PL3746564T3 - Sposób amplifikacji kwasów nukleinowych - Google Patents

Sposób amplifikacji kwasów nukleinowych

Info

Publication number
PL3746564T3
PL3746564T3 PL19743724.7T PL19743724T PL3746564T3 PL 3746564 T3 PL3746564 T3 PL 3746564T3 PL 19743724 T PL19743724 T PL 19743724T PL 3746564 T3 PL3746564 T3 PL 3746564T3
Authority
PL
Poland
Prior art keywords
amplification
nucleic acids
nucleic
acids
Prior art date
Application number
PL19743724.7T
Other languages
English (en)
Inventor
Charles GAWAD
John Easton
Veronica GONZALEZ-PENA
Original Assignee
St. Jude Children's Research Hospital, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by St. Jude Children's Research Hospital, Inc. filed Critical St. Jude Children's Research Hospital, Inc.
Publication of PL3746564T3 publication Critical patent/PL3746564T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/319—Exonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
PL19743724.7T 2018-01-29 2019-01-28 Sposób amplifikacji kwasów nukleinowych PL3746564T3 (pl)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862623471P 2018-01-29 2018-01-29
PCT/US2019/015452 WO2019148119A1 (en) 2018-01-29 2019-01-28 Method for nucleic acid amplification

Publications (1)

Publication Number Publication Date
PL3746564T3 true PL3746564T3 (pl) 2023-05-22

Family

ID=67396233

Family Applications (2)

Application Number Title Priority Date Filing Date
PL19743724.7T PL3746564T3 (pl) 2018-01-29 2019-01-28 Sposób amplifikacji kwasów nukleinowych
PL22217020.1T PL4183886T3 (pl) 2018-01-29 2019-01-28 Sposób amplifikacji kwasów nukleinowych

Family Applications After (1)

Application Number Title Priority Date Filing Date
PL22217020.1T PL4183886T3 (pl) 2018-01-29 2019-01-28 Sposób amplifikacji kwasów nukleinowych

Country Status (14)

Country Link
US (4) US11905553B2 (pl)
EP (2) EP3746564B1 (pl)
JP (1) JP7457434B2 (pl)
KR (1) KR102653725B1 (pl)
CN (1) CN112041459B (pl)
AU (3) AU2019212953B2 (pl)
CA (1) CA3089756A1 (pl)
DK (2) DK3746564T3 (pl)
EA (1) EA202091673A1 (pl)
ES (2) ES2983417T3 (pl)
IL (2) IL312691A (pl)
PL (2) PL3746564T3 (pl)
SG (1) SG11202007225TA (pl)
WO (1) WO2019148119A1 (pl)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2983417T3 (es) 2018-01-29 2024-10-23 St Jude Childrens Res Hospital Inc Método para la amplificación de ácidos nucleicos
US12226746B2 (en) 2019-06-07 2025-02-18 Microsoft Technology Licensing, Llc Reversing bias in polymer synthesis electrode array
WO2021022085A2 (en) * 2019-07-31 2021-02-04 Bioskryb, Inc. Single cell analysis
CN114466935B (zh) * 2019-07-31 2025-01-17 铂赛基因组学公司 基因突变分析
US11773422B2 (en) 2019-08-16 2023-10-03 Microsoft Technology Licensing, Llc Regulation of polymerase using cofactor oxidation states
US11795450B2 (en) * 2019-09-06 2023-10-24 Microsoft Technology Licensing, Llc Array-based enzymatic oligonucleotide synthesis
CN112831543B (zh) * 2019-11-25 2023-08-29 中国科学院青岛生物能源与过程研究所 单细胞全基因组扩增试剂盒及其应用
AU2021219665A1 (en) * 2020-02-10 2022-09-01 BioSkryb Genomics, Inc. Phi29 mutants and use thereof
CA3145539A1 (en) * 2020-02-26 2021-09-02 Illumina, Inc. Kits for genotyping
EP4146815A1 (en) * 2020-05-07 2023-03-15 Bioskryb Genomics, Inc. Detection of low abundance nucleic acids
US11702683B2 (en) 2020-05-28 2023-07-18 Microsoft Technology Licensing, Llc De novo polynucleotide synthesis with substrate-bound polymerase
US12371743B2 (en) 2022-03-04 2025-07-29 Element Biosciences, Inc. Double-stranded splint adaptors and methods of use
US12359193B2 (en) 2022-03-04 2025-07-15 Element Biosciences, Inc. Single-stranded splint strands and methods of use
US12344876B2 (en) 2020-10-30 2025-07-01 Microsoft Technology Licensing, Llc Spatially addressable control of polymerase activity
WO2023277953A1 (en) * 2021-06-29 2023-01-05 Agilent Technologies, Inc. Polymerase mutants and use with 3'-oh unblocked reversible terminators
GB202111194D0 (en) 2021-08-03 2021-09-15 Cergentis B V Method
WO2023107453A1 (en) * 2021-12-06 2023-06-15 Chan Zuckerberg Biohub, Inc. Method for combined genome methylation and variation analyses
CN119585443A (zh) * 2022-05-05 2025-03-07 铂赛基因组学公司 原代模板定向扩增及其方法
KR20240031934A (ko) * 2022-09-01 2024-03-08 주식회사 키오믹스 다중 표적 dna의 선택적 증폭용 조성물 및 이를 이용한 증폭 방법
JP2025530259A (ja) 2022-09-12 2025-09-11 エレメント バイオサイエンシズ，インコーポレイティド ユニバーサル長スプリント鎖を有する二本鎖スプリントアダプター及び使用方法
CN117343999B (zh) * 2023-12-05 2024-03-01 上海锐赛循益生物技术有限公司 基于左侧探针退火和右侧探针退火延伸的核酸扩增方法
WO2025235939A1 (en) * 2024-05-10 2025-11-13 Orchid Bioscience, Inc. Methods for balanced amplification of small samples of nucleic acids

Family Cites Families (209)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
FR2649518B1 (fr) 1989-07-07 1991-10-18 Bioprobe Systems Sa Procede et dispositif de marquage crypte de haute securite pour la protection d'objets de valeur
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
AU7398996A (en) 1995-10-11 1997-04-30 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6432360B1 (en) 1997-10-10 2002-08-13 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
EP1248110A3 (en) 1997-11-18 2005-01-26 Bio-Rad Laboratories, Inc. Multiplex flow immunoassays with magnetic particles as solid phase
WO1999058724A1 (en) * 1998-05-08 1999-11-18 Life Technologies, Inc. A method for synthesizing a nucleic acid molecule using a ribonuclease
US6150105A (en) 1998-08-20 2000-11-21 Genetic Assays, Inc. Methods of screening nucleic acids for nucleotide variations
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6649414B1 (en) 1999-08-17 2003-11-18 Luminex Corporation Microparticles with multiple fluorescent signals and methods of using same
ATE324588T1 (de) 1999-08-17 2006-05-15 Luminex Corp Verkapselung von fluoreszierenden partikeln
US6251639B1 (en) * 1999-09-13 2001-06-26 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer
US8529743B2 (en) 2000-07-25 2013-09-10 The Regents Of The University Of California Electrowetting-driven micropumping
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
WO2002059357A2 (en) 2001-01-24 2002-08-01 Genomic Expression Aps Assay and kit for analyzing gene expression
GB0102568D0 (en) 2001-02-01 2001-03-21 Magnetic Biosolutions Sweden A Method
WO2002086088A2 (en) 2001-04-24 2002-10-31 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
WO2003002736A2 (en) * 2001-06-27 2003-01-09 Roche Diagnostics Gmbh A walk-through technique for in vitro recombination of polynucleotide sequences
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US7297485B2 (en) 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US7655791B2 (en) 2001-11-13 2010-02-02 Rubicon Genomics, Inc. DNA amplification and sequencing using DNA molecules generated by random fragmentation
IL161988A0 (en) 2001-11-16 2005-11-20 Corporation Idec Pharmaceutica Polycistronic expression of antibodies
WO2003045556A2 (en) 2001-11-26 2003-06-05 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
US20050221324A1 (en) * 2002-05-06 2005-10-06 Fox Michael H Genotoxicity analysis
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
EP1576188B1 (en) * 2002-11-21 2008-10-15 Epicentre Technologies Methods for using riboprimers for strand displacement replication of target sequences
US7547380B2 (en) 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
ATE434131T1 (de) 2003-11-17 2009-07-15 Koninkl Philips Electronics Nv System zur handhabung einer fluidmenge
KR100695123B1 (ko) 2003-12-03 2007-03-14 삼성전자주식회사 Ｔｍ에 따라 기판상에 고정화된 프로브 폴리뉴클레오티드군을 2이상 포함하는 폴리뉴클레오티드 어레이 및 그를이용하는 표적 핵산 검출 방법
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
US7362432B2 (en) 2004-01-14 2008-04-22 Luminex Corp. Method and systems for dynamic range expansion
US20060141487A1 (en) 2004-01-26 2006-06-29 Applera Corporation Methods, compositions, and kits for amplifying and sequencing polynucleotides
FR2866493B1 (fr) 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
CN1950519A (zh) 2004-02-27 2007-04-18 哈佛大学的校长及成员们 聚合酶群落荧光原位测序珠子
US7387876B2 (en) 2004-02-27 2008-06-17 President And Fellows Of Harvard College Amplification of trace amounts of nucleic acids
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
FR2872715B1 (fr) 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
WO2006073504A2 (en) 2004-08-04 2006-07-13 President And Fellows Of Harvard College Wobble sequencing
JP2006058031A (ja) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp 化学分析装置
EP1842211B1 (en) 2005-01-20 2010-08-04 Luminex Corporation Microspheres having fluorescent and magnetic properties
US7458661B2 (en) 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
DK1859330T3 (da) 2005-01-28 2012-10-15 Univ Duke Apparater og fremgangsmåder til håndtering af små dråber på et trykt kredsløbskort
US9040237B2 (en) * 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US20070023292A1 (en) 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
US7544471B2 (en) 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
US20070031857A1 (en) 2005-08-02 2007-02-08 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction
EP1929046B1 (en) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
EP2261635B1 (en) 2005-09-21 2017-05-03 Luminex Corporation Methods and systems for image data processing
EP2458011B1 (en) * 2005-10-03 2013-07-24 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
WO2007120208A2 (en) 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
AU2006330947B2 (en) 2005-12-22 2012-04-12 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
US20070207513A1 (en) 2006-03-03 2007-09-06 Luminex Corporation Methods, Products, and Kits for Identifying an Analyte in a Sample
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
CA2680532C (en) 2006-04-18 2017-03-21 Advanced Liquid Logic, Inc. Droplet-based pyrosequencing
JP4881950B2 (ja) 2006-07-10 2012-02-22 株式会社日立ハイテクノロジーズ 液体搬送デバイス
WO2008055256A2 (en) 2006-11-02 2008-05-08 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
AU2007328427A1 (en) 2006-11-06 2008-06-12 Source Precision Medicine, Inc. Gene expression profiling for identification, monitoring and treatment of melanoma
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US7897737B2 (en) * 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US7893227B2 (en) * 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
KR101433208B1 (ko) 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 ｐｃｒ의 다중 분석을 위한 시스템과 방법
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
KR101503510B1 (ko) 2007-02-09 2015-03-18 어드밴스드 리퀴드 로직, 아이엔씨. 자성 비즈를 이용하는 액적 작동기 장치 및 방법
WO2008116221A1 (en) 2007-03-22 2008-09-25 Advanced Liquid Logic, Inc. Bead sorting on a droplet actuator
WO2008134153A1 (en) 2007-04-23 2008-11-06 Advanced Liquid Logic, Inc. Bead-based multiplexed analytical methods and instrumentation
US8926811B2 (en) 2007-06-27 2015-01-06 Digital Biosystems Digital microfluidics based apparatus for heat-exchanging chemical processes
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US8685642B2 (en) 2007-07-30 2014-04-01 Agilent Technologies, Inc. Allele-specific copy number measurement using single nucleotide polymorphism and DNA arrays
US9309511B2 (en) 2007-08-28 2016-04-12 The Johns Hopkins University Functional assay for identification of loss-of-function mutations in genes
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US9797010B2 (en) 2007-12-21 2017-10-24 President And Fellows Of Harvard College Systems and methods for nucleic acid sequencing
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
WO2009124255A2 (en) 2008-04-04 2009-10-08 Helicos Biosciences Corporation Methods for transcript analysis
US8093064B2 (en) 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
CN102124259B (zh) 2008-05-16 2015-12-16 哈佛大学 在包含微流体系统的流体系统中的阀和其它流动控制
CA2734521A1 (en) 2008-08-28 2010-03-04 Dermtech International Determining age ranges of skin samples
EP2210935A1 (en) 2009-01-19 2010-07-28 Deinove Methods for isolating bacteria
US9056299B2 (en) 2009-03-13 2015-06-16 President And Fellows Of Harvard College Scale-up of flow-focusing microfluidic devices
US20110319298A1 (en) * 2009-04-21 2011-12-29 Benner Steven A Differential detection of single nucleotide polymorphisms
EP4019977B1 (en) 2009-06-26 2024-11-20 President and Fellows of Harvard College Fluid injection
US20100330556A1 (en) * 2009-06-30 2010-12-30 Brian Jon Peter Genome analysis using a nicking endonuclease
WO2011038241A1 (en) * 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
EP2491123B1 (en) * 2009-10-20 2018-04-18 The Regents of The University of California Single molecule nucleic acid nanoparticles
EP2493619B1 (en) 2009-10-27 2018-12-19 President and Fellows of Harvard College Droplet creation techniques
WO2011055232A2 (en) * 2009-11-04 2011-05-12 Population Genetics Technologies Ltd. Base-by-base mutation screening
WO2011068088A1 (ja) 2009-12-04 2011-06-09 株式会社日立製作所 ２次元cDNAライブラリを用いた遺伝子発現解析方法
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2011086118A1 (en) 2010-01-14 2011-07-21 Deutsches Krebsforschungszentrum In vivo determination of the lokalization of dna double-stand breaks and application thereof
EP2539449B9 (en) 2010-02-26 2018-09-05 Qiagen GmbH Process for parallel isolation and/or purification of rna and dna
WO2011106724A2 (en) 2010-02-26 2011-09-01 Life Technologies Corporation Fast pcr for str genotyping
US20130109024A1 (en) 2010-05-28 2013-05-02 Sridharan Rajagopalan Obtaining analytes from a tissue specimen
EP2608878A4 (en) 2010-08-23 2017-11-15 President and Fellows of Harvard College Acoustic waves in microfluidics
US9982304B2 (en) 2010-09-03 2018-05-29 The Johns Hopkins University ARID1A and PPP2R1A mutations in cancer
WO2012048341A1 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
WO2012097353A1 (en) 2011-01-14 2012-07-19 Life Technologies Corporation Methods, compositions, and kits for detecting rare cells
CA2841430C (en) 2011-03-30 2018-12-04 Gnubio, Inc. Injection of multiple volumes into or out of droplets
EP2691540B1 (en) 2011-03-31 2016-01-20 GnuBIO, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
PL3246416T3 (pl) 2011-04-15 2024-09-30 The Johns Hopkins University Bezpieczny system sekwencjonowania
EP2707509A4 (en) 2011-05-12 2014-11-26 Netbio Inc METHODS AND COMPOSITIONS FOR RAPID MULTIPLEX AMPLIFICATION OF LOCI OF REPEATED SHORT SEQUENCES IN TANDEM
EP2714254B1 (en) 2011-05-23 2017-09-06 President and Fellows of Harvard College Control of emulsions, including multiple emulsions
US9617598B2 (en) 2011-05-27 2017-04-11 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
WO2013006793A2 (en) 2011-07-06 2013-01-10 Quest Diagnostics Investments Incorporated Direct amplification and detection of viral and bacterial pathogens
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
WO2013095737A2 (en) 2011-09-28 2013-06-27 President And Fellows Of Harvard College Systems and methods for droplet production and/or fluidic manipulation
SG11201401628WA (en) * 2011-10-19 2014-05-29 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
ES2730711T3 (es) 2011-11-03 2019-11-12 Qiagen Gaithersburg Inc Materiales y métodos para inmovilizar, aislar y concentrar células usando superficies carboxiladas
EP2776165A2 (en) 2011-11-07 2014-09-17 Illumina, Inc. Integrated sequencing apparatuses and methods of use
WO2013081864A1 (en) 2011-11-29 2013-06-06 Life Technologies Corporation Methods and compositions for multiplex pcr
KR20140122751A (ko) 2012-02-08 2014-10-20 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 유체 파열을 사용한 액적 형성
US20130309676A1 (en) 2012-05-18 2013-11-21 Alfred E. Mann Foundation For Scientific Research Biased n-mers identification methods, probes and systems for target amplification and detection
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US10058306B2 (en) 2012-06-22 2018-08-28 Preprogen, LLC Method for obtaining fetal cells and fetal cellular components
WO2014028378A2 (en) 2012-08-13 2014-02-20 The Regents Of The University Of California Methods and systems for detecting biological components
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9422535B2 (en) 2013-04-25 2016-08-23 Thermo Fisher Scientific Baltics Uab phi29 DNA polymerase mutants having increased thermostability and processivity
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
ES2764096T3 (es) * 2013-08-19 2020-06-02 Abbott Molecular Inc Bibliotecas de secuenciación de próxima generación
EP3039119A4 (en) 2013-08-27 2017-04-05 GnuBIO, Inc. Microfluidic devices and methods of their use
US20150099947A1 (en) 2013-10-04 2015-04-09 Access Business Group International Llc Skin youthfulness index, methods and applications thereof
US11339435B2 (en) 2013-10-18 2022-05-24 Molecular Loop Biosciences, Inc. Methods for copy number determination
JP6996844B2 (ja) 2014-02-27 2022-02-03 ジャンプコード ゲノミクス，インク． 体細胞可動要素の分析のための方法、およびその使用
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
US10232373B2 (en) 2014-06-16 2019-03-19 Bio-Rad Laboratories, Inc. Size alternating injection into drops to facilitate sorting
US10480021B2 (en) 2014-06-23 2019-11-19 Yale University Methods for closed chromatin mapping and DNA methylation analysis for single cells
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
CN112941065A (zh) 2014-07-21 2021-06-11 亿明达股份有限公司 使用crispr-cas系统的多核苷酸富集
ES2880335T3 (es) 2014-09-09 2021-11-24 Igenomx Int Genomics Corporation Métodos y composiciones para la preparación rápida de bibliotecas de ácidos nucleicos
US10434507B2 (en) 2014-10-22 2019-10-08 The Regents Of The University Of California High definition microdroplet printer
WO2016090273A1 (en) 2014-12-05 2016-06-09 Foundation Medicine, Inc. Multigene analysis of tumor samples
CN107208154A (zh) 2015-01-21 2017-09-26 新加坡科技研究局 单细胞rna和突变分析pcr(scrm‑pcr)：用于在单细胞水平上同时分析dna和rna的方法
JP2018508198A (ja) 2015-02-04 2018-03-29 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 別個の実体におけるバーコード付加による核酸のシーケンシング
WO2016123692A1 (en) 2015-02-04 2016-08-11 The University Of British Columbia Methods and devices for analyzing particles
US20180080070A1 (en) * 2015-04-08 2018-03-22 Saint Louis University Method for template-dependent multiple displacement amplification
US9771575B2 (en) 2015-06-19 2017-09-26 Agilent Technologies, Inc. Methods for on-array fragmentation and barcoding of DNA samples
CA3242290A1 (en) 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
DE212016000165U1 (de) 2015-08-07 2018-04-16 Poc Medical Systems Inc. Mikrofluidikvorrichtungen
WO2017088169A1 (en) 2015-11-27 2017-06-01 Coyote Bioscience Co., Ltd. Methods and systems for nucleic acid amplification
WO2017136520A1 (en) 2016-02-04 2017-08-10 President And Fellows Of Harvard College Mitochondrial genome editing and regulation
WO2017176834A2 (en) 2016-04-06 2017-10-12 University Of Florida Research Foundation, Incorporated Measurement of genomic age for predicting the risk of cancer
CA3023621C (en) 2016-05-13 2021-07-27 F. Hoffmann-La Roche Ag Protein-based sample collection matrices and devices
CN109074430B (zh) 2016-05-26 2022-03-29 贝克顿迪金森公司 分子标记计数调整方法
WO2017218727A1 (en) 2016-06-15 2017-12-21 President And Fellows Of Harvard College Methods for rule-based genome design
NL2017177B1 (en) 2016-07-15 2018-01-18 Seatechenergy Ip B V Installation and process for anaerobic digestion of organic material
CN113151423B (zh) 2016-08-05 2024-09-10 生物辐射实验室股份有限公司 第二链引导
CN110088290A (zh) 2016-08-10 2019-08-02 加利福尼亚大学董事会 在乳液微滴中结合多重置换扩增和pcr
KR102363716B1 (ko) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
EP3526348A4 (en) 2016-10-17 2020-06-24 Lociomics Corporation HIGH-RESOLUTION SPATIAL GENOMIC ANALYSIS OF TISSUES AND CELL AGGREGATES
EP3535418B1 (en) 2016-11-07 2021-12-15 Battelle Memorial Institute Nanoscale biochemical sample preparation and analysis
KR20230052995A (ko) 2016-12-19 2023-04-20 퀀텀-에스아이 인코포레이티드 시퀀싱 반응을 위한 중합 효소
CA3047328A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
WO2018129368A2 (en) 2017-01-06 2018-07-12 Editas Medicine, Inc. Methods of assessing nuclease cleavage
US11130991B2 (en) 2017-03-08 2021-09-28 The University Of Chicago Method for highly sensitive DNA methylation analysis
WO2018175500A1 (en) 2017-03-21 2018-09-27 Hexanomics, Inc. Sealed microwell assay
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
JP6938685B2 (ja) 2017-06-02 2021-09-22 ノースウェスタン ユニヴァーシティＮｏｒｔｈｗｅｓｔｅｒｎ Ｕｎｉｖｅｒｓｉｔｙ 表皮試料採取および感知のためのマイクロ流体システム
DK3981884T3 (da) 2017-06-07 2023-09-04 Univ Oregon Health & Science Enkeltcelle-helgenombiblioteker til methyleringssekventering
EP4446437A3 (en) 2017-08-01 2025-01-01 Illumina, Inc. Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
WO2019023924A1 (en) 2017-08-01 2019-02-07 Helitec Limited METHODS FOR ENRICHMENT AND DETERMINATION OF TARGET NUCLEOTIDE SEQUENCES
WO2019046644A1 (en) 2017-08-31 2019-03-07 Coopergenomics, Inc. SYSTEMS AND METHODS FOR DETECTION OF NOVO MUTATIONS IN HUMAN EMBRYOS
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084055A1 (en) 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
JP2021513359A (ja) 2018-01-08 2021-05-27 クレイドル ジェノミクス インコーポレイテッド 核酸単離のための方法およびキット
ES2983417T3 (es) 2018-01-29 2024-10-23 St Jude Childrens Res Hospital Inc Método para la amplificación de ácidos nucleicos
CN112005115A (zh) 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
KR102773714B1 (ko) 2018-02-13 2025-02-27 일루미나, 인코포레이티드 하이드로겔 비드를 사용한 dna 서열분석
EP3765596A4 (en) 2018-03-12 2022-04-06 Silicon Valley Scientific, Inc. METHOD AND APPARATUS FOR PROCESSING TISSUE AND OTHER SAMPLES FOR ENCODING CELLULAR SPATIAL POSITION INFORMATION
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
WO2019236478A1 (en) 2018-06-04 2019-12-12 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free nucleic acids
EP4249651B1 (en) 2018-08-20 2025-01-29 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
CA3115273C (en) 2018-10-05 2023-08-08 Coopergenomics, Inc. Systems and methods for identifying chromosomal abnormalities in an embryo
SG11202102703VA (en) 2018-10-26 2021-04-29 Illumina Inc Modulating polymer beads for dna processing
US11365441B2 (en) 2019-05-22 2022-06-21 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
JP7733582B2 (ja) 2019-06-17 2025-09-03 ジョージア テック リサーチ コーポレイション クラスター化粒子を分離するためのろ過ベースのシステムおよび方法
GB201909325D0 (en) 2019-06-28 2019-08-14 Cs Genetics Ltd Reagents and methods for analysis for microparticles
CN114466935B (zh) 2019-07-31 2025-01-17 铂赛基因组学公司 基因突变分析
WO2021022085A2 (en) 2019-07-31 2021-02-04 Bioskryb, Inc. Single cell analysis
WO2021097250A2 (en) 2019-11-14 2021-05-20 The Trustees Of Columbia University In The City Of New York Systems, methods, and compositions for generating multi-omic information from single cells
AU2021219665A1 (en) 2020-02-10 2022-09-01 BioSkryb Genomics, Inc. Phi29 mutants and use thereof
JP7809644B2 (ja) 2020-02-18 2026-02-02 ライフ テクノロジーズ コーポレーション ウイルス配列を検出するための組成物、キット、及び方法
EP4118233A1 (en) 2020-03-12 2023-01-18 New England Biolabs, Inc. A rapid diagnostic test for lamp
IL296492A (en) 2020-03-20 2022-11-01 Genentech Inc Systems and methods to track the evolution of single cells
CA3178445A1 (en) 2020-03-31 2021-10-07 BioSkryb Genomics, Inc. Detection of low abundance viruses
EP4146815A1 (en) 2020-05-07 2023-03-15 Bioskryb Genomics, Inc. Detection of low abundance nucleic acids
EP4334706A4 (en) 2021-05-05 2025-08-06 Singular Genomics Systems Inc MULTIOMICS ANALYSIS DEVICE AND METHODS OF USE THEREOF
CN117651611A (zh) 2021-05-07 2024-03-05 铂赛基因组学公司 生物分子的高通量分析
EP4367235A1 (en) 2021-07-06 2024-05-15 Singular Genomics Systems, Inc. Compositions and methods for detecting genetic features
US20240271210A1 (en) 2021-07-23 2024-08-15 BioSkryb Genomics, Inc. Spatial nucleic acid analysis
EP4388128A4 (en) 2021-08-16 2025-07-09 Bioskryb Genomics Inc EMBRYONIC NUCLEIC ACID ANALYSIS
WO2023172923A2 (en) 2022-03-08 2023-09-14 BioSkryb Genomics, Inc. Systems and methods relating to bioinformatics
WO2023212223A1 (en) 2022-04-28 2023-11-02 BioSkryb Genomics, Inc. Single cell multiomics
WO2023212222A1 (en) 2022-04-29 2023-11-02 Qualcomm Incorporated Enhanced measurement object configurations and procedures
CN119585443A (zh) 2022-05-05 2025-03-07 铂赛基因组学公司 原代模板定向扩增及其方法
WO2024026376A2 (en) 2022-07-27 2024-02-01 BioSkryb Genomics, Inc. Methods and systems for multiomic analysis
EP4594728A2 (en) 2022-09-28 2025-08-06 Bioskryb Genomics, Inc. Methods and compositions for fixed sample analysis
WO2024158720A2 (en) 2023-01-23 2024-08-02 BioSkryb Genomics, Inc. Fine needle aspiration methods
WO2025072326A1 (en) 2023-09-26 2025-04-03 BioSkryb Genomics, Inc. Methods, systems, and compositions for cfdna analysis
WO2025085821A1 (en) 2023-10-19 2025-04-24 BioSkryb Genomics, Inc. Methods, systems, and compositions for cell storage and analysis

Also Published As

Publication number Publication date
ES2983417T3 (es) 2024-10-23
US20230022451A1 (en) 2023-01-26
AU2023202656A1 (en) 2023-05-18
SG11202007225TA (en) 2020-08-28
KR20200138183A (ko) 2020-12-09
JP7457434B2 (ja) 2024-03-28
CN112041459B (zh) 2024-09-10
US11643682B2 (en) 2023-05-09
US11905553B2 (en) 2024-02-20
EP3746564B1 (en) 2023-01-04
US20200362394A1 (en) 2020-11-19
EP4183886A1 (en) 2023-05-24
ES2937927T3 (es) 2023-04-03
DK3746564T3 (da) 2023-01-30
US20240035080A1 (en) 2024-02-01
AU2023202656B2 (en) 2025-07-24
EP3746564A4 (en) 2021-10-27
IL276343B1 (en) 2024-06-01
IL312691A (en) 2024-07-01
DK4183886T3 (da) 2024-06-03
AU2025252655A1 (en) 2025-11-06
US12559794B2 (en) 2026-02-24
CA3089756A1 (en) 2019-08-01
JP2021511794A (ja) 2021-05-13
EP3746564A1 (en) 2020-12-09
AU2019212953A1 (en) 2020-08-27
EA202091673A1 (ru) 2021-02-03
PL4183886T3 (pl) 2024-09-02
KR102653725B1 (ko) 2024-04-01
WO2019148119A1 (en) 2019-08-01
IL276343A (en) 2020-09-30
AU2019212953B2 (en) 2023-02-02
IL276343B2 (en) 2024-10-01
US20250002988A1 (en) 2025-01-02
CN112041459A (zh) 2020-12-04
EP4183886B1 (en) 2024-04-17

Similar Documents

Publication Publication Date Title
PL3746564T3 (pl) Sposób amplifikacji kwasów nukleinowych
IL262409A (en) Improved methods for modification of target nucleic acids
EP3789503C0 (en) METHOD FOR AMPLIFYING NUCLEIC ACIDS
DK3523430T3 (da) Manipulerede nukleinsyremålrettede nukleinsyrer
IL271342A (en) Nucleic acid-guided nucleases
IL287468A (en) Nucleic acid constructs and methods for their manufacture
PL3445850T3 (pl) Sposoby otrzymywania jednoniciowego rna
DK3334841T3 (da) Fremgangsmåde til undersøgelse af nukleinsyrer
EP3784799C0 (en) METHOD FOR DETECTING NUCLEIC ACIDS
DK3371310T3 (da) Nucleinsyrekonstruerede targetnucleinsyrer
EP3916090C0 (en) ISOTACHOPHORESIS FOR THE PURIFICATION OF NUCLEIC ACIDS
DK3234200T3 (da) Fremgangsmåde til målrettet depletering af nukleinsyrer ved anvendelse af CRISPR/CAS-systemproteiner
DK3882357T3 (da) Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
DK3294885T3 (da) Fremgangsmåde til at fremstille rna
EP3175023C0 (en) METHOD FOR DETERMINING NUCLEIC ACIDS
EP3352795A4 (en) COMPOSITIONS AND METHODS OF TARGET NUCLEIC ACID MODIFICATION
SG10201702663PA (en) Moving heat blocks for amplification of nucleic acids
IL284197A (en) Targeted integration of nucleic acids
IL280772A (en) Multiplex detection of nucleic acids
IL287706A (en) Methods for detecting nucleic acid variants
EP3436605C0 (en) AMPLIFICATION OF TARGET SEQUENCES
EP3719142A4 (en) TARGET NUCLEIC ACID AMPLIFICATION PROCESS AND ASSOCIATED COMPOSITION
LT3417072T (lt) Nukleorūgšties taikinių foninės amplifikacijos eliminavimo būdas
KR101955708B9 (ko) 핵산 추출튜브 및 이를 이용한 핵산 추출방법
KR102358418B9 (ko) 스몰 rna 검출 방법