BR112012030788B8 - Anticorpo monoclonal, linhagem celular de hibridoma, e métodos para identificar a presença e para determinação quantitativa de uma enzima aad-12 - Google Patents

Anticorpo monoclonal, linhagem celular de hibridoma, e métodos para identificar a presença e para determinação quantitativa de uma enzima aad-12

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Publication number
BR112012030788B8
BR112012030788B8 BR112012030788A BR112012030788A BR112012030788B8 BR 112012030788 B8 BR112012030788 B8 BR 112012030788B8 BR 112012030788 A BR112012030788 A BR 112012030788A BR 112012030788 A BR112012030788 A BR 112012030788A BR 112012030788 B8 BR112012030788 B8 BR 112012030788B8
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BR
Brazil
Prior art keywords
methods
enzyme
aad
identifying
cell line
Prior art date
Application number
BR112012030788A
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English (en)
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BR112012030788A2 (pt
BR112012030788B1 (pt
Inventor
Shan Guomin
Lin Gaofeng
K Smith-Drake Joelene
J Sosa Marcelo
Original Assignee
Dow Agrosciences Llc
Corteva Agriscience Llc
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Application filed by Dow Agrosciences Llc, Corteva Agriscience Llc filed Critical Dow Agrosciences Llc
Publication of BR112012030788A2 publication Critical patent/BR112012030788A2/pt
Publication of BR112012030788B1 publication Critical patent/BR112012030788B1/pt
Publication of BR112012030788B8 publication Critical patent/BR112012030788B8/pt

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MÉTODOS DE DETECÇÃO DE ANTICORPOS MONOCLONAIS PARA ENZIMAS QUE CONFEREM RESISTÊNCIA AO ÁCIDO 2,4-DICLOROFENOXIACÉTICO EM PLANTAS. A presente invenção refere-se a anticorpos monoclonais e métodos úteis para determinar e quantificar a presença de uma enzima ariloxialcanoato dioxigenase.
BR112012030788A 2010-06-04 2011-06-02 Anticorpo monoclonal, linhagem celular de hibridoma, e métodos para identificar a presença e para determinação quantitativa de uma enzima aad-12 BR112012030788B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35159310P 2010-06-04 2010-06-04
US61/351,593 2010-06-04
PCT/US2011/038848 WO2011153300A2 (en) 2010-06-04 2011-06-02 Monoclonal antibodies detection methods for enzymes that confer resistance to 2,4-dichlorophenoxyacetic acid in plants

Publications (3)

Publication Number Publication Date
BR112012030788A2 BR112012030788A2 (pt) 2016-10-04
BR112012030788B1 BR112012030788B1 (pt) 2020-05-12
BR112012030788B8 true BR112012030788B8 (pt) 2022-10-11

Family

ID=45064753

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BR112012030788A BR112012030788B8 (pt) 2010-06-04 2011-06-02 Anticorpo monoclonal, linhagem celular de hibridoma, e métodos para identificar a presença e para determinação quantitativa de uma enzima aad-12

Country Status (12)

Country Link
US (1) US8460891B2 (pt)
EP (1) EP2576623B1 (pt)
JP (3) JP5978205B2 (pt)
KR (1) KR101863384B1 (pt)
CN (1) CN102985442B (pt)
AU (1) AU2011261411B2 (pt)
BR (1) BR112012030788B8 (pt)
CA (1) CA2799439C (pt)
CL (1) CL2012003375A1 (pt)
DK (1) DK2576623T3 (pt)
MX (1) MX2012014152A (pt)
WO (1) WO2011153300A2 (pt)

Families Citing this family (373)

* Cited by examiner, † Cited by third party
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CN103209993B (zh) 2010-09-15 2014-12-10 陶氏益农公司 针对赋予对2,4-二氯苯氧乙酸的抗性的酶的单克隆抗体和检测方法
WO2014100388A1 (en) 2012-12-20 2014-06-26 Dow Agrosciences Llc Herbicidal compositions comprising fluroxypyr and flumetsulam
CN103823067A (zh) * 2014-03-17 2014-05-28 河南工业大学 2,4-二氯苯氧乙酸酶联免疫检测试剂盒
EP3137489A4 (en) * 2014-04-29 2017-09-20 Dow AgroSciences LLC Monoclonal antibodies specific for cry1ca and related detection methods
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