RU97114729A - Recombinant Plasmid DNA PGSDEI Encoding Protein E of Tick-Encephalitis Virus and ESCHERICHIA COLI Strain - Producer of Recombinant Protein E of Tick-Encephalitis Virus - Google Patents
Recombinant Plasmid DNA PGSDEI Encoding Protein E of Tick-Encephalitis Virus and ESCHERICHIA COLI Strain - Producer of Recombinant Protein E of Tick-Encephalitis VirusInfo
- Publication number
- RU97114729A RU97114729A RU97114729/13A RU97114729A RU97114729A RU 97114729 A RU97114729 A RU 97114729A RU 97114729/13 A RU97114729/13 A RU 97114729/13A RU 97114729 A RU97114729 A RU 97114729A RU 97114729 A RU97114729 A RU 97114729A
- Authority
- RU
- Russia
- Prior art keywords
- tick
- bglii
- encephalitis virus
- site
- gene
- Prior art date
Links
- 241000588724 Escherichia coli Species 0.000 title claims 3
- 108060004132 ispG Proteins 0.000 title claims 3
- 229920003013 deoxyribonucleic acid Polymers 0.000 title claims 2
- 241000700605 Viruses Species 0.000 title 2
- 241000710771 Tick-borne encephalitis virus Species 0.000 claims 5
- 108020004705 Codon Proteins 0.000 claims 4
- 238000003776 cleavage reaction Methods 0.000 claims 3
- 108091007521 restriction endonucleases Proteins 0.000 claims 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N Ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 claims 2
- 229960000723 ampicillin Drugs 0.000 claims 2
- 230000001580 bacterial Effects 0.000 claims 2
- QIVBCDIJIAJPQS-SECBINFHSA-N D-tryptophane Chemical compound C1=CC=C2C(C[C@@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-SECBINFHSA-N 0.000 claims 1
- 210000004898 N-terminal fragment Anatomy 0.000 claims 1
- 102000007312 Recombinant Proteins Human genes 0.000 claims 1
- 108010033725 Recombinant Proteins Proteins 0.000 claims 1
- 108060007275 SCAI Proteins 0.000 claims 1
- 102000004965 antibodies Human genes 0.000 claims 1
- 108090001123 antibodies Proteins 0.000 claims 1
- 108010003152 bacteriophage T7 RNA polymerase Proteins 0.000 claims 1
- 230000002068 genetic Effects 0.000 claims 1
- 230000002209 hydrophobic Effects 0.000 claims 1
- 230000000977 initiatory Effects 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
Claims (2)
Hindlll-Scal - фрагмент плазмиды pEZZT318 размером 1165 п.о., содержащий р-независимый терминатор транскрипции триптофанового оперона E.coli(trpT) вместе с N-концевым фрагментом гена устойчивости к ампициллину(bla);
ScaI-SacI - фрагмент плазмиды pGEMI, размером 1640 п.о., включающий Т7-промотор, С-концевую часть гена bla, а также точку начала репликации;
SacI-BglII - синтетический фрагмент длиной 12 п.о., содержащий сайт связывания с рибосомами;
BglII-HindIII - фрагмент, полученный методом ПЦР, размером 1250 п.о., содержащий стартующий ATG-кодон, кодирующую часть гена Е ВКЭ без гидрофобного якоря, а также терминирующий кодон ТАА;
один участок расщепления рестриктазы BglII;
один участок расщепления рестриктазы HindIII, расположенный на расстоянии 1250 п.о. от сайта BglII по часовой стрелке;
один участок расщепления рестриктазы ScaI, расположенный на расстоянии 2897 п.о. от сайта BglII по часовой стрелке;
гентический маркер - bla-ген, обеспечивающий устойчивость к ампициллину, расположенный в участке от 1027 до 1887 п.о. вправо от BglII сайта;
промотор Т7 РНК-полимеразы, расположенный на 16 п.о. левее BglII-сайта (позиции 4055 - 4039 п.о.);
ген Е ВКЭ, расположенный на участке от 9 до 1259 п.о. по часовой стрелке от BglII сайта и имеющий собственный бактериальный участок связывания с рибосомами и инициирующий ATG-кодон, а также стоп-кодон ТАА.1. Recombinant plasmid DNA pGSDEl encoding protein E of tick-borne encephalitis virus with a molecular weight of 2.4 MD (4067 bp), containing
Hindlll-Scal - fragment of plasmid pEZZT318 with a size of 1165 bp containing a p-independent terminator for transcription of the tryptophan operon E. coli (trpT) together with the N-terminal fragment of the gene for resistance to ampicillin (bla);
ScaI-SacI - a fragment of plasmid pGEMI, 1640 bp in size, including the T7 promoter, the C-terminal portion of the bla gene, as well as the origin of replication;
SacI-BglII is a 12 bp synthetic fragment containing a ribosome binding site;
BglII-HindIII - fragment obtained by PCR, size 1250 bp, containing the start ATG codon, the coding part of the TBEV gene E without a hydrophobic anchor, as well as the TAA termination codon;
one BglII restriction enzyme cleavage site;
one HindIII restriction enzyme cleavage site located at a distance of 1250 bp from the BglII site clockwise;
one ScaI restriction enzyme cleavage site located at a distance of 2897 bp from the BglII site clockwise;
the genetic marker is the bla gene, which provides ampicillin resistance, located in the region from 1027 to 1887 bp to the right of the BglII site;
T7 RNA polymerase promoter located at 16 bp to the left of the BglII site (positions 4055 - 4039 bp);
TBEV E gene located in the region from 9 to 1259 bp clockwise from the BglII site and having its own bacterial ribosome binding site and the initiating ATG codon, as well as the TAA stop codon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU97114729A RU2136754C1 (en) | 1997-09-02 | 1997-09-02 | Recombinant dna plasmid pgsde1 encoding tick-borne encephalitis virus protein e and strain of escherichia coli - producer of tick-borne encephalitis virus protein e |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU97114729A RU2136754C1 (en) | 1997-09-02 | 1997-09-02 | Recombinant dna plasmid pgsde1 encoding tick-borne encephalitis virus protein e and strain of escherichia coli - producer of tick-borne encephalitis virus protein e |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU97114729A true RU97114729A (en) | 1999-07-20 |
| RU2136754C1 RU2136754C1 (en) | 1999-09-10 |
Family
ID=20196835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU97114729A RU2136754C1 (en) | 1997-09-02 | 1997-09-02 | Recombinant dna plasmid pgsde1 encoding tick-borne encephalitis virus protein e and strain of escherichia coli - producer of tick-borne encephalitis virus protein e |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2136754C1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2562841C2 (en) * | 2013-12-30 | 2015-09-10 | ЗАО "МБС-Технология" | Method of preparing tick tissues for biological analysis |
-
1997
- 1997-09-02 RU RU97114729A patent/RU2136754C1/en not_active IP Right Cessation
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