DE602005016707D1 - Stabile genomische integration mehrerer polynukleotidkopien - Google Patents

Stabile genomische integration mehrerer polynukleotidkopien

Info

Publication number
DE602005016707D1
DE602005016707D1 DE602005016707T DE602005016707T DE602005016707D1 DE 602005016707 D1 DE602005016707 D1 DE 602005016707D1 DE 602005016707 T DE602005016707 T DE 602005016707T DE 602005016707 T DE602005016707 T DE 602005016707T DE 602005016707 D1 DE602005016707 D1 DE 602005016707D1
Authority
DE
Germany
Prior art keywords
genome integration
multiple polynucleotide
polynucleotide copies
stable genome
copies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005016707T
Other languages
English (en)
Inventor
Anne Breuener
Michael Dolberg Rasmussen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of DE602005016707D1 publication Critical patent/DE602005016707D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Electronic Switches (AREA)
DE602005016707T 2004-10-22 2005-10-19 Stabile genomische integration mehrerer polynukleotidkopien Active DE602005016707D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200401621 2004-10-22
DKPA200401785 2004-11-17
PCT/DK2005/000673 WO2006042548A1 (en) 2004-10-22 2005-10-19 Stable genomic integration of multiple polynucleotide copies

Publications (1)

Publication Number Publication Date
DE602005016707D1 true DE602005016707D1 (de) 2009-10-29

Family

ID=35596262

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005016707T Active DE602005016707D1 (de) 2004-10-22 2005-10-19 Stabile genomische integration mehrerer polynukleotidkopien

Country Status (7)

Country Link
US (3) US20080085535A1 (de)
EP (1) EP1805296B1 (de)
CN (1) CN101061214B (de)
AT (1) ATE443128T1 (de)
DE (1) DE602005016707D1 (de)
DK (1) DK1805296T3 (de)
WO (1) WO2006042548A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080044853A1 (en) * 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
CN101061214B (zh) * 2004-10-22 2012-12-05 诺维信公司 多个多核苷酸拷贝的稳定基因组整合
EP2160469B1 (de) * 2007-05-17 2018-02-28 Boehringer Ingelheim RCV GmbH & Co KG Verfahren zur herstellung eines rekombinanten proteins im produktionsmassstab
WO2008153733A2 (en) * 2007-05-29 2008-12-18 Nature Technology Corporation Vectors and methods for genetic immunization
US20110236927A1 (en) * 2008-08-27 2011-09-29 Massachusetts Institute Of Technology Genetically stabilized tandem gene duplication
EP2527448A1 (de) 2011-05-23 2012-11-28 Novozymes A/S Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze
BR112014022467A2 (pt) * 2012-03-12 2017-07-11 Dsm Ip Assets Bv transformantes de rasamsonia
EP2825651B1 (de) 2012-03-12 2018-04-25 DSM IP Assets B.V. Rekombinationssystem
US10793850B2 (en) 2012-03-12 2020-10-06 Dsm Ip Assets B.V. Recombination system
CN105324488A (zh) 2013-06-21 2016-02-10 诺维信公司 芽孢杆菌属中不具有分泌信号的多肽的生产
EP3019607B2 (de) 2013-07-12 2022-09-07 Novozymes A/S Direkter transport von polynucleotiden zwischen genomen
WO2016180928A1 (en) 2015-05-12 2016-11-17 Novozymes A/S Bacillus licheniformis host cell with deleted lantibiotic gene(s)
CN106906238B (zh) * 2015-12-22 2020-08-14 中国科学院分子植物科学卓越创新中心 一种链霉菌抗生素生物合成基因簇多拷贝扩增方法及应用
CN109804072A (zh) * 2016-10-25 2019-05-24 诺维信公司 地衣芽孢杆菌中flp介导的基因组整合
CN108251344A (zh) * 2016-12-28 2018-07-06 中国科学院上海生命科学研究院 系列天蓝色链霉菌高效异源表达宿主的构建与应用
WO2018134386A1 (en) 2017-01-23 2018-07-26 Novozymes A/S Host cells and methods for producing double-stranded rna
CN111417726A (zh) * 2017-10-23 2020-07-14 诺维信公司 通过与前肽共表达改善蛋白酶的表达
WO2019092042A1 (en) 2017-11-10 2019-05-16 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
US20220275354A1 (en) 2019-05-15 2022-09-01 Novozymes A/S TEMPERATURE-SENSITIVE RNA- Guided Endonuclease
EP3990629A1 (de) 2019-06-25 2022-05-04 Novozymes A/S Gegenauswahl durch hemmung von bedingt essenziellen genen
EP4133086A4 (de) * 2020-04-07 2024-06-05 IO Biosciences, Inc. Nukleinsäurekonstrukte mit geneditierenden multi-sites
WO2022269084A1 (en) 2021-06-24 2022-12-29 Basf Se Improved bacillus host cell with altered rema/remb protein
EP4370534A1 (de) 2021-07-13 2024-05-22 Novozymes A/S Rekombinante cutinase-expression
WO2023064778A1 (en) * 2021-10-11 2023-04-20 The Board Of Trustees Of The Leland Stanford Junior University Dna element responsive to extrachromosomal dna in cancer cells
WO2023104846A1 (en) 2021-12-10 2023-06-15 Novozymes A/S Improved protein production in recombinant bacteria
WO2024120767A1 (en) 2022-12-05 2024-06-13 Novozymes A/S Modified rna polymerase activities

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533B1 (fr) 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
HUT50877A (en) 1987-02-27 1990-03-28 Gist Brocades Nv Process for producing stable gene amplification in chromosomal dna of procaryote microorganisms
US5470727A (en) * 1993-12-21 1995-11-28 Celtrix Pharmaceuticals, Inc. Chromosomal expression of non-bacterial genes in bacterial cells
US6017694A (en) * 1997-12-19 2000-01-25 American Cyanamid Company Methods of screening for modulators of respiratory syncytial virus matrix protein interaction
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
AU8592298A (en) * 1998-07-24 2000-02-14 Baylor College Of Medicine Rapid subcloning using site-specific recombination
CN1183257C (zh) * 1999-10-29 2005-01-05 宝生物工程株式会社 基因转移的方法
EP1297170B1 (de) 2000-06-23 2016-03-02 Novozymes A/S Verfahren zur stabilen chromosomalen integration von mehreren kopien von genen
WO2002008409A2 (en) * 2000-07-21 2002-01-31 The United States Of America, As Represented By The Secretary Of Agriculture Methods for the replacement, translocation and stacking of dna in eukaryotic genomes
WO2004018635A2 (en) 2002-08-21 2004-03-04 Kosan Biosciences, Inc. Myxococcus xanthus bacteriophage mx9 transformation and integration system
EP1405908A1 (de) * 2002-10-04 2004-04-07 ProBioGen AG Herstellung von Zelllinien zur heterologen Expression
US20040209370A1 (en) * 2002-12-19 2004-10-21 Wonchul Suh Method for chromosomal engineering
CN101061214B (zh) * 2004-10-22 2012-12-05 诺维信公司 多个多核苷酸拷贝的稳定基因组整合
EP2527448A1 (de) * 2011-05-23 2012-11-28 Novozymes A/S Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze

Also Published As

Publication number Publication date
US11174487B2 (en) 2021-11-16
US20140273237A1 (en) 2014-09-18
WO2006042548A1 (en) 2006-04-27
EP1805296A1 (de) 2007-07-11
CN101061214B (zh) 2012-12-05
DK1805296T3 (da) 2010-01-18
ATE443128T1 (de) 2009-10-15
US20170218381A1 (en) 2017-08-03
US20080085535A1 (en) 2008-04-10
EP1805296B1 (de) 2009-09-16
CN101061214A (zh) 2007-10-24

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