NZ545385A - Prion-specific peptide reagents - Google Patents

Info

Publication number

NZ545385A

NZ545385A NZ545385A NZ54538504A NZ545385A NZ 545385 A NZ545385 A NZ 545385A NZ 545385 A NZ545385 A NZ 545385A NZ 54538504 A NZ54538504 A NZ 54538504A NZ 545385 A NZ545385 A NZ 545385A

Authority

NZ

New Zealand

Prior art keywords

prion

pathogenic

peptide reagent

conformational

peptide

Prior art date

2003-08-13

Links

• Espacenet
• Global Dossier
• Discuss

• 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 663
• 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 560
• 102000029797 Prion Human genes 0.000 title claims abstract description 462
• 108091000054 Prion Proteins 0.000 title claims abstract description 462
• 230000001717 pathogenic effect Effects 0.000 claims abstract description 387
• 238000000034 method Methods 0.000 claims abstract description 205
• 108090000623 proteins and genes Proteins 0.000 claims abstract description 176
• 230000027455 binding Effects 0.000 claims abstract description 170
• 102000004169 proteins and genes Human genes 0.000 claims abstract description 161
• 201000010099 disease Diseases 0.000 claims abstract description 126
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 126
• 208000024777 Prion disease Diseases 0.000 claims abstract description 54
• 125000000539 amino acid group Chemical group 0.000 claims abstract description 19
• 238000000338 in vitro Methods 0.000 claims abstract description 16
• 239000000523 sample Substances 0.000 claims description 166
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 121
• 239000007787 solid Substances 0.000 claims description 108
• 239000000203 mixture Substances 0.000 claims description 77
• 150000001413 amino acids Chemical class 0.000 claims description 59
• 229920001184 polypeptide Polymers 0.000 claims description 51
• 239000011324 bead Substances 0.000 claims description 34
• 210000004369 blood Anatomy 0.000 claims description 34
• 239000008280 blood Substances 0.000 claims description 34
• 235000013305 food Nutrition 0.000 claims description 31
• 241000282414 Homo sapiens Species 0.000 claims description 29
• 239000012472 biological sample Substances 0.000 claims description 28
• 239000000463 material Substances 0.000 claims description 23
• 210000001519 tissue Anatomy 0.000 claims description 23
• 125000003275 alpha amino acid group Chemical group 0.000 claims description 20
• 239000003446 ligand Substances 0.000 claims description 20
• 229960005486 vaccine Drugs 0.000 claims description 19
• 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 claims description 17
• 108010049175 N-substituted Glycines Proteins 0.000 claims description 15
• 210000002966 serum Anatomy 0.000 claims description 15
• ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 14
• 210000002381 plasma Anatomy 0.000 claims description 14
• 230000036770 blood supply Effects 0.000 claims description 12
• 239000012528 membrane Substances 0.000 claims description 12
• 210000000056 organ Anatomy 0.000 claims description 12
• 210000001175 cerebrospinal fluid Anatomy 0.000 claims description 11
• 210000001772 blood platelet Anatomy 0.000 claims description 10
• 229920000037 Polyproline Polymers 0.000 claims description 9
• 210000004899 c-terminal region Anatomy 0.000 claims description 9
• 230000028993 immune response Effects 0.000 claims description 9
• 238000004519 manufacturing process Methods 0.000 claims description 9
• 210000000653 nervous system Anatomy 0.000 claims description 9
• 238000011282 treatment Methods 0.000 claims description 9
• 238000001574 biopsy Methods 0.000 claims description 8
• 239000003814 drug Substances 0.000 claims description 8
• 108010026466 polyproline Proteins 0.000 claims description 8
• 102000013566 Plasminogen Human genes 0.000 claims description 7
• 108010051456 Plasminogen Proteins 0.000 claims description 7
• 239000004793 Polystyrene Substances 0.000 claims description 7
• 230000000389 anti-prion effect Effects 0.000 claims description 7
• 229920002223 polystyrene Polymers 0.000 claims description 7
• 239000004816 latex Substances 0.000 claims description 6
• 229920000126 latex Polymers 0.000 claims description 6
• 239000012503 blood component Substances 0.000 claims description 5
• 239000000020 Nitrocellulose Substances 0.000 claims description 4
• 239000004677 Nylon Substances 0.000 claims description 4
• 229920006318 anionic polymer Polymers 0.000 claims description 4
• 210000005013 brain tissue Anatomy 0.000 claims description 4
• 239000003054 catalyst Substances 0.000 claims description 4
• 229920006317 cationic polymer Polymers 0.000 claims description 4
• 229920001220 nitrocellulos Polymers 0.000 claims description 4
• 229920001778 nylon Polymers 0.000 claims description 4
• 230000002265 prevention Effects 0.000 claims description 4
• 230000037452 priming Effects 0.000 claims description 4
• 210000002700 urine Anatomy 0.000 claims description 4
• 241000124008 Mammalia Species 0.000 claims description 3
• 238000011887 Necropsy Methods 0.000 claims description 3
• 210000000988 bone and bone Anatomy 0.000 claims description 3
• 210000003205 muscle Anatomy 0.000 claims description 3
• 229920002620 polyvinyl fluoride Polymers 0.000 claims description 3
• 230000002194 synthesizing effect Effects 0.000 claims description 3
• 241000371652 Curvularia clavata Species 0.000 claims 1
• 210000005260 human cell Anatomy 0.000 claims 1
• 235000018102 proteins Nutrition 0.000 description 85
• 235000001014 amino acid Nutrition 0.000 description 59
• 229940024606 amino acid Drugs 0.000 description 58
• 238000001514 detection method Methods 0.000 description 55
• 210000004027 cell Anatomy 0.000 description 50
• 102000040430 polynucleotide Human genes 0.000 description 43
• 108091033319 polynucleotide Proteins 0.000 description 43
• 239000002157 polynucleotide Substances 0.000 description 43
• 241001465754 Metazoa Species 0.000 description 38
• 239000000427 antigen Substances 0.000 description 38
• 102000036639 antigens Human genes 0.000 description 38
• 108091007433 antigens Proteins 0.000 description 38
• 238000003556 assay Methods 0.000 description 36
• -1 for example Proteins 0.000 description 33
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 32
• 108010043958 Peptoids Proteins 0.000 description 32
• 102000001708 Protein Isoforms Human genes 0.000 description 26
• 108010029485 Protein Isoforms Proteins 0.000 description 26
• 239000012634 fragment Substances 0.000 description 26
• 230000035772 mutation Effects 0.000 description 23
• 239000000306 component Substances 0.000 description 22
• 230000003993 interaction Effects 0.000 description 21
• 241000894007 species Species 0.000 description 21
• 239000002671 adjuvant Substances 0.000 description 20
• 210000004556 brain Anatomy 0.000 description 20
• 108020004414 DNA Proteins 0.000 description 19
• 230000014509 gene expression Effects 0.000 description 18
• 241000699666 Mus <mouse, genus> Species 0.000 description 17
• VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 16
• 229960002685 biotin Drugs 0.000 description 16
• 235000020958 biotin Nutrition 0.000 description 16
• 239000011616 biotin Substances 0.000 description 16
• 125000005647 linker group Chemical group 0.000 description 16
• 239000013598 vector Substances 0.000 description 16
• 239000004365 Protease Substances 0.000 description 15
• 239000000126 substance Substances 0.000 description 15
• 238000001262 western blot Methods 0.000 description 15
• 108091026890 Coding region Proteins 0.000 description 14
• 108091005804 Peptidases Proteins 0.000 description 14
• 102000035195 Peptidases Human genes 0.000 description 14
• 230000015572 biosynthetic process Effects 0.000 description 14
• ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 12
• 238000012217 deletion Methods 0.000 description 12
• 230000037430 deletion Effects 0.000 description 12
• 238000013461 design Methods 0.000 description 12
• 208000015181 infectious disease Diseases 0.000 description 12
• 230000004048 modification Effects 0.000 description 12
• 238000012986 modification Methods 0.000 description 12
• 150000007523 nucleic acids Chemical class 0.000 description 12
• 102000039446 nucleic acids Human genes 0.000 description 12
• 108020004707 nucleic acids Proteins 0.000 description 12
• 238000006467 substitution reaction Methods 0.000 description 12
• 230000001225 therapeutic effect Effects 0.000 description 12
• JOWMUWBEMDOCKB-UHFFFAOYSA-N 2-(cyclopropylmethylazaniumyl)acetate Chemical compound OC(=O)CNCC1CC1 JOWMUWBEMDOCKB-UHFFFAOYSA-N 0.000 description 11
• 238000002965 ELISA Methods 0.000 description 11
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 11
• 102100025818 Major prion protein Human genes 0.000 description 11
• 108091034117 Oligonucleotide Proteins 0.000 description 11
• 238000007792 addition Methods 0.000 description 11
• 239000011859 microparticle Substances 0.000 description 11
• 239000002773 nucleotide Substances 0.000 description 11
• 125000003729 nucleotide group Chemical group 0.000 description 11
• 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 11
• 239000000243 solution Substances 0.000 description 11
• 208000003407 Creutzfeldt-Jakob Syndrome Diseases 0.000 description 10
• 108010067770 Endopeptidase K Proteins 0.000 description 10
• 239000003795 chemical substances by application Substances 0.000 description 10
• 150000001875 compounds Chemical class 0.000 description 10
• 230000000694 effects Effects 0.000 description 10
• 239000000499 gel Substances 0.000 description 10
• 239000002245 particle Substances 0.000 description 10
• 241000283690 Bos taurus Species 0.000 description 9
• 102000004190 Enzymes Human genes 0.000 description 9
• 108090000790 Enzymes Proteins 0.000 description 9
• 241001494479 Pecora Species 0.000 description 9
• 108010090804 Streptavidin Proteins 0.000 description 9
• 208000018756 Variant Creutzfeldt-Jakob disease Diseases 0.000 description 9
• 241000251539 Vertebrata <Metazoa> Species 0.000 description 9
• 208000005881 bovine spongiform encephalopathy Diseases 0.000 description 9
• 229940088598 enzyme Drugs 0.000 description 9
• 210000004408 hybridoma Anatomy 0.000 description 9
• 239000000178 monomer Substances 0.000 description 9
• 238000010561 standard procedure Methods 0.000 description 9
• 208000010859 Creutzfeldt-Jakob disease Diseases 0.000 description 8
• 239000002202 Polyethylene glycol Substances 0.000 description 8
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
• 230000000890 antigenic effect Effects 0.000 description 8
• 229920001577 copolymer Polymers 0.000 description 8
• 238000010494 dissociation reaction Methods 0.000 description 8
• 230000005593 dissociations Effects 0.000 description 8
• 230000002163 immunogen Effects 0.000 description 8
• 229920001223 polyethylene glycol Polymers 0.000 description 8
• 229920000642 polymer Polymers 0.000 description 8
• 238000001556 precipitation Methods 0.000 description 8
• 235000019419 proteases Nutrition 0.000 description 8
• 238000000746 purification Methods 0.000 description 8
• 230000003612 virological effect Effects 0.000 description 8
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
• RRWZZMHRVSMLCT-UHFFFAOYSA-N 2-(butylazaniumyl)acetate Chemical compound CCCCNCC(O)=O RRWZZMHRVSMLCT-UHFFFAOYSA-N 0.000 description 7
• HEPOIJKOXBKKNJ-UHFFFAOYSA-N 2-(propan-2-ylazaniumyl)acetate Chemical compound CC(C)NCC(O)=O HEPOIJKOXBKKNJ-UHFFFAOYSA-N 0.000 description 7
• UOMFNVIFBSEWJZ-UHFFFAOYSA-N 2-[(3,5-dimethoxyphenyl)methylamino]acetic acid Chemical compound COC1=CC(CNCC(O)=O)=CC(OC)=C1 UOMFNVIFBSEWJZ-UHFFFAOYSA-N 0.000 description 7
• 102000002260 Alkaline Phosphatase Human genes 0.000 description 7
• 108020004774 Alkaline Phosphatase Proteins 0.000 description 7
• 208000020406 Creutzfeldt Jacob disease Diseases 0.000 description 7
• 208000003736 Gerstmann-Straussler-Scheinker Disease Diseases 0.000 description 7
• 206010072075 Gerstmann-Straussler-Scheinker syndrome Diseases 0.000 description 7
• 108060003951 Immunoglobulin Proteins 0.000 description 7
• 108091028043 Nucleic acid sequence Proteins 0.000 description 7
• 238000004458 analytical method Methods 0.000 description 7
• 239000003599 detergent Substances 0.000 description 7
• 230000006870 function Effects 0.000 description 7
• 230000003053 immunization Effects 0.000 description 7
• 102000018358 immunoglobulin Human genes 0.000 description 7
• 238000002347 injection Methods 0.000 description 7
• 239000007924 injection Substances 0.000 description 7
• 230000001105 regulatory effect Effects 0.000 description 7
• 208000008864 scrapie Diseases 0.000 description 7
• 238000012216 screening Methods 0.000 description 7
• 125000006850 spacer group Chemical group 0.000 description 7
• 238000003786 synthesis reaction Methods 0.000 description 7
• 238000013519 translation Methods 0.000 description 7
• 230000014616 translation Effects 0.000 description 7
• MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 6
• 241000588724 Escherichia coli Species 0.000 description 6
• 239000004471 Glycine Substances 0.000 description 6
• 108010001336 Horseradish Peroxidase Proteins 0.000 description 6
• CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 6
• 101710173438 Late L2 mu core protein Proteins 0.000 description 6
• 101100298534 Mus musculus Prnp gene Proteins 0.000 description 6
• 241000699670 Mus sp. Species 0.000 description 6
• 101710188315 Protein X Proteins 0.000 description 6
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 6
• 210000003719 b-lymphocyte Anatomy 0.000 description 6
• 239000002585 base Substances 0.000 description 6
• 239000000872 buffer Substances 0.000 description 6
• 239000000969 carrier Substances 0.000 description 6
• 230000001413 cellular effect Effects 0.000 description 6
• 238000006243 chemical reaction Methods 0.000 description 6
• 238000003745 diagnosis Methods 0.000 description 6
• 150000002148 esters Chemical class 0.000 description 6
• 239000013604 expression vector Substances 0.000 description 6
• 239000011521 glass Substances 0.000 description 6
• 230000001900 immune effect Effects 0.000 description 6
• 238000003018 immunoassay Methods 0.000 description 6
• 238000001727 in vivo Methods 0.000 description 6
• 230000002458 infectious effect Effects 0.000 description 6
• 238000010647 peptide synthesis reaction Methods 0.000 description 6
• 238000002360 preparation method Methods 0.000 description 6
• 239000000047 product Substances 0.000 description 6
• 230000000069 prophylactic effect Effects 0.000 description 6
• 238000012552 review Methods 0.000 description 6
• 239000000377 silicon dioxide Substances 0.000 description 6
• 239000011780 sodium chloride Substances 0.000 description 6
• 239000007790 solid phase Substances 0.000 description 6
• 210000000278 spinal cord Anatomy 0.000 description 6
• 239000000758 substrate Substances 0.000 description 6
• 238000012360 testing method Methods 0.000 description 6
• 108090001008 Avidin Proteins 0.000 description 5
• 241000894006 Bacteria Species 0.000 description 5
• 241000699800 Cricetinae Species 0.000 description 5
• 241000282412 Homo Species 0.000 description 5
• 108010021625 Immunoglobulin Fragments Proteins 0.000 description 5
• 102000008394 Immunoglobulin Fragments Human genes 0.000 description 5
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 5
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 5
• 102100038124 Plasminogen Human genes 0.000 description 5
• 108010076504 Protein Sorting Signals Proteins 0.000 description 5
• 241000700605 Viruses Species 0.000 description 5
• 238000013459 approach Methods 0.000 description 5
• 239000002299 complementary DNA Substances 0.000 description 5
• 239000000975 dye Substances 0.000 description 5
• 201000006061 fatal familial insomnia Diseases 0.000 description 5
• 238000001476 gene delivery Methods 0.000 description 5
• 150000004676 glycans Chemical class 0.000 description 5
• 238000002649 immunization Methods 0.000 description 5
• 238000011534 incubation Methods 0.000 description 5
• 230000000670 limiting effect Effects 0.000 description 5
• 239000002502 liposome Substances 0.000 description 5
• 239000007788 liquid Substances 0.000 description 5
• 229910052751 metal Inorganic materials 0.000 description 5
• 239000002184 metal Substances 0.000 description 5
• 229920002401 polyacrylamide Polymers 0.000 description 5
• 229920001282 polysaccharide Polymers 0.000 description 5
• 239000005017 polysaccharide Substances 0.000 description 5
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 5
• 239000001397 quillaja saponaria molina bark Substances 0.000 description 5
• 238000010188 recombinant method Methods 0.000 description 5
• 150000003839 salts Chemical class 0.000 description 5
• QAPSNMNOIOSXSQ-YNEHKIRRSA-N 1-[(2r,4s,5r)-4-[tert-butyl(dimethyl)silyl]oxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O[Si](C)(C)C(C)(C)C)C1 QAPSNMNOIOSXSQ-YNEHKIRRSA-N 0.000 description 4
• 241000271566 Aves Species 0.000 description 4
• 241000283707 Capra Species 0.000 description 4
• UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 4
• KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 4
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 4
• KSPIYJQBLVDRRI-UHFFFAOYSA-N N-methylisoleucine Chemical compound CCC(C)C(NC)C(O)=O KSPIYJQBLVDRRI-UHFFFAOYSA-N 0.000 description 4
• 241000282943 Odocoileus Species 0.000 description 4
• 102000007079 Peptide Fragments Human genes 0.000 description 4
• 108010033276 Peptide Fragments Proteins 0.000 description 4
• 229920003171 Poly (ethylene oxide) Polymers 0.000 description 4
• 210000001744 T-lymphocyte Anatomy 0.000 description 4
• 230000003321 amplification Effects 0.000 description 4
• 230000003302 anti-idiotype Effects 0.000 description 4
• 229940009098 aspartate Drugs 0.000 description 4
• 230000001580 bacterial effect Effects 0.000 description 4
• 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 4
• 125000002091 cationic group Chemical group 0.000 description 4
• 238000010367 cloning Methods 0.000 description 4
• 230000000295 complement effect Effects 0.000 description 4
• 230000008878 coupling Effects 0.000 description 4
• 238000010168 coupling process Methods 0.000 description 4
• 238000005859 coupling reaction Methods 0.000 description 4
• CTMZLDSMFCVUNX-VMIOUTBZSA-N cytidylyl-(3'->5')-guanosine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=C(C(N=C(N)N3)=O)N=C2)O)[C@@H](CO)O1 CTMZLDSMFCVUNX-VMIOUTBZSA-N 0.000 description 4
• 238000011161 development Methods 0.000 description 4
• 230000029087 digestion Effects 0.000 description 4
• 238000010790 dilution Methods 0.000 description 4
• 239000012895 dilution Substances 0.000 description 4
• 239000000539 dimer Substances 0.000 description 4
• PRAKJMSDJKAYCZ-UHFFFAOYSA-N dodecahydrosqualene Natural products CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 4
• 239000000839 emulsion Substances 0.000 description 4
• 230000002708 enhancing effect Effects 0.000 description 4
• 238000001114 immunoprecipitation Methods 0.000 description 4
• 238000007918 intramuscular administration Methods 0.000 description 4
• 229920002521 macromolecule Polymers 0.000 description 4
• 230000007246 mechanism Effects 0.000 description 4
• 108020004999 messenger RNA Proteins 0.000 description 4
• 229930182817 methionine Natural products 0.000 description 4
• 238000003199 nucleic acid amplification method Methods 0.000 description 4
• 229940066429 octoxynol Drugs 0.000 description 4
• 229920002113 octoxynol Polymers 0.000 description 4
• 238000002823 phage display Methods 0.000 description 4
• 229920000747 poly(lactic acid) Polymers 0.000 description 4
• 125000006239 protecting group Chemical group 0.000 description 4
• 239000011347 resin Substances 0.000 description 4
• 229920005989 resin Polymers 0.000 description 4
• 229930182490 saponin Natural products 0.000 description 4
• 150000007949 saponins Chemical class 0.000 description 4
• 238000002741 site-directed mutagenesis Methods 0.000 description 4
• 239000004094 surface-active agent Substances 0.000 description 4
• 230000009261 transgenic effect Effects 0.000 description 4
• FUOOLUPWFVMBKG-UHFFFAOYSA-N 2-Aminoisobutyric acid Chemical compound CC(C)(N)C(O)=O FUOOLUPWFVMBKG-UHFFFAOYSA-N 0.000 description 3
• 229920000936 Agarose Polymers 0.000 description 3
• 101710186708 Agglutinin Proteins 0.000 description 3
• DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 3
• 108010039939 Cell Wall Skeleton Proteins 0.000 description 3
• 108020004705 Codon Proteins 0.000 description 3
• 229920002307 Dextran Polymers 0.000 description 3
• 241000196324 Embryophyta Species 0.000 description 3
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
• 241000238631 Hexapoda Species 0.000 description 3
• 101710146024 Horcolin Proteins 0.000 description 3
• 108010065805 Interleukin-12 Proteins 0.000 description 3
• 108010002350 Interleukin-2 Proteins 0.000 description 3
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 3
• 101710189395 Lectin Proteins 0.000 description 3
• 239000004472 Lysine Substances 0.000 description 3
• 101710179758 Mannose-specific lectin Proteins 0.000 description 3
• 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 3
• 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 3
• 241000699660 Mus musculus Species 0.000 description 3
• 206010035226 Plasma cell myeloma Diseases 0.000 description 3
• 239000004743 Polypropylene Substances 0.000 description 3
• 241000700159 Rattus Species 0.000 description 3
• 108020004511 Recombinant DNA Proteins 0.000 description 3
• MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 3
• 230000024932 T cell mediated immunity Effects 0.000 description 3
• 102000003978 Tissue Plasminogen Activator Human genes 0.000 description 3
• 108090000373 Tissue Plasminogen Activator Proteins 0.000 description 3
• 241000700618 Vaccinia virus Species 0.000 description 3
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 3
• 230000002159 abnormal effect Effects 0.000 description 3
• 238000001042 affinity chromatography Methods 0.000 description 3
• 239000000910 agglutinin Substances 0.000 description 3
• 235000004279 alanine Nutrition 0.000 description 3
• 235000009582 asparagine Nutrition 0.000 description 3
• 229960001230 asparagine Drugs 0.000 description 3
• 239000010836 blood and blood product Substances 0.000 description 3
• 229940125691 blood product Drugs 0.000 description 3
• 210000004520 cell wall skeleton Anatomy 0.000 description 3
• 239000001913 cellulose Substances 0.000 description 3
• 229920002678 cellulose Polymers 0.000 description 3
• 230000008859 change Effects 0.000 description 3
• 208000017580 chronic wasting disease Diseases 0.000 description 3
• 238000003776 cleavage reaction Methods 0.000 description 3
• 230000001747 exhibiting effect Effects 0.000 description 3
• 229930195712 glutamate Natural products 0.000 description 3
• 230000013595 glycosylation Effects 0.000 description 3
• 238000006206 glycosylation reaction Methods 0.000 description 3
• 230000012010 growth Effects 0.000 description 3
• 238000004128 high performance liquid chromatography Methods 0.000 description 3
• 239000012510 hollow fiber Substances 0.000 description 3
• 238000009396 hybridization Methods 0.000 description 3
• 229940072221 immunoglobulins Drugs 0.000 description 3
• 230000003308 immunostimulating effect Effects 0.000 description 3
• 238000003780 insertion Methods 0.000 description 3
• 230000037431 insertion Effects 0.000 description 3
• 238000002955 isolation Methods 0.000 description 3
• 206010023497 kuru Diseases 0.000 description 3
• 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 3
• 239000013642 negative control Substances 0.000 description 3
• 238000006384 oligomerization reaction Methods 0.000 description 3
• 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 3
• 229920001155 polypropylene Polymers 0.000 description 3
• 229920000136 polysorbate Polymers 0.000 description 3
• 229920000053 polysorbate 80 Polymers 0.000 description 3
• 239000013641 positive control Substances 0.000 description 3
• 230000001376 precipitating effect Effects 0.000 description 3
• 230000008569 process Effects 0.000 description 3
• 238000012545 processing Methods 0.000 description 3
• 238000000159 protein binding assay Methods 0.000 description 3
• 230000007017 scission Effects 0.000 description 3
• 238000010532 solid phase synthesis reaction Methods 0.000 description 3
• 230000009870 specific binding Effects 0.000 description 3
• 229960000187 tissue plasminogen activator Drugs 0.000 description 3
• 238000001890 transfection Methods 0.000 description 3
• 238000012546 transfer Methods 0.000 description 3
• 238000011830 transgenic mouse model Methods 0.000 description 3
• 241000701447 unidentified baculovirus Species 0.000 description 3
• 239000004474 valine Substances 0.000 description 3
• 239000013603 viral vector Substances 0.000 description 3
• MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 2
• BVAUMRCGVHUWOZ-ZETCQYMHSA-N (2s)-2-(cyclohexylazaniumyl)propanoate Chemical compound OC(=O)[C@H](C)NC1CCCCC1 BVAUMRCGVHUWOZ-ZETCQYMHSA-N 0.000 description 2
• LJRDOKAZOAKLDU-UDXJMMFXSA-N (2s,3s,4r,5r,6r)-5-amino-2-(aminomethyl)-6-[(2r,3s,4r,5s)-5-[(1r,2r,3s,5r,6s)-3,5-diamino-2-[(2s,3r,4r,5s,6r)-3-amino-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-hydroxycyclohexyl]oxy-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]oxyoxane-3,4-diol;sulfuric ac Chemical compound OS(O)(=O)=O.N[C@@H]1[C@@H](O)[C@H](O)[C@H](CN)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](N)C[C@@H](N)[C@@H]2O)O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)O[C@@H]1CO LJRDOKAZOAKLDU-UDXJMMFXSA-N 0.000 description 2
• YYGNTYWPHWGJRM-UHFFFAOYSA-N (6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC=C(C)CCC=C(C)CCC=C(C)C YYGNTYWPHWGJRM-UHFFFAOYSA-N 0.000 description 2
• RKDVKSZUMVYZHH-UHFFFAOYSA-N 1,4-dioxane-2,5-dione Chemical compound O=C1COC(=O)CO1 RKDVKSZUMVYZHH-UHFFFAOYSA-N 0.000 description 2
• PAJPWUMXBYXFCZ-UHFFFAOYSA-N 1-aminocyclopropanecarboxylic acid Chemical compound OC(=O)C1(N)CC1 PAJPWUMXBYXFCZ-UHFFFAOYSA-N 0.000 description 2
• WHNPOQXWAMXPTA-UHFFFAOYSA-N 3-methylbut-2-enamide Chemical compound CC(C)=CC(N)=O WHNPOQXWAMXPTA-UHFFFAOYSA-N 0.000 description 2
• XWHHYOYVRVGJJY-QMMMGPOBSA-N 4-fluoro-L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(F)C=C1 XWHHYOYVRVGJJY-QMMMGPOBSA-N 0.000 description 2
• JJTUDXZGHPGLLC-IMJSIDKUSA-N 4511-42-6 Chemical compound C[C@@H]1OC(=O)[C@H](C)OC1=O JJTUDXZGHPGLLC-IMJSIDKUSA-N 0.000 description 2
• CJIJXIFQYOPWTF-UHFFFAOYSA-N 7-hydroxycoumarin Natural products O1C(=O)C=CC2=CC(O)=CC=C21 CJIJXIFQYOPWTF-UHFFFAOYSA-N 0.000 description 2
• 108010088751 Albumins Proteins 0.000 description 2
• 102000009027 Albumins Human genes 0.000 description 2
• 241000282979 Alces alces Species 0.000 description 2
• 241000272517 Anseriformes Species 0.000 description 2
• IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
• 244000063299 Bacillus subtilis Species 0.000 description 2
• 235000014469 Bacillus subtilis Nutrition 0.000 description 2
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
• 241000282693 Cercopithecidae Species 0.000 description 2
• LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
• 229920002101 Chitin Polymers 0.000 description 2
• 206010008631 Cholera Diseases 0.000 description 2
• RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
• 102000004127 Cytokines Human genes 0.000 description 2
• 108090000695 Cytokines Proteins 0.000 description 2
• 208000035240 Disease Resistance Diseases 0.000 description 2
• NIGWMJHCCYYCSF-UHFFFAOYSA-N Fenclonine Chemical compound OC(=O)C(N)CC1=CC=C(Cl)C=C1 NIGWMJHCCYYCSF-UHFFFAOYSA-N 0.000 description 2
• 108700028146 Genetic Enhancer Elements Proteins 0.000 description 2
• AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 2
• 229920002971 Heparan sulfate Polymers 0.000 description 2
• 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 2
• 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 2
• 102000014150 Interferons Human genes 0.000 description 2
• 108010050904 Interferons Proteins 0.000 description 2
• 102000004310 Ion Channels Human genes 0.000 description 2
• XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 2
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
• ZGUNAGUHMKGQNY-ZETCQYMHSA-N L-alpha-phenylglycine zwitterion Chemical compound OC(=O)[C@@H](N)C1=CC=CC=C1 ZGUNAGUHMKGQNY-ZETCQYMHSA-N 0.000 description 2
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 2
• HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
• AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
• LRQKBLKVPFOOQJ-YFKPBYRVSA-N L-norleucine Chemical compound CCCC[C@H]([NH3+])C([O-])=O LRQKBLKVPFOOQJ-YFKPBYRVSA-N 0.000 description 2
• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 2
• 102000002297 Laminin Receptors Human genes 0.000 description 2
• 108010000851 Laminin Receptors Proteins 0.000 description 2
• 108090001090 Lectins Proteins 0.000 description 2
• 102000004856 Lectins Human genes 0.000 description 2
• 241001529936 Murinae Species 0.000 description 2
• WRUZLCLJULHLEY-UHFFFAOYSA-N N-(p-hydroxyphenyl)glycine Chemical compound OC(=O)CNC1=CC=C(O)C=C1 WRUZLCLJULHLEY-UHFFFAOYSA-N 0.000 description 2
• 108700020354 N-acetylmuramyl-threonyl-isoglutamine Proteins 0.000 description 2
• 241000283973 Oryctolagus cuniculus Species 0.000 description 2
• 108010058846 Ovalbumin Proteins 0.000 description 2
• ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
• 229920000954 Polyglycolide Polymers 0.000 description 2
• 108010071690 Prealbumin Proteins 0.000 description 2
• 241000288906 Primates Species 0.000 description 2
• 241000283984 Rodentia Species 0.000 description 2
• 241000235070 Saccharomyces Species 0.000 description 2
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
• 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 2
• 238000012300 Sequence Analysis Methods 0.000 description 2
• 108010003723 Single-Domain Antibodies Proteins 0.000 description 2
• DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
• 108091081024 Start codon Proteins 0.000 description 2
• 241000187747 Streptomyces Species 0.000 description 2
• PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
• BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 description 2
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
• 239000004473 Threonine Substances 0.000 description 2
• IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
• 101710120037 Toxin CcdB Proteins 0.000 description 2
• 102000009190 Transthyretin Human genes 0.000 description 2
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
• 206010046865 Vaccinia virus infection Diseases 0.000 description 2
• XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 2
• 230000001594 aberrant effect Effects 0.000 description 2
• 230000002378 acidificating effect Effects 0.000 description 2
• 238000001261 affinity purification Methods 0.000 description 2
• 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 2
• 150000005215 alkyl ethers Chemical class 0.000 description 2
• 230000004075 alteration Effects 0.000 description 2
• AZDRQVAHHNSJOQ-UHFFFAOYSA-N alumane Chemical class [AlH3] AZDRQVAHHNSJOQ-UHFFFAOYSA-N 0.000 description 2
• 150000001408 amides Chemical class 0.000 description 2
• 150000001412 amines Chemical group 0.000 description 2
• 230000003942 amyloidogenic effect Effects 0.000 description 2
• 206010002022 amyloidosis Diseases 0.000 description 2
• 239000012491 analyte Substances 0.000 description 2
• 238000010171 animal model Methods 0.000 description 2
• 230000030741 antigen processing and presentation Effects 0.000 description 2
• 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 2
• 230000004888 barrier function Effects 0.000 description 2
• 230000004071 biological effect Effects 0.000 description 2
• 150000001642 boronic acid derivatives Chemical class 0.000 description 2
• 229940098773 bovine serum albumin Drugs 0.000 description 2
• 229910052799 carbon Inorganic materials 0.000 description 2
• 150000001732 carboxylic acid derivatives Chemical group 0.000 description 2
• 238000000423 cell based assay Methods 0.000 description 2
• 210000002421 cell wall Anatomy 0.000 description 2
• 238000005119 centrifugation Methods 0.000 description 2
• 238000004587 chromatography analysis Methods 0.000 description 2
• 238000004440 column chromatography Methods 0.000 description 2
• 238000011109 contamination Methods 0.000 description 2
• 238000007796 conventional method Methods 0.000 description 2
• 229910052802 copper Inorganic materials 0.000 description 2
• 239000010949 copper Substances 0.000 description 2
• 125000004122 cyclic group Chemical group 0.000 description 2
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
• 235000018417 cysteine Nutrition 0.000 description 2
• 125000001295 dansyl group Chemical group [H]C1=C([H])C(N(C([H])([H])[H])C([H])([H])[H])=C2C([H])=C([H])C([H])=C(C2=C1[H])S(*)(=O)=O 0.000 description 2
• 230000034994 death Effects 0.000 description 2
• 238000005202 decontamination Methods 0.000 description 2
• 230000003588 decontaminative effect Effects 0.000 description 2
• 239000012636 effector Substances 0.000 description 2
• 229920001971 elastomer Polymers 0.000 description 2
• 238000001962 electrophoresis Methods 0.000 description 2
• 238000005516 engineering process Methods 0.000 description 2
• 239000002532 enzyme inhibitor Substances 0.000 description 2
• 210000003743 erythrocyte Anatomy 0.000 description 2
• 238000002474 experimental method Methods 0.000 description 2
• 208000037957 feline spongiform encephalopathy Diseases 0.000 description 2
• 239000000835 fiber Substances 0.000 description 2
• GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
• 238000009472 formulation Methods 0.000 description 2
• 230000004927 fusion Effects 0.000 description 2
• 238000001415 gene therapy Methods 0.000 description 2
• 102000034356 gene-regulatory proteins Human genes 0.000 description 2
• 108091006104 gene-regulatory proteins Proteins 0.000 description 2
• 239000000833 heterodimer Substances 0.000 description 2
• 235000014304 histidine Nutrition 0.000 description 2
• 229920001600 hydrophobic polymer Polymers 0.000 description 2
• FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
• 210000000987 immune system Anatomy 0.000 description 2
• 230000001965 increasing effect Effects 0.000 description 2
• 230000001939 inductive effect Effects 0.000 description 2
• 230000003834 intracellular effect Effects 0.000 description 2
• 238000007912 intraperitoneal administration Methods 0.000 description 2
• 238000001990 intravenous administration Methods 0.000 description 2
• 238000004255 ion exchange chromatography Methods 0.000 description 2
• 229960000310 isoleucine Drugs 0.000 description 2
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 2
• 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 2
• 210000003734 kidney Anatomy 0.000 description 2
• 239000002523 lectin Substances 0.000 description 2
• 108010034897 lentil lectin Proteins 0.000 description 2
• 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 2
• 150000002632 lipids Chemical class 0.000 description 2
• HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 2
• 210000001165 lymph node Anatomy 0.000 description 2
• 210000004962 mammalian cell Anatomy 0.000 description 2
• 239000011159 matrix material Substances 0.000 description 2
• 230000001404 mediated effect Effects 0.000 description 2
• 239000002609 medium Substances 0.000 description 2
• 229910021645 metal ion Inorganic materials 0.000 description 2
• 235000013336 milk Nutrition 0.000 description 2
• 239000008267 milk Substances 0.000 description 2
• 210000004080 milk Anatomy 0.000 description 2
• 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 2
• 229910052757 nitrogen Inorganic materials 0.000 description 2
• 239000002736 nonionic surfactant Substances 0.000 description 2
• 238000005457 optimization Methods 0.000 description 2
• 229940092253 ovalbumin Drugs 0.000 description 2
• 238000007911 parenteral administration Methods 0.000 description 2
• 244000052769 pathogen Species 0.000 description 2
• 239000008188 pellet Substances 0.000 description 2
• 239000000816 peptidomimetic Substances 0.000 description 2
• 239000012071 phase Substances 0.000 description 2
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
• YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
• 229920000515 polycarbonate Polymers 0.000 description 2
• 239000004417 polycarbonate Substances 0.000 description 2
• 102000054765 polymorphisms of proteins Human genes 0.000 description 2
• 229920000056 polyoxyethylene ether Polymers 0.000 description 2
• 229940051841 polyoxyethylene ether Drugs 0.000 description 2
• 239000004800 polyvinyl chloride Substances 0.000 description 2
• 229920000915 polyvinyl chloride Polymers 0.000 description 2
• 238000001742 protein purification Methods 0.000 description 2
• 239000008262 pumice Substances 0.000 description 2
• 230000002285 radioactive effect Effects 0.000 description 2
• 230000004044 response Effects 0.000 description 2
• 230000001177 retroviral effect Effects 0.000 description 2
• PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
• 239000005060 rubber Substances 0.000 description 2
• 239000004576 sand Substances 0.000 description 2
• FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 2
• 230000003248 secreting effect Effects 0.000 description 2
• 230000028327 secretion Effects 0.000 description 2
• 238000000926 separation method Methods 0.000 description 2
• 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 2
• 229910052710 silicon Inorganic materials 0.000 description 2
• 239000010703 silicon Substances 0.000 description 2
• 238000001542 size-exclusion chromatography Methods 0.000 description 2
• 108010076805 snowdrop lectin Proteins 0.000 description 2
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
• 235000019333 sodium laurylsulphate Nutrition 0.000 description 2
• 238000001179 sorption measurement Methods 0.000 description 2
• 238000011895 specific detection Methods 0.000 description 2
• ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
• PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 description 2
• 210000000952 spleen Anatomy 0.000 description 2
• 210000004989 spleen cell Anatomy 0.000 description 2
• 230000002269 spontaneous effect Effects 0.000 description 2
• 229940031439 squalene Drugs 0.000 description 2
• TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 description 2
• 125000002653 sulfanylmethyl group Chemical group [H]SC([H])([H])[*] 0.000 description 2
• 239000006228 supernatant Substances 0.000 description 2
• 239000000725 suspension Substances 0.000 description 2
• 238000010998 test method Methods 0.000 description 2
• MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 2
• 238000013518 transcription Methods 0.000 description 2
• 230000035897 transcription Effects 0.000 description 2
• 230000005030 transcription termination Effects 0.000 description 2
• 230000009466 transformation Effects 0.000 description 2
• XETCRXVKJHBPMK-MJSODCSWSA-N trehalose 6,6'-dimycolate Chemical compound C([C@@H]1[C@H]([C@H](O)[C@@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](COC(=O)C(CCCCCCCCCCC3C(C3)CCCCCCCCCCCCCCCCCC)C(O)CCCCCCCCCCCCCCCCCCCCCCCCC)O2)O)O1)O)OC(=O)C(C(O)CCCCCCCCCCCCCCCCCCCCCCCCC)CCCCCCCCCCC1CC1CCCCCCCCCCCCCCCCCC XETCRXVKJHBPMK-MJSODCSWSA-N 0.000 description 2
• GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 2
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
• ORHBXUUXSCNDEV-UHFFFAOYSA-N umbelliferone Chemical compound C1=CC(=O)OC2=CC(O)=CC=C21 ORHBXUUXSCNDEV-UHFFFAOYSA-N 0.000 description 2
• HFTAFOQKODTIJY-UHFFFAOYSA-N umbelliferone Natural products Cc1cc2C=CC(=O)Oc2cc1OCC=CC(C)(C)O HFTAFOQKODTIJY-UHFFFAOYSA-N 0.000 description 2
• 208000007089 vaccinia Diseases 0.000 description 2
• 239000003981 vehicle Substances 0.000 description 2
• 238000003260 vortexing Methods 0.000 description 2
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
• PMJWDPGOWBRILU-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-[4-(2,5-dioxopyrrol-1-yl)phenyl]butanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCCC(C=C1)=CC=C1N1C(=O)C=CC1=O PMJWDPGOWBRILU-UHFFFAOYSA-N 0.000 description 1
• ZENNTZUZBRESKJ-ZETCQYMHSA-N (2s)-2-(1-benzothiophen-2-ylamino)propanoic acid Chemical compound C1=CC=C2SC(N[C@@H](C)C(O)=O)=CC2=C1 ZENNTZUZBRESKJ-ZETCQYMHSA-N 0.000 description 1
• IYKLZBIWFXPUCS-VIFPVBQESA-N (2s)-2-(naphthalen-1-ylamino)propanoic acid Chemical compound C1=CC=C2C(N[C@@H](C)C(O)=O)=CC=CC2=C1 IYKLZBIWFXPUCS-VIFPVBQESA-N 0.000 description 1
• RWLSBXBFZHDHHX-VIFPVBQESA-N (2s)-2-(naphthalen-2-ylamino)propanoic acid Chemical compound C1=CC=CC2=CC(N[C@@H](C)C(O)=O)=CC=C21 RWLSBXBFZHDHHX-VIFPVBQESA-N 0.000 description 1
• NPDBDJFLKKQMCM-SCSAIBSYSA-N (2s)-2-amino-3,3-dimethylbutanoic acid Chemical compound CC(C)(C)[C@H](N)C(O)=O NPDBDJFLKKQMCM-SCSAIBSYSA-N 0.000 description 1
• YHQZWWDVLJPRIF-JLHRHDQISA-N (4R)-4-[[(2S,3R)-2-[acetyl-[(3R,4R,5S,6R)-3-amino-4-[(1R)-1-carboxyethoxy]-5-hydroxy-6-(hydroxymethyl)oxan-2-yl]amino]-3-hydroxybutanoyl]amino]-5-amino-5-oxopentanoic acid Chemical compound C(C)(=O)N([C@@H]([C@H](O)C)C(=O)N[C@H](CCC(=O)O)C(N)=O)C1[C@H](N)[C@@H](O[C@@H](C(=O)O)C)[C@H](O)[C@H](O1)CO YHQZWWDVLJPRIF-JLHRHDQISA-N 0.000 description 1
• 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
• FQQREHKSHAYSMG-UHFFFAOYSA-N 1,2-dimethylacridine Chemical compound C1=CC=CC2=CC3=C(C)C(C)=CC=C3N=C21 FQQREHKSHAYSMG-UHFFFAOYSA-N 0.000 description 1
• 125000001917 2,4-dinitrophenyl group Chemical group [H]C1=C([H])C(=C([H])C(=C1*)[N+]([O-])=O)[N+]([O-])=O 0.000 description 1
• QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
• AXAVXPMQTGXXJZ-UHFFFAOYSA-N 2-aminoacetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol Chemical compound NCC(O)=O.OCC(N)(CO)CO AXAVXPMQTGXXJZ-UHFFFAOYSA-N 0.000 description 1
• WTOFYLAWDLQMBZ-UHFFFAOYSA-N 2-azaniumyl-3-thiophen-2-ylpropanoate Chemical compound OC(=O)C(N)CC1=CC=CS1 WTOFYLAWDLQMBZ-UHFFFAOYSA-N 0.000 description 1
• BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
• UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 1
• 125000004042 4-aminobutyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])N([H])[H] 0.000 description 1
• TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 1
• 241000256118 Aedes aegypti Species 0.000 description 1
• 244000003363 Allium ursinum Species 0.000 description 1
• 235000005336 Allium ursinum Nutrition 0.000 description 1
• 241000710929 Alphavirus Species 0.000 description 1
• 239000005995 Aluminium silicate Substances 0.000 description 1
• 208000024827 Alzheimer disease Diseases 0.000 description 1
• 208000037259 Amyloid Plaque Diseases 0.000 description 1
• 102000009091 Amyloidogenic Proteins Human genes 0.000 description 1
• 108010048112 Amyloidogenic Proteins Proteins 0.000 description 1
• 206010002023 Amyloidoses Diseases 0.000 description 1
• 241000256844 Apis mellifera Species 0.000 description 1
• 239000004475 Arginine Substances 0.000 description 1
• 206010003445 Ascites Diseases 0.000 description 1
• 102000007370 Ataxin2 Human genes 0.000 description 1
• 108010032951 Ataxin2 Proteins 0.000 description 1
• 201000001320 Atherosclerosis Diseases 0.000 description 1
• 101800001288 Atrial natriuretic factor Proteins 0.000 description 1
• 101800001890 Atrial natriuretic peptide Proteins 0.000 description 1
• 102400001282 Atrial natriuretic peptide Human genes 0.000 description 1
• 241001203868 Autographa californica Species 0.000 description 1
• 241000193830 Bacillus <bacterium> Species 0.000 description 1
• DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
• LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
• 241000255789 Bombyx mori Species 0.000 description 1
• 241000701822 Bovine papillomavirus Species 0.000 description 1
• 101710098275 C-X-C motif chemokine 10 Proteins 0.000 description 1
• XMWRBQBLMFGWIX-UHFFFAOYSA-N C60 fullerene Chemical class C12=C3C(C4=C56)=C7C8=C5C5=C9C%10=C6C6=C4C1=C1C4=C6C6=C%10C%10=C9C9=C%11C5=C8C5=C8C7=C3C3=C7C2=C1C1=C2C4=C6C4=C%10C6=C9C9=C%11C5=C5C8=C3C3=C7C1=C1C2=C4C6=C2C9=C5C3=C12 XMWRBQBLMFGWIX-UHFFFAOYSA-N 0.000 description 1
• 241000178270 Canarypox virus Species 0.000 description 1
• 241000222122 Candida albicans Species 0.000 description 1
• 241000222128 Candida maltosa Species 0.000 description 1
• 241000282472 Canis lupus familiaris Species 0.000 description 1
• 206010007509 Cardiac amyloidosis Diseases 0.000 description 1
• 206010007559 Cardiac failure congestive Diseases 0.000 description 1
• 102000014914 Carrier Proteins Human genes 0.000 description 1
• 241000700198 Cavia Species 0.000 description 1
• 241000282994 Cervidae Species 0.000 description 1
• 108010055166 Chemokine CCL5 Proteins 0.000 description 1
• 102000001327 Chemokine CCL5 Human genes 0.000 description 1
• 102000019034 Chemokines Human genes 0.000 description 1
• 108010012236 Chemokines Proteins 0.000 description 1
• 229920001661 Chitosan Polymers 0.000 description 1
• 241000557626 Corvus corax Species 0.000 description 1
• 241000699802 Cricetulus griseus Species 0.000 description 1
• 241000450599 DNA viruses Species 0.000 description 1
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
• 241000283014 Dama Species 0.000 description 1
• 241000702421 Dependoparvovirus Species 0.000 description 1
• BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
• 108010016626 Dipeptides Proteins 0.000 description 1
• 241000255601 Drosophila melanogaster Species 0.000 description 1
• 238000012286 ELISA Assay Methods 0.000 description 1
• 101100001670 Emericella variicolor andE gene Proteins 0.000 description 1
• 101710146739 Enterotoxin Proteins 0.000 description 1
• 239000004593 Epoxy Substances 0.000 description 1
• 241000283086 Equidae Species 0.000 description 1
• 241001331845 Equus asinus x caballus Species 0.000 description 1
• 241000283073 Equus caballus Species 0.000 description 1
• 101000686777 Escherichia phage T7 T7 RNA polymerase Proteins 0.000 description 1
• 108700024394 Exon Proteins 0.000 description 1
• 206010016202 Familial Amyloidosis Diseases 0.000 description 1
• 241000282326 Felis catus Species 0.000 description 1
• 208000000666 Fowlpox Diseases 0.000 description 1
• 241000700662 Fowlpox virus Species 0.000 description 1
• 201000011240 Frontotemporal dementia Diseases 0.000 description 1
• 241000272496 Galliformes Species 0.000 description 1
• 241000287828 Gallus gallus Species 0.000 description 1
• 102000004878 Gelsolin Human genes 0.000 description 1
• 108090001064 Gelsolin Proteins 0.000 description 1
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
• 239000004366 Glucose oxidase Substances 0.000 description 1
• 108010015776 Glucose oxidase Proteins 0.000 description 1
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
• 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
• 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 1
• 206010019280 Heart failures Diseases 0.000 description 1
• 208000032843 Hemorrhage Diseases 0.000 description 1
• SGCGMORCWLEJNZ-UWVGGRQHSA-N His-His Chemical compound C([C@H]([NH3+])C(=O)N[C@@H](CC=1NC=NC=1)C([O-])=O)C1=CN=CN1 SGCGMORCWLEJNZ-UWVGGRQHSA-N 0.000 description 1
• 241001272567 Hominoidea Species 0.000 description 1
• 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
• CPELXLSAUQHCOX-UHFFFAOYSA-N Hydrogen bromide Chemical class Br CPELXLSAUQHCOX-UHFFFAOYSA-N 0.000 description 1
• PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
• UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
• 235000003332 Ilex aquifolium Nutrition 0.000 description 1
• 241000209027 Ilex aquifolium Species 0.000 description 1
• 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
• 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 1
• 102000008070 Interferon-gamma Human genes 0.000 description 1
• 108010074328 Interferon-gamma Proteins 0.000 description 1
• 108090000978 Interleukin-4 Proteins 0.000 description 1
• 108010002616 Interleukin-5 Proteins 0.000 description 1
• 108090001005 Interleukin-6 Proteins 0.000 description 1
• 108010002586 Interleukin-7 Proteins 0.000 description 1
• 108010063738 Interleukins Proteins 0.000 description 1
• 102000015696 Interleukins Human genes 0.000 description 1
• 102000036770 Islet Amyloid Polypeptide Human genes 0.000 description 1
• 108010041872 Islet Amyloid Polypeptide Proteins 0.000 description 1
• 244000285963 Kluyveromyces fragilis Species 0.000 description 1
• 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 1
• 241001138401 Kluyveromyces lactis Species 0.000 description 1
• 241000235058 Komagataella pastoris Species 0.000 description 1
• GZYFIMLSHBLMKF-REOHCLBHSA-N L-Albizziine Chemical compound OC(=O)[C@@H](N)CNC(N)=O GZYFIMLSHBLMKF-REOHCLBHSA-N 0.000 description 1
• QUOGESRFPZDMMT-UHFFFAOYSA-N L-Homoarginine Natural products OC(=O)C(N)CCCCNC(N)=N QUOGESRFPZDMMT-UHFFFAOYSA-N 0.000 description 1
• AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
• GZYFIMLSHBLMKF-UHFFFAOYSA-N L-albizziine Natural products OC(=O)C(N)CNC(N)=O GZYFIMLSHBLMKF-UHFFFAOYSA-N 0.000 description 1
• DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
• ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
• QUOGESRFPZDMMT-YFKPBYRVSA-N L-homoarginine Chemical compound OC(=O)[C@@H](N)CCCCNC(N)=N QUOGESRFPZDMMT-YFKPBYRVSA-N 0.000 description 1
• FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
• 235000019687 Lamb Nutrition 0.000 description 1
• 108010074338 Lymphokines Proteins 0.000 description 1
• 102000008072 Lymphokines Human genes 0.000 description 1
• 101150111724 MEP1 gene Proteins 0.000 description 1
• 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 1
• 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
• 208000009018 Medullary thyroid cancer Diseases 0.000 description 1
• 108010036176 Melitten Proteins 0.000 description 1
• 241000699673 Mesocricetus auratus Species 0.000 description 1
• 208000034578 Multiple myelomas Diseases 0.000 description 1
• 102000016943 Muramidase Human genes 0.000 description 1
• 108010014251 Muramidase Proteins 0.000 description 1
• 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
• 238000005481 NMR spectroscopy Methods 0.000 description 1
• 241001532689 Narcissus pseudonarcissus Species 0.000 description 1
• 241001644525 Nastus productus Species 0.000 description 1
• 102000005348 Neuraminidase Human genes 0.000 description 1
• 108010006232 Neuraminidase Proteins 0.000 description 1
• 102000008763 Neurofilament Proteins Human genes 0.000 description 1
• 108010088373 Neurofilament Proteins Proteins 0.000 description 1
• 108091005461 Nucleic proteins Proteins 0.000 description 1
• 241000320412 Ogataea angusta Species 0.000 description 1
• 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
• 108010038807 Oligopeptides Proteins 0.000 description 1
• 102000015636 Oligopeptides Human genes 0.000 description 1
• AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
• UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
• 238000012408 PCR amplification Methods 0.000 description 1
• 229910019142 PO4 Inorganic materials 0.000 description 1
• 239000002033 PVDF binder Substances 0.000 description 1
• 108091081548 Palindromic sequence Proteins 0.000 description 1
• 241000282579 Pan Species 0.000 description 1
• 108090000526 Papain Proteins 0.000 description 1
• 208000002774 Paraproteinemias Diseases 0.000 description 1
• 208000018737 Parkinson disease Diseases 0.000 description 1
• 102000057297 Pepsin A Human genes 0.000 description 1
• 108090000284 Pepsin A Proteins 0.000 description 1
• 241000286209 Phasianidae Species 0.000 description 1
• 241000235648 Pichia Species 0.000 description 1
• 208000000609 Pick Disease of the Brain Diseases 0.000 description 1
• 240000004713 Pisum sativum Species 0.000 description 1
• 235000010582 Pisum sativum Nutrition 0.000 description 1
• 241000276498 Pollachius virens Species 0.000 description 1
• 229920002732 Polyanhydride Polymers 0.000 description 1
• 239000004698 Polyethylene Substances 0.000 description 1
• 239000004642 Polyimide Substances 0.000 description 1
• 108010039918 Polylysine Proteins 0.000 description 1
• 206010036105 Polyneuropathy Diseases 0.000 description 1
• 229920001710 Polyorthoester Polymers 0.000 description 1
• 239000004721 Polyphenylene oxide Substances 0.000 description 1
• 229920000388 Polyphosphate Polymers 0.000 description 1
• 108700021402 PrP 27-30 Proteins 0.000 description 1
• 108010007288 PrPSc Proteins Proteins 0.000 description 1
• 108010048233 Procalcitonin Proteins 0.000 description 1
• 239000012722 SDS sample buffer Substances 0.000 description 1
• 108010077895 Sarcosine Proteins 0.000 description 1
• 102000040739 Secretory proteins Human genes 0.000 description 1
• 108091058545 Secretory proteins Proteins 0.000 description 1
• 102000054727 Serum Amyloid A Human genes 0.000 description 1
• 108700028909 Serum Amyloid A Proteins 0.000 description 1
• 241000702208 Shigella phage SfX Species 0.000 description 1
• PRXRUNOAOLTIEF-ADSICKODSA-N Sorbitan trioleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](OC(=O)CCCCCCC\C=C/CCCCCCCC)[C@H]1OC[C@H](O)[C@H]1OC(=O)CCCCCCC\C=C/CCCCCCCC PRXRUNOAOLTIEF-ADSICKODSA-N 0.000 description 1
• 241000256251 Spodoptera frugiperda Species 0.000 description 1
• 229930182558 Sterol Natural products 0.000 description 1
• 241000194017 Streptococcus Species 0.000 description 1
• 241000282887 Suidae Species 0.000 description 1
• QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
• 206010043376 Tetanus Diseases 0.000 description 1
• 108010034949 Thyroglobulin Proteins 0.000 description 1
• 102000009843 Thyroglobulin Human genes 0.000 description 1
• 241000255993 Trichoplusia ni Species 0.000 description 1
• WGLPBDUCMAPZCE-UHFFFAOYSA-N Trioxochromium Chemical compound O=[Cr](=O)=O WGLPBDUCMAPZCE-UHFFFAOYSA-N 0.000 description 1
• 239000007983 Tris buffer Substances 0.000 description 1
• 108090000631 Trypsin Proteins 0.000 description 1
• 102000004142 Trypsin Human genes 0.000 description 1
• 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
• 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 1
• 108010046334 Urease Proteins 0.000 description 1
• 241000726445 Viroids Species 0.000 description 1
• 241000235015 Yarrowia lipolytica Species 0.000 description 1
• 150000001242 acetic acid derivatives Chemical class 0.000 description 1
• 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
• 230000021736 acetylation Effects 0.000 description 1
• 238000006640 acetylation reaction Methods 0.000 description 1
• 239000002253 acid Substances 0.000 description 1
• NIXOWILDQLNWCW-UHFFFAOYSA-M acrylate group Chemical group C(C=C)(=O)[O-] NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 description 1
• 230000004913 activation Effects 0.000 description 1
• 230000001464 adherent effect Effects 0.000 description 1
• 230000000240 adjuvant effect Effects 0.000 description 1
• 238000012382 advanced drug delivery Methods 0.000 description 1
• 230000004931 aggregating effect Effects 0.000 description 1
• 238000013019 agitation Methods 0.000 description 1
• 238000012867 alanine scanning Methods 0.000 description 1
• 239000003513 alkali Substances 0.000 description 1
• 125000004183 alkoxy alkyl group Chemical group 0.000 description 1
• 125000003545 alkoxy group Chemical group 0.000 description 1
• 125000002947 alkylene group Chemical group 0.000 description 1
• OJYGBLRPYBAHRT-UHFFFAOYSA-N alphachloralose Chemical compound O1C(C(Cl)(Cl)Cl)OC2C(O)C(C(O)CO)OC21 OJYGBLRPYBAHRT-UHFFFAOYSA-N 0.000 description 1
• 229910052782 aluminium Inorganic materials 0.000 description 1
• WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
• ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
• 235000012211 aluminium silicate Nutrition 0.000 description 1
• 229940047712 aluminum hydroxyphosphate Drugs 0.000 description 1
• 238000010976 amide bond formation reaction Methods 0.000 description 1
• 125000003368 amide group Chemical group 0.000 description 1
• 229960003896 aminopterin Drugs 0.000 description 1
• 210000004102 animal cell Anatomy 0.000 description 1
• 125000000129 anionic group Chemical group 0.000 description 1
• 239000005557 antagonist Substances 0.000 description 1
• 230000010056 antibody-dependent cellular cytotoxicity Effects 0.000 description 1
• 210000000628 antibody-producing cell Anatomy 0.000 description 1
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
• 235000003704 aspartic acid Nutrition 0.000 description 1
• 239000012131 assay buffer Substances 0.000 description 1
• 230000000712 assembly Effects 0.000 description 1
• 238000000429 assembly Methods 0.000 description 1
• 125000004429 atom Chemical group 0.000 description 1
• 235000015278 beef Nutrition 0.000 description 1
• 239000000440 bentonite Substances 0.000 description 1
• 229910000278 bentonite Inorganic materials 0.000 description 1
• SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
• 150000001558 benzoic acid derivatives Chemical class 0.000 description 1
• 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
• 102000015736 beta 2-Microglobulin Human genes 0.000 description 1
• 108010081355 beta 2-Microglobulin Proteins 0.000 description 1
• WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
• 102000005936 beta-Galactosidase Human genes 0.000 description 1
• 108010005774 beta-Galactosidase Proteins 0.000 description 1
• IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
• 230000001588 bifunctional effect Effects 0.000 description 1
• 239000003833 bile salt Substances 0.000 description 1
• 229940093761 bile salts Drugs 0.000 description 1
• 108091008324 binding proteins Proteins 0.000 description 1
• 230000008827 biological function Effects 0.000 description 1
• 230000033228 biological regulation Effects 0.000 description 1
• 230000005540 biological transmission Effects 0.000 description 1
• 230000000740 bleeding effect Effects 0.000 description 1
• 238000006664 bond formation reaction Methods 0.000 description 1
• ZADPBFCGQRWHPN-UHFFFAOYSA-N boronic acid Chemical compound OBO ZADPBFCGQRWHPN-UHFFFAOYSA-N 0.000 description 1
• 244000309464 bull Species 0.000 description 1
• 239000001506 calcium phosphate Substances 0.000 description 1
• 229910000389 calcium phosphate Inorganic materials 0.000 description 1
• 235000011010 calcium phosphates Nutrition 0.000 description 1
• 239000004295 calcium sulphite Substances 0.000 description 1
• 229940095731 candida albicans Drugs 0.000 description 1
• 239000002775 capsule Substances 0.000 description 1
• 125000004432 carbon atom Chemical group C* 0.000 description 1
• 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
• 125000004181 carboxyalkyl group Chemical group 0.000 description 1
• 230000000747 cardiac effect Effects 0.000 description 1
• NSQLIUXCMFBZME-MPVJKSABSA-N carperitide Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CSSC[C@@H](C(=O)N1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)=O)[C@@H](C)CC)C1=CC=CC=C1 NSQLIUXCMFBZME-MPVJKSABSA-N 0.000 description 1
• 238000004113 cell culture Methods 0.000 description 1
• 230000007910 cell fusion Effects 0.000 description 1
• 210000000170 cell membrane Anatomy 0.000 description 1
• 239000006285 cell suspension Substances 0.000 description 1
• 230000033077 cellular process Effects 0.000 description 1
• 230000002490 cerebral effect Effects 0.000 description 1
• 235000013330 chicken meat Nutrition 0.000 description 1
• 208000037976 chronic inflammation Diseases 0.000 description 1
• 230000006020 chronic inflammation Effects 0.000 description 1
• 208000020832 chronic kidney disease Diseases 0.000 description 1
• 208000022831 chronic renal failure syndrome Diseases 0.000 description 1
• 239000003541 chymotrypsin inhibitor Substances 0.000 description 1
• 239000013599 cloning vector Substances 0.000 description 1
• 230000002860 competitive effect Effects 0.000 description 1
• 238000004590 computer program Methods 0.000 description 1
• 238000009833 condensation Methods 0.000 description 1
• 230000005494 condensation Effects 0.000 description 1
• 230000008876 conformational transition Effects 0.000 description 1
• IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 1
• 238000010276 construction Methods 0.000 description 1
• 238000005100 correlation spectroscopy Methods 0.000 description 1
• 239000002537 cosmetic Substances 0.000 description 1
• 239000013078 crystal Substances 0.000 description 1
• 125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 description 1
• 210000000805 cytoplasm Anatomy 0.000 description 1
• 230000001086 cytosolic effect Effects 0.000 description 1
• 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 1
• 230000006378 damage Effects 0.000 description 1
• 230000006837 decompression Effects 0.000 description 1
• 230000002939 deleterious effect Effects 0.000 description 1
• 239000000412 dendrimer Substances 0.000 description 1
• 229920000736 dendritic polymer Polymers 0.000 description 1
• 230000008021 deposition Effects 0.000 description 1
• 238000010511 deprotection reaction Methods 0.000 description 1
• 238000001212 derivatisation Methods 0.000 description 1
• 239000008121 dextrose Substances 0.000 description 1
• 238000002405 diagnostic procedure Methods 0.000 description 1
• 230000004069 differentiation Effects 0.000 description 1
• 238000002050 diffraction method Methods 0.000 description 1
• 206010013023 diphtheria Diseases 0.000 description 1
• 231100000676 disease causative agent Toxicity 0.000 description 1
• KPUWHANPEXNPJT-UHFFFAOYSA-N disiloxane Chemical class [SiH3]O[SiH3] KPUWHANPEXNPJT-UHFFFAOYSA-N 0.000 description 1
• PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
• 229940079593 drug Drugs 0.000 description 1
• 241001493065 dsRNA viruses Species 0.000 description 1
• 238000002003 electron diffraction Methods 0.000 description 1
• 239000003995 emulsifying agent Substances 0.000 description 1
• 230000001804 emulsifying effect Effects 0.000 description 1
• 239000003623 enhancer Substances 0.000 description 1
• 239000000147 enterotoxin Substances 0.000 description 1
• 231100000655 enterotoxin Toxicity 0.000 description 1
• 125000003700 epoxy group Chemical group 0.000 description 1
• 125000000816 ethylene group Chemical group [H]C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
• 210000003527 eukaryotic cell Anatomy 0.000 description 1
• 239000002360 explosive Substances 0.000 description 1
• 238000000605 extraction Methods 0.000 description 1
• 238000001125 extrusion Methods 0.000 description 1
• 230000002600 fibrillogenic effect Effects 0.000 description 1
• MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
• 229910003472 fullerene Inorganic materials 0.000 description 1
• 238000010230 functional analysis Methods 0.000 description 1
• 125000000524 functional group Chemical group 0.000 description 1
• 102000037865 fusion proteins Human genes 0.000 description 1
• 108020001507 fusion proteins Proteins 0.000 description 1
• 229940044627 gamma-interferon Drugs 0.000 description 1
• 238000007429 general method Methods 0.000 description 1
• 230000002068 genetic effect Effects 0.000 description 1
• 229940116332 glucose oxidase Drugs 0.000 description 1
• 235000019420 glucose oxidase Nutrition 0.000 description 1
• 235000013922 glutamic acid Nutrition 0.000 description 1
• 239000004220 glutamic acid Substances 0.000 description 1
• ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
• ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
• 230000036541 health Effects 0.000 description 1
• 238000010438 heat treatment Methods 0.000 description 1
• 210000002443 helper t lymphocyte Anatomy 0.000 description 1
• 206010073071 hepatocellular carcinoma Diseases 0.000 description 1
• 231100000844 hepatocellular carcinoma Toxicity 0.000 description 1
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
• 125000000487 histidyl group Chemical class [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 1
• 108010028295 histidylhistidine Proteins 0.000 description 1
• 239000000710 homodimer Substances 0.000 description 1
• 239000005556 hormone Substances 0.000 description 1
• 229940088597 hormone Drugs 0.000 description 1
• 208000010544 human prion disease Diseases 0.000 description 1
• 230000008348 humoral response Effects 0.000 description 1
• 150000003840 hydrochlorides Chemical class 0.000 description 1
• 239000000017 hydrogel Substances 0.000 description 1
• 125000004356 hydroxy functional group Chemical group O* 0.000 description 1
• 125000002768 hydroxyalkyl group Chemical group 0.000 description 1
• 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
• 125000004464 hydroxyphenyl group Chemical group 0.000 description 1
• 229960002591 hydroxyproline Drugs 0.000 description 1
• 125000001841 imino group Chemical group [H]N=* 0.000 description 1
• 230000008105 immune reaction Effects 0.000 description 1
• 230000000984 immunochemical effect Effects 0.000 description 1
• 230000005847 immunogenicity Effects 0.000 description 1
• 230000017555 immunoglobulin mediated immune response Effects 0.000 description 1
• 230000016784 immunoglobulin production Effects 0.000 description 1
• 230000004957 immunoregulator effect Effects 0.000 description 1
• 229960001438 immunostimulant agent Drugs 0.000 description 1
• 239000003022 immunostimulating agent Substances 0.000 description 1
• 238000009169 immunotherapy Methods 0.000 description 1
• 230000001976 improved effect Effects 0.000 description 1
• 230000006872 improvement Effects 0.000 description 1
• 238000011065 in-situ storage Methods 0.000 description 1
• 238000010348 incorporation Methods 0.000 description 1
• 230000006698 induction Effects 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 229910052500 inorganic mineral Inorganic materials 0.000 description 1
• 230000010354 integration Effects 0.000 description 1
• 230000002452 interceptive effect Effects 0.000 description 1
• 229940079322 interferon Drugs 0.000 description 1
• 229940047124 interferons Drugs 0.000 description 1
• 229940047122 interleukins Drugs 0.000 description 1
• 230000000968 intestinal effect Effects 0.000 description 1
• 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
• NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
• 210000003292 kidney cell Anatomy 0.000 description 1
• HXEACLLIILLPRG-RXMQYKEDSA-N l-pipecolic acid Natural products OC(=O)[C@H]1CCCCN1 HXEACLLIILLPRG-RXMQYKEDSA-N 0.000 description 1
• 238000002372 labelling Methods 0.000 description 1
• JJTUDXZGHPGLLC-UHFFFAOYSA-N lactide Chemical compound CC1OC(=O)C(C)OC1=O JJTUDXZGHPGLLC-UHFFFAOYSA-N 0.000 description 1
• 230000003902 lesion Effects 0.000 description 1
• 210000004558 lewy body Anatomy 0.000 description 1
• 239000006193 liquid solution Substances 0.000 description 1
• 239000006194 liquid suspension Substances 0.000 description 1
• 210000004185 liver Anatomy 0.000 description 1
• 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
• 229960000274 lysozyme Drugs 0.000 description 1
• 239000004325 lysozyme Substances 0.000 description 1
• 235000010335 lysozyme Nutrition 0.000 description 1
• 230000002101 lytic effect Effects 0.000 description 1
• 150000002678 macrocyclic compounds Chemical class 0.000 description 1
• 210000002540 macrophage Anatomy 0.000 description 1
• HCWCAKKEBCNQJP-UHFFFAOYSA-N magnesium orthosilicate Chemical compound [Mg+2].[Mg+2].[O-][Si]([O-])([O-])[O-] HCWCAKKEBCNQJP-UHFFFAOYSA-N 0.000 description 1
• 239000000391 magnesium silicate Substances 0.000 description 1
• 229910052919 magnesium silicate Inorganic materials 0.000 description 1
• 235000019792 magnesium silicate Nutrition 0.000 description 1
• 230000014759 maintenance of location Effects 0.000 description 1
• 206010025482 malaise Diseases 0.000 description 1
• 150000002690 malonic acid derivatives Chemical class 0.000 description 1
• 125000000311 mannosyl group Chemical group C1([C@@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
• 238000004949 mass spectrometry Methods 0.000 description 1
• 235000013372 meat Nutrition 0.000 description 1
• 208000023356 medullary thyroid gland carcinoma Diseases 0.000 description 1
• VDXZNPDIRNWWCW-UHFFFAOYSA-N melitten Chemical group NCC(=O)NC(C(C)CC)C(=O)NCC(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CC(C)C)C(=O)NC(CCCCN)C(=O)NC(C(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)O)C(=O)NC(C(C)O)C(=O)NCC(=O)NC(CC(C)C)C(=O)N1CCCC1C(=O)NC(C)C(=O)NC(CC(C)C)C(=O)NC(C(C)CC)C(=O)NC(CO)C(=O)NC(C(=O)NC(C(C)CC)C(=O)NC(CCCCN)C(=O)NC(CCCNC(N)=N)C(=O)NC(CCCCN)C(=O)NC(CCCNC(N)=N)C(=O)NC(CCC(N)=O)C(=O)NC(CCC(N)=O)C(N)=O)CC1=CNC2=CC=CC=C12 VDXZNPDIRNWWCW-UHFFFAOYSA-N 0.000 description 1
• 238000002844 melting Methods 0.000 description 1
• 230000008018 melting Effects 0.000 description 1
• 244000005700 microbiome Species 0.000 description 1
• JMUHBNWAORSSBD-WKYWBUFDSA-N mifamurtide Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@@H](OC(=O)CCCCCCCCCCCCCCC)COP(O)(=O)OCCNC(=O)[C@H](C)NC(=O)CC[C@H](C(N)=O)NC(=O)[C@H](C)NC(=O)[C@@H](C)O[C@H]1[C@H](O)[C@@H](CO)OC(O)[C@@H]1NC(C)=O JMUHBNWAORSSBD-WKYWBUFDSA-N 0.000 description 1
• 229960005225 mifamurtide Drugs 0.000 description 1
• 230000003278 mimic effect Effects 0.000 description 1
• 239000011707 mineral Substances 0.000 description 1
• 238000002156 mixing Methods 0.000 description 1
• 238000001823 molecular biology technique Methods 0.000 description 1
• 238000010369 molecular cloning Methods 0.000 description 1
• 238000000302 molecular modelling Methods 0.000 description 1
• 238000012544 monitoring process Methods 0.000 description 1
• 229940035032 monophosphoryl lipid a Drugs 0.000 description 1
• 125000001446 muramyl group Chemical group N[C@@H](C=O)[C@@H](O[C@@H](C(=O)*)C)[C@H](O)[C@H](O)CO 0.000 description 1
• 201000000050 myeloid neoplasm Diseases 0.000 description 1
• JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 description 1
• 239000007923 nasal drop Substances 0.000 description 1
• 229940100662 nasal drops Drugs 0.000 description 1
• 229940100652 nasal gel Drugs 0.000 description 1
• 229940052404 nasal powder Drugs 0.000 description 1
• 229940097496 nasal spray Drugs 0.000 description 1
• 239000007922 nasal spray Substances 0.000 description 1
• 210000005044 neurofilament Anatomy 0.000 description 1
• 230000007935 neutral effect Effects 0.000 description 1
• 230000003472 neutralizing effect Effects 0.000 description 1
• 230000009871 nonspecific binding Effects 0.000 description 1
• 231100000252 nontoxic Toxicity 0.000 description 1
• 230000003000 nontoxic effect Effects 0.000 description 1
• 238000007899 nucleic acid hybridization Methods 0.000 description 1
• 239000007764 o/w emulsion Substances 0.000 description 1
• 239000003921 oil Substances 0.000 description 1
• 239000002751 oligonucleotide probe Substances 0.000 description 1
• 231100000590 oncogenic Toxicity 0.000 description 1
• 230000002246 oncogenic effect Effects 0.000 description 1
• 238000011275 oncology therapy Methods 0.000 description 1
• 230000003287 optical effect Effects 0.000 description 1
• 150000007524 organic acids Chemical class 0.000 description 1
• 235000005985 organic acids Nutrition 0.000 description 1
• 239000003960 organic solvent Substances 0.000 description 1
• 229960003104 ornithine Drugs 0.000 description 1
• 230000003204 osmotic effect Effects 0.000 description 1
• 210000001672 ovary Anatomy 0.000 description 1
• 239000006179 pH buffering agent Substances 0.000 description 1
• 238000002638 palliative care Methods 0.000 description 1
• 229940055729 papain Drugs 0.000 description 1
• 235000019834 papain Nutrition 0.000 description 1
• 230000008506 pathogenesis Effects 0.000 description 1
• 108091000699 pea lectin Proteins 0.000 description 1
• 229960001639 penicillamine Drugs 0.000 description 1
• XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
• 229940111202 pepsin Drugs 0.000 description 1
• 239000000546 pharmaceutical excipient Substances 0.000 description 1
• 230000000144 pharmacologic effect Effects 0.000 description 1
• NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
• 235000021317 phosphate Nutrition 0.000 description 1
• DCWXELXMIBXGTH-QMMMGPOBSA-N phosphonotyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-QMMMGPOBSA-N 0.000 description 1
• 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
• 230000026731 phosphorylation Effects 0.000 description 1
• 238000006366 phosphorylation reaction Methods 0.000 description 1
• DCWXELXMIBXGTH-UHFFFAOYSA-N phosphotyrosine Chemical compound OC(=O)C(N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-UHFFFAOYSA-N 0.000 description 1
• 230000000704 physical effect Effects 0.000 description 1
• HXEACLLIILLPRG-UHFFFAOYSA-N pipecolic acid Chemical compound OC(=O)C1CCCCN1 HXEACLLIILLPRG-UHFFFAOYSA-N 0.000 description 1
• 210000002826 placenta Anatomy 0.000 description 1
• 229920000724 poly(L-arginine) polymer Polymers 0.000 description 1
• 229920001606 poly(lactic acid-co-glycolic acid) Polymers 0.000 description 1
• 229940065514 poly(lactide) Drugs 0.000 description 1
• 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
• 239000002745 poly(ortho ester) Substances 0.000 description 1
• 229920000553 poly(phenylenevinylene) Polymers 0.000 description 1
• 229920002492 poly(sulfone) Polymers 0.000 description 1
• 229920000058 polyacrylate Polymers 0.000 description 1
• 230000008488 polyadenylation Effects 0.000 description 1
• 108010011110 polyarginine Proteins 0.000 description 1
• 229920001610 polycaprolactone Polymers 0.000 description 1
• 239000004632 polycaprolactone Substances 0.000 description 1
• 229920000015 polydiacetylene Polymers 0.000 description 1
• 229920000647 polyepoxide Polymers 0.000 description 1
• 229920000570 polyether Polymers 0.000 description 1
• 229920000573 polyethylene Polymers 0.000 description 1
• 229920001721 polyimide Polymers 0.000 description 1
• 229920000656 polylysine Polymers 0.000 description 1
• 230000007824 polyneuropathy Effects 0.000 description 1
• 108010055896 polyornithine Proteins 0.000 description 1
• 229920002714 polyornithine Polymers 0.000 description 1
• 239000001205 polyphosphate Substances 0.000 description 1
• 235000011176 polyphosphates Nutrition 0.000 description 1
• 229920000128 polypyrrole Polymers 0.000 description 1
• 229920000123 polythiophene Polymers 0.000 description 1
• 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
• 235000015277 pork Nutrition 0.000 description 1
• 230000001323 posttranslational effect Effects 0.000 description 1
• 230000003389 potentiating effect Effects 0.000 description 1
• 239000000843 powder Substances 0.000 description 1
• 238000002203 pretreatment Methods 0.000 description 1
• 208000022256 primary systemic amyloidosis Diseases 0.000 description 1
• CWCXERYKLSEGEZ-KDKHKZEGSA-N procalcitonin Chemical compound C([C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)NCC(O)=O)[C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCSC)NC(=O)[C@H]1NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@@H](N)CSSC1)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 CWCXERYKLSEGEZ-KDKHKZEGSA-N 0.000 description 1
• 230000035755 proliferation Effects 0.000 description 1
• 230000001681 protective effect Effects 0.000 description 1
• 230000017854 proteolysis Effects 0.000 description 1
• 230000006337 proteolytic cleavage Effects 0.000 description 1
• 238000010379 pull-down assay Methods 0.000 description 1
• 230000002685 pulmonary effect Effects 0.000 description 1
• 239000000376 reactant Substances 0.000 description 1
• 230000009257 reactivity Effects 0.000 description 1
• 238000009877 rendering Methods 0.000 description 1
• 230000010076 replication Effects 0.000 description 1
• 238000011160 research Methods 0.000 description 1
• 238000002165 resonance energy transfer Methods 0.000 description 1
• 108091008146 restriction endonucleases Proteins 0.000 description 1
• 238000005096 rolling process Methods 0.000 description 1
• 229940043230 sarcosine Drugs 0.000 description 1
• 238000005204 segregation Methods 0.000 description 1
• 239000006152 selective media Substances 0.000 description 1
• 239000004054 semiconductor nanocrystal Substances 0.000 description 1
• 230000035945 sensitivity Effects 0.000 description 1
• 230000035939 shock Effects 0.000 description 1
• 239000000741 silica gel Substances 0.000 description 1
• 229910002027 silica gel Inorganic materials 0.000 description 1
• 239000002002 slurry Substances 0.000 description 1
• 238000002764 solid phase assay Methods 0.000 description 1
• 230000003381 solubilizing effect Effects 0.000 description 1
• 239000002904 solvent Substances 0.000 description 1
• 238000000527 sonication Methods 0.000 description 1
• 238000004611 spectroscopical analysis Methods 0.000 description 1
• 229940063673 spermidine Drugs 0.000 description 1
• 229940063675 spermine Drugs 0.000 description 1
• 229940032094 squalane Drugs 0.000 description 1
• 239000007858 starting material Substances 0.000 description 1
• 150000003432 sterols Chemical class 0.000 description 1
• 235000003702 sterols Nutrition 0.000 description 1
• 238000007920 subcutaneous administration Methods 0.000 description 1
• 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
• 229920001059 synthetic polymer Polymers 0.000 description 1
• 239000000454 talc Substances 0.000 description 1
• 229910052623 talc Inorganic materials 0.000 description 1
• 210000001138 tear Anatomy 0.000 description 1
• 229960000814 tetanus toxoid Drugs 0.000 description 1
• 238000002560 therapeutic procedure Methods 0.000 description 1
• 230000004797 therapeutic response Effects 0.000 description 1
• 229940104230 thymidine Drugs 0.000 description 1
• 229960002175 thyroglobulin Drugs 0.000 description 1
• 231100000419 toxicity Toxicity 0.000 description 1
• 230000001988 toxicity Effects 0.000 description 1
• 239000003053 toxin Substances 0.000 description 1
• 231100000765 toxin Toxicity 0.000 description 1
• FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
• 238000000844 transformation Methods 0.000 description 1
• 230000001052 transient effect Effects 0.000 description 1
• 230000010474 transient expression Effects 0.000 description 1
• 230000014621 translational initiation Effects 0.000 description 1
• 230000005945 translocation Effects 0.000 description 1
• 208000037956 transmissible mink encephalopathy Diseases 0.000 description 1
• 238000002054 transplantation Methods 0.000 description 1
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
• 239000013638 trimer Substances 0.000 description 1
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
• JOPDZQBPOWAEHC-UHFFFAOYSA-H tristrontium;diphosphate Chemical compound [Sr+2].[Sr+2].[Sr+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O JOPDZQBPOWAEHC-UHFFFAOYSA-H 0.000 description 1
• 239000012588 trypsin Substances 0.000 description 1
• 102000003390 tumor necrosis factor Human genes 0.000 description 1
• 238000002525 ultrasonication Methods 0.000 description 1
• 241001430294 unidentified retrovirus Species 0.000 description 1
• 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
• 229920002554 vinyl polymer Polymers 0.000 description 1
• 239000011534 wash buffer Substances 0.000 description 1
• 238000005406 washing Methods 0.000 description 1
• 238000005303 weighing Methods 0.000 description 1
• 239000000080 wetting agent Substances 0.000 description 1
• 238000002424 x-ray crystallography Methods 0.000 description 1
• PAPBSGBWRJIAAV-UHFFFAOYSA-N ε-Caprolactone Chemical compound O=C1CCCCCO1 PAPBSGBWRJIAAV-UHFFFAOYSA-N 0.000 description 1