AU2001265835A1 - Method for stable chromosomal multi-copy integration of genes - Google Patents

Method for stable chromosomal multi-copy integration of genes

Info

Publication number
AU2001265835A1
AU2001265835A1 AU2001265835A AU6583501A AU2001265835A1 AU 2001265835 A1 AU2001265835 A1 AU 2001265835A1 AU 2001265835 A AU2001265835 A AU 2001265835A AU 6583501 A AU6583501 A AU 6583501A AU 2001265835 A1 AU2001265835 A1 AU 2001265835A1
Authority
AU
Australia
Prior art keywords
genes
copy integration
stable chromosomal
chromosomal multi
stable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001265835A
Inventor
Jens Toenne Andersen
Steen Troels Jorgensen
Carsten Olsen
Michael Dolbjerg Rasmussen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of AU2001265835A1 publication Critical patent/AU2001265835A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
AU2001265835A 2000-06-23 2001-06-21 Method for stable chromosomal multi-copy integration of genes Abandoned AU2001265835A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200000981 2000-06-23
DKPA200000981 2000-06-23
US21792900P 2000-07-13 2000-07-13
US60217929 2000-07-13
PCT/DK2001/000436 WO2002000907A1 (en) 2000-06-23 2001-06-21 Method for stable chromosomal multi-copy integration of genes

Publications (1)

Publication Number Publication Date
AU2001265835A1 true AU2001265835A1 (en) 2002-01-08

Family

ID=26068840

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001265835A Abandoned AU2001265835A1 (en) 2000-06-23 2001-06-21 Method for stable chromosomal multi-copy integration of genes

Country Status (5)

Country Link
US (1) US6808896B2 (en)
EP (1) EP1297170B1 (en)
JP (1) JP2004501651A (en)
AU (1) AU2001265835A1 (en)
WO (1) WO2002000907A1 (en)

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US7227057B2 (en) 1997-06-03 2007-06-05 Chromatin, Inc. Plant centromere compositions
US7235716B2 (en) 1997-06-03 2007-06-26 Chromatin, Inc. Plant centromere compositions
US7989202B1 (en) 1999-03-18 2011-08-02 The University Of Chicago Plant centromere compositions
EP1290207B1 (en) * 2000-05-24 2010-09-08 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell
EP2213745A1 (en) 2002-04-10 2010-08-04 Novozymes A/S Improved bacillus host cell
AU2003214035A1 (en) * 2002-04-10 2003-10-27 Novozymes A/S Bacillus licheniformis mutant host cell
DK1497429T3 (en) * 2002-04-10 2008-11-03 Novozymes As Enhanced bacillus host cell
AU2003215527A1 (en) * 2002-04-10 2003-10-27 Novozymes A/S Bacillus licheniformis mutant host cell
AU2003223928A1 (en) * 2002-05-07 2003-11-11 Novozymes A/S Homologous recombination into bacterium for the generation of polynucleotide libraries
CN1902318A (en) 2003-10-31 2007-01-24 诺维信公司 Method for stable gene-amplification in a bacterial host cell
CN1969045A (en) 2003-12-10 2007-05-23 诺维信公司 Cell with improved secretion mediated by MrgA protein or homologue
WO2005083096A1 (en) * 2004-02-23 2005-09-09 Chromatin, Inc. Plants modified with mini-chromosomes
CN101006174B (en) 2004-06-21 2010-05-26 诺维信公司 Stably maintained multiple copies of at least two ORF in the same orientation
US20090104165A1 (en) * 2004-09-22 2009-04-23 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
US7553684B2 (en) * 2004-09-27 2009-06-30 Idc, Llc Method of fabricating interferometric devices using lift-off processing techniques
WO2006042548A1 (en) 2004-10-22 2006-04-27 Novozymes A/S Stable genomic integration of multiple polynucleotide copies
US8222028B2 (en) 2005-09-08 2012-07-17 Chromatin, Inc. Plants modified with mini-chromosomes
CN101541968B (en) 2006-11-30 2013-11-13 诺维信公司 Dnase expression recombinant host cells
US8614089B2 (en) * 2007-03-15 2013-12-24 Chromatin, Inc. Centromere sequences and minichromosomes
CN101981193B (en) * 2008-03-28 2016-05-11 丹尼斯科美国公司 The method of amplifying locus in bacterial cell
KR101072708B1 (en) * 2008-12-17 2011-10-11 씨제이제일제당 (주) A corynebacteria having enhanced 5'-xanthosine monophosphate productivity and a method of producing 5'-xanthosine monophosphate using the same
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
WO2011011693A1 (en) 2009-07-23 2011-01-27 Chromatin, Inc. Sorghum centromere sequences and minichromosomes
CN102712512A (en) 2009-11-10 2012-10-03 诺维信生物股份有限公司 Methods, compositions and systems for controlling fouling of a membrane
US20120329090A1 (en) 2009-12-21 2012-12-27 Novozymes A/S Methods for Producing Heterologous Polypeptides in Thiol-Disulfide Oxidoreductase-Deficient Bacterial Mutant Cells
CN102127530B (en) * 2010-11-29 2012-07-25 南宁邦尔克生物技术有限责任公司 Method for integrating two or more than two exogenous gene expression units onto the same integrated site of chromosome of Bacillus subtilis
CN102363763A (en) * 2011-10-21 2012-02-29 天津大学 Gene engineering bacteria for efficiently expressing human platelet factor 4 and construction method thereof
WO2014018602A1 (en) * 2012-07-26 2014-01-30 Glycos Biotechnologies, Inc. Strains and methods for plasmid maintenance
DK2898076T3 (en) * 2012-09-19 2018-06-14 Dsm Ip Assets Bv METHOD OF CELL MODIFICATION USING ESSENTIAL GENES AS MARKERS AND EVEN RECYCLING THESE
EP3481959A1 (en) 2016-07-06 2019-05-15 Novozymes A/S Improving a microorganism by crispr-inhibition
US11667980B2 (en) 2017-08-07 2023-06-06 Novozymes A/S Use of FCA control based on PH
AR112778A1 (en) 2017-08-07 2019-12-11 Novozymes As FERMENTER EQUIPPED WITH EJECTOR
US11879127B2 (en) 2017-08-23 2024-01-23 Danisco Us Inc. Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
CN108913706B (en) * 2018-07-10 2021-04-09 郑州轻工业学院 Bacillus subtilis glycerol kinase mutant gene glpK and application thereof
US20220298517A1 (en) 2019-06-25 2022-09-22 Novozymes A/S Counter-selection by inhibition of conditionally essential genes
US20230227834A1 (en) 2020-06-17 2023-07-20 Basf Se Plasmid Copy Number Regulation and Integration
EP4185689A1 (en) 2020-07-24 2023-05-31 Basf Se Alanine racemase single deletion and transcomplementation
BR112023027016A2 (en) 2021-06-24 2024-03-12 Basf Se MODIFIED BACILLUS HOST CELL COMPRISING AN ALTERED EXTRACELLULAR MATRIX REGULATORY PROTEIN A AND/OR AN ALTERED EXTRACELLULAR MATRIX REGULATORY PROTEIN B, METHOD FOR PRODUCING A COMPOUND OF INTEREST, AND, ALTERED EXTRACELLULAR MATRIX REGULATORY PROTEINS A AND B

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
AU620326B2 (en) * 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
DK639689D0 (en) 1989-12-18 1989-12-18 Novo Nordisk As INTRODUCING DNA IN CELLS
KR100237148B1 (en) 1990-05-09 2000-01-15 한센 핀 베네드 A cellulase preparation comprising an endoglucanase enzyme
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JPH10512450A (en) * 1995-01-23 1998-12-02 ノボ ノルディスク アクティーゼルスカブ DNA integration by transfer
AU7908998A (en) * 1997-07-03 1999-01-25 Novo Nordisk A/S Protein producing cell containing multiple copies of a desired gene and a screenable marker but no selection marker
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US6100063A (en) 1998-02-12 2000-08-08 Novo Nordisk A/S Procaryotic cell comprising at least two copies of a gene
AU2610399A (en) * 1998-02-12 1999-08-30 Novo Nordisk A/S A prokaryotic cell comprising two copies of a gene transcribed in different directions
EP0972838B1 (en) * 1998-07-15 2004-09-15 Roche Diagnostics GmbH Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy
EP1290207B1 (en) * 2000-05-24 2010-09-08 Novozymes A/S Method for increasing gene copy number in a host cell and resulting host cell

Also Published As

Publication number Publication date
US6808896B2 (en) 2004-10-26
EP1297170A1 (en) 2003-04-02
US20030032186A1 (en) 2003-02-13
JP2004501651A (en) 2004-01-22
WO2002000907A1 (en) 2002-01-03
EP1297170B1 (en) 2016-03-02

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