ATE443128T1 - Stabile genomische integration mehrerer polynukleotidkopien - Google Patents
Stabile genomische integration mehrerer polynukleotidkopienInfo
- Publication number
- ATE443128T1 ATE443128T1 AT05794647T AT05794647T ATE443128T1 AT E443128 T1 ATE443128 T1 AT E443128T1 AT 05794647 T AT05794647 T AT 05794647T AT 05794647 T AT05794647 T AT 05794647T AT E443128 T1 ATE443128 T1 AT E443128T1
- Authority
- AT
- Austria
- Prior art keywords
- genomic integration
- multiple polynucleotide
- stable genomic
- polynucleotide copies
- copies
- Prior art date
Links
- 230000010354 integration Effects 0.000 title abstract 2
- 102000040430 polynucleotide Human genes 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 title 1
- 239000002157 polynucleotide Substances 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 3
- 108700026244 Open Reading Frames Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 108010052160 Site-specific recombinase Proteins 0.000 abstract 1
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 238000005215 recombination Methods 0.000 abstract 1
- 230000006798 recombination Effects 0.000 abstract 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Electronic Switches (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200401621 | 2004-10-22 | ||
| DKPA200401785 | 2004-11-17 | ||
| PCT/DK2005/000673 WO2006042548A1 (en) | 2004-10-22 | 2005-10-19 | Stable genomic integration of multiple polynucleotide copies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE443128T1 true ATE443128T1 (de) | 2009-10-15 |
Family
ID=35596262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05794647T ATE443128T1 (de) | 2004-10-22 | 2005-10-19 | Stabile genomische integration mehrerer polynukleotidkopien |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US20080085535A1 (de) |
| EP (1) | EP1805296B1 (de) |
| CN (1) | CN101061214B (de) |
| AT (1) | ATE443128T1 (de) |
| DE (1) | DE602005016707D1 (de) |
| DK (1) | DK1805296T3 (de) |
| WO (1) | WO2006042548A1 (de) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101006174B (zh) | 2004-06-21 | 2010-05-26 | 诺维信公司 | 以相同取向稳定保持的至少两个orf的多拷贝 |
| ATE443128T1 (de) * | 2004-10-22 | 2009-10-15 | Novozymes As | Stabile genomische integration mehrerer polynukleotidkopien |
| AU2008252990B2 (en) * | 2007-05-17 | 2015-01-15 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Method for producing a recombinant protein on a manufacturing scale |
| EP2333091B1 (de) | 2007-05-29 | 2017-06-21 | Nature Technology Corporation | Vektoren und verfahren zur genetischen immunisierung |
| WO2010024905A1 (en) * | 2008-08-27 | 2010-03-04 | Massachusetts Institute Of Technology | Genetically stabilized tandem gene duplication |
| EP2527448A1 (de) | 2011-05-23 | 2012-11-28 | Novozymes A/S | Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze |
| EP2825651B1 (de) | 2012-03-12 | 2018-04-25 | DSM IP Assets B.V. | Rekombinationssystem |
| US10793850B2 (en) | 2012-03-12 | 2020-10-06 | Dsm Ip Assets B.V. | Recombination system |
| US9631197B2 (en) * | 2012-03-12 | 2017-04-25 | Dsm Ip Assets B.V. | Rasamsonia transformants |
| JP2016521579A (ja) | 2013-06-21 | 2016-07-25 | ノボザイムス アクティーゼルスカブ | バチルス属（Ｂａｃｉｌｌｕｓ）における分泌シグナルを含まないポリペプチドの生成 |
| US11130969B2 (en) | 2013-07-12 | 2021-09-28 | Novozymes, A/S | Direct transfer of polynucleotides between genomes |
| WO2016180928A1 (en) | 2015-05-12 | 2016-11-17 | Novozymes A/S | Bacillus licheniformis host cell with deleted lantibiotic gene(s) |
| CN106906238B (zh) * | 2015-12-22 | 2020-08-14 | 中国科学院分子植物科学卓越创新中心 | 一种链霉菌抗生素生物合成基因簇多拷贝扩增方法及应用 |
| CN109804072A (zh) * | 2016-10-25 | 2019-05-24 | 诺维信公司 | 地衣芽孢杆菌中flp介导的基因组整合 |
| CN108251344A (zh) * | 2016-12-28 | 2018-07-06 | 中国科学院上海生命科学研究院 | 系列天蓝色链霉菌高效异源表达宿主的构建与应用 |
| WO2018134386A1 (en) | 2017-01-23 | 2018-07-26 | Novozymes A/S | Host cells and methods for producing double-stranded rna |
| AU2018225180B2 (en) | 2017-02-22 | 2024-09-12 | Io Biosciences, Inc. | Nucleic acid constructs comprising gene editing multi-sites and uses thereof |
| CN111417726A (zh) * | 2017-10-23 | 2020-07-14 | 诺维信公司 | 通过与前肽共表达改善蛋白酶的表达 |
| EP3707252A1 (de) | 2017-11-10 | 2020-09-16 | Novozymes A/S | Temperaturempfindliches cas9-protein |
| US20210017544A1 (en) | 2017-12-22 | 2021-01-21 | Novozymes A/S | Counter-Selection by Inhibition of Conditionally Essential Genes |
| WO2020229191A1 (en) | 2019-05-15 | 2020-11-19 | Novozymes A/S | Temperature-sensitive rna-guided endonuclease |
| EP3990629A1 (de) | 2019-06-25 | 2022-05-04 | Novozymes A/S | Gegenauswahl durch hemmung von bedingt essenziellen genen |
| EP4133086A4 (de) * | 2020-04-07 | 2024-06-05 | IO Biosciences, Inc. | Nukleinsäurekonstrukte mit geneditierenden multi-sites |
| US20240294894A1 (en) | 2021-06-24 | 2024-09-05 | Basf Se | Improved bacillus host cell with altered rema/remb protein |
| EP4370534A1 (de) | 2021-07-13 | 2024-05-22 | Novozymes A/S | Rekombinante cutinase-expression |
| US20250304952A1 (en) * | 2021-10-11 | 2025-10-02 | The Board Of Trustees Of The Leland Stanford Junior University | Dna element responsive to extrachromosomal dna in cancer cells |
| US20250034535A1 (en) | 2021-12-10 | 2025-01-30 | Novozymes A/S | Improved protein production in recombinant bacteria |
| EP4630563A1 (de) | 2022-12-05 | 2025-10-15 | Novozymes A/S | Modifizierte rna-polymeraseaktivitäten |
| WO2026017381A1 (en) | 2024-07-17 | 2026-01-22 | Novozymes A/S | Optimized bacillus host cells |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2563533B1 (fr) | 1984-04-27 | 1986-08-22 | Centre Nat Rech Scient | Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues |
| HUT50877A (en) | 1987-02-27 | 1990-03-28 | Gist Brocades Nv | Process for producing stable gene amplification in chromosomal dna of procaryote microorganisms |
| US5470727A (en) * | 1993-12-21 | 1995-11-28 | Celtrix Pharmaceuticals, Inc. | Chromosomal expression of non-bacterial genes in bacterial cells |
| US6017694A (en) * | 1997-12-19 | 2000-01-25 | American Cyanamid Company | Methods of screening for modulators of respiratory syncytial virus matrix protein interaction |
| US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| WO2000005355A1 (en) * | 1998-07-24 | 2000-02-03 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
| AU7953900A (en) * | 1999-10-29 | 2001-05-14 | Takara Shuzo Co., Ltd. | Gene transfer method |
| JP2004501651A (ja) | 2000-06-23 | 2004-01-22 | ノボザイムス アクティーゼルスカブ | 遺伝子の安定した染色体多コピー組み込みのための方法 |
| CN1455817A (zh) * | 2000-07-21 | 2003-11-12 | (由农业部部长代表的)美利坚合众国 | 用于在真核基因组中置换、转移和富积dna的方法 |
| AU2003265608A1 (en) | 2002-08-21 | 2004-03-11 | Kosan Biosciences, Inc. | Myxococcus xanthus bacteriophage mx9 transformation and integration system |
| EP1405908A1 (de) * | 2002-10-04 | 2004-04-07 | ProBioGen AG | Herstellung von Zelllinien zur heterologen Expression |
| US20040209370A1 (en) * | 2002-12-19 | 2004-10-21 | Wonchul Suh | Method for chromosomal engineering |
| ATE443128T1 (de) * | 2004-10-22 | 2009-10-15 | Novozymes As | Stabile genomische integration mehrerer polynukleotidkopien |
| EP2527448A1 (de) * | 2011-05-23 | 2012-11-28 | Novozymes A/S | Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze |
-
2005
- 2005-10-19 AT AT05794647T patent/ATE443128T1/de not_active IP Right Cessation
- 2005-10-19 WO PCT/DK2005/000673 patent/WO2006042548A1/en not_active Ceased
- 2005-10-19 DK DK05794647.7T patent/DK1805296T3/da active
- 2005-10-19 US US11/576,896 patent/US20080085535A1/en not_active Abandoned
- 2005-10-19 CN CN2005800362425A patent/CN101061214B/zh not_active Expired - Lifetime
- 2005-10-19 EP EP05794647A patent/EP1805296B1/de not_active Expired - Lifetime
- 2005-10-19 DE DE602005016707T patent/DE602005016707D1/de not_active Expired - Lifetime
-
2014
- 2014-03-20 US US14/221,005 patent/US20140273237A1/en not_active Abandoned
-
2017
- 2017-04-07 US US15/481,879 patent/US11174487B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DK1805296T3 (da) | 2010-01-18 |
| US20140273237A1 (en) | 2014-09-18 |
| CN101061214A (zh) | 2007-10-24 |
| US20170218381A1 (en) | 2017-08-03 |
| EP1805296B1 (de) | 2009-09-16 |
| US11174487B2 (en) | 2021-11-16 |
| EP1805296A1 (de) | 2007-07-11 |
| US20080085535A1 (en) | 2008-04-10 |
| DE602005016707D1 (de) | 2009-10-29 |
| CN101061214B (zh) | 2012-12-05 |
| WO2006042548A1 (en) | 2006-04-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |