KR920703803A - 활성화 피브린 용해성과 항-혈전성 단백질 - Google Patents

활성화 피브린 용해성과 항-혈전성 단백질

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KR920703803A
KR920703803A KR1019920701348A KR920701348A KR920703803A KR 920703803 A KR920703803 A KR 920703803A KR 1019920701348 A KR1019920701348 A KR 1019920701348A KR 920701348 A KR920701348 A KR 920701348A KR 920703803 A KR920703803 A KR 920703803A
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compound
compound according
nucleic acid
protein
coagulation
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마틴 다우슨 케이스
마크 에드워즈 리차드
마벨 포먼 조안
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폴 리틀우드
브리티쉬 바이오-테크놀로지 리미티드
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Abstract

내용 없음

Description

활성화 피브린 용해성과 항-혈전성 단백질
[도면의 간단한 설명]
제1도는 pGWH의 구조도.
제3도는 표현 벡터 pGWHgP1의 지도.
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (25)

  1. 피브린 용해 활성을 갖기 위하여 또는 응혈 형성을 억제하기 위하여, 응혈에 포함되어 있는 효소에 의하여 활성화할 수 있는 단백질 화합물에 있어서, 이 화합물이 플라스민 활성을 갖도록 활성화할 수 있는 플라스미노겐 유사체임을 특징으로 하는 상기 화합물.
  2. 제1항에 있어서, 응혈에 포함되어 있는 효소가 칼리크레인, 인자 XIIa, XIa, IXa, VIIa, Xa, 트롬빈(인자 IIa) 또는 활성화 단백질 C임을 특징으로 하는 화합물.
  3. 제1항 또는 제2항에 있어서, 응혈에 포함되어 있는 효소가 인자 Xa 또는 트롬빈임을 특징으로 하는 화합물.
  4. 제1항 또는 제2항에 있어서, 응혈에 포함되어 있는 효소가 인자 Xa임을 특징으로 하는 화합물.
  5. 제4항에 있어서, P4가 소수성 잔재를 나타내고 P3가 산성 잔재를 나타내는 전단 부위 배열 P4-P3-Gly-Arg을 함유함을 특징으로 하는 화합물.
  6. 제5항에 있어서, 소수성 잔재가 이소류신임을 특징으로 하는 화합물.
  7. 제1항 또는 제2항에 있어서, 응혈에 포함되어 있는 효소가 트롬빈임을 특징으로 하는 화합물.
  8. 제7항에 있어서, P4와 P3가 각각 독립적으로 소수성 잔재를 나타내고 P1'와 P2'가 각각 독립적으로 비-산성 잔재를 나타내는 절단부위 배열 Pro-P3-Pro-Arg-P1′-P2′를 함유함을 특징으로 하는 화합물.
  9. 제7항에 있어서, 잔재 P2와 P1′ 중 하나가 글리신을 나타내고, 다른 하나가 아미노산 잔재를 나타냄을 특징으로 하는 화합물.
  10. 제7항에 있어서, 절단 부위 배열 Gly-Pro-Arg를 함유함을 특징으로 하는 화합물.
  11. 제1항에 있어서, 포함된 잔재 Pro(55)와 Cys(566)사이에 하나 또는 그 이상의 아미노산 치환물, 첨가물 또는 결실물을 가짐을 특징으로 하는 화합물.
  12. 제1항 또는 제11항에 있어서, 하나 또는 그 이상의 첨가물, 결실물 또는 치환물일 수 있는 하나 또는 그 이상 다른 변경물(야생형 qlu-플라스미노겐과 비교하여)을 함유함을 특징으로 하는 화합물.
  13. 연속 아미노산 잔재를 함께 결합시키고 올리고-과 또는 폴리-펩티드를 결합시킴을 특징으로 하는 제1항 내지 제12항 중 어느 한 항의 단백질 화합물의 제조방법.
  14. 제1항 내지 제12항 중 어느 한 항의 단백질 화합물을 암호화하는 합성 또는 재조합형 핵산.
  15. 제14항에 있어서, 벡터임을 특징으로 하는 핵산.
  16. 연속 누클레오티드를 함께 결합시키고 올리고-과/또는 폴리-누클레오티드를 결합시킴을 특징으로 하는 제14항의 핵산의 제조방법.
  17. 인체 시토메갈로바이러스에서 유도된 강한 프로모터와 인헨서 배열을 함유하는 제2핵산 배열에 조작적으로 연결되는 단백질을 암호화하거나 클론 부위를 규현하는 제1핵산 배열, SV40에서 유도된 폴리아데닐화 배열을 기호화하는 제3핵산 배열과 SV40 프로모터로부터 표현된 qpt선택성 표지를 암호화하고 선택성 표지 배열의 3'말단에서 부가적 SV40 폴리아데닐화 신호를 갖는 제4핵산 배열로 이루어짐을 특징으로 하는 벡터.
  18. 제17항에 있어서, 제1핵산 배열이 플라스미노겐 또는 플라스미노겐 유사체를 암호화함을 특징으로 하는 벡터.
  19. 제15항, 제17항 또는 제18항에서 특허청구된 벡터로 형질전환되거나, 트란스펙트된 세포 또는 세포선.
  20. 제19항에 있어서, 연속 배양으로 성장하는 포유류 세포로 이루어지는 세포선.
  21. 플라스미노겐 또는 플라스미노겐 유사체를 표현하도록 형질 전환된 중국 햄스터 난소 세포.
  22. 제1항 내지 제12항 중 어느 한 항의 하나 또는 그 이상 화합물과 약학적 또는 수의학적으로 허용할 수 있는 담체로 이루어짐을 특징으로 하는 제약적 조성물.
  23. 비독성, 유효량의 제1항 내지 제12항중 어느 한 항의 화합물을 투여하여서 함을 특징으로 하는 혈전성 질별의 치료 또는 예방방법.
  24. 의약품 또는 수의약품으로 사용하는 제1항 내지 제12항중 어느 한 항의 단백질 화합물.
  25. 혈전 분해제 또는 항혈전제를 제조하는데 제1항 내지 제12항중 어느 한 항의 화합물을 상요하는 화합물의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019920701348A 1989-12-07 1990-12-07 활성화피브린용해성과항-혈전성단백질 KR100188302B1 (ko)

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GB898927722A GB8927722D0 (en) 1989-12-07 1989-12-07 Proteins and nucleic acids
PCT/GB1990/001912 WO1991009118A2 (en) 1989-12-07 1990-12-07 Activatable fibrinolytic and anti-thrombotic proteins

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PT96103A (pt) 1991-09-30
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DE69031127D1 (de) 1997-09-04
WO1991009118A2 (en) 1991-06-27
FI922609A0 (fi) 1992-06-05
NZ236330A (en) 1993-05-26
AU644399B2 (en) 1993-12-09
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ZA909854B (en) 1992-08-26
AU4497693A (en) 1993-11-18
NO305562B1 (no) 1999-06-21
GB8927722D0 (en) 1990-02-07
KR920703818A (ko) 1992-12-18
NO922238L (no) 1992-08-06
AU6965691A (en) 1991-07-18
WO1991009125A1 (en) 1991-06-27
DK0502968T3 (da) 1998-03-02
AU643247B2 (en) 1993-11-11
US5637492A (en) 1997-06-10
DE69031127T2 (de) 1998-02-05
FI922609A (fi) 1992-06-05
IE81046B1 (en) 1999-12-01
CA2069105A1 (en) 1991-06-08
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US5434073A (en) 1995-07-18
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ATE155812T1 (de) 1997-08-15
PT96104A (pt) 1991-09-30
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ES2106073T3 (es) 1997-11-01
GR3024990T3 (en) 1998-01-30
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PT96103B (pt) 1998-08-31
IL96602A0 (en) 1992-05-25
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CA2069085A1 (en) 1991-06-08
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