NO324041B1 - Fremgangsmate for ekstrahering av DNA, samt fremgangsmate for deteksjon av en DNA-inneholdende mikroorganisme i serum eller plasma. - Google Patents
Fremgangsmate for ekstrahering av DNA, samt fremgangsmate for deteksjon av en DNA-inneholdende mikroorganisme i serum eller plasma. Download PDFInfo
- Publication number
- NO324041B1 NO324041B1 NO20000539A NO20000539A NO324041B1 NO 324041 B1 NO324041 B1 NO 324041B1 NO 20000539 A NO20000539 A NO 20000539A NO 20000539 A NO20000539 A NO 20000539A NO 324041 B1 NO324041 B1 NO 324041B1
- Authority
- NO
- Norway
- Prior art keywords
- plasma
- serum
- dna
- approx
- alkalinized
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 90
- 210000002966 serum Anatomy 0.000 title claims abstract description 77
- 244000005700 microbiome Species 0.000 title claims abstract description 17
- 239000006228 supernatant Substances 0.000 claims abstract description 22
- 239000003513 alkali Substances 0.000 claims abstract description 17
- 238000010438 heat treatment Methods 0.000 claims abstract description 12
- 230000003321 amplification Effects 0.000 claims description 25
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 25
- 238000001514 detection method Methods 0.000 claims description 19
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 17
- 239000000047 product Substances 0.000 claims description 15
- 238000005119 centrifugation Methods 0.000 claims description 8
- 241000701024 Human betaherpesvirus 5 Species 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 3
- 239000003155 DNA primer Substances 0.000 claims description 3
- 241000700605 Viruses Species 0.000 claims description 3
- 108020004414 DNA Proteins 0.000 description 54
- 238000003752 polymerase chain reaction Methods 0.000 description 30
- 229920000642 polymer Polymers 0.000 description 29
- 239000000523 sample Substances 0.000 description 27
- 239000011324 bead Substances 0.000 description 17
- 239000011541 reaction mixture Substances 0.000 description 14
- 210000000265 leukocyte Anatomy 0.000 description 10
- 239000013612 plasmid Substances 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 239000003112 inhibitor Substances 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 238000012408 PCR amplification Methods 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 238000000605 extraction Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000007400 DNA extraction Methods 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 5
- 150000007523 nucleic acids Chemical class 0.000 description 5
- 239000013615 primer Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000005464 sample preparation method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000011109 contamination Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 239000013610 patient sample Substances 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 3
- 230000002452 interceptive effect Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- NKDFYOWSKOHCCO-YPVLXUMRSA-N 20-hydroxyecdysone Chemical compound C1[C@@H](O)[C@@H](O)C[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@@](C)(O)[C@H](O)CCC(C)(O)C)CC[C@]33O)C)C3=CC(=O)[C@@H]21 NKDFYOWSKOHCCO-YPVLXUMRSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108060003393 Granulin Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 238000002944 PCR assay Methods 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 108091008048 CMVpp65 Proteins 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 241000450599 DNA viruses Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000700584 Simplexvirus Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 206010058874 Viraemia Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001739 density measurement Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000013101 initial test Methods 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11849699P | 1999-02-03 | 1999-02-03 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO20000539D0 NO20000539D0 (no) | 2000-02-02 |
| NO20000539L NO20000539L (no) | 2000-08-04 |
| NO324041B1 true NO324041B1 (no) | 2007-07-30 |
Family
ID=22378966
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO20000539A NO324041B1 (no) | 1999-02-03 | 2000-02-02 | Fremgangsmate for ekstrahering av DNA, samt fremgangsmate for deteksjon av en DNA-inneholdende mikroorganisme i serum eller plasma. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US6268127B1 (zh) |
| EP (1) | EP1026241B1 (zh) |
| JP (1) | JP4468534B2 (zh) |
| KR (1) | KR100845949B1 (zh) |
| CN (1) | CN100334102C (zh) |
| AT (1) | ATE267251T1 (zh) |
| AU (1) | AU769709C (zh) |
| CA (1) | CA2297547C (zh) |
| DE (1) | DE60010776T2 (zh) |
| DK (1) | DK1026241T3 (zh) |
| ES (1) | ES2220340T3 (zh) |
| NO (1) | NO324041B1 (zh) |
| PT (1) | PT1026241E (zh) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020028386A (ko) * | 2000-10-09 | 2002-04-17 | 박제철 | 소량의 동물조직으로부터 미토콘드리아 디.엔.에이의순수분리 방법 |
| WO2004033665A2 (en) * | 2002-10-11 | 2004-04-22 | Imutex Pharmaceuticals, Inc. | Heterologous plasma cocktail for hiv treatment |
| WO2016187780A1 (en) * | 2015-05-25 | 2016-12-01 | Coyote Bioscience Yixing Co., Ltd. | Device and method for sample collection |
| CN107683330A (zh) * | 2015-05-25 | 2018-02-09 | 卡尤迪生物科技（北京）有限公司 | 用于样品收集的装置和方法 |
| CN108795926A (zh) * | 2018-06-27 | 2018-11-13 | 中国农业科学院麻类研究所 | 一种dna模板快速制备方法 |
| JP7329912B2 (ja) * | 2018-10-09 | 2023-08-21 | 公益財団法人筑波メディカルセンター | 微生物の検出方法 |
| CN112805388A (zh) * | 2018-10-09 | 2021-05-14 | 公益财团法人筑波医疗中心 | 微生物的检测方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5229297A (en) | 1989-02-03 | 1993-07-20 | Eastman Kodak Company | Containment cuvette for PCR and method of use |
| US5089233A (en) | 1989-06-12 | 1992-02-18 | Eastman Kodak Company | Processing apparatus for a chemical reaction pack |
| US5380489A (en) | 1992-02-18 | 1995-01-10 | Eastman Kodak Company | Element and method for nucleic acid amplification and detection using adhered probes |
| KR950701979A (ko) | 1992-06-08 | 1995-05-17 | 다니엘 엘. 캐시앙 | 단핵 세포로부터 핵산의 조제 방법(Preparation of Nucleic Acid from Mononuclear Cells) |
| US5582988A (en) | 1994-09-15 | 1996-12-10 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same |
| AU2324997A (en) * | 1996-03-15 | 1997-10-01 | Penn State Research Foundation, The | Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays |
-
2000
- 2000-01-28 US US09/493,352 patent/US6268127B1/en not_active Expired - Lifetime
- 2000-02-01 DK DK00300762T patent/DK1026241T3/da active
- 2000-02-01 CA CA002297547A patent/CA2297547C/en not_active Expired - Fee Related
- 2000-02-01 ES ES00300762T patent/ES2220340T3/es not_active Expired - Lifetime
- 2000-02-01 AT AT00300762T patent/ATE267251T1/de active
- 2000-02-01 PT PT00300762T patent/PT1026241E/pt unknown
- 2000-02-01 DE DE60010776T patent/DE60010776T2/de not_active Expired - Lifetime
- 2000-02-01 EP EP00300762A patent/EP1026241B1/en not_active Expired - Lifetime
- 2000-02-02 AU AU14880/00A patent/AU769709C/en not_active Ceased
- 2000-02-02 KR KR1020000005130A patent/KR100845949B1/ko not_active IP Right Cessation
- 2000-02-02 NO NO20000539A patent/NO324041B1/no not_active IP Right Cessation
- 2000-02-03 CN CNB001046985A patent/CN100334102C/zh not_active Expired - Fee Related
- 2000-02-03 JP JP2000032644A patent/JP4468534B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1026241B1 (en) | 2004-05-19 |
| JP4468534B2 (ja) | 2010-05-26 |
| AU769709B2 (en) | 2004-01-29 |
| CN100334102C (zh) | 2007-08-29 |
| AU769709C (en) | 2004-10-14 |
| CA2297547A1 (en) | 2000-08-03 |
| ATE267251T1 (de) | 2004-06-15 |
| JP2000279199A (ja) | 2000-10-10 |
| DK1026241T3 (da) | 2004-08-30 |
| KR100845949B1 (ko) | 2008-07-11 |
| KR20000076596A (ko) | 2000-12-26 |
| CN1270962A (zh) | 2000-10-25 |
| PT1026241E (pt) | 2004-09-30 |
| US6268127B1 (en) | 2001-07-31 |
| DE60010776D1 (de) | 2004-06-24 |
| NO20000539L (no) | 2000-08-04 |
| AU1488000A (en) | 2000-08-10 |
| EP1026241A1 (en) | 2000-08-09 |
| NO20000539D0 (no) | 2000-02-02 |
| CA2297547C (en) | 2006-12-19 |
| DE60010776T2 (de) | 2005-05-12 |
| ES2220340T3 (es) | 2004-12-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM1K | Lapsed by not paying the annual fees |