CY1110494T1 - Μεταστροφες μεταβολικου ρυθμου σε ζυμωσεις που εκφραζουν ανασυνδυασμενες πρωτεϊνες - Google Patents

Μεταστροφες μεταβολικου ρυθμου σε ζυμωσεις που εκφραζουν ανασυνδυασμενες πρωτεϊνες

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Publication number
CY1110494T1
CY1110494T1 CY20091100652T CY091100652T CY1110494T1 CY 1110494 T1 CY1110494 T1 CY 1110494T1 CY 20091100652 T CY20091100652 T CY 20091100652T CY 091100652 T CY091100652 T CY 091100652T CY 1110494 T1 CY1110494 T1 CY 1110494T1
Authority
CY
Cyprus
Prior art keywords
expression
host cells
recombinant host
knowledges
transformation
Prior art date
Application number
CY20091100652T
Other languages
English (en)
Inventor
Dana Andersen
John Joly
Bradley R Snedecor
Original Assignee
Genentech, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22928808&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1110494(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genentech, Inc. filed Critical Genentech, Inc.
Publication of CY1110494T1 publication Critical patent/CY1110494T1/el

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fertilizers (AREA)

Abstract

Η εφεύρεση παρέχει μέθοδο αύξησης της απόδοσης προϊόντος πολυπεπτιδίου ενδιαφέροντος που παράγεται από ανασυνδυασμένα κύτταρα-ξενιστές, όπου η έκφραση του πολυπεπτιδίου από τα ανασυνδυασμένα κύτταρα-ξενιστές ρυθμίζεται από επαγώγιμο σύστημα. Ειδικότερα, η μέθοδος ενέχει καλλιέργεια των ανασυνδυασμένων κυττάρων-ξενιστών υπό συνθήκες υψηλού μεταβολικού και αυξητικού ρυθμού και μετά μείωση του μεταβολικού ρυθμού των ανασυνδυασμένων κυττάρων-ξενιστών κατά τον χρόνο επαγωγής έκφρασης πολυπεπτιδίου. Συγκεκριμένα, η εφεύρεση παρέχει μέθοδο αύξησης της απόδοσης προϊόντος αντισώματος, αυξητικού παράγοντα, ή πρωτεάσης που παράγεται από ανασυνδυασμένο κύτταρο-ξενιστή Ε. coli που ρυθμίζεται από επαγώγιμο σύστημα.
CY20091100652T 2000-11-03 2009-06-23 Μεταστροφες μεταβολικου ρυθμου σε ζυμωσεις που εκφραζουν ανασυνδυασμενες πρωτεϊνες CY1110494T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24596200P 2000-11-03 2000-11-03
EP01987201A EP1332222B1 (en) 2000-11-03 2001-11-01 Metabolic rate shifts in fermentations expressing recombinant proteins

Publications (1)

Publication Number Publication Date
CY1110494T1 true CY1110494T1 (el) 2015-04-29

Family

ID=22928808

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20091100652T CY1110494T1 (el) 2000-11-03 2009-06-23 Μεταστροφες μεταβολικου ρυθμου σε ζυμωσεις που εκφραζουν ανασυνδυασμενες πρωτεϊνες

Country Status (14)

Country Link
US (1) US6716602B2 (el)
EP (1) EP1332222B1 (el)
JP (1) JP4878724B2 (el)
AT (1) ATE426674T1 (el)
AU (1) AU3944002A (el)
CA (1) CA2426100A1 (el)
CY (1) CY1110494T1 (el)
DE (1) DE60138127D1 (el)
DK (1) DK1332222T3 (el)
ES (1) ES2324645T3 (el)
HK (1) HK1054765A1 (el)
IL (2) IL155482A0 (el)
PT (1) PT1332222E (el)
WO (1) WO2002040697A2 (el)

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* Cited by examiner, † Cited by third party
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US7294481B1 (en) 1999-01-05 2007-11-13 Immunex Corporation Method for producing recombinant proteins
AU2003251381B2 (en) * 2002-05-30 2008-04-03 Natureworks Llc Fermentation process using specific oxygen uptake rates as a process control
GB0321100D0 (en) * 2003-09-09 2003-10-08 Celltech R&D Ltd Biological products
BRPI0913475B8 (pt) 2008-09-15 2019-05-07 Genentech Inc vetor
BR112012009828B8 (pt) 2009-10-26 2022-10-25 Hoffmann La Roche Método para a produção de uma imunoglobulina glicosilada e seu uso
GB2482536B (en) * 2010-08-05 2013-07-24 Hera Pharmaceuticals Inc Expression of antibody or a fragment thereof in lactobacillus
EP3502267A1 (en) 2012-09-19 2019-06-26 F. Hoffmann-La Roche AG Methods and compositions for preventing norleucine misincorporation into proteins
DK3030671T3 (en) 2013-08-05 2019-01-21 Greenlight Biosciences Inc MODIFIED PROTEINS WITH A PROTEASE CLEANING PLACE
EP3147369A4 (en) * 2014-05-21 2018-02-07 Ajinomoto Co., Inc. Fibroin-like protein production method
CN104164465B (zh) * 2014-07-14 2017-07-04 北京泰德制药股份有限公司 一种表达重组蛋白的发酵工艺
US10066002B2 (en) * 2014-11-05 2018-09-04 Genentech, Inc. Methods of producing two chain proteins in bacteria
AU2015342961B2 (en) * 2014-11-05 2021-08-12 Genentech, Inc. Methods of producing two chain proteins in bacteria
CN107949632B (zh) 2015-05-29 2021-06-01 嘉吉公司 用于使用高pH生产甜菊醇糖苷的发酵方法和由此获得的组合物
BR112017025709B1 (pt) 2015-05-29 2024-03-05 Cargill, Incorporated Método para produzir glicosídeos de esteviol
CN107613786A (zh) 2015-05-29 2018-01-19 嘉吉公司 产生糖苷的热处理
CN107920548A (zh) 2015-08-06 2018-04-17 嘉吉公司 用于产生甜菊醇糖苷的发酵方法
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
US11028410B2 (en) 2016-01-27 2021-06-08 Just-Evotec Biologics, Inc. Hybrid promoter and uses thereof
US11098310B2 (en) 2016-01-27 2021-08-24 Just-Evotec Biologics, Inc. Expression from transposon-based vectors and uses
US11261462B2 (en) 2016-01-27 2022-03-01 Just-Evotec Biologics, Inc. Inducible expression from transposon-based vectors and uses
KR102606210B1 (ko) 2017-04-27 2023-11-24 주노 테라퓨틱스 게엠베하 올리고머 입자 시약 및 이의 사용 방법

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US4565785A (en) 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
US4673641A (en) 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
US4710473A (en) 1983-08-10 1987-12-01 Amgen, Inc. DNA plasmids
US4738921A (en) 1984-09-27 1988-04-19 Eli Lilly And Company Derivative of the tryptophan operon for expression of fused gene products
US4795706A (en) 1985-01-31 1989-01-03 Eli Lilly And Company Novel expression control sequences
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
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Also Published As

Publication number Publication date
AU3944002A (en) 2002-05-27
PT1332222E (pt) 2009-06-30
DE60138127D1 (de) 2009-05-07
ATE426674T1 (de) 2009-04-15
CA2426100A1 (en) 2002-05-23
WO2002040697A2 (en) 2002-05-23
US6716602B2 (en) 2004-04-06
EP1332222A2 (en) 2003-08-06
JP4878724B2 (ja) 2012-02-15
WO2002040697A3 (en) 2003-03-13
DK1332222T3 (da) 2009-07-06
JP2004517614A (ja) 2004-06-17
US20020164700A1 (en) 2002-11-07
HK1054765A1 (en) 2003-12-12
ES2324645T3 (es) 2009-08-12
IL155482A0 (en) 2003-11-23
IL155482A (en) 2010-12-30
EP1332222B1 (en) 2009-03-25

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