LT4001B - Process for preparing transformed prokaryotic host cell, stable gene amplification in the chromosome dna of prokaryotic microorganisms - Google Patents

Process for preparing transformed prokaryotic host cell, stable gene amplification in the chromosome dna of prokaryotic microorganisms Download PDF

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Publication number
LT4001B
LT4001B LTIP1826A LTIP1826A LT4001B LT 4001 B LT4001 B LT 4001B LT IP1826 A LTIP1826 A LT IP1826A LT IP1826 A LTIP1826 A LT IP1826A LT 4001 B LT4001 B LT 4001B
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LT
Lithuania
Prior art keywords
dna
bacillus
strain
recipient
chromosome
Prior art date
Application number
LTIP1826A
Other languages
English (en)
Lithuanian (lt)
Inventor
Christiaan Albertus Vaneekelen
Johannes Cornelis Vanderlaan
Leonardus Johannes S Mulleners
Original Assignee
Gist Brocades Nv
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8197582&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=LT4001(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Publication of LTIP1826A publication Critical patent/LTIP1826A/xx
Publication of LT4001B publication Critical patent/LT4001B/lt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
LTIP1826A 1987-02-27 1994-01-28 Process for preparing transformed prokaryotic host cell, stable gene amplification in the chromosome dna of prokaryotic microorganisms LT4001B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP87200356 1987-02-27

Publications (2)

Publication Number Publication Date
LTIP1826A LTIP1826A (en) 1995-08-25
LT4001B true LT4001B (en) 1996-06-25

Family

ID=8197582

Family Applications (1)

Application Number Title Priority Date Filing Date
LTIP1826A LT4001B (en) 1987-02-27 1994-01-28 Process for preparing transformed prokaryotic host cell, stable gene amplification in the chromosome dna of prokaryotic microorganisms

Country Status (20)

Country Link
US (2) US5733723A (hu)
EP (1) EP0284126B1 (hu)
JP (1) JP2637532B2 (hu)
KR (1) KR970000188B1 (hu)
CN (1) CN1049247C (hu)
AU (1) AU620326B2 (hu)
BG (1) BG60920B2 (hu)
BR (1) BR8805646A (hu)
CA (1) CA1327175C (hu)
DE (1) DE3883041T2 (hu)
ES (1) ES2045081T3 (hu)
FI (1) FI104326B (hu)
HU (1) HUT50877A (hu)
IE (1) IE61743B1 (hu)
LT (1) LT4001B (hu)
LV (1) LV10791B (hu)
NO (1) NO300382B1 (hu)
PT (1) PT86863B (hu)
RU (1) RU2091487C1 (hu)
WO (1) WO1988006623A1 (hu)

Families Citing this family (34)

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BR8805646A (pt) * 1987-02-27 1989-10-17 Gist Brocades Nv Processo para amplificacao de genes estaveis em adn cromossomico de microorganismos procarioticos
EP0479396B1 (en) * 1987-02-27 1999-06-09 Genencor International, Inc. Transformation of alkalophilic bacillus strains
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
DK0414297T3 (da) * 1989-08-11 1997-07-07 Genencor Int Effektiv fremstilling af mutantproteaser
US5695976A (en) * 1989-12-18 1997-12-09 Novo Nordisk A/S Stable integration of DNA in bacterial genomes
JP3046344B2 (ja) * 1990-10-24 2000-05-29 塩野義製薬株式会社 新規プロテアーゼ
JPH07503363A (ja) * 1991-11-14 1995-04-13 ノボザイムス アクティーゼルスカブ バシラス・リヘニフォルミス中で遺伝子を発現させる方法
DE4216246A1 (de) * 1992-05-16 1993-11-18 Solvay Enzymes Gmbh & Co Kg Einfaches Verfahren zur stabilen chromosomalen Genamplifikation
US5395763A (en) * 1992-06-24 1995-03-07 Monsanto Company Chromosomal expression vector
DK153992D0 (da) * 1992-12-22 1992-12-22 Novo Nordisk As Metode
US5861273A (en) * 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
US6723550B1 (en) 1997-07-15 2004-04-20 Genencor International, Inc. Proteases from gram-positive organisms
GB9724627D0 (en) 1997-11-20 1998-01-21 Genencor Int Bv Gram positive microorganism formate pathway
US6528255B1 (en) 1997-12-30 2003-03-04 Genencor International, Inc. Proteases from gram positive organisms
US6465186B1 (en) 1997-12-30 2002-10-15 Genecor International, Inc. Proteases from gram positive organisms
US6599731B1 (en) 1997-12-30 2003-07-29 Genencor International, Inc. Proteases from gram positive organisms
GB9727470D0 (en) 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
US6100063A (en) * 1998-02-12 2000-08-08 Novo Nordisk A/S Procaryotic cell comprising at least two copies of a gene
WO1999041358A1 (en) * 1998-02-12 1999-08-19 Novo Nordisk A/S A prokaryotic cell comprising two copies of a gene transcribed in different directions
US6156514A (en) * 1998-12-03 2000-12-05 Sunol Molecular Corporation Methods for making recombinant cells
US6544792B1 (en) 1999-12-21 2003-04-08 Genencor International, Inc. Production of secreted polypeptides
AU2001265835A1 (en) * 2000-06-23 2002-01-08 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
BRPI0416797A (pt) 2003-11-19 2007-04-17 Genencor Int serina proteases, ácidos nucléicos codificando enzimas de serina e vetores e células hospedeiras incorporando as mesmas
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
US20050227356A1 (en) * 2004-04-07 2005-10-13 Lessard Philip A Novel compositions and methods for genetic manipulation of Rhodococcus bacteria
US7842479B2 (en) * 2004-05-21 2010-11-30 The Board Of Regents Of The University Of Nebraska Methods for altering acetic acid production and enhancing cell death in bacteria
US20080044853A1 (en) * 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
ATE443128T1 (de) 2004-10-22 2009-10-15 Novozymes As Stabile genomische integration mehrerer polynukleotidkopien
DE102007021001A1 (de) 2007-05-04 2008-11-06 Ab Enzymes Gmbh Expressionssystem zur antibiotikafreien Produktion von Polypeptiden
CN103290048B (zh) * 2013-06-27 2015-07-15 天津科技大学 一种适用于芽孢杆菌属同源重组的质粒及工程菌
ES2855992T3 (es) 2015-12-11 2021-09-27 Wacker Chemie Ag Cepa de microorganismos y procedimiento para la producción fermentativa exenta de antibióticos de sustancias y proteínas de bajo peso molecular
CN114032189B (zh) * 2021-05-10 2024-02-06 盐城师范学院 一种降解木质素的融合子菌株r3及其应用
GB202304540D0 (en) 2023-03-28 2023-05-10 Univ Tartu Method of biosynthesis

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0134048A1 (en) 1983-07-06 1985-03-13 Gist-Brocades N.V. Molecular cloning and expression in industrial microorganism species

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IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
EP0074553A3 (en) * 1981-09-11 1985-01-09 The University Of Rochester Method of increasing the yield of a product by altering a microorganism
JPS59205983A (ja) * 1983-04-28 1984-11-21 ジエネツクス・コ−ポレイシヨン 異種遺伝子を原核微生物で発現させる方法
US4617266A (en) * 1983-04-28 1986-10-14 Genex Corporation Production of Protein A
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FR2563533B1 (fr) * 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
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Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
EP0134048A1 (en) 1983-07-06 1985-03-13 Gist-Brocades N.V. Molecular cloning and expression in industrial microorganism species

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DEBADO: "Bacilų molekulinė biologija"
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FERRARI E. ET AL.: "Isolation of an Alanine Racemase Gene from Bacillus subtilis and its Use for Plasmid Maintenance in B. subtilis", NATURE BIOTECHNOLOGY, 1985, pages 1003 - 1007
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Also Published As

Publication number Publication date
LV10791A (lv) 1995-08-20
NO884422L (no) 1988-10-05
PT86863B (pt) 1992-05-29
FI104326B1 (fi) 1999-12-31
JPH01502398A (ja) 1989-08-24
BR8805646A (pt) 1989-10-17
KR970000188B1 (en) 1997-01-06
HUT50877A (en) 1990-03-28
EP0284126B1 (en) 1993-08-11
KR890700664A (ko) 1989-04-26
AU620326B2 (en) 1992-02-20
JP2637532B2 (ja) 1997-08-06
PT86863A (pt) 1988-03-01
LV10791B (en) 1995-12-20
NO300382B1 (no) 1997-05-20
CN1049247C (zh) 2000-02-09
IE61743B1 (en) 1994-11-30
DE3883041T2 (de) 1994-01-20
US6124097A (en) 2000-09-26
AU1398188A (en) 1988-09-26
ES2045081T3 (es) 1994-01-16
EP0284126A1 (en) 1988-09-28
US5733723A (en) 1998-03-31
LTIP1826A (en) 1995-08-25
CN1030787A (zh) 1989-02-01
WO1988006623A1 (en) 1988-09-07
NO884422D0 (no) 1988-10-05
DE3883041D1 (de) 1993-09-16
IE880558L (en) 1988-08-27
CA1327175C (en) 1994-02-22
FI884903A0 (fi) 1988-10-24
FI884903A (fi) 1988-10-24
FI104326B (fi) 1999-12-31
BG60920B2 (bg) 1996-06-28
RU2091487C1 (ru) 1997-09-27

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