RU97107950A - POLYPEPTIDE E 495 for determining antibodies to T-cell leukemia I TYPE (HTLV-I), DNA fragments ATLE 495 encoding the polypeptide E 495, with recombinant plasmid DNA PATLE 495 encoding the polypeptide E 495, STRAIN BACTERIA ESCHERICHIA COLI - PRODUCER POLYPEPTIDE E 495. - Google Patents

POLYPEPTIDE E 495 for determining antibodies to T-cell leukemia I TYPE (HTLV-I), DNA fragments ATLE 495 encoding the polypeptide E 495, with recombinant plasmid DNA PATLE 495 encoding the polypeptide E 495, STRAIN BACTERIA ESCHERICHIA COLI - PRODUCER POLYPEPTIDE E 495.

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Publication number
RU97107950A
RU97107950A RU97107950/13A RU97107950A RU97107950A RU 97107950 A RU97107950 A RU 97107950A RU 97107950/13 A RU97107950/13 A RU 97107950/13A RU 97107950 A RU97107950 A RU 97107950A RU 97107950 A RU97107950 A RU 97107950A
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RU
Russia
Prior art keywords
polypeptide
encoding
htlv
patle
atle
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RU97107950/13A
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Russian (ru)
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RU2152955C2 (en
Inventor
А.Ф. Бобков
М.Н. Санков
Д.М. Селимова
Е.В. Казеннова
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Научно-исследовательский институт вирусологии
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Priority to RU97107950A priority Critical patent/RU2152955C2/en
Priority claimed from RU97107950A external-priority patent/RU2152955C2/en
Publication of RU97107950A publication Critical patent/RU97107950A/en
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Claims (4)

1. Полипептид Е 495, предназначенный для определения антител к вирусу Т-клеточного лейкоза человека I типа (HTLV-I), полученный в штамме бактерий Escherichia coli НВ101, трансформированном рекомбинантной плазмидой pATLE 495, кодирующей полипептид с аминокислотной последовательностью, приведенной в описании, слитой с бета-галактозидазой E.coli и обладающей способностью связываться с антителами к продукту гена env HTLV-1, с молекулярной массой 133 кило-дальтон при определении методом электрофореза в полиакриламидном геле.1. The polypeptide E 495, designed to detect antibodies to the human T-cell leukemia virus type I (HTLV-I), obtained in the bacterial strain Escherichia coli HB101, transformed with recombinant plasmid pATLE 495, encoding a polypeptide with the amino acid sequence described in the description, fused with E.coli beta-galactosidase and capable of binding to antibodies to the env HTLV-1 gene product, with a molecular weight of 133 kilo-daltons as determined by polyacrylamide gel electrophoresis. 2. Фрагмент ДНК HTLV-I (ATLE 495), кодирующий полипептид Е 495, полученный с помощью цепной полимеразной реакции, с нуклеотидной последовательностью, приведенной в описании, содержащий на 3' конце стоп-кодон полилинкера pUR291. 2. The HTLV-I DNA fragment (ATLE 495) encoding the E 495 polypeptide obtained by the polymerase chain reaction with the nucleotide sequence described in the description containing at the 3 'end a stop codon of the polylinker pUR291. 3. Рекомбинантная плазмидная ДНК pATLE 495, кодирующая полипептид Е 495, предназначенный для определения антител к вирусу Т-лейкоза человека I типа, размером 5,6 т.п.н., содержащая SalGI - фрагмент ДНК вектора pUR291, включающий ген бета-галактозидазы E.coli размером 5,2 т.п.н.; SalGI - BamHI - фрагмент ДНК HTLV-I (ATLE 495) размером 0,4 т.п.н., кодирующий полипептид Е 495; по одному участку расщепления рестриктазами BamHI, PstI, HindIII, StuI, ClaI, Tthlll-II; промотр LacZ оперона; генетические маркеры: ген bla (ген устойчивости к ампициллину). 3. Recombinant plasmid DNA pATLE 495, encoding the E 495 polypeptide, designed to detect antibodies to the human T-leukemia virus type I, size 5.6 TPN, containing SalGI - DNA fragment of the vector pUR291, including the beta-galactosidase gene E. coli 5.2 kb; SalGI - BamHI - 0.4 kb HTLV-I (ATLE 495) DNA fragment encoding the E 495 polypeptide; one site of restriction enzyme digestion with BamHI, PstI, HindIII, StuI, ClaI, Tthlll-II; promoter LacZ operon; genetic markers: bla gene (ampicillin resistance gene). 4. Штамм бактерий Escherichia coli HB101/pATLE 495 -продуцент полипептида Е 495, предназначенного для определения антител в вирусу Т-клеточного лейкоза человека I типа. 4. The bacterial strain Escherichia coli HB101 / pATLE 495 is a producer of the E 495 polypeptide, designed to detect antibodies in type I human T-cell leukemia virus.
RU97107950A 1997-05-15 1997-05-15 Fragment of gene env htlv-i, recombinant plasmid dna patle 495, strain of bacterium escherichia coli hb101/patle 495 as producer of polypeptide e 495 RU2152955C2 (en)

Priority Applications (1)

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RU97107950A RU2152955C2 (en) 1997-05-15 1997-05-15 Fragment of gene env htlv-i, recombinant plasmid dna patle 495, strain of bacterium escherichia coli hb101/patle 495 as producer of polypeptide e 495

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RU97107950A RU2152955C2 (en) 1997-05-15 1997-05-15 Fragment of gene env htlv-i, recombinant plasmid dna patle 495, strain of bacterium escherichia coli hb101/patle 495 as producer of polypeptide e 495

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RU97107950A true RU97107950A (en) 1999-06-10
RU2152955C2 RU2152955C2 (en) 2000-07-20

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