SG11202007225TA - Method for nucleic acid amplification - Google Patents

Method for nucleic acid amplification

Info

Publication number
SG11202007225TA
SG11202007225TA SG11202007225TA SG11202007225TA SG11202007225TA SG 11202007225T A SG11202007225T A SG 11202007225TA SG 11202007225T A SG11202007225T A SG 11202007225TA SG 11202007225T A SG11202007225T A SG 11202007225TA SG 11202007225T A SG11202007225T A SG 11202007225TA
Authority
SG
Singapore
Prior art keywords
nucleic acid
acid amplification
amplification
nucleic
acid
Prior art date
Application number
SG11202007225TA
Inventor
Charles Gawad
John Easton
Veronica Gonzalez-Pena
Original Assignee
St Jude Childrens Res Hospital Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by St Jude Childrens Res Hospital Inc filed Critical St Jude Childrens Res Hospital Inc
Publication of SG11202007225TA publication Critical patent/SG11202007225TA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
SG11202007225TA 2018-01-29 2019-01-28 Method for nucleic acid amplification SG11202007225TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862623471P 2018-01-29 2018-01-29
PCT/US2019/015452 WO2019148119A1 (en) 2018-01-29 2019-01-28 Method for nucleic acid amplification

Publications (1)

Publication Number Publication Date
SG11202007225TA true SG11202007225TA (en) 2020-08-28

Family

ID=67396233

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202007225TA SG11202007225TA (en) 2018-01-29 2019-01-28 Method for nucleic acid amplification

Country Status (14)

Country Link
US (3) US11905553B2 (en)
EP (2) EP4183886B1 (en)
JP (1) JP7457434B2 (en)
KR (1) KR102653725B1 (en)
CN (1) CN112041459A (en)
AU (2) AU2019212953B2 (en)
CA (1) CA3089756A1 (en)
DK (1) DK3746564T3 (en)
EA (1) EA202091673A1 (en)
ES (1) ES2937927T3 (en)
IL (1) IL276343A (en)
PL (1) PL3746564T3 (en)
SG (1) SG11202007225TA (en)
WO (1) WO2019148119A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112041459A (en) 2018-01-29 2020-12-04 圣祖德儿童研究医院 Nucleic acid amplification method
CA3149201A1 (en) * 2019-07-31 2021-02-04 BioSkryb Genomics, Inc. Genetic mutational analysis
US11795450B2 (en) * 2019-09-06 2023-10-24 Microsoft Technology Licensing, Llc Array-based enzymatic oligonucleotide synthesis
CN112831543B (en) * 2019-11-25 2023-08-29 中国科学院青岛生物能源与过程研究所 Single-cell whole genome amplification kit and application thereof
CA3170318A1 (en) * 2020-02-10 2021-08-19 BioSkryb Genomics, Inc. Phi29 mutants and use thereof
WO2021226205A1 (en) * 2020-05-07 2021-11-11 Bioskryb, Inc. Detection of low abundance nucleic acids
US11702683B2 (en) 2020-05-28 2023-07-18 Microsoft Technology Licensing, Llc De novo polynucleotide synthesis with substrate-bound polymerase
GB202111194D0 (en) 2021-08-03 2021-09-15 Cergentis B V Method
WO2023107453A1 (en) * 2021-12-06 2023-06-15 Chan Zuckerberg Biohub, Inc. Method for combined genome methylation and variation analyses
WO2023215524A2 (en) * 2022-05-05 2023-11-09 BioSkryb Genomics, Inc. Primary template-directed amplification and methods thereof
KR20240031934A (en) * 2022-09-01 2024-03-08 주식회사 키오믹스 Composition for selective amplifying multiple target DNA and the method of thereof
CN117343999B (en) * 2023-12-05 2024-03-01 上海锐赛循益生物技术有限公司 Nucleic acid amplification method based on left-side probe annealing and right-side probe annealing extension

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
EP0852004B1 (en) 1995-10-11 2011-01-19 Luminex Corporation Multiplexed analysis of clinical specimens
JP2001519538A (en) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
CA2260991C (en) 1997-11-18 2009-03-24 Bio-Rad Laboratories, Inc. Multiplex flow immunoassays with magnetic particles as solid phase
WO1999058724A1 (en) * 1998-05-08 1999-11-18 Life Technologies, Inc. A method for synthesizing a nucleic acid molecule using a ribonuclease
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6649414B1 (en) 1999-08-17 2003-11-18 Luminex Corporation Microparticles with multiple fluorescent signals and methods of using same
WO2001013119A1 (en) 1999-08-17 2001-02-22 Luminex Corporation Encapsulation of fluorescent particles
DK1218542T3 (en) * 1999-09-13 2004-08-02 Nugen Technologies Inc Methods and compositions for linear isothermal amplification of polynucleotide sequences
US8529743B2 (en) 2000-07-25 2013-09-10 The Regents Of The University Of California Electrowetting-driven micropumping
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
GB0102568D0 (en) 2001-02-01 2001-03-21 Magnetic Biosolutions Sweden A Method
EP1404825A2 (en) * 2001-06-27 2004-04-07 Roche Diagnostics GmbH A WALK-THROUGH TECHNIQUE FOR i IN VITRO /i RECOMBINATION OF POLYNUCLEOTIDE SEQUENCES
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US7163612B2 (en) 2001-11-26 2007-01-16 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
WO2004034013A2 (en) 2002-05-06 2004-04-22 Colorado State University Research Foundation Genotoxicity analysis
FR2841063B1 (en) 2002-06-18 2004-09-17 Commissariat Energie Atomique DEVICE FOR DISPLACING SMALL VOLUMES OF LIQUID ALONG A MICRO-CATENARY BY ELECTROSTATIC FORCES
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7547380B2 (en) 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
CN100478075C (en) 2003-11-17 2009-04-15 皇家飞利浦电子股份有限公司 System for manipulation of a body of fluid
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
CA2553025A1 (en) 2004-01-14 2005-07-28 Luminex Corporation Methods and systems for dynamic range expansion
WO2005073409A2 (en) 2004-01-26 2005-08-11 Applera Corporation Methods, compositions, and kits for amplifying and sequencing polynucleotides
FR2866493B1 (en) 2004-02-16 2010-08-20 Commissariat Energie Atomique DEVICE FOR CONTROLLING THE DISPLACEMENT OF A DROP BETWEEN TWO OR MORE SOLID SUBSTRATES
CN1950519A (en) 2004-02-27 2007-04-18 哈佛大学的校长及成员们 Polony fluorescent in situ sequencing beads
FR2872715B1 (en) 2004-07-08 2006-11-17 Commissariat Energie Atomique MICROREACTOR DROP
FR2872809B1 (en) 2004-07-09 2006-09-15 Commissariat Energie Atomique METHOD OF ADDRESSING ELECTRODES
WO2006073504A2 (en) 2004-08-04 2006-07-13 President And Fellows Of Harvard College Wobble sequencing
JP2006058031A (en) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp Chemical analyzer
EP1842211B1 (en) 2005-01-20 2010-08-04 Luminex Corporation Microspheres having fluorescent and magnetic properties
US7458661B2 (en) 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
PL1859330T3 (en) 2005-01-28 2013-01-31 Univ Duke Apparatuses and methods for manipulating droplets on a printed circuit board
US9040237B2 (en) * 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US20070023292A1 (en) 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
CN102621053B (en) 2005-09-21 2015-05-06 卢米尼克斯股份有限公司 Methods and systems for image data processing
CN103215248B (en) * 2005-10-03 2016-05-18 应用生物系统有限责任公司 For composition, method and the kit of amplification of nucleic acid
WO2007120208A2 (en) 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
WO2007103859A2 (en) 2006-03-03 2007-09-13 Luminex Corporation Methods, products, and kits for identifying an analyte in a sample
DE602006018794D1 (en) 2006-04-18 2011-01-20 Advanced Liquid Logic Inc BIOCHEMISTRY ON THE DREAM BASE
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
EP2040082A4 (en) 2006-07-10 2014-04-23 Hitachi High Tech Corp Liquid transfer device
US9266076B2 (en) 2006-11-02 2016-02-23 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US7893227B2 (en) * 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US7897737B2 (en) * 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
CN102851369B (en) 2006-12-13 2015-01-21 卢米耐克斯公司 Systems and methods for multiplex analysis of PCR in real time
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
CN101627308B (en) 2007-02-09 2013-08-14 先进流体逻辑公司 Droplet actuator devices and methods employing magnetic beads
WO2008116221A1 (en) 2007-03-22 2008-09-25 Advanced Liquid Logic, Inc. Bead sorting on a droplet actuator
WO2008134153A1 (en) 2007-04-23 2008-11-06 Advanced Liquid Logic, Inc. Bead-based multiplexed analytical methods and instrumentation
US8926811B2 (en) 2007-06-27 2015-01-06 Digital Biosystems Digital microfluidics based apparatus for heat-exchanging chemical processes
US8093064B2 (en) 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
US20110319298A1 (en) * 2009-04-21 2011-12-29 Benner Steven A Differential detection of single nucleotide polymorphisms
US20100330556A1 (en) * 2009-06-30 2010-12-30 Brian Jon Peter Genome analysis using a nicking endonuclease
WO2011038241A1 (en) * 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
WO2011050000A2 (en) 2009-10-20 2011-04-28 The Regents Of The University Of California Single molecule nucleic acid nanoparticles
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CA2814049C (en) 2010-10-08 2021-07-13 President And Fellows Of Harvard College High-throughput single cell barcoding
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
ES2625288T3 (en) 2011-04-15 2017-07-19 The Johns Hopkins University Secure Sequencing System
CA2835620C (en) 2011-05-12 2021-02-23 Netbio, Inc. Methods and compositions for rapid multiplex amplification of str loci
US20130309676A1 (en) 2012-05-18 2013-11-21 Alfred E. Mann Foundation For Scientific Research Biased n-mers identification methods, probes and systems for target amplification and detection
EP3514243B1 (en) 2012-05-21 2022-08-17 The Scripps Research Institute Methods of sample preparation
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
RU2698125C2 (en) * 2013-08-19 2019-08-22 Эбботт Молекьюлар Инк. Libraries for next generation sequencing
WO2015131110A1 (en) 2014-02-27 2015-09-03 Igenomx International Genomics Corporation Methods for analysis of somatic mobile elements, and uses thereof
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
EP3913066A1 (en) 2014-09-09 2021-11-24 Igenomx International Genomics Corporation Compositions for rapid nucleic acid library preparation
EP4112738A1 (en) * 2014-12-05 2023-01-04 Foundation Medicine, Inc. Multigene analysis of tumor samples
CN109074430B (en) 2016-05-26 2022-03-29 贝克顿迪金森公司 Molecular marker counting adjustment method
CN112041459A (en) 2018-01-29 2020-12-04 圣祖德儿童研究医院 Nucleic acid amplification method

Also Published As

Publication number Publication date
WO2019148119A1 (en) 2019-08-01
CA3089756A1 (en) 2019-08-01
US20240035080A1 (en) 2024-02-01
US11643682B2 (en) 2023-05-09
JP7457434B2 (en) 2024-03-28
AU2019212953A1 (en) 2020-08-27
EP3746564A4 (en) 2021-10-27
CN112041459A (en) 2020-12-04
DK3746564T3 (en) 2023-01-30
KR20200138183A (en) 2020-12-09
AU2023202656A1 (en) 2023-05-18
EA202091673A1 (en) 2021-02-03
US11905553B2 (en) 2024-02-20
KR102653725B1 (en) 2024-04-01
AU2019212953B2 (en) 2023-02-02
EP4183886A1 (en) 2023-05-24
EP3746564A1 (en) 2020-12-09
JP2021511794A (en) 2021-05-13
ES2937927T3 (en) 2023-04-03
IL276343A (en) 2020-09-30
EP4183886B1 (en) 2024-04-17
US20230022451A1 (en) 2023-01-26
US20200362394A1 (en) 2020-11-19
EP3746564B1 (en) 2023-01-04
PL3746564T3 (en) 2023-05-22

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