BR112013033082A2 - método para detectar a presença de bactérias produtoras de carbapenemase em uma amostra - Google Patents

método para detectar a presença de bactérias produtoras de carbapenemase em uma amostra

Info

Publication number
BR112013033082A2
BR112013033082A2 BR112013033082A BR112013033082A BR112013033082A2 BR 112013033082 A2 BR112013033082 A2 BR 112013033082A2 BR 112013033082 A BR112013033082 A BR 112013033082A BR 112013033082 A BR112013033082 A BR 112013033082A BR 112013033082 A2 BR112013033082 A2 BR 112013033082A2
Authority
BR
Brazil
Prior art keywords
detecting
sample
carbapenemase
producing bacteria
reagent kit
Prior art date
Application number
BR112013033082A
Other languages
English (en)
Other versions
BR112013033082B1 (pt
Inventor
Dortet Laurent
Poirel Laurent
Nordmann Patrice
Original Assignee
Hopitaux Paris Assist Publique
Inst Nat Sante Rech Med
Univ Paris Sud 11
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44904645&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112013033082(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hopitaux Paris Assist Publique, Inst Nat Sante Rech Med, Univ Paris Sud 11 filed Critical Hopitaux Paris Assist Publique
Publication of BR112013033082A2 publication Critical patent/BR112013033082A2/pt
Publication of BR112013033082B1 publication Critical patent/BR112013033082B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

resumo da patente de invenção para: “método para detectar a presença de bactérias produtoras de carbapenemase em uma amostra”. a presente invenção se refere a um método para detectar a presença de bactérias produtoras de carbapenemase em uma amostra, o método compreende as etapas de: a) realizar a lise das células em uma amostra de teste, a fim de obter uma suspensão enzimática, b) reagir uma fração da suspensão enzimática obtida na etapa a) com um kit de reagentes, o referido kit de reagente compreendendo: - um substrato de carbapenemase selecionado a partir do grupo consistindo de carbapenemos e cefamicinas, - um indicador de cor de ph, o qual vai mudar de cor quando o ph da mistura reacional é compreendido entre 6,4 e 8,4, em que a mudança de cor após a etapa b) indica a presença de bactérias produtoras de carbapenemase na amostra de teste. a invenção também se refere a um kit de reagentes, para uma placa de microtitulação e as suas utilizações na detecção da presença de produtores de carbapenemase em uma amostra de teste.
BR112013033082-1A 2011-06-22 2012-06-21 Método para detectar a presença de bactérias produtoras de carbapenemase em uma amostra BR112013033082B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161499813P 2011-06-22 2011-06-22
EP11305790 2011-06-22
US61/499,813 2011-06-22
EP11305790.5 2011-06-22
PCT/EP2012/062028 WO2012175637A1 (en) 2011-06-22 2012-06-21 Method for detecting the presence of carbapenemase-producing bacteria in a sample

Publications (2)

Publication Number Publication Date
BR112013033082A2 true BR112013033082A2 (pt) 2017-08-08
BR112013033082B1 BR112013033082B1 (pt) 2020-03-31

Family

ID=44904645

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013033082-1A BR112013033082B1 (pt) 2011-06-22 2012-06-21 Método para detectar a presença de bactérias produtoras de carbapenemase em uma amostra

Country Status (8)

Country Link
US (1) US9340819B2 (pt)
EP (1) EP2723884B1 (pt)
JP (1) JP6101257B2 (pt)
CN (2) CN103764838B (pt)
BR (1) BR112013033082B1 (pt)
CA (1) CA2837246C (pt)
DK (1) DK2723884T3 (pt)
WO (1) WO2012175637A1 (pt)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9481903B2 (en) 2013-03-13 2016-11-01 Roche Molecular Systems, Inc. Systems and methods for detection of cells using engineered transduction particles
SG11201507463WA (en) 2013-03-13 2015-10-29 Geneweave Biosciences Inc Non-replicative transduction particles and transduction particle-based reporter systems
JP6214188B2 (ja) * 2013-04-05 2017-10-18 栄研化学株式会社 β−ラクタマーゼ検出方法
EP3875912B1 (en) 2013-08-16 2023-12-20 Siemens Healthcare Diagnostics Inc. User interface tool kit for mobile devices
US9540675B2 (en) 2013-10-29 2017-01-10 GeneWeave Biosciences, Inc. Reagent cartridge and methods for detection of cells
EP3071965A1 (en) 2013-11-21 2016-09-28 Avails Medical, Inc. Electrical biosensor for detecting a substance in a bodily fluid, and method and system for same
US10041105B2 (en) 2014-04-15 2018-08-07 Cellex, Incorporated Methods and devices for detection of resistance to an enzyme inhibitor
FR3034523B1 (fr) * 2015-04-03 2020-01-17 Universite De Bourgogne Nouvelle methode pour detecter la presence de bacteries productrices de beta-lactamases.
EP4368991A2 (en) 2015-08-25 2024-05-15 Avails Medical, Inc. Devices, systems and methods for detecting viable microorganisms in a fluid sample
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells
EP3153589A1 (en) 2015-10-09 2017-04-12 Université de Fribourg Kit and methods for the rapid detection of the absence or presence of resistance to beta-lactam compounds in urine samples
GB201520977D0 (en) * 2015-11-27 2016-01-13 Mast Group Ltd Carbapenemase-producing bacteria detection method
US10254245B2 (en) 2016-01-25 2019-04-09 Avails Medical, Inc. Devices, systems and methods for detecting viable infectious agents in a fluid sample using an electrolyte-insulator-semiconductor sensor
US10174356B2 (en) 2016-05-31 2019-01-08 Avails Medical, Inc. Devices, systems and methods to detect viable infectious agents in a fluid sample and susceptibility of infectious agents to anti-infectives
CN109642249B (zh) * 2016-06-27 2023-07-18 贝克顿迪金森公司 用于检测细菌中的抗微生物抗性的组合物、方法、系统和/或试剂盒
WO2018111234A1 (en) * 2016-12-13 2018-06-21 Avails Medical, Inc. DEVICES, SYSTEMS AND METHODS TO DETECT THE PRESENCE OF ß-LACTAM ANTIBIOTIC HYDROLYZING BACTERIA IN A SAMPLE
DE102017004606B3 (de) 2017-05-12 2018-11-15 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtung zum phänotypischen Nachweis von Carbapenemase und Carbapenemase-Bildnern
WO2019005296A1 (en) 2017-06-27 2019-01-03 Avails Medical, Inc. APPARATUS, SYSTEMS AND METHODS FOR DETERMINING THE SENSITIVITY OF MICROORGANISMS TO ANTI-INFECTIOUS
GB201712671D0 (en) * 2017-08-07 2017-09-20 Mast Group Ltd Chromogenic Carbapenemase detection method
US11492653B2 (en) 2017-08-14 2022-11-08 University Of Louisville Research Foundation, Inc. Methods and kits for detecting antibiotic-inactivating factors
WO2019070739A1 (en) 2017-10-03 2019-04-11 Avails Medical, Inc. APPARATUSES, SYSTEMS AND METHODS FOR DETERMINING THE CONCENTRATION OF MICROORGANISMS AND THE SENSITIVITY OF MICROORGANISMS TO ANTI-INFECTIOUS, BASED ON OXIDOREDUCTION REACTIONS
CN109722466B (zh) * 2019-01-30 2022-03-25 华南农业大学 一种运用ai模型快速检测产生碳青霉烯酶菌株的方法
CN110438214A (zh) * 2019-08-08 2019-11-12 皖南医学院弋矶山医院 Cre菌株碳青霉烯酶检测方法的性能评估方法
CN110904199A (zh) * 2019-11-22 2020-03-24 南方医科大学南方医院 检测碳青霉烯酶blaKPC基因的引物、方法及试剂盒
CN113249434A (zh) * 2021-05-07 2021-08-13 山西大学 同时检测产超广谱β-内酰胺酶和碳青霉烯酶细菌的方法
CN114487404B (zh) * 2021-12-28 2023-06-23 中国农业大学 用于检测细菌内碳青霉烯酶的免疫层析试纸条及检测方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696239B1 (en) * 2000-04-20 2004-02-24 Biolog, Inc. Comparative phenotype analysis for assessment of biological active compounds such as antimicrobials
US8097434B2 (en) * 2007-10-19 2012-01-17 Becton, Dickinson And Company Methods for the detection of beta-lactamases
US9303282B2 (en) 2008-07-21 2016-04-05 Alain Rambach Selective enrichment medium for carbapenem-resistant bacteria

Also Published As

Publication number Publication date
CN103764838A (zh) 2014-04-30
WO2012175637A1 (en) 2012-12-27
CN105861630A (zh) 2016-08-17
DK2723884T3 (en) 2016-12-05
CN103764838B (zh) 2018-07-27
EP2723884B1 (en) 2016-08-17
US9340819B2 (en) 2016-05-17
EP2723884A1 (en) 2014-04-30
JP6101257B2 (ja) 2017-03-22
BR112013033082B1 (pt) 2020-03-31
CN105861630B (zh) 2020-01-21
JP2014519833A (ja) 2014-08-21
US20140134656A1 (en) 2014-05-15
CA2837246C (en) 2020-08-18
CA2837246A1 (en) 2012-12-27

Similar Documents

Publication Publication Date Title
BR112013033082A2 (pt) método para detectar a presença de bactérias produtoras de carbapenemase em uma amostra
BR112014012061A2 (pt) Método para detectar a presença de bactérias de produção de beta-lactamase de espectro expandido em uma amostra
EP4249605A3 (en) Methods for analyte detection
BRPI0612967B8 (pt) método para a determinação in vitro da atividade de trombina em uma amostra biológica e kit
ES2533561T3 (es) Mutantes de O-fosfoserina sulfhidrilasa y procedimiento de producción de cisteína usando los mismos
CL2008003629A1 (es) Metodo de analisis microbiologico de muestras biologicas que comprende un primer intervalo de tiempo de incubacion, tras el cual las muestras que dividen en 3 categorias (positivas, negativas o inciertas) dependiendo de la cantidad de dioxido de carbono generado, y donde las muestra que presentan un contenido de dioxido de carbono comprendido entre un valor maximo y minimo (muestras inciertas), se someten a un segundo intervalo de tiempo de incubacion y asi categorizarlas como positivas o negativas.
BR112012030788B8 (pt) Anticorpo monoclonal, linhagem celular de hibridoma, e métodos para identificar a presença e para determinação quantitativa de uma enzima aad-12
BR112014030298A2 (pt) ensaios multiplexados baseados em aptâmero
BR112013003763A2 (pt) métodos e meios para a caracterização da resistência a antibióticos em microrganismos
WO2012021795A3 (en) Pancreatic cancer biomarkers and uses thereof
EP2987866A3 (en) Method and kit for super-high-sensitivity measurement of protein and nucleic acid, and novel enzyme substrate
BRPI0607912A2 (pt) ensaio para determinar a presença, ausência ou saúde de uma célula, analito, ácido nucléico ou microorganismo em uma amostra, sonda, métodos para selecionar um analito que se ligue a um composto, para determinar se um ácido nucléico de interesse interage com uma proteína de interesse em uma célula ou uma amostra invitro, e para determinar a presença ou ausência de um ou mais compostos alvo em uma amostra, kit para manchar células, analitos, ácidos nucléicos ou microorganismos em uma amostra, e, composto"
BR112013006444A8 (pt) usos de composição de coagulação, recipientes para a preparação de amostra de soro, métodos de preparação de amostra de soro, de detecção de analito de interesse e para diagnosticar a presença, a ausência ou a gravidade de doença ou condição num sujeito e amostras de soro
HK1126274A1 (en) T cell assays t
CN1849513A (zh) 用于测定高半胱氨酸的方法和组合物
NZ720529A (en) Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
BR112013032354A2 (pt) Composições e métodos para diagnóstico e monitoração de hipertireoidismo em um felino
WO2009129173A3 (en) Methods, antibodies and kits for detecting cerebrospinal fluid in a sample
WO2011143575A3 (en) smFRET WITH MEMBRANE PROTEINS
JP2013516174A5 (pt)
MX2013004230A (es) Uso de compuestos mejoradores de señal en deteccion de electroquimioluminiscencia.
ES2530432T3 (es) Ensayo de acetaminofeno
ZA202205645B (en) Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment
WO2014008167A3 (en) Cell-based assays for post-translational enzyme activity
BR112013027041A2 (pt) Método de detecção de um analito e kit

Legal Events

Date Code Title Description
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 21/06/2012, OBSERVADAS AS CONDICOES LEGAIS.

B25G Requested change of headquarter approved

Owner name: INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE) (FR) ; ASSISTANCE PUBLIQUE HOPITAUX DE PARIS (FR) ; UNIVERSITE PARIS SUD (PARIS 11) (FR)

B25G Requested change of headquarter approved

Owner name: INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE) (FR) ; ASSISTANCE PUBLIQUE HOPITAUX DE PARIS (FR) ; UNIVERSITE PARIS SUD (PARIS 11) (FR)

B25L Entry of change of name and/or headquarter and transfer of application, patent and certificate of addition of invention: publication cancelled

Owner name: INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE) (FR) ; ASSISTANCE PUBLIQUE HOPITAUX DE PARIS (FR) ; UNIVERSITE PARIS-SACLAY (FR)

Free format text: ANULADA A PUBLICACAO CODIGO 25.7 NA RPI NO 2743 DE 01/08/2023 POR TER SIDO INDEVIDA.

B25A Requested transfer of rights approved

Owner name: INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE) (FR) ; ASSISTANCE PUBLIQUE HOPITAUX DE PARIS (FR) ; UNIVERSITE PARIS-SACLAY (FR)