HUP0303843A2 - CLyA-hemolizin alkalmazása proteinek exkretáltatására - Google Patents

CLyA-hemolizin alkalmazása proteinek exkretáltatására

Info

Publication number
HUP0303843A2
HUP0303843A2 HU0303843A HUP0303843A HUP0303843A2 HU P0303843 A2 HUP0303843 A2 HU P0303843A2 HU 0303843 A HU0303843 A HU 0303843A HU P0303843 A HUP0303843 A HU P0303843A HU P0303843 A2 HUP0303843 A2 HU P0303843A2
Authority
HU
Hungary
Prior art keywords
protein
export
proteins
clya
hemolysin
Prior art date
Application number
HU0303843A
Other languages
English (en)
Inventor
James E. Galen
Original Assignee
University Of Maryland, Baltimore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Maryland, Baltimore filed Critical University Of Maryland, Baltimore
Publication of HUP0303843A2 publication Critical patent/HUP0303843A2/hu
Publication of HUP0303843A3 publication Critical patent/HUP0303843A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • External Artificial Organs (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A találmány protein-exportrendszer alkalmazására vonatkozik. Atalálmány tárgyát hatásos eljárások és készítmények képezikrekombináns proteinek előállítására. A találmány szerinti expressziósrendszerben a kívánt proteint úgy expresszáltatják, hogy bakteriálissejtekbe a kívánt proteint kódoló szekvenciával fuzionáltatottexportproteint kódoló szekvenciát tartalmazó expressziós kazettáttartalmazó expressziós vektort jutattnak, az expreszziós kazettátexpresszáltatják, és ily módon az exportproteint és a kívánt proteinttartalmazó fúziós proteint kapnak, amely a tenyésztőközegbeexportálódik. Ó
HU0303843A 2000-11-22 2001-11-23 Use of clya hemolysin for excretion of proteins HUP0303843A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25251600P 2000-11-22 2000-11-22
PCT/US2001/044185 WO2002083890A2 (en) 2000-11-22 2001-11-23 Use of clya hemolysin for excretion of fusion proteins

Publications (2)

Publication Number Publication Date
HUP0303843A2 true HUP0303843A2 (hu) 2004-03-01
HUP0303843A3 HUP0303843A3 (en) 2010-01-28

Family

ID=22956340

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0303843A HUP0303843A3 (en) 2000-11-22 2001-11-23 Use of clya hemolysin for excretion of proteins

Country Status (16)

Country Link
US (2) US7056700B2 (hu)
EP (1) EP1412502B1 (hu)
JP (1) JP3976685B2 (hu)
AT (1) ATE474926T1 (hu)
AU (1) AU2001297770B2 (hu)
CA (1) CA2430322A1 (hu)
CZ (1) CZ20031378A3 (hu)
DE (1) DE60142646D1 (hu)
ES (1) ES2349114T3 (hu)
HU (1) HUP0303843A3 (hu)
MX (1) MXPA03004562A (hu)
NO (1) NO20032286L (hu)
NZ (1) NZ525735A (hu)
PL (1) PL366122A1 (hu)
WO (1) WO2002083890A2 (hu)
ZA (1) ZA200303851B (hu)

Families Citing this family (22)

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Publication number Priority date Publication date Assignee Title
DE10208653A1 (de) * 2002-02-28 2003-09-18 Medinnova Ges Med Innovationen Mikroorganismus als Träger von Nukleotidsequenzen kodierend für Zellantigene zur Behandlung von Tumoren
US20080124355A1 (en) * 2006-09-22 2008-05-29 David Gordon Bermudes Live bacterial vaccines for viral infection prophylaxis or treatment
WO2008147816A2 (en) * 2007-05-22 2008-12-04 Cornell Research Foundation, Inc. Compositions and methods for the display of proteins on the surface of bacteria and their derived vesicles and uses thereof
CN105950608B (zh) * 2007-05-22 2019-12-17 康乃尔研究基金会有限公司 蛋白质在细菌及其衍生小泡表面展示的组合物和方法及其用途
CA2726293C (en) * 2008-06-03 2017-02-28 University Of Maryland, Baltimore Non-hemolytic clya for excretion of proteins
US20120020996A1 (en) * 2008-08-06 2012-01-26 Jonathan Lewis Telfer Vaccines against clostridium difficile and methods of use
US8211656B2 (en) 2008-08-13 2012-07-03 The Invention Science Fund I, Llc Biological targeting compositions and methods of using the same
US20100040546A1 (en) * 2008-08-13 2010-02-18 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Biological targeting compositions and methods of using the same
US20120034155A1 (en) * 2010-08-03 2012-02-09 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Artificial cells
US20100042072A1 (en) * 2008-08-13 2010-02-18 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Biological targeting compositions and methods of using the same
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
US10046040B2 (en) * 2009-11-16 2018-08-14 University Of Maryland, Baltimore Multivalent live vector vaccine against Clostridium difficile-associated disease
US8771669B1 (en) 2010-02-09 2014-07-08 David Gordon Bermudes Immunization and/or treatment of parasites and infectious agents by live bacteria
EP2833911A4 (en) 2012-04-06 2016-07-13 Univ Cornell SUB-VACCINE VACCINATING PLATFORM FOR ROBUST HUMORAL AND CELLULAR IMMUNE RESPONSES
MX2016012260A (es) * 2014-03-28 2017-01-06 Novozymes As Resolubilizacion de cristales de proteina a bajo ph.
AU2015252119A1 (en) 2014-11-07 2016-05-26 Takeda Vaccines, Inc. Hand, foot, and mouth vaccines and methods of manufacture and use thereof
AR102547A1 (es) 2014-11-07 2017-03-08 Takeda Vaccines Inc Vacunas contra la enfermedad de manos, pies y boca y métodos de fabricación y su uso
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
EP3703741A1 (en) 2017-11-03 2020-09-09 Takeda Vaccines, Inc. Zika vaccines and immunogenic compositions, and methods of using the same
CN110747155A (zh) * 2019-11-12 2020-02-04 湖南大学 减毒重组工程菌及其制备方法、应用和肿瘤靶向药物
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

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US4235877A (en) * 1979-06-27 1980-11-25 Merck & Co., Inc. Liposome particle containing viral or bacterial antigenic subunit
US4372945A (en) * 1979-11-13 1983-02-08 Likhite Vilas V Antigen compounds
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IL61904A (en) * 1981-01-13 1985-07-31 Yeda Res & Dev Synthetic vaccine against influenza virus infections comprising a synthetic peptide and process for producing same
US4665027A (en) * 1983-11-03 1987-05-12 Bio-Process Innovation, Inc. Immobilized cell reactor-separator with simultaneous product separation and methods for design and use thereof
US5387744A (en) * 1987-06-04 1995-02-07 Washington University Avirulent microbes and uses therefor: Salmonella typhi
KR890005273A (ko) * 1987-09-09 1989-05-13 이정오 2중 실관 반응기에 의한 구연산의 연속 생산방법
DE4239612A1 (de) * 1992-11-25 1994-05-26 Cultor Oy Bioreaktor mit immobilisierten, Milchsäure-produzierenden Bakterien und dessen Verwendung in Fermentationsverfahren
US5525504A (en) * 1993-04-30 1996-06-11 Merck Patent Gesellschaft Mit Beschrankter Haftung Cytolysin gene and gene product
US5731151A (en) * 1993-05-24 1998-03-24 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Regulator of contact-mediated hemolysin
US5512480A (en) * 1994-03-11 1996-04-30 Baxter International Inc. Flow-through bioreactor with grooves for cell retention
US5585266A (en) * 1995-10-05 1996-12-17 Plitt; Cheryl A. Immobilized cell bioreactor
US6413768B1 (en) * 1998-12-02 2002-07-02 University Of Maryland Expression plasmids
US6436703B1 (en) 2000-03-31 2002-08-20 Hyseq, Inc. Nucleic acids and polypeptides

Also Published As

Publication number Publication date
ZA200303851B (en) 2004-05-11
NO20032286L (no) 2003-07-07
ATE474926T1 (de) 2010-08-15
ES2349114T3 (es) 2010-12-28
NO20032286D0 (no) 2003-05-21
DE60142646D1 (en) 2010-09-02
US20060147461A1 (en) 2006-07-06
US7459161B2 (en) 2008-12-02
EP1412502A2 (en) 2004-04-28
US7056700B2 (en) 2006-06-06
MXPA03004562A (es) 2003-09-10
EP1412502B1 (en) 2010-07-21
HUP0303843A3 (en) 2010-01-28
JP3976685B2 (ja) 2007-09-19
AU2001297770B2 (en) 2007-04-05
WO2002083890A2 (en) 2002-10-24
CA2430322A1 (en) 2002-10-24
PL366122A1 (en) 2005-01-24
US20020146430A1 (en) 2002-10-10
CZ20031378A3 (cs) 2003-09-17
NZ525735A (en) 2005-07-29
JP2004532634A (ja) 2004-10-28
WO2002083890A3 (en) 2004-02-26

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Legal Events

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