NL2025521A - Method for promoting seedling strengthening by physical stimulation of cucumber cotyledon - Google Patents

Method for promoting seedling strengthening by physical stimulation of cucumber cotyledon Download PDF

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Publication number
NL2025521A
NL2025521A NL2025521A NL2025521A NL2025521A NL 2025521 A NL2025521 A NL 2025521A NL 2025521 A NL2025521 A NL 2025521A NL 2025521 A NL2025521 A NL 2025521A NL 2025521 A NL2025521 A NL 2025521A
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cotyledon
cucumber
physical
stimulation
leaf
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NL2025521A
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Dutch (nl)
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NL2025521B1 (en
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Yang Ning
Wen Dan
Sun Kaining
Wang Ke'an
Wang Xiao
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Shandong Acad Of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention discloses a method for promoting seedling strengthening by physical cotyledon stimulation of cucumber and belongs to the technical field of cucumber seedling growing. The method for promoting seedling strengthening in the present invention includes the following steps: performing physical cotyledon stimulation when the cotyledons coming out of shoot tip flattens developed, wherein the physical stimulation includes an operation of performing physical disturbance at an optional position of unilateral cotyledon or removing partial cotyledon within 1/2 of leaf vein length of a single unilateral cotyledon perpendicular to the main leaf vein of the cotyledon; placing cucumber seedlings on a cool lee side until the wound heals after physical stimulation; and continuing to cultivate the cucumber seedlings after the wound heals. According to the present invention, by removing the partial cotyledon when the cucumber cotyledon develop as physical stimulation, endogenous hormone production is facilitated, and root and seedling strengthening is promoted; and problems such as toxication and pollution caused by exogenous hormone application in a cucumber seedling growing process are solved. The method is green and environment-friendly, and cost is saved. The cucumber seedlings cultivated by the method in the present invention are better than conventional seedling growing in the aspects of biomass, root activity and seedling strength index. The present invention is simple in operation, easy to master and suitable for plug seedling growing of vegetables.

Description

METHOD FOR PROMOTING SEEDLING STRENGTHENING BY PHYSICAL STIMULATION
OF CUCUMBER COTYLEDON Technical Field The present invention belongs to the technical field of cucumber seedling growing, and particularly relates to a method for promoting seedling strengthening by physical cotyledon stimulation of cucumber.
Background Cucumber is an important vegetable crop. The scale and yield of cucumber in China rank first in the world. The cucumber plays an important role in structural adjustment of agriculture and increase of farmer income. Plug seedling growing of cucumber may achieve advantages of increasing seedling growing efficiency, saving seeds and space, bringing convenience to management, facilitating pest control and performing large-scale production, and is widely applied to modern facility agriculture.
For the traditional plug seedling growing technology, seedlings are mainly strengthened by spraying chlormequat chloride, mepiquat chloride, B9 and the like in production, or hormones are directly applied to nursery substrates, thereby causing hormone poisoning of seedlings, and harm of residual poisons to soil, environment and next-stubble crops. To overcome the above defects, a green and safe seedling strengthening method is in an urgent need.
Summary To overcome defects of the prior art, the present invention provides a method for promoting seedling strengthening by physical cotyledon stimulation of cucumber. According to the present invention, cucumber seedlings are strengthened in a manner of performing physical cotyledon stimulation on the cucumber, and cultivated cucumber seedlings are better than conventional seedlings growing in the aspects of biomass, root activity and seedling strength index.
Technical solutions of the present invention are as follows: The method for promoting seedling strengthening by physical cotyledon stimulation of cucumber includes the following steps: performing physical cotyledon stimulation after induced germination when the cotyledons coming out of the shoot tip developed, i.e. after the cotyledons coming out of the shoot tip flattened, wherein the physical stimulation includes an operation of performing physical disturbance at an optional position of unilateral cotyledon or removing partial cotyledon within 1/2 of leaf vein length of a single unilateral cotyledon perpendicular to the main leaf vein of the cotyledon; placing cucumber seedlings on a cool lee side until the wound heals after physical stimulation; and continuing to cultivate the cucumber seedlings after the wound heals.
As a preferred solution, when the cotyledon coming out of the shoot tip developed, partial cotyledon within 1/8-3/8 of leaf vein length of a single unilateral cotyledon is removed perpendicular to the main leaf vein of the cotyledon.
Further, when the cotyledon coming out of the shoot tip developed, partial cotyledon within 1/4 of leaf vein length of a single unilateral cotyledon is removed perpendicular to the main leaf vein of the cotyledon.
As a preferred solution, after physical stimulation, the cucumber seedlings are placed on the cool lee side for 1-2 hours.
As a preferred solution, the seedling growing environment is as follows: a light period is 12h/12h, a diurnal temperature is 28°C/18°C, daytime light intensity is 600 umol-m2-s! (where 18 pumol-m2-s' = 1000 Ix), and air humidity is 55-65%; and the seedlings are watered with clear water and nutrient solution before two leaves and one bud develop, and the seedlings with cotyledons are watered with total nutrient solution after two leaves and one core are developed until seedling growing is ended.
As a preferred solution, after induced germination, the cucumber is seeded in an empty plug filled with nursery substrate.
Further, the nursery substrate is a substrate prepared by mixing turf, vermiculite and perlite according to a volume ratio of 3:1:1.
The present invention has beneficial effects as follows:
1. According to the present invention, by removing the partial cotyledon in the flattening period of the cucumber cotyledon to perform physical stimulation, endogenous hormone production is facilitated, and root and seedling strengthening is promoted; and problems such as toxication and pollution caused by exogenous hormone application in the cucumber seedling growing process are solved; the method is green and environment-friendly, and cost is saved.
2. The cucumber seedlings cultivated by the method in the present invention are better than conventional seedling growing in the aspects of the biomass, root activity and seedling strength index.
3. The method in the present invention is simple in operation, easy to master and suitable for plug seedling growing of vegetables.
Description of Drawings To clearly illustrate technical solutions in embodiments of the present invention or in the prior art, drawings to be used in the description of embodiments or the prior art are simply introduced below. Apparently, the drawings in the description below are some embodiments of the present invention only. Other drawings may be obtained by those ordinary skilled in the art according to the drawings on premise of not making creative work.
Fig. 1 is a diagram of influence of different degrees of physical cotyledon stimulation degrees on plants.
Detailed Description A method for promoting seedling strengthening by physical cotyledon stimulation of cucumber includes the following steps: performing physical cotyledon stimulation after induced germination when the cotyledons coming out of shoot tip developed wherein the physical stimulation includes an operation of performing physical disturbance at an optional position of unilateral cotyledon or removing partial cotyledon within 1/2 of leaf vein length of a single unilateral cotyledon perpendicular to the main leaf vein of the cotyledon; placing cucumber seedlings on a coal lee side until the wound heals after physical stimulation; and continuing to cultivate the cucumber seedlings after the wound heals.
The physical disturbance is performed at an optional position of the unilateral cotyledon, wherein the physical disturbance includes an operation of filing a hole or cutting one or more seams on the unilateral cotyledon; the area of the hole is smaller than 1/2 of the area of the whole cotyledon; the operation of cutting one or more seams is that the cotyledon does not drop from the cutting position before the main leaf vein is cut.
As a preferred solution, when the cotyledon coming out of the shoot tip developed, partial cotyledon within 1/8-3/8 of leaf vein length of a single unilateral cotyledon is removed perpendicular to the main leaf vein of the cotyledon.
Further, when the cotyledon coming out of the shoot tip developed, partial cotyledon within 1/4 of leaf vein length of a single unilateral cotyledon is removed perpendicular to the main leaf vein of the cotyledon.
As a preferred solution, after physical stimulation, the cucumber seedlings are placed on the cool lee side for 1-2 hours.
As a preferred solution, the seedling growing environment is as follows: a light period is 12h/12h, a diurnal temperature is 28°C/18°C, daytime light intensity is 600 umol-m™-s™*, and air humidity is 55-65%; and the seedling is watered with clear water and nutrient solution before to two leaves and one bud develop, and the seeding with cotyledons are watered with total nutrient solution after two leaves and one bud developed until seedling growing is ended.
As a preferred solution, after induced germination, the cucumber is seeded in an empty plug filled with nursery substrate.
Further, the nursery substrate is a substrate prepared by mixing turf, vermiculite and perlite according to a volume ratio of 3:1:1 Application examples: 1) Accelerating germination and seeding growth to cotyledon flattening including steps: selecting full seeds, soaking the seeds in warm water at about 50°C, taking out the seeds within 6 h, sucking surface moisture, wrapping the seeds by wet gauze, performing germination induction at 28°C, and seeding the seeds in a 50-well plug filled with a nursery substrate (a ratio of turf to vermiculite to perlite is 3:1:1) after the seeds become white.
A cultivation environment is as follows: a light period is 12h/12h, a diurnal temperature is 28°C/18°C, daytime light intensity is 600 umol-m-s™, and air humidity is 60%. After 7 days from accelerating germination seeding of cucumber, physical stimulation may be performed when the cotyledon coming out of the shoot tip flattens. 2) Physical stimulation of different degrees Fig. 1 respectively shows plant growth situations in the same cultivation environment from the left to right for a cucumber seedling of normal growth without any treatment, a cucumber seedling subjected to mechanical stimulation of a unilateral cotyledon in the cotyledon flattening period (a seam of not longer than one half of the cotyledon width is cut on the unilateral cotyledon), a cucumber seedling of which 1/4 of leaf vein length of a single unilateral cotyledon is removed perpendicular to the main leaf vein of the cotyledon in the cotyledon flattening period, a cucumber seedling of which 1/2 of the leaf vein length of the single unilateral cotyledon is removed perpendicular to the main leaf vein of the cotyledon in the cotyledon flattening period, a cucumber seedling of which 3/4 of the leaf vein length of the single unilateral cotyledon is removed perpendicular to the main leaf vein of the cotyledon in the cotyledon flattening period, and a cucumber seedling of which the single unilateral cotyledon is totally removed in the cotyledon flattening period.
Treatment to cucumber seedlings after physical stimulation: including steps: placing the cucumber seedlings on a cool lee side after physical stimulation; and continuing to cultivate the cucumber seedlings in a normal seedling growing environment after the wound heals (about 1 hour), wherein the light period is 12h/12h, the diurnal temperature is 28°C/18°C, the daytime light intensity is 600 ymol-m2-s!, and the air humidity is 60%. The cotyledon is watered with clear water before flattening, watered with 1/2 nutrient solution while flattening to two leaves and one core and watered with total nutrient solution after flattening to two leaves and one core.
The seedling can be transplanted for field planting while growing to four leaves and one core.
It may be seen from Fig. 1, compared with CK control, by virtue of mechanical stimulation of T1 unilateral cotyledon (a seam not longer than one half of the cotyledon width is cut on the unilateral cotyledon) and removal of 1/4 of T2 unilateral cotyledon, growth of plants can be improved, the leaf area of the plants is increased, and growth is stronger.
Moreover, the effect is the best when 1/4 of the unilateral cotyledon is removed.
Compared with CK, removal of 1/2 of T3 unilateral cotyledon does not have great change.
In addition, overstimulation of T4 and T5 (removal of more than 1/2 of the leaf vein length of the single unilateral cotyledon) may cause plant growth inhibition, growth vigor of the cucumber seedlings is poorer than that of conventional breeding, and removal of more than 1/2 of the leaf vein length of the single unilateral cotyledon is not recommended.

Claims (7)

CONCLUSIESCONCLUSIONS 1. Een werkwijze voor het bevorderen van de sterkte van zaailingen van komkommer door fysieke kiembladstimulatie, omvattende de volgende stappen: 5 — uitvoeren van fysieke stimulatie van de kiembladeren na induceren van de kieming van het zaad zodra de kiembladeren die uit de scheut komen zijn ontwikkeld, waarbij de fysieke stimulatie omvat het aan één zijde fysiek verstoren bij een willekeurige positie van een kiemblad, of het aan één zijde verwijderen van een deel van het kiemblad binnen de helft van de nerflengte van het kiemblad loodrecht op de hoofdbladnerf van het kiemblad; — plaatsen van de zaailingen van komkommer op een koele windluwe plaats totdat de wond ontstaan na fysieke stimulatie heelt; en — laten groeien van de komkommer nadat de wond is geheeld.A method for promoting the vigor of cucumber seedlings by physical germ leaf stimulation, comprising the following steps: 5 - performing physical stimulation of the germ leaves after inducing the germination of the seed once the germ leaves emerging from the shoot have developed wherein the physical stimulation comprises physically disrupting one side at any position of a seed leaf, or removing on one side a portion of the seed leaf within half the grain length of the seed leaf perpendicular to the main leaf vein of the seed leaf; - placing the cucumber seedlings in a cool, wind-free place until the wound heals after physical stimulation; and - growing the cucumber after the wound has healed. 2. De werkwijze voor het bevorderen van de sterkte van zaailingen van komkommer door fysieke kiembladstimulatie volgens conclusie 1, waarbij zodra de kiembladeren die uit de scheut komen zijn ontwikkeld aan één zijde een deel van de zaadlob binnen 1/8° — 3/8° van de nerflengte loodrecht op de hoofdnerf van het kiemblad wordt verwijderd.The method of promoting cucumber seedling vigor by physical germ leaf stimulation according to claim 1, wherein once the germ leaves emerging from the shoot have developed on one side a portion of the cotyledon within 1/8 ° - 3/8 ° of the grain length perpendicular to the midrib of the germ layer is removed. 3. De werkwijze voor het bevorderen van de sterkte van zaailingen van komkommer door fysieke kiembladstimulatie volgens conclusie 1 of 2, waarbij zodra de kiembladeren die uit de scheut komen zijn ontwikkeld aan één zijde een deel van het kiemblad binnen 1/4° van de nerflengte loodrecht op de hoofdnerf van de zaadlob wordt verwijderd.The method of promoting cucumber seedling vigor by physical germ leaf stimulation according to claim 1 or 2, wherein once the germ leaves emerging from the shoot have developed on one side a portion of the germ leaf within 1/4 of the grain length perpendicular to the midrib of the cotyledon is removed. 4. De werkwijze voor het bevorderen van de sterkte van zaailingen van komkommer door fysieke kiembladstimulatie volgens conclusie 1, waarbij de zaailingen van komkommer na de fysieke stimulatie gedurende 1 — 2 uur op een koele windluwe plaats worden geplaatst.The method of promoting the potency of cucumber seedlings by physical germ leaf stimulation according to claim 1, wherein the cucumber seedlings are placed in a cool, wind-free place for 1 - 2 hours after the physical stimulation. 5. De werkwijze voor het bevorderen van de sterkte van zaailingen van komkommer door fysieke kiembladstimulatie volgens conclusie 1, waarbij de groeiomstandigheden voor het laten groeien van de zaailingen als volgt zijn: — de lichtperiode bedraagt 12uur/12uur, — de temperatuur overdag bedraag 18°C, — de lichtintensiteit overdag bedraagt 600 lux/m?/s, — de luchtvochtigheid 55 — 65 % bedraagt, — de zaailingen zuiver water en een nutriëntenoplossing krijgen voordat twee bladeren en een knop zijn ontwikkeld, en — de zaailingen een complete nutriëntenoplossing krijgen nadat twee bladeren en een knop zijn ontwikkeld totdat het laten groeien van de zaailing wordt beëindigd.The method for promoting the vigor of cucumber seedlings by physical germ leaf stimulation according to claim 1, wherein the growing conditions for growing the seedlings are as follows: the light period is 12h / 12h, the daytime temperature is 18 ° C, - the light intensity during the day is 600 lux / m2 / s, - the humidity is 55 - 65%, - the seedlings receive pure water and a nutrient solution before two leaves and a bud have developed, and - the seedlings receive a complete nutrient solution after two leaves and a bud have developed until the seedling growth is stopped. 6. De werkwijze voor het bevorderen van de sterkte van zaailingen van komkommer door fysieke kiembladstimulatie volgens conclusie 1, waarbij de zaailing van komkommer na induceren van de kieming wordt overgebracht in een lege plug die gevuld is met voedingssubstraat.The method of promoting cucumber seedling vigor by physical seed leaf stimulation according to claim 1, wherein the cucumber seedling is transferred into an empty plug filled with nutrient substrate after inducing germination. 7. De werkwijze voor het bevorderen van de sterkte van zaailingen van komkommer door fysieke kiembladstimulatie volgens conclusie 6, waarbij het voedingssubstraat een substraat dat is bereid door het mengen van turf, vermiculiet en perliet in een volumeverhouding van 3:1: 1.The method of promoting the potency of cucumber seedlings by physical seed leaf stimulation according to claim 6, wherein the nutrient substrate is a substrate prepared by mixing peat, vermiculite and perlite in a volume ratio of 3: 1: 1.
NL2025521A 2019-05-27 2020-05-07 Method for promoting seedling strengthening by physical stimulation of cucumber cotyledon NL2025521B1 (en)

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Citations (2)

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CN109258157A (en) * 2018-11-06 2019-01-25 福建农林大学 A kind of engrafting method that cucumber tip cutting is fixed

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JPH07184476A (en) * 1993-12-27 1995-07-25 Mitsubishi Agricult Mach Co Ltd Seedling cutter in device for grafting
WO2013014585A1 (en) * 2011-07-22 2013-01-31 Basf Plant Science Company Gmbh Plant transformation method
CN105638163A (en) * 2014-11-13 2016-06-08 镇江市五峰茶场 Mechanized tea trimming technology
CN106576959A (en) * 2016-12-21 2017-04-26 贵州省烟草科学研究院 Topping method for flue-cured tobacco
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Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013015442A1 (en) * 2011-07-28 2013-01-31 国立大学法人山口大学 Pathogen-resistant plant body, fruit and leaf stem and induction method thereof, and plant cultivation system
CN109258157A (en) * 2018-11-06 2019-01-25 福建农林大学 A kind of engrafting method that cucumber tip cutting is fixed

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