MX2009010492A - Elementos de vector de expresion recombinante (reves) para mejorar la expresion de proteinas recombinantes en celulas huesped. - Google Patents

Elementos de vector de expresion recombinante (reves) para mejorar la expresion de proteinas recombinantes en celulas huesped.

Info

Publication number
MX2009010492A
MX2009010492A MX2009010492A MX2009010492A MX2009010492A MX 2009010492 A MX2009010492 A MX 2009010492A MX 2009010492 A MX2009010492 A MX 2009010492A MX 2009010492 A MX2009010492 A MX 2009010492A MX 2009010492 A MX2009010492 A MX 2009010492A
Authority
MX
Mexico
Prior art keywords
recombinant
reves
expression
vector elements
host cells
Prior art date
Application number
MX2009010492A
Other languages
English (en)
Inventor
Gerald R Carson
Wendy R Gion
Hong Gao
Yune Z Kunes
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Publication of MX2009010492A publication Critical patent/MX2009010492A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • C07K16/2854—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72 against selectins, e.g. CD62
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se describen composiciones y métodos que comprenden elementos de vector de expresión recombinante (rEVEs) para mejorar el nivel de expresión de proteínas recombinantes. También se describen otras composiciones y métodos para reducir, suprimir substancialmente, o silenciar esencialmente la expresión de una proteína recombinante.
MX2009010492A 2007-03-30 2008-03-28 Elementos de vector de expresion recombinante (reves) para mejorar la expresion de proteinas recombinantes en celulas huesped. MX2009010492A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92114107P 2007-03-30 2007-03-30
PCT/US2008/004063 WO2008121324A2 (en) 2007-03-30 2008-03-28 Recombinant expression vector elements (reves) for enhancing expression of recombinant proteins in host cells

Publications (1)

Publication Number Publication Date
MX2009010492A true MX2009010492A (es) 2009-10-19

Family

ID=39795081

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009010492A MX2009010492A (es) 2007-03-30 2008-03-28 Elementos de vector de expresion recombinante (reves) para mejorar la expresion de proteinas recombinantes en celulas huesped.

Country Status (16)

Country Link
US (2) US7935808B2 (es)
EP (1) EP2142560A4 (es)
JP (2) JP5432117B2 (es)
KR (2) KR20100014724A (es)
CN (1) CN101652379B (es)
AU (1) AU2008233196B2 (es)
BR (1) BRPI0809361A2 (es)
CA (1) CA2681581A1 (es)
IL (1) IL201231A0 (es)
MX (1) MX2009010492A (es)
NZ (1) NZ580378A (es)
RU (1) RU2518340C2 (es)
SG (1) SG182953A1 (es)
TW (1) TW200907059A (es)
WO (1) WO2008121324A2 (es)
ZA (1) ZA200906433B (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100014724A (ko) * 2007-03-30 2010-02-10 아보트 러보러터리즈 숙주 세포에서 재조합 단백질의 발현을 증강시키기 위한 재조합 발현 벡터 요소(ｒＥＶＥ)
BRPI0920027A2 (pt) * 2008-10-20 2015-10-06 Abbott Lab isolamento e purificação de anticorpos usando cromatografia de afinidade a proteína a
ES2440321T3 (es) 2009-02-27 2014-01-28 Novartis Ag Métodos para seleccionar células eucarióticas que expresan una proteína heteróloga
IN2012DN02304A (es) * 2009-08-17 2015-08-21 Nox Technologies Inc
US20110150861A1 (en) 2009-10-30 2011-06-23 Abbott Laboratories Sorf constructs and multiple gene expression
SG11201403223PA (en) 2011-12-22 2014-07-30 Hoffmann La Roche Expression vector organization, novel production cell generation methods and their use for the recombinant production of polypeptides
KR102229491B1 (ko) * 2011-12-22 2021-03-18 에프. 호프만-라 로슈 아게 발현 벡터 구성원 조합, 신규한 제조 세포 생성 방법 및 폴리펩티드의 재조합적 제조를 위한 그의 용도
US9752159B2 (en) * 2012-03-30 2017-09-05 Biogenomics Limited Stable expression system for eukaryotic cells
KR102073748B1 (ko) * 2013-01-31 2020-02-05 한미약품 주식회사 재조합 효모 형질전환체 및 이를 이용하여 면역글로불린 단편을 생산하는 방법
WO2015017823A1 (en) * 2013-08-02 2015-02-05 The Uab Research Foundation Tetravalent pneumococcal serogroup 6z
RU2546249C2 (ru) 2013-08-07 2015-04-10 Силезиа Груп Пте. Лтд. Композиция для усиления экспрессии трансгена в эукариотических клетках и способ увеличения продукции целевого белка, кодируемого трансгеном
EP3057616B1 (en) 2013-10-16 2020-03-11 Outlook Therapeutics, Inc. Buffer formulations for enhanced antibody stability
EP3247718B1 (en) 2015-01-21 2021-09-01 Outlook Therapeutics, Inc. Modulation of charge variants in a monoclonal antibody composition
WO2016151599A1 (en) 2015-03-25 2016-09-29 Biogenomics Limited A bigenic mammalian expression vector
WO2017136433A1 (en) 2016-02-03 2017-08-10 Oncobiologics, Inc. Buffer formulations for enhanced antibody stability
WO2019055471A1 (en) * 2017-09-18 2019-03-21 Amyris, Inc. METHODS FOR PRODUCING FULL LENGTH ANTIBODIES
US20210261649A1 (en) * 2018-06-29 2021-08-26 Krystal Biotech, Inc, Compositions and methods for antibody delivery
PT115073A (pt) * 2018-10-09 2020-05-29 Inst De Biologia Molecular E Celular Ibmc Ácido nucleico para ativar a expressão e a produção de proteínas
CN110592090A (zh) * 2019-10-30 2019-12-20 福建师范大学 Ssa4基因启动子及利用该启动子驱动外源基因转录的毕赤酵母表达载体

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE68913658T3 (de) 1988-11-11 2005-07-21 Stratagene, La Jolla Klonierung von Immunglobulin Sequenzen aus den variablen Domänen
US6596514B2 (en) 1996-01-11 2003-07-22 Immunex Corporation Expression augmenting sequence elements (EASE) for eukaryotic expression systems
PT873405E (pt) 1996-01-11 2005-01-31 Immunex Corp Elementos de sequencia que aumentam a expressao (ease) para sistemas de expressao eucarioticos
US6197591B1 (en) * 1998-09-14 2001-03-06 Pfizer Inc. Streptomyces avermitilis regulatory genes for increased avermectin production
DE60233049D1 (de) 2001-01-26 2009-09-03 Selexis Sa Matrix-anheftungsregionen und verfahren zu deren verwendung
WO2004033693A1 (en) * 2002-03-29 2004-04-22 Xoma Technology Ltd. Methods vectors plasmids and methods for increasing expression of recombinant polypeptides
US7326567B2 (en) * 2003-11-12 2008-02-05 Schering Corporation Plasmid system for multigene expression
TWI323734B (en) 2005-08-19 2010-04-21 Abbott Lab Dual variable domain immunoglobulin and uses thereof
DK3339445T3 (da) * 2006-09-08 2020-10-19 Abbvie Bahamas Ltd Interleukin -13 bindende proteiner
KR20100014724A (ko) * 2007-03-30 2010-02-10 아보트 러보러터리즈 숙주 세포에서 재조합 단백질의 발현을 증강시키기 위한 재조합 발현 벡터 요소(ｒＥＶＥ)

Also Published As

Publication number Publication date
RU2518340C2 (ru) 2014-06-10
NZ580378A (en) 2012-11-30
CN101652379A (zh) 2010-02-17
US8410259B2 (en) 2013-04-02
EP2142560A4 (en) 2010-12-01
US20110201053A1 (en) 2011-08-18
RU2009140146A (ru) 2011-05-10
AU2008233196B2 (en) 2011-10-13
JP2010523082A (ja) 2010-07-15
JP2014012014A (ja) 2014-01-23
KR20140001229A (ko) 2014-01-06
WO2008121324A3 (en) 2008-12-11
SG182953A1 (en) 2012-08-30
ZA200906433B (en) 2014-03-26
WO2008121324A2 (en) 2008-10-09
AU2008233196A1 (en) 2008-10-09
US7935808B2 (en) 2011-05-03
IL201231A0 (en) 2010-05-31
US20080241883A1 (en) 2008-10-02
CA2681581A1 (en) 2008-10-09
EP2142560A2 (en) 2010-01-13
CN101652379B (zh) 2014-05-14
JP5432117B2 (ja) 2014-03-05
BRPI0809361A2 (pt) 2014-09-02
TW200907059A (en) 2009-02-16
KR20100014724A (ko) 2010-02-10

Similar Documents

Publication Publication Date Title
MX2009010492A (es) Elementos de vector de expresion recombinante (reves) para mejorar la expresion de proteinas recombinantes en celulas huesped.
CY1122672T1 (el) Βελτιωμενα nanobodiestm εναντι του παραγοντα νεκρωσης ογκων αλφα
PT1991680E (pt) Sistema para a expressão de componentes ortogonais de tradução em células hospedeiras eubacterianas
EA200801531A1 (ru) Способы регулирования содержания маннозы в рекомбинантных белках
MY169472A (en) Refolding of recombinant proteins
WO2009137778A3 (en) Methods and compositions for genetically engineering clostridia species
MY174493A (en) Binding agents
MX2007013759A (es) Agentes de union de esclerostina.
WO2009147248A3 (en) Amino acid sequences directed against envelope proteins of a virus and polypeptides comprising the same for the treatment of viral diseases
WO2006078645A3 (en) Heterologous polypeptide expression using low multiplicity of infection of viruses
EP2598660A4 (en) COMPOSITIONS FOR STABILIZING DNA, RNA AND PROTEINS IN BLOOD AND OTHER BIODEGRADS DURING TRANSPORT AND STORAGE IN AMBIENT TEMPERATURES
WO2008083271A3 (en) Methods and compositions for enhanced protein expression and purification
NO20065084L (no) Interferon-alfa polypeptider og konjugater
WO2007092704A8 (en) Genes for enhancing nitrogen utilization efficiency in crop plants
EP2470559A4 (en) COAGULATION FACTOR IX COMPOSITIONS AND METHODS OF MAKING AND USING THEM
ES2353814T3 (es) Proteinas n-glicosiladas recombinantes de celulas procariotas.
EP2598661A4 (en) COMPOSITIONS FOR STABILIZING DNA, RNA AND PROTEINS IN STORAGE AND OTHER BIODEGRADES DURING TRANSPORT AND STORAGE IN AMBIENT TEMPERATURES
WO2008104890A3 (en) Compositions and methods for producing apolipoprotein
ZA200800517B (en) Transplastomic plants expressing lumen-targeted protein
DK2089516T3 (da) Fremgangsmåder til at forbedre indføringen af DNA i bakterielle celler
EP1963506A4 (en) COMPOSITIONS AND METHODS FOR INCREASING THE PRODUCTION OF RECOMBINANT GAMMA-CARBOXYLATED PROTEINS
WO2010056144A3 (en) Foxp3+ natural kiler t-cells and the treatment of immune related diseases
WO2007134325A3 (en) Methods and compositions for protein production using adenoviral vectors
IN2012DN00448A (es)
MD20130099A2 (ro) Expresia recombinantă a interferonului solubil

Legal Events

Date Code Title Description
FG Grant or registration
GB Transfer or rights