RU2010134595A - Recombinant plasmid DNA pET22b (+) / SLARP-1, encoding the protein SLARP-1, and the strain of bacteria Escherichia coli BL21 (DE3) / pET22b (+) / SLARP-1 - PRODUCT OF PROTEIN PROTEIN - Google Patents
Recombinant plasmid DNA pET22b (+) / SLARP-1, encoding the protein SLARP-1, and the strain of bacteria Escherichia coli BL21 (DE3) / pET22b (+) / SLARP-1 - PRODUCT OF PROTEIN PROTEIN Download PDFInfo
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- RU2010134595A RU2010134595A RU2010134595/10A RU2010134595A RU2010134595A RU 2010134595 A RU2010134595 A RU 2010134595A RU 2010134595/10 A RU2010134595/10 A RU 2010134595/10A RU 2010134595 A RU2010134595 A RU 2010134595A RU 2010134595 A RU2010134595 A RU 2010134595A
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Abstract
1. Рекомбинантная плазмидная ДНК pET22b(+)/SLURP-1, кодирующая белок SLURP-1 человека 5660 п.о., состоящая из NdeI/BamHI - фрагмента ДНК плазмиды рЕТ22b(+) длиной 5403 п.о., содержащего lac-промотор Е.соli, ген bla β-лактамазы, фрагмент гена lacI Е.coli, участок ori инициации репликации и искусственную последовательность ДНК, кодирующую NdeI/BamHI фрагмент длиной 257 п.о., включающий синтетический ген SLURP-1 человека в виде SEQ ID NO:7; содержащая уникальные сайты узнавания рестрикционными эндонуклеазами, имеющие следующие координаты: XhoI - 158, HindIII - 173, EcoRI - 192, BamHI - 198, NdeI - 430, BglII - 534, MluI - 1256, PstI - 4495. ! 2. Штамм бактерий Escherichia coli BL21(DE3)/pET22b(+)/SLURP-1 - продуцент белка SLURP-1 человека, полученный путем трансформации компетентных клеток Escherichia coli BL21(DE3) рекомбинантной плазмидной ДНК pET22b(+)/SLURP-1. 1. Recombinant plasmid DNA pET22b (+) / SLURP-1 encoding a 5660 bp human SLURP-1 protein, consisting of NdeI / BamHI, a pET22b (+) plasmid DNA fragment of 5403 bp in length containing the lac promoter E. coli, β-lactamase bla gene, E. coli lacI gene fragment, replication initiation ori region and artificial DNA sequence encoding a 257 bp NdeI / BamHI fragment including the synthetic human SLURP-1 gene as SEQ ID NO : 7; containing unique recognition sites by restriction endonucleases having the following coordinates: XhoI - 158, HindIII - 173, EcoRI - 192, BamHI - 198, NdeI - 430, BglII - 534, MluI - 1256, PstI - 4495.! 2. The bacterial strain Escherichia coli BL21 (DE3) / pET22b (+) / SLURP-1 is a producer of human SLURP-1 protein obtained by transformation of competent Escherichia coli BL21 (DE3) cells with recombinant plasmid DNA pET22b (+) / SLURP-1.
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| Application Number | Priority Date | Filing Date | Title |
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| RU2010134595/10A RU2453602C2 (en) | 2010-08-19 | 2010-08-19 | RECOMBINANT PLASMID DNA pET22b(+)/SLURP-1 CODING SLURP-1 PROTEIN, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pET22b(+)/SLURP-L-PRODUCENT OF HUMAN SLURP-1 PROTEIN |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2010134595/10A RU2453602C2 (en) | 2010-08-19 | 2010-08-19 | RECOMBINANT PLASMID DNA pET22b(+)/SLURP-1 CODING SLURP-1 PROTEIN, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pET22b(+)/SLURP-L-PRODUCENT OF HUMAN SLURP-1 PROTEIN |
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| RU2010134595A true RU2010134595A (en) | 2012-02-27 |
| RU2453602C2 RU2453602C2 (en) | 2012-06-20 |
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| RU2010134595/10A RU2453602C2 (en) | 2010-08-19 | 2010-08-19 | RECOMBINANT PLASMID DNA pET22b(+)/SLURP-1 CODING SLURP-1 PROTEIN, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pET22b(+)/SLURP-L-PRODUCENT OF HUMAN SLURP-1 PROTEIN |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2583307C2 (en) * | 2015-03-16 | 2016-05-10 | Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) | RECOMBINANT PLASMID DNA pET22b(+)/slurp-2 CODING SLURP-2 PROTEIN, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3) pET22b(+)/slurp-2 PRODUCER OF HUMAN SLURP-2 PROTEIN |
| RU2634385C1 (en) * | 2016-09-30 | 2017-10-26 | Федеральное бюджетное учреждение науки "Государственный научный центр прикладной микробиологии и биотехнологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ГНЦ ПМБ) | Method for obtaining of recombinant peptidoglycan-associated lipoprotein (pal) legionella pneumophila |
| RU2751132C1 (en) * | 2020-05-29 | 2021-07-08 | Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) | Application of recombinant analogue of human protein slurp-1 in combination with cytostatics or proteasome inhibitor products for inhibiting growth of carcinomas |
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| EP1514926A4 (en) * | 2002-05-31 | 2006-01-11 | Hidetoshi Inoko | Inflammatory skin disease-associated slurp-2 gene, and uses thereof |
| ES2359408T3 (en) * | 2003-04-16 | 2011-05-23 | Merck Serono Sa | USE OF SLURP-1 TO TREAT DISEASES RELATED TO THE DYSFUNCTION OF ACETILCOLINE RECEPTORS. |
| WO2007069249A2 (en) * | 2005-12-12 | 2007-06-21 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Toxin-like polypeptides, polynucleotides encoding same and uses thereof |
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| RU2453602C2 (en) | 2012-06-20 |
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Effective date: 20180921 |