MXPA06009572A - Metodo para la produccion de acidos grasos poliinsaturados en plantas transgenicas. - Google Patents

Metodo para la produccion de acidos grasos poliinsaturados en plantas transgenicas.

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Publication number
MXPA06009572A
MXPA06009572A MXPA06009572A MXPA06009572A MXPA06009572A MX PA06009572 A MXPA06009572 A MX PA06009572A MX PA06009572 A MXPA06009572 A MX PA06009572A MX PA06009572 A MXPA06009572 A MX PA06009572A MX PA06009572 A MXPA06009572 A MX PA06009572A
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Mexico
Prior art keywords
delta
desaturase
fatty acids
seq
acid
Prior art date
Application number
MXPA06009572A
Other languages
English (en)
Inventor
Petra Cirpus
Guohai Wu
Jorg Bauer
Xiao Qiu
Nagamani Datla
Original Assignee
Basf Plant Science Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
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Priority claimed from DE102004017518A external-priority patent/DE102004017518A1/de
Priority claimed from PCT/EP2004/007957 external-priority patent/WO2005012316A2/de
Priority claimed from DE200410062543 external-priority patent/DE102004062543A1/de
Application filed by Basf Plant Science Gmbh filed Critical Basf Plant Science Gmbh
Priority claimed from PCT/EP2005/001863 external-priority patent/WO2005083093A2/de
Publication of MXPA06009572A publication Critical patent/MXPA06009572A/es

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    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
    • A61K31/202—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having three or more double bonds, e.g. linolenic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
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    • A61P25/00—Drugs for disorders of the nervous system
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    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A61P9/12—Antihypertensives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61Q19/00—Preparations for care of the skin
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/86—Products or compounds obtained by genetic engineering
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    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)

Abstract

La invencion se relaciona a un metodo para producir acidos grasos poliinsaturados en semillas de plantas transgenicas. De acuerdo a tal metodo, los acidos nucleicos, que codifican para polipeptidos con una actividad de ?3-desaturasa, ?12-desaturasa, ?6-desaturasa, ?6-elongasa, ?5-desaturasa, ?5-elongasa y/o ?4-desaturasa, de preferencia para polipeptidos con una actividad de ?-6-desaturasa, ?-6-elongasa y ?-5-desaturasa, se introducen dentro del organismo. Las secuencias de acido nucleico se representan por la SEC. DE IDENT. NO.:11, SEC DE IDENT. NO.: 27, SEC. DE IDENT. NO.: 193, SEC. DE IDENT. NO.: 197, SEC. DE IDENT. NO.: 199 y SEC. DE IDENT. NO.: 201. Ventajosamente, tales secuencias de acido nucleico pueden expresarse en el organismo opcionalmente juntas con otras secuencias de acido nucleico que codifican para polipeptidos de la biosintesis del metabolismo del acido graso o de lipidos. Las secuencias de acido nucleico que codifican para la actividad de ?-6-desaturasa, ?-5-desaturasa, ?-4-desaturasa, ?-12-desaturasa y/o ?-6-elongasa son especialmente ventajosas. Ventajosamente, tales desaturasas y elongasas se originan a partir de thalassiosira, euglena uostreococcus. La invencion se relaciona tambien a un metodo para producir aceites y/o tricacilgliceridos con un contenido incrementado de acidos grasos poliinsaturados de cadena larga. En una modalidad preferida, la invencion se relaciona tambien a un metodo para producir acido araquidonico, acido eicosapentaenoico o acido docosahexaenoico y a un metodo para producir trigliceridos con un contenido incrementado de acidos grasos insaturados, especialmente acido araquidonico, acido eicosapentaenoico y/o acido docosahexaenoico, en plantas transgenicas, de preferencia en semillas de las plantas transgenicas. La invencion se relaciona ademas a la produccion de una planta transgenica con un contenido incrementado de acidos grasos poliinsaturados, especialmente acido araquidonico, acido eicosapentaenoico y/o acido docosahexaenoico, basados en la expresion de las elongasas y desaturasas utilizadas en el metodo inventivo. La invencion se relaciona tambien a moleculas de acido nucleico recombinantes que contienen, juntas o individualmente secuencias de acido nucleico que codifican para los polipeptidos con una actividad de ?-6-desaturasa, ?-6-elongasa, ?-5-desaturasa y ?-5-elongasa, y plantas transgenicas que contienen tales moleculas de acido nucleico recombinante. Otra parte de la invencion se relaciona a aceites, lipidos y/o acidos grasos producidos de acuerdo al metodo inventivo, y al uso del mismo. Ademas, la invencion se relaciona a acidos grasos insaturados y trigliceridos con un contenido incrementado de acidos grasos insaturados, y al uso de los mismos.
MXPA06009572A 2004-02-27 2005-02-23 Metodo para la produccion de acidos grasos poliinsaturados en plantas transgenicas. MXPA06009572A (es)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DE102004009457 2004-02-27
DE102004012370 2004-03-13
DE102004017518A DE102004017518A1 (de) 2004-04-08 2004-04-08 Verfahren zur Herstellung von mehrfach ungesättigten Fettsäuren
DE102004024014 2004-05-14
PCT/EP2004/007957 WO2005012316A2 (de) 2003-08-01 2004-07-16 Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen
DE200410062543 DE102004062543A1 (de) 2004-12-24 2004-12-24 Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Pflanzen
PCT/EP2005/001863 WO2005083093A2 (de) 2004-02-27 2005-02-23 Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen

Publications (1)

Publication Number Publication Date
MXPA06009572A true MXPA06009572A (es) 2007-05-23

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MXPA06009572A MXPA06009572A (es) 2004-02-27 2005-02-23 Metodo para la produccion de acidos grasos poliinsaturados en plantas transgenicas.

Country Status (7)

Country Link
US (2) US9458436B2 (es)
EP (2) EP3543324B1 (es)
JP (1) JP4567047B2 (es)
CN (1) CN1930277B (es)
ES (1) ES2421440T3 (es)
IL (1) IL177231A (es)
MX (1) MXPA06009572A (es)

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US20090222951A1 (en) 2009-09-03
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US20150361404A1 (en) 2015-12-17
EP3543324B1 (de) 2022-11-30
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