмиды pGM280 (3720 п.о.), включающий тандем промоторов триптофанового оперона Е. coli, терминатор транскрипции фага лямбда, ген bla - лактамазы и участок ori инициации репликации, EcoRI/ EcoNI - фрагмента плазмиды PfM (2500 п.о.), включающий синтетический усилитель трансляции гена бактериофага Т7, и ген Ар, кодирующий аминокислотную последовательность, соответствующую 3988-4845п.о., тандем промоторов триптофанового оперона E.coli, синтетического химерного гена ангиогенина (Ang), слитого с Spa, генетического маркера - гена bla бета-лактамазы, детерминирующий устойчивость трансформированных плазмидой pZZSA клеток E.coli к ампициллину, уникальных сайтов узнавания рестрикционными эндонуклеазами, расположенными на следующих расстояниях вправо от сайта EcoRI (192 п.о.) со следующими координатами: EcoRI - 192 п.о., XbaI - 276 п.о., Bgl II - 342 п.о., Sph I - 539 п.о., EcoNI -599 п.о., Mlu 1-1064 п.о.1. The hybrid plasmid pZZSA, encoding the synthesis of a chimeric angiogenin protein with a molecular weight of 3.814 megadaltons (Md) (6192 bp), containing XhoI / EcoRI - DNA fragment of the plasmid pGM280 (3720 bp), including the tandem of tryptophan operon promoters coli, lambda phage transcription terminator, bla lactamase gene and replication initiation ori site, EcoRI / EcoNI fragment of the PfM plasmid (2500 bp), including a synthetic translation enhancer of the T7 bacteriophage gene, and the Ap gene encoding the amino acid sequence corresponding to 3988-4845p.p., tandem of tryptophan promoters of the operon of E. coli, a synthetic chimeric gene of angiogenin (Ang), fused to Spa, a genetic marker - the bla beta-lactamase gene, which determines the resistance of ampicillin to E. coli transformed by the pZZSA plasmid, unique recognition sites by restriction endonucleases located at the following distances to the right from the EcoRI website (192 bp) with the following coordinates: EcoRI - 192 bp, XbaI - 276 bp, Bgl II - 342 bp, Sph I - 539 bp, EcoNI - 599 bp, Mlu 1-1064 bp 2. Штамм Escherichia coli BL21(DE3) pZZSA (Коллекция микроорганизмов Межрегионального центра коррекции микроценоза человека, коллекционный номер МЦКМ В-127) - суперпродуцент рекомбинантного химерного белка - ангиогенина человека.2. The strain Escherichia coli BL21 (DE3) pZZSA (Collection of microorganisms of the Interregional center for the correction of human microcenosis, collection number MCCM B-127) is a superproducer of the recombinant chimeric protein - human angiogenin.
RU2002102856/13A 2002-01-31 2002-01-31 Chimera plasmid pzzsa for expression of chimera protein angiogen and strain of escherichia coli bl21(de3) pzzsa as producer of recombinant chimera human protein angiogen RU2221043C2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
RU2002102856/13A RU2221043C2 (en) 2002-01-31 2002-01-31 Chimera plasmid pzzsa for expression of chimera protein angiogen and strain of escherichia coli bl21(de3) pzzsa as producer of recombinant chimera human protein angiogen
US10/502,554 US20050148061A1 (en) 2002-01-31 2003-01-30 Hybrid plasmid pzzsa coding the synthesis of angiogenis of angiogenin protein and escherichia coli bl21(des) pzzsa strain as the the superoducer of the recombinant chimeric protein of human angiogenin
PCT/RU2003/000049 WO2003064660A1 (en) 2002-01-31 2003-01-30 Hybrid plasmid pzzsa coding the synthesis of angiogenin protein and escherichia coli bl21 (des) pzzsa strain as the superproducer of the recombinant chimeric protein of human angiogenin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2002102856/13A RU2221043C2 (en) 2002-01-31 2002-01-31 Chimera plasmid pzzsa for expression of chimera protein angiogen and strain of escherichia coli bl21(de3) pzzsa as producer of recombinant chimera human protein angiogen

Publications (2)

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RU2002102856A true RU2002102856A (en) 2003-09-10
RU2221043C2 RU2221043C2 (en) 2004-01-10

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RU2002102856/13A RU2221043C2 (en) 2002-01-31 2002-01-31 Chimera plasmid pzzsa for expression of chimera protein angiogen and strain of escherichia coli bl21(de3) pzzsa as producer of recombinant chimera human protein angiogen

Country Status (3)

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US (1) US20050148061A1 (en)
RU (1) RU2221043C2 (en)
WO (1) WO2003064660A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013511261A (en) * 2009-11-18 2013-04-04 マリー ゴールバーン シーオー−オペレイティブ シーオー．リミテッド Recombinant microorganism
RU2512527C2 (en) * 2012-07-10 2014-04-10 Общество С Ограниченной Ответственностью "Центр Вихревых Технологий" ARTIFICIAL GENE, CODING CIMERIC PROTEIN OF HUMAN ANGIOGENIN, CHIMERIC PLASMID pJZZ-A, ENSURING EXPRESSION OF GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN IN Escherichia coli AND STRAIN OF Escherichia coli BL21(DE3)/pJZZ-A-PRODUCENT OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN
RU2658758C1 (en) * 2017-10-02 2018-06-22 ООО "Лаборатория Ангиофарм" Recombinant plasmid for expression in pichia pastoris yeast gene of chimeric protein of human angiogenin and strain of pichia pastoris yeast - producer of recombinant chimeric protein of human angiogenin

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4229531A (en) * 1978-12-29 1980-10-21 Monsanto Company Production of tumor angiogenesis factor by cell culture
US5643757A (en) * 1994-03-21 1997-07-01 American Cyanamid Company High yield production of human apolipoprotein A1 in E. coli.

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