MX2015007131A - Ensayos rapidos de bajo volumen de muestra de colesterol y trigliceridos. - Google Patents

Ensayos rapidos de bajo volumen de muestra de colesterol y trigliceridos.

Info

Publication number
MX2015007131A
MX2015007131A MX2015007131A MX2015007131A MX2015007131A MX 2015007131 A MX2015007131 A MX 2015007131A MX 2015007131 A MX2015007131 A MX 2015007131A MX 2015007131 A MX2015007131 A MX 2015007131A MX 2015007131 A MX2015007131 A MX 2015007131A
Authority
MX
Mexico
Prior art keywords
cholesterol
assays
density lipoprotein
sub
fractions
Prior art date
Application number
MX2015007131A
Other languages
English (en)
Other versions
MX364956B (es
Inventor
Paul Patel
Douglas Matje
Ian Gibbons
Elizabeth A Holmes
Original Assignee
Theranos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Theranos Inc filed Critical Theranos Inc
Publication of MX2015007131A publication Critical patent/MX2015007131A/es
Publication of MX364956B publication Critical patent/MX364956B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/90—Developer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/90—Developer
    • C12Q2326/96—4-Amino-antipyrine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/115—Characterised by chemical treatment oxidising agents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/908—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/918—Carboxylic ester hydrolases (3.1.1)
    • G01N2333/92—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)

Abstract

Se proporcionan reactivos, ensayos, métodos, equipos, dispositivos y sistemas para la medición rápida de colesterol y sub-fracciones de colesterol de una muestra de sangre. Se pueden medir el colesterol total, lipoproteína de baja densidad-colesterol, y lipoproteína de alta densidad-colesterol en un ensayo individual usando mediciones cinéticas, bajo condiciones en las cuales se convierten a sub-especies de colesterol a un producto detectable a distintas velocidades. El producto detectable se mide en diferentes momentos después del inicio del ensayo. Se puede usar una lipasa, una colesterol-esterasa, una colesterol-oxidasa y una peroxidasa conjuntamente para producir el producto coloreado en cantidades directamente proporcionales a la cantidad de colesterol convertido. Los métodos para calcular los niveles de lipoproteína de muy baja densidad-colesterol al incluir además mediciones de triglicéridos se describen. Los ensayos se pueden realizar en una mezcla de reacción individual, permitiendo determinaciones más exactas y precisas de colesterol, incluyendo relaciones de sub-fracciones de colesterol a colesterol total, a menos costo, creo que se esperaría realizar varios ensayos diferentes en diferentes mezclas de reacción.
MX2015007131A 2012-12-10 2013-12-10 Ensayos rapidos de bajo volumen de muestra de colesterol y trigliceridos. MX364956B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261735424P 2012-12-10 2012-12-10
PCT/US2013/074211 WO2014093399A1 (en) 2012-12-10 2013-12-10 Rapid, low-sample-volume cholesterol and triglyceride assays

Publications (2)

Publication Number Publication Date
MX2015007131A true MX2015007131A (es) 2015-10-14
MX364956B MX364956B (es) 2019-05-15

Family

ID=50931352

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015007131A MX364956B (es) 2012-12-10 2013-12-10 Ensayos rapidos de bajo volumen de muestra de colesterol y trigliceridos.

Country Status (13)

Country Link
US (4) US9051599B2 (es)
EP (2) EP2929046B1 (es)
JP (4) JP2016505250A (es)
KR (1) KR20150094710A (es)
CN (2) CN104995310B (es)
AU (1) AU2013359422A1 (es)
CA (1) CA2891944A1 (es)
ES (1) ES2729794T3 (es)
HK (1) HK1216763A1 (es)
IL (1) IL239229A (es)
MX (1) MX364956B (es)
SG (1) SG11201504455PA (es)
WO (1) WO2014093399A1 (es)

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CN109799356A (zh) * 2018-12-28 2019-05-24 上海高踪医疗器械科技有限公司 一种测定高密度脂蛋白胆固醇的试剂盒及方法
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Also Published As

Publication number Publication date
JP2021006060A (ja) 2021-01-21
IL239229A (en) 2017-02-28
EP2929046A4 (en) 2016-06-29
CN104995310B (zh) 2020-07-28
KR20150094710A (ko) 2015-08-19
JP2016505250A (ja) 2016-02-25
US20160024554A1 (en) 2016-01-28
ES2729794T3 (es) 2019-11-06
JP2023015207A (ja) 2023-01-31
CN104995310A (zh) 2015-10-21
US10392650B2 (en) 2019-08-27
JP2019054811A (ja) 2019-04-11
US11268130B2 (en) 2022-03-08
EP2929046B1 (en) 2019-03-20
MX364956B (es) 2019-05-15
US20200063185A1 (en) 2020-02-27
CA2891944A1 (en) 2014-06-19
US20140170688A1 (en) 2014-06-19
WO2014093399A1 (en) 2014-06-19
US9546394B2 (en) 2017-01-17
IL239229A0 (en) 2015-07-30
AU2013359422A1 (en) 2015-06-04
EP2929046A1 (en) 2015-10-14
US20170175168A1 (en) 2017-06-22
US9051599B2 (en) 2015-06-09
HK1216763A1 (zh) 2016-12-02
CN111748605A (zh) 2020-10-09
EP3561068A1 (en) 2019-10-30
SG11201504455PA (en) 2015-07-30

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