WO2005083093A3 - Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen - Google Patents

Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen Download PDF

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WO2005083093A3
WO2005083093A3 PCT/EP2005/001863 EP2005001863W WO2005083093A3 WO 2005083093 A3 WO2005083093 A3 WO 2005083093A3 EP 2005001863 W EP2005001863 W EP 2005001863W WO 2005083093 A3 WO2005083093 A3 WO 2005083093A3
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Prior art keywords
desaturase
fatty acids
seq
acid
relates
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PCT/EP2005/001863
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English (en)
French (fr)
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WO2005083093A2 (de
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Petra Cirpus
Joerg Bauer
Xiao Qiu
Guohai Wu
Nagamani Datla
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Basf Plant Science Gmbh
Petra Cirpus
Joerg Bauer
Xiao Qiu
Guohai Wu
Nagamani Datla
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Priority claimed from DE102004017518A external-priority patent/DE102004017518A1/de
Priority claimed from PCT/EP2004/007957 external-priority patent/WO2005012316A2/de
Priority claimed from DE200410062543 external-priority patent/DE102004062543A1/de
Priority to EP22209519.2A priority Critical patent/EP4219670A3/de
Priority to US10/590,457 priority patent/US9458436B2/en
Priority to EP19155597.8A priority patent/EP3543324B1/de
Priority to PL05715462T priority patent/PL1723220T3/pl
Priority to AU2005217079A priority patent/AU2005217079C1/en
Priority to EP13162907.3A priority patent/EP2623584B1/de
Application filed by Basf Plant Science Gmbh, Petra Cirpus, Joerg Bauer, Xiao Qiu, Guohai Wu, Nagamani Datla filed Critical Basf Plant Science Gmbh
Priority to EP05715462.7A priority patent/EP1723220B1/de
Priority to JP2007500134A priority patent/JP4567047B2/ja
Priority to CA002559360A priority patent/CA2559360A1/en
Priority to RU2006134267/10A priority patent/RU2449007C2/ru
Priority to MXPA06009572A priority patent/MXPA06009572A/es
Priority to CN2005800074288A priority patent/CN1930277B/zh
Priority to ES05715462T priority patent/ES2421440T3/es
Publication of WO2005083093A2 publication Critical patent/WO2005083093A2/de
Publication of WO2005083093A3 publication Critical patent/WO2005083093A3/de
Priority to IL177231A priority patent/IL177231A/en
Priority to US14/823,253 priority patent/US10035989B2/en

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Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung von mehrfach ungesättigten Fettsäuren im Samen transgener Pflanzen, indem Nukleinsäuren in den Organismus eingebracht werden, die für Polypeptide mit ω-3-Desaturase-, Δ-12-Desaturase-, Δ-6-Desaturase-, Δ-6-Elongase-, Δ-5-Desaturase-, Δ-5-Elongase- und/oder Δ-4-Desaturaseaktivität bevorzugt für Polypeptide mit Δ-6-Desaturase-, Δ-6-Elongase- und Δ-5-Desaturaseaktivität codieren. Bei den Nukleinsäuresequenzen handelt es sich um die in SEQ ID NO: 11, SEQ ID NO: 27, SEQ ID NO: 193, SEQ ID NO: 197, SEQ ID NO: 199 und SEQ ID NO: 201 dargestellten Sequenzen. Vorteilhaft können diese Nukleinsäuresequenzen gegebenenfalls zusammen mit weiteren Nukleinsäuresequenzen, die für Polypeptide der Biosynthese des Fettsäure- oder Lipidstoffwechels codieren, in dem Organismus exprimiert werden. Besonders vorteilhaft sind Nukleinsäuresequenzen, die für eine Δ-6-Desaturase-, eine Δ-5-Desaturase-, Δ-4-Desaturase-, Δ-1 2-Desaturase- und/oder Δ-6-Elongaseaktivität codieren. Vorteilhaft stammen diese Desaturasen und Elongasen aus Thalassiosira, Euglena oder Ostreococcus. Weiterhin betrifft die Erfindung ein Verfahren zur Herstellung von Ölen und/oder Triacylglyceriden mit einem erhöhten Gehalt an langkettigen mehrfach ungesättigten Fettsäuren. Die Erfindung betrifft in einer bevorzugten Ausführungsform ausserdem ein Verfahren zur Herstellung von Arachidonsäure, Eicosapentaensäure oder Docosahexaensäure sowie ein Verfahren zur Herstellung von Triglyceriden mit einem erhöhten Gehalt an ungesättigten Fettsäuren, insbesondere Arachidonsäure, Eicosapentaensäure und/oder Docosahexaensäure, in transgenen Pflanzen vorteilhaft im Samen der transgenen Pflanze. Die Erfindung betrifft die Herstellung einer transgenen Pflanze mit erhöhtem Gehalt an mehrfach ungesättigten Fettsäuren, insbesondere Arachidonsäure, Eicosapentaensäure und/oder Docosahexaensäure, aufgrund der Expression der im erfindungsgemässen Verfahren verwendeten Elongasen und Desaturasen. Die Erfindung betrifft weiterhin rekombinante Nukleinsäuremoleküle, die die Nukleinsäuresequenzen, die für die Polypeptide mit Δ-6-Desaturase-, Δ-6-Elongase-, Δ-5-Desaturase- und Δ-5-Elongaseaktivität kodieren, gemeinsam oder einzeln enthalten, sowie transgene Pflanzen, die die vorgenannten rekombinanten Nukleinsäuremoleküle enthalten. Ein weiterer Teil der Erfindung betrifft Öle, Lipide und/oder Fettsäuren hergestellt nach dem erfindungsgemässen Verfahren und deren Verwendung. Ausserdem betrifft die Erfindung ungesättigte Fettsäuren sowie Triglyceride mit einem erhöhten Gehalt an ungesättigten Fettsäuren und deren Verwendung.
PCT/EP2005/001863 2004-02-27 2005-02-23 Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen WO2005083093A2 (de)

Priority Applications (15)

Application Number Priority Date Filing Date Title
RU2006134267/10A RU2449007C2 (ru) 2004-02-27 2005-02-23 Способ получения полиненасыщенных жирных кислот в трансгенных растениях
MXPA06009572A MXPA06009572A (es) 2004-02-27 2005-02-23 Metodo para la produccion de acidos grasos poliinsaturados en plantas transgenicas.
CN2005800074288A CN1930277B (zh) 2004-02-27 2005-02-23 在转基因植物中产生多不饱和脂肪酸的方法
ES05715462T ES2421440T3 (es) 2004-02-27 2005-02-23 Método para preparar ácidos grasos poliinsaturados en plantas transgénicas
CA002559360A CA2559360A1 (en) 2004-02-27 2005-02-23 Method for producing c18, c20 and c22 polyunsaturated fatty acids in transgenic plants
EP19155597.8A EP3543324B1 (de) 2004-02-27 2005-02-23 Verfahren zur herstellung mehrfach ungesättigten fettsäuren in transgenen pflanzen
PL05715462T PL1723220T3 (pl) 2004-02-27 2005-02-23 Sposób wytwarzania wielokrotnie nienasyconych kwasów tłuszczowych w roślinach transgenicznych
AU2005217079A AU2005217079C1 (en) 2004-02-27 2005-02-23 Method for producing polyunsaturated fatty acids in transgenic plants
EP13162907.3A EP2623584B1 (de) 2004-02-27 2005-02-23 Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Pflanzen
EP22209519.2A EP4219670A3 (de) 2004-02-27 2005-02-23 Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen
EP05715462.7A EP1723220B1 (de) 2004-02-27 2005-02-23 Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen
JP2007500134A JP4567047B2 (ja) 2004-02-27 2005-02-23 トランスジェニック植物における多価不飽和脂肪酸の製造方法
US10/590,457 US9458436B2 (en) 2004-02-27 2005-02-23 Method for producing polyunsaturated fatty acids in transgenic plants
IL177231A IL177231A (en) 2004-02-27 2006-08-02 A method of producing unsaturated fatty acid poly in transgenic plants
US14/823,253 US10035989B2 (en) 2004-02-27 2015-08-11 Method for producing polyunsaturated fatty acids in transgenic plants

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
DE102004009457 2004-02-27
DE102004009457.8 2004-02-27
DE102004012370.5 2004-03-13
DE102004012370 2004-03-13
DE102004017518A DE102004017518A1 (de) 2004-04-08 2004-04-08 Verfahren zur Herstellung von mehrfach ungesättigten Fettsäuren
DE102004017518.7 2004-04-08
DE102004024014 2004-05-14
DE102004024014.0 2004-05-14
EPPCT/EP/04/07957 2004-07-16
PCT/EP2004/007957 WO2005012316A2 (de) 2003-08-01 2004-07-16 Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen
DE200410062543 DE102004062543A1 (de) 2004-12-24 2004-12-24 Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Pflanzen
DE102004062543.3 2004-12-24

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/590,457 A-371-Of-International US9458436B2 (en) 2004-02-27 2005-02-23 Method for producing polyunsaturated fatty acids in transgenic plants
US14/823,253 Continuation US10035989B2 (en) 2004-02-27 2015-08-11 Method for producing polyunsaturated fatty acids in transgenic plants

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WO2005083093A2 WO2005083093A2 (de) 2005-09-09
WO2005083093A3 true WO2005083093A3 (de) 2006-02-16

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EP (2) EP1723220B1 (de)
AU (1) AU2005217079C1 (de)
CA (2) CA3045858C (de)
PL (1) PL1723220T3 (de)
RU (1) RU2449007C2 (de)
WO (1) WO2005083093A2 (de)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8853432B2 (en) 2004-04-22 2014-10-07 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
US9453183B2 (en) 2004-04-22 2016-09-27 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
US9550718B2 (en) 2012-06-15 2017-01-24 Commonwealth Scientific And Industrial Research Organisation Lipid comprising polyunsaturated fatty acids

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2166090B1 (de) 2003-08-01 2015-07-01 BASF Plant Science GmbH Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen
US11952581B2 (en) 2003-08-01 2024-04-09 Basf Plant Science Gmbh Process for the production of polyunsaturated fatty acids in transgenic organisms
EP3543324B1 (de) 2004-02-27 2022-11-30 BASF Plant Science GmbH Verfahren zur herstellung mehrfach ungesättigten fettsäuren in transgenen pflanzen
CA2558726C (en) 2004-02-27 2017-11-07 Basf Plant Science Gmbh Method for producing unsaturated .omega.-3-fatty acids in transgenic organisms
AU2005235627B2 (en) * 2004-04-22 2011-07-07 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
AU2011232757B2 (en) * 2004-04-22 2013-10-03 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
US7550651B2 (en) 2004-06-25 2009-06-23 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
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