MX338036B - Expresion proteinica a partir de acidos nucleicos multiples. - Google Patents

Expresion proteinica a partir de acidos nucleicos multiples.

Info

Publication number
MX338036B
MX338036B MX2013003820A MX2013003820A MX338036B MX 338036 B MX338036 B MX 338036B MX 2013003820 A MX2013003820 A MX 2013003820A MX 2013003820 A MX2013003820 A MX 2013003820A MX 338036 B MX338036 B MX 338036B
Authority
MX
Mexico
Prior art keywords
cho cell
selection agent
nucleic acid
eukaryotic selection
transfecting
Prior art date
Application number
MX2013003820A
Other languages
English (en)
Inventor
Erhard Kopetzki
Anne Stern
Hendrik Knoetgen
Ulrich Goepfert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39204855&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX338036(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX338036B publication Critical patent/MX338036B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2812—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • C07K16/2854—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72 against selectins, e.g. CD62
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL

Abstract

La presente invención se refiere a un método para la producción recombinante de una inmunoglobulina heteróloga secretada en una célula CHO que comprende las siguientes etapas: i) proporcionar una célula CHO la cual está adaptada para crecer en cultivo en suspensión, adaptada para crecer en medio libre de suero, libre de micoplasmas y libre de virus, ii) proporcionar un vector que comprende un origen de replicación procariótico, un primer ácido nucleico que confiere resistencia contra un agente de selección procariótico, un segundo ácido nucleico que codifica para la cadena pesada de la inmunoglobulina heteróloga, un tercer ácido nucleico que codifica para la cadena ligera de la inmunoglobulina heteróloga, un cuarto ácido nucleico que confiere resistencia contra un agente de selección eucariótico, iii) transfectar la célula CHO, en donde la transfección comprende a) transfectar la célula CHO con el vector que comprende un cuarto ácido nucleico que confiere resistencia contra un primer agente de selección eucariótico, b) seleccionar una célula CHO por crecimiento en medio de cultivo que contiene el primer agente de selección eucariótico, c) transfectar la célula CHO seleccionada con el vector que comprende un cuarto ácido nucleico que confiere resistencia contra un segundo agente de selección eucariótico diferente del primer agente de selección eucariótico, d) seleccionar una célula CHO mediante crecimiento selectivo en medio de cultivo que contiene el primero y segundo agentes de selección eucarióticos, iv) cultivar la célula CHO transfectada en un medio en presencia del primero y segundo agentes de selección eucarióticos bajo condiciones adecuadas para la expresión del segundo y tercer ácidos nucleicos, y v) recuperar la inmunoglobulina heteróloga secretada del medio de cultivo.
MX2013003820A 2007-10-12 2008-10-09 Expresion proteinica a partir de acidos nucleicos multiples. MX338036B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07019999 2007-10-12
PCT/EP2008/008523 WO2009046978A1 (en) 2007-10-12 2008-10-09 Protein expression from multiple nucleic acids

Publications (1)

Publication Number Publication Date
MX338036B true MX338036B (es) 2016-03-31

Family

ID=39204855

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2010003622A MX2010003622A (es) 2007-10-12 2008-10-09 Expresion proteinica a partir de acidos nucleicos multiples.
MX2013003820A MX338036B (es) 2007-10-12 2008-10-09 Expresion proteinica a partir de acidos nucleicos multiples.
MX2010003620A MX2010003620A (es) 2007-10-12 2008-10-13 Linea de celulas cho-k1.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2010003622A MX2010003622A (es) 2007-10-12 2008-10-09 Expresion proteinica a partir de acidos nucleicos multiples.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2010003620A MX2010003620A (es) 2007-10-12 2008-10-13 Linea de celulas cho-k1.

Country Status (20)

Country Link
US (5) US8771988B2 (es)
EP (4) EP2592147A1 (es)
JP (2) JP5642549B2 (es)
KR (2) KR101280704B1 (es)
CN (2) CN101815786A (es)
AU (2) AU2008309934B2 (es)
BR (2) BRPI0818165B8 (es)
CA (2) CA2701646C (es)
DK (1) DK2592148T3 (es)
ES (1) ES2695999T3 (es)
HR (1) HRP20181833T1 (es)
HU (1) HUE039950T2 (es)
IL (2) IL203900A (es)
LT (1) LT2592148T (es)
MX (3) MX2010003622A (es)
PL (1) PL2592148T3 (es)
PT (1) PT2592148T (es)
SI (1) SI2592148T1 (es)
TR (1) TR201816045T4 (es)
WO (2) WO2009046978A1 (es)

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Also Published As

Publication number Publication date
BRPI0818165B8 (pt) 2021-05-25
IL203900A0 (en) 2011-07-31
JP2011500009A (ja) 2011-01-06
CA2701677A1 (en) 2009-04-16
PL2592148T3 (pl) 2019-01-31
KR101280704B1 (ko) 2013-07-01
US20170166923A1 (en) 2017-06-15
IL203902A0 (en) 2011-07-31
KR20100059969A (ko) 2010-06-04
HUE039950T2 (hu) 2019-02-28
BRPI0818536A2 (pt) 2014-10-14
WO2009046978A1 (en) 2009-04-16
MX2010003622A (es) 2010-04-14
AU2008309881A1 (en) 2009-04-16
CN101815786A (zh) 2010-08-25
AU2008309934A1 (en) 2009-04-16
US20100249379A1 (en) 2010-09-30
JP2010540583A (ja) 2010-12-24
EP2207881A1 (en) 2010-07-21
SI2592148T1 (sl) 2018-12-31
PT2592148T (pt) 2018-11-12
MX2010003620A (es) 2010-04-14
IL203900A (en) 2014-08-31
HRP20181833T1 (hr) 2019-01-11
US20190233842A1 (en) 2019-08-01
CA2701646C (en) 2019-02-26
US9428766B2 (en) 2016-08-30
EP2207882A1 (en) 2010-07-21
AU2008309934B2 (en) 2014-03-06
EP2592148B1 (en) 2018-09-12
JP5642549B2 (ja) 2014-12-17
EP2592148A1 (en) 2013-05-15
CA2701646A1 (en) 2009-04-16
ES2695999T3 (es) 2019-01-11
KR20100059967A (ko) 2010-06-04
LT2592148T (lt) 2018-12-10
EP2592147A1 (en) 2013-05-15
US20100221781A1 (en) 2010-09-02
DK2592148T3 (en) 2018-12-10
BRPI0818165A2 (pt) 2014-10-14
TR201816045T4 (tr) 2018-11-21
CN101821394A (zh) 2010-09-01
BRPI0818165B1 (pt) 2020-10-27
US8771988B2 (en) 2014-07-08
US20140335609A1 (en) 2014-11-13
WO2009047007A1 (en) 2009-04-16

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