PT1040202E - Determinação da identidade de polimorfismos de adn utilizando citometria de fluxo - Google Patents
Determinação da identidade de polimorfismos de adn utilizando citometria de fluxo Download PDFInfo
- Publication number
- PT1040202E PT1040202E PT98955222T PT98955222T PT1040202E PT 1040202 E PT1040202 E PT 1040202E PT 98955222 T PT98955222 T PT 98955222T PT 98955222 T PT98955222 T PT 98955222T PT 1040202 E PT1040202 E PT 1040202E
- Authority
- PT
- Portugal
- Prior art keywords
- oligonucleotide
- dna
- microsphere
- base
- flow cytometry
- Prior art date
Links
- 238000000684 flow cytometry Methods 0.000 title claims abstract description 41
- 108091034117 Oligonucleotide Proteins 0.000 claims abstract description 80
- 239000004005 microsphere Substances 0.000 claims abstract description 71
- 108020004414 DNA Proteins 0.000 claims abstract description 65
- 102000053602 DNA Human genes 0.000 claims abstract description 28
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6368597P | 1997-10-28 | 1997-10-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT1040202E true PT1040202E (pt) | 2009-03-26 |
Family
ID=22050818
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT98955222T PT1040202E (pt) | 1997-10-28 | 1998-10-28 | Determinação da identidade de polimorfismos de adn utilizando citometria de fluxo |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US6287766B1 (https=) |
| EP (3) | EP1032701A1 (https=) |
| JP (3) | JP4502502B2 (https=) |
| AT (1) | ATE419382T1 (https=) |
| AU (2) | AU754849B2 (https=) |
| CA (2) | CA2308599C (https=) |
| DE (1) | DE69840416D1 (https=) |
| DK (1) | DK1040202T3 (https=) |
| ES (1) | ES2320604T3 (https=) |
| IL (2) | IL135852A0 (https=) |
| IS (2) | IS5455A (https=) |
| PT (1) | PT1040202E (https=) |
| WO (2) | WO1999022029A1 (https=) |
Families Citing this family (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4502502B2 (ja) * | 1997-10-28 | 2010-07-14 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | フローサイトメトリーを用いるｄｎａ多型の同定方法 |
| DE69830854T2 (de) * | 1997-12-04 | 2006-04-20 | Amersham Biosciences Uk Ltd., Amersham | Mehrfaches testverfahren |
| AU3519900A (en) * | 1999-03-19 | 2000-10-09 | Aclara Biosciences, Inc. | Methods for single nucleotide polymorphism detection |
| JP3668075B2 (ja) | 1999-10-12 | 2005-07-06 | 光夫 板倉 | 遺伝物質シーケンス決定用懸濁系、その懸濁系を用いた遺伝物質シーケンス決定方法およびその懸濁系を用いたＳＮＰｓ高速スコアリング方法 |
| DE19958980A1 (de) * | 1999-11-18 | 2001-09-27 | Wolfgang Goehde | Verfahren zur Bestimmung von PCR-Produkten, sowie ein PCR-Detektions-Kit und eine Vorrichtung für ein solches Verfahren |
| DK1259643T3 (da) * | 2000-02-07 | 2009-02-23 | Illumina Inc | Fremgangsmåde til detektion af nukleinsyre ved anvendelse af universel priming |
| US6365355B1 (en) | 2000-03-28 | 2002-04-02 | The Regents Of The University Of California | Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches |
| US6340566B1 (en) * | 2000-03-28 | 2002-01-22 | The Regents Of The University Of California | Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches |
| AU7125401A (en) * | 2000-05-19 | 2001-12-03 | David J Marshall | Materials and methods for detection of nucleic acids |
| KR20020065471A (ko) * | 2000-08-30 | 2002-08-13 | 산코 준야쿠 가부시키가이샤 | 유전자의 검출방법 |
| US7465540B2 (en) * | 2000-09-21 | 2008-12-16 | Luminex Corporation | Multiple reporter read-out for bioassays |
| AU1317502A (en) | 2000-10-14 | 2002-04-29 | Eragen Biosciences Inc | Solid support assay systems and methods utilizing non-standard bases |
| US6592713B2 (en) | 2000-12-18 | 2003-07-15 | Sca Hygiene Products Ab | Method of producing a nonwoven material |
| US7083951B2 (en) * | 2001-02-14 | 2006-08-01 | The Regents Of The University Of California | Flow cytometric detection method for DNA samples |
| US20020115116A1 (en) * | 2001-02-22 | 2002-08-22 | Yong Song | Multiplex protein interaction determinations using glutathione-GST binding |
| US7153656B2 (en) * | 2001-09-11 | 2006-12-26 | Los Alamos National Security, Llc | Nucleic acid sequence detection using multiplexed oligonucleotide PCR |
| CA2497740C (en) | 2001-10-15 | 2011-06-21 | Bioarray Solutions, Ltd. | Multiplexed analysis of polymorphic loci by probe elongation-mediated detection |
| US7179598B2 (en) * | 2001-11-20 | 2007-02-20 | The Regents Of The University Of California | Early leukemia diagnostics using microsphere arrays |
| AU2002357249A1 (en) * | 2001-12-13 | 2003-07-09 | Blue Heron Biotechnology, Inc. | Methods for removal of double-stranded oligonucleotides containing sequence errors using mismatch recognition proteins |
| AU2003220254B2 (en) | 2002-03-13 | 2008-09-18 | Syngenta Participations, Ag. | Nucleic acid detection method |
| US7304128B2 (en) * | 2002-06-04 | 2007-12-04 | E.I. Du Pont De Nemours And Company | Carbon nanotube binding peptides |
| CN1665938B (zh) * | 2002-06-28 | 2012-10-10 | 普里梅拉蒂斯有限公司 | 检测序列差异的方法 |
| EP1613768A4 (en) * | 2003-03-11 | 2006-05-31 | Gene Check Inc | RECA-SUPPORTED ALLELSPECIFIC OLIGONUCLEOTIDE EXTENSION PROCESS |
| EP1606419A1 (en) | 2003-03-18 | 2005-12-21 | Quantum Genetics Ireland Limited | Systems and methods for improving protein and milk production of dairy herds |
| AU2004227353B2 (en) * | 2003-04-01 | 2010-02-25 | Luminex Corporation | Polymerase inhibitor and method of using same |
| EP1613776A1 (en) * | 2003-04-02 | 2006-01-11 | Blue Heron Biotechnology, Inc. | Error reduction in automated gene synthesis |
| EP1725572B1 (de) | 2003-11-05 | 2017-05-31 | AGCT GmbH | Makromolekulare nukleotidverbindungen und methoden zu deren anwendung |
| WO2005112544A2 (en) | 2004-02-19 | 2005-12-01 | The Governors Of The University Of Alberta | Leptin promoter polymorphisms and uses thereof |
| DE102005008583B4 (de) * | 2005-02-24 | 2007-10-25 | Johannes-Gutenberg-Universität Mainz | Verfahren zur Typisierung eines Individuums mittels short tandem repeat (STR)-Loci der genomischen DNA |
| JP4399575B2 (ja) * | 2005-03-31 | 2010-01-20 | 独立行政法人産業技術総合研究所 | 遺伝子断片中のヌクレオチドの種類の特定方法 |
| US8293472B2 (en) | 2005-06-07 | 2012-10-23 | Luminex Corporation | Methods for detection and typing of nucleic acids |
| JPWO2007063807A1 (ja) * | 2005-11-29 | 2009-05-07 | オリンパス株式会社 | 核酸の一次構造変化の解析方法 |
| US7960116B2 (en) * | 2007-09-28 | 2011-06-14 | Pacific Biosciences Of California, Inc. | Nucleic acid sequencing methods and systems |
| US8871687B2 (en) * | 2007-09-28 | 2014-10-28 | Quest Diagnostics Investments Incorporated | Nucleic acid sequencing by single-base primer extension |
| US8093063B2 (en) | 2007-11-29 | 2012-01-10 | Quest Diagnostics Investments Incorporated | Assay for detecting genetic abnormalities in genomic nucleic acids |
| CN101445832B (zh) * | 2008-12-23 | 2011-09-14 | 广州益善生物技术有限公司 | Pik3ca基因突变的检测探针、液相芯片及其检测方法 |
| CN101487051B (zh) * | 2009-02-24 | 2011-07-20 | 广州益善生物技术有限公司 | Braf基因突变的检测探针、液相芯片及其检测方法 |
| CA2778449C (en) * | 2009-10-23 | 2017-06-20 | Luminex Corporation | Amplification primers with non-standard bases for increased reaction specificity |
| ITPV20100009A1 (it) * | 2010-07-15 | 2012-01-16 | Luca Lova | Rilevamento multiparametrico in sospensione per sequenze nucleotidiche |
| WO2013059746A1 (en) | 2011-10-19 | 2013-04-25 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
| EP2807292B1 (en) | 2012-01-26 | 2019-05-22 | Tecan Genomics, Inc. | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
| CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| WO2014121091A1 (en) | 2013-02-01 | 2014-08-07 | The Regents Of The University Of California | Methods for genome assembly and haplotype phasing |
| US9411930B2 (en) | 2013-02-01 | 2016-08-09 | The Regents Of The University Of California | Methods for genome assembly and haplotype phasing |
| US9540699B2 (en) | 2013-03-13 | 2017-01-10 | William Beaumont Hospital | Methods of diagnosing increased risk of developing MRSA |
| US9822408B2 (en) | 2013-03-15 | 2017-11-21 | Nugen Technologies, Inc. | Sequential sequencing |
| WO2015073711A1 (en) | 2013-11-13 | 2015-05-21 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
| EP3540074A1 (en) | 2013-12-11 | 2019-09-18 | The Regents of the University of California | Method of tagging internal regions of nucleic acid molecules |
| US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| WO2015191815A1 (en) | 2014-06-13 | 2015-12-17 | Life Technologies Corporation | Multiplex nucleic acid amplification |
| US10526641B2 (en) | 2014-08-01 | 2020-01-07 | Dovetail Genomics, Llc | Tagging nucleic acids for sequence assembly |
| CA2957633A1 (en) | 2014-08-06 | 2016-02-11 | Nugen Technologies, Inc. | Digital measurements from targeted sequencing |
| GB201501603D0 (en) | 2015-01-30 | 2015-03-18 | Norwegian University Of Life Sciences And Hedmark University College | Method and product |
| WO2016134034A1 (en) | 2015-02-17 | 2016-08-25 | Dovetail Genomics Llc | Nucleic acid sequence assembly |
| US11807896B2 (en) | 2015-03-26 | 2023-11-07 | Dovetail Genomics, Llc | Physical linkage preservation in DNA storage |
| CA3002740A1 (en) | 2015-10-19 | 2017-04-27 | Dovetail Genomics, Llc | Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection |
| SG11201807117WA (en) | 2016-02-23 | 2018-09-27 | Dovetail Genomics Llc | Generation of phased read-sets for genome assembly and haplotype phasing |
| KR102412442B1 (ko) | 2016-05-13 | 2022-06-22 | 더브테일 제노믹스 엘엘씨 | 보존된 샘플로부터의 장범위 링키지 정보의 회수 |
| US10190155B2 (en) | 2016-10-14 | 2019-01-29 | Nugen Technologies, Inc. | Molecular tag attachment and transfer |
| DE102016124692B4 (de) | 2016-12-16 | 2019-05-16 | Gna Biosolutions Gmbh | Verfahren und System zum Vervielfältigen einer Nukleinsäure |
| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| WO2018232597A1 (zh) * | 2017-06-20 | 2018-12-27 | 深圳华大智造科技有限公司 | 用于定量pcr扩增的组合物及其应用 |
| CN109652499B (zh) * | 2017-10-12 | 2022-06-03 | 深圳华大智造科技股份有限公司 | 快速检测dna聚合酶3′-5′外切活性或错配的方法和试剂盒 |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| EP3746566A1 (en) | 2018-01-31 | 2020-12-09 | Dovetail Genomics, LLC | Sample prep for dna linkage recovery |
| US10768173B1 (en) * | 2019-09-06 | 2020-09-08 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| EP4545965A3 (en) | 2019-06-27 | 2025-06-11 | Dovetail Genomics, LLC | Methods and compositions for proximity ligation |
| US11287422B2 (en) | 2019-09-23 | 2022-03-29 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| EP3851542A1 (en) | 2020-01-20 | 2021-07-21 | Tecan Genomics, Inc. | Depletion of abundant uninformative sequences |
| US12059674B2 (en) | 2020-02-03 | 2024-08-13 | Tecan Genomics, Inc. | Reagent storage system |
| EP4573213A1 (en) | 2022-08-15 | 2025-06-25 | Element Biosciences, Inc. | Spatially resolved surface capture of nucleic acids |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4770992A (en) * | 1985-11-27 | 1988-09-13 | Den Engh Gerrit J Van | Detection of specific DNA sequences by flow cytometry |
| US4962037A (en) * | 1987-10-07 | 1990-10-09 | United States Of America | Method for rapid base sequencing in DNA and RNA |
| US4988617A (en) * | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
| US5512439A (en) * | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
| US5629158A (en) * | 1989-03-22 | 1997-05-13 | Cemu Bitecknik Ab | Solid phase diagnosis of medical conditions |
| US5437976A (en) * | 1991-08-08 | 1995-08-01 | Arizona Board Of Regents, The University Of Arizona | Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA |
| AU3919293A (en) * | 1992-03-27 | 1993-11-08 | University Of Maryland At Baltimore | Detection of gene mutations with mismatch repair enzymes |
| EP0705905B1 (de) * | 1994-07-16 | 2001-10-10 | Roche Diagnostics GmbH | Verfahren zum sensitiven Nachweis von Nukleinsäuren |
| US5681702A (en) * | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| WO1996015271A1 (en) * | 1994-11-16 | 1996-05-23 | Abbott Laboratories | Multiplex ligations-dependent amplification |
| US5736330A (en) * | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
| WO1997014028A2 (en) * | 1995-10-11 | 1997-04-17 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| US5670325A (en) * | 1996-08-14 | 1997-09-23 | Exact Laboratories, Inc. | Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample |
| ES2220997T3 (es) * | 1995-12-22 | 2004-12-16 | Exact Sciences Corporation | Metodos para la deteccion de poblaciones clonales de celulas transformadas en una muestra celular genomicamente heterogenea. |
| JP4540754B2 (ja) * | 1996-06-04 | 2010-09-08 | ユニバーシティ オブ ユタ リサーチ ファウンデーション | Ｐｃｒ中のハイブリダイゼーションのモニタリング |
| JP2002508664A (ja) * | 1997-06-25 | 2002-03-19 | オーキッド・バイオサイエンシーズ・インコーポレイテッド | 複数の単一ヌクレオチド多型を単一の反応で検出する方法 |
| JP4502502B2 (ja) * | 1997-10-28 | 2010-07-14 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | フローサイトメトリーを用いるｄｎａ多型の同定方法 |
| EP1090138A4 (en) | 1998-06-24 | 2003-01-02 | Glaxo Group Ltd | DETECTION METHOD FOR NUCLEOTIDES |
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1998
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- 1998-10-28 AT AT98955222T patent/ATE419382T1/de active
- 1998-10-28 PT PT98955222T patent/PT1040202E/pt unknown
- 1998-10-28 US US09/182,869 patent/US6287766B1/en not_active Expired - Lifetime
- 1998-10-28 IL IL13585298A patent/IL135852A0/xx unknown
- 1998-10-28 EP EP98955221A patent/EP1032701A1/en not_active Withdrawn
- 1998-10-28 WO PCT/US1998/023142 patent/WO1999022029A1/en not_active Ceased
- 1998-10-28 AU AU12078/99A patent/AU754849B2/en not_active Ceased
- 1998-10-28 IL IL13585198A patent/IL135851A/en not_active IP Right Cessation
- 1998-10-28 CA CA2308599A patent/CA2308599C/en not_active Expired - Fee Related
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- 1998-10-28 ES ES98955222T patent/ES2320604T3/es not_active Expired - Lifetime
- 1998-10-28 DK DK98955222T patent/DK1040202T3/da active
- 1998-10-28 WO PCT/US1998/023143 patent/WO1999022030A1/en not_active Ceased
- 1998-10-28 EP EP98955222A patent/EP1040202B9/en not_active Expired - Lifetime
- 1998-10-28 AU AU12077/99A patent/AU1207799A/en not_active Abandoned
- 1998-10-28 CA CA002306907A patent/CA2306907A1/en not_active Abandoned
- 1998-10-28 DE DE69840416T patent/DE69840416D1/de not_active Expired - Lifetime
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2000
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| US6287766B1 (en) | 2001-09-11 |
| CA2308599C (en) | 2012-05-22 |
| EP1040202A1 (en) | 2000-10-04 |
| IS2699B (is) | 2010-11-15 |
| EP1040202B9 (en) | 2009-09-23 |
| WO1999022029A1 (en) | 1999-05-06 |
| EP1040202B1 (en) | 2008-12-31 |
| IL135851A0 (en) | 2001-05-20 |
| EP1040202A4 (en) | 2004-08-18 |
| JP4502502B2 (ja) | 2010-07-14 |
| ES2320604T3 (es) | 2009-05-25 |
| IS5454A (is) | 2000-04-14 |
| IL135851A (en) | 2004-03-28 |
| IS5455A (is) | 2000-04-14 |
| JP2001520895A (ja) | 2001-11-06 |
| EP1032701A1 (en) | 2000-09-06 |
| AU754849B2 (en) | 2002-11-28 |
| AU1207799A (en) | 1999-05-17 |
| US20020015962A1 (en) | 2002-02-07 |
| IL135852A0 (en) | 2001-05-20 |
| EP2034031A1 (en) | 2009-03-11 |
| CA2306907A1 (en) | 1999-05-06 |
| JP2010088454A (ja) | 2010-04-22 |
| CA2308599A1 (en) | 1999-05-06 |
| JP2003502005A (ja) | 2003-01-21 |
| AU1207899A (en) | 1999-05-17 |
| DK1040202T3 (da) | 2009-04-14 |
| ATE419382T1 (de) | 2009-01-15 |
| WO1999022030A1 (en) | 1999-05-06 |
| DE69840416D1 (de) | 2009-02-12 |
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