DE602004023408D1 - Agments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments - Google Patents

Agments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments

Info

Publication number
DE602004023408D1
DE602004023408D1 DE602004023408T DE602004023408T DE602004023408D1 DE 602004023408 D1 DE602004023408 D1 DE 602004023408D1 DE 602004023408 T DE602004023408 T DE 602004023408T DE 602004023408 T DE602004023408 T DE 602004023408T DE 602004023408 D1 DE602004023408 D1 DE 602004023408D1
Authority
DE
Germany
Prior art keywords
antibody fragment
signal sequence
agments
mass production
coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004023408T
Other languages
English (en)
Inventor
Sung Youb Jung
Jin Sun Kim
Se Chang Kwon
Gwan Sun Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hanmi Science Co Ltd
Original Assignee
Hanmi Pharmaceutical Co Ltd
Hanmi Pharmaceutical Industries Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hanmi Pharmaceutical Co Ltd, Hanmi Pharmaceutical Industries Co Ltd filed Critical Hanmi Pharmaceutical Co Ltd
Publication of DE602004023408D1 publication Critical patent/DE602004023408D1/de
Anticipated expiration legal-status Critical
Active legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE602004023408T 2003-10-16 2004-10-14 Agments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments Active DE602004023408D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020030072216A KR100570422B1 (ko) 2003-10-16 2003-10-16 대장균 분비서열을 이용하여 항체 단편을 분비·생산하는 발현벡터 및 이를 이용하여 항체 단편을 대량 생산하는 방법
PCT/KR2004/002625 WO2005038031A1 (en) 2003-10-16 2004-10-14 Expression vector for secreting antibody fragment using e. coli signal sequence and method for mass-producing antibody fragment

Publications (1)

Publication Number Publication Date
DE602004023408D1 true DE602004023408D1 (de) 2009-11-12

Family

ID=36569730

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004023408T Active DE602004023408D1 (de) 2003-10-16 2004-10-14 Agments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments

Country Status (8)

Country Link
US (1) US7718399B2 (de)
EP (1) EP1678308B1 (de)
JP (1) JP4448138B2 (de)
KR (1) KR100570422B1 (de)
AT (1) ATE444370T1 (de)
DE (1) DE602004023408D1 (de)
ES (1) ES2331573T3 (de)
WO (1) WO2005038031A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8952215B2 (en) 2005-10-18 2015-02-10 Nitto Boseki Co., Ltd. Antibody-producing transgenic silkworms and methods for producing the same
DE102006004871A1 (de) * 2006-02-02 2007-08-09 Wacker Chemie Ag Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
KR20080110833A (ko) * 2006-03-21 2008-12-19 와이어쓰 아밀로이드 형성 질환의 예방 및 치료 방법
ATE501266T1 (de) 2006-09-22 2011-03-15 Wacker Chemie Ag Verfahren zur fermentativen herstellung von antikörpern
CA2780143A1 (en) * 2009-11-05 2011-05-12 Genentech, Inc. Methods and composition for secretion of heterologous polypeptides
EP2817625A2 (de) 2012-02-23 2014-12-31 Stage Cell Therapeutics GmbH Chromatografische isolierung von zellen und anderen komplexen biologischen materialien
PL3116999T3 (pl) * 2014-03-14 2021-12-27 F.Hoffmann-La Roche Ag Sposoby i kompozycje do wydzielania polipeptydów heterologicznych
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
TW201842335A (zh) 2017-04-27 2018-12-01 德商朱諾醫療公司 寡聚粒子試劑及其使用方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7018809B1 (en) * 1991-09-19 2006-03-28 Genentech, Inc. Expression of functional antibody fragments
WO1994028025A1 (en) * 1993-05-28 1994-12-08 The Scripps Research Institute Methods and compositions for inhibiting cd14 mediated cell activation
KR100316347B1 (ko) * 1998-09-15 2002-08-27 한미약품(주) 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법
GB0013810D0 (en) * 2000-06-06 2000-07-26 Celltech Chiroscience Ltd Biological products
NZ530852A (en) * 2001-08-27 2006-11-30 Genentech Inc Methods and compositions for recombinantly producing functional antibodies or antibody fragments in prokaryotic and eukaryotic host cells

Also Published As

Publication number Publication date
US7718399B2 (en) 2010-05-18
JP2007508032A (ja) 2007-04-05
US20090104660A1 (en) 2009-04-23
KR20050036517A (ko) 2005-04-20
EP1678308A4 (de) 2007-07-11
KR100570422B1 (ko) 2006-04-11
WO2005038031A1 (en) 2005-04-28
EP1678308B1 (de) 2009-09-30
ATE444370T1 (de) 2009-10-15
JP4448138B2 (ja) 2010-04-07
EP1678308A1 (de) 2006-07-12
ES2331573T3 (es) 2010-01-08

Similar Documents

Publication Publication Date Title
DK0786009T3 (da) Fremgangsmåde til bakteriel fremstilling af heterologe polypeptider med disulphidbindinger
ATE481483T1 (de) Verfahren zur herstellung eines rekombinanten polyklonalen proteins
HRP20240408T1 (hr) Inzulin-fc fuzijski proteini ultradugog djelovanja i metode uporabe
ATE359358T1 (de) Verfahren und medien zur kontrolle der sialysierung von proteinen, die von säugetierzellen produziert werden
GB2438760A (en) Methods for expression and purification of recombinant human growth hormone
ATE368730T1 (de) Verfahren zur herstelllung rekombinanter proteine in eukaryontischen zellen
DE602006018587D1 (de) Verfahren zur verbesserten isolierung rekombinant produzierter proteine
ATE512230T1 (de) Proteinherstellungsverfahren unter nutzung von yebf
DE502004008542D1 (de) Verfahren zur reklonierung von produktionszellen
CY1110494T1 (el) Μεταστροφες μεταβολικου ρυθμου σε ζυμωσεις που εκφραζουν ανασυνδυασμενες πρωτεϊνες
ATE469917T2 (de) Verfahren zur gewinnung von antikörpern
DE602004023408D1 (de) Agments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments
ATE486944T1 (de) Translationselongationsfaktor-promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davon
HUP0303843A2 (hu) CLyA-hemolizin alkalmazása proteinek exkretáltatására
TW200638944A (en) Recombinant enterovirus 71 neutralizing antibodies and use thereof
ATE478140T1 (de) Verfahren zur herstellung sezernierter polypeptide
EA201000945A1 (ru) Конструирование системы секреции на основе sm-белков
DE60021404D1 (de) System zur herstellung von proteinen
DE60214127D1 (de) Hybridomzelllinie g250 und deren verwendung zur herstellung monoklonaler antikörper
ATE439432T1 (de) Verfahren zur herstellung von säugetier-trypsinen
EA201070372A1 (ru) СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОГО с-ПЕПТИДА ПРОИНСУЛИНА ЧЕЛОВЕКА
DE60040789D1 (de) Verfahren zur aufreinigung heterologer proteine
DE69930118D1 (de) Verfahren zur herstellung von proteinen in transformierten hefezellen
CN102443600B (zh) 一种重组可溶性hSHH-N蛋白的制备方法
WO2003012098A1 (fr) Levure modifiee presentant un potentiel secretoire eleve de proteine etrangere et procede permettant de produire une proteine etrangere a l'aide de la levure

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: HANMI HOLDINGS CO., LTD., SEOUL, KR

R081 Change of applicant/patentee

Ref document number: 1678308

Country of ref document: EP

Owner name: HANMI SCIENCE CO., LTD., KR

Free format text: FORMER OWNER: HANMI HOLDINGS CO., LTD., SEOUL, KR

Effective date: 20120906

R082 Change of representative

Ref document number: 1678308

Country of ref document: EP

Representative=s name: GRUENECKER, KINKELDEY, STOCKMAIR & SCHWANHAEUS, DE

Effective date: 20120906