KR910020172A - 항 종양성 덱스트란의 제조법 - Google Patents

항 종양성 덱스트란의 제조법 Download PDF

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KR910020172A
KR910020172A KR1019910008633A KR910008633A KR910020172A KR 910020172 A KR910020172 A KR 910020172A KR 1019910008633 A KR1019910008633 A KR 1019910008633A KR 910008633 A KR910008633 A KR 910008633A KR 910020172 A KR910020172 A KR 910020172A
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ferm
strain
reaction
dextran
glucan
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히사오 가또
야스가즈 나가기따
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이라까와 도시오
삿뽀로 비루 가부시끼가이샤
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Publication of KR910020172A publication Critical patent/KR910020172A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08—Dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
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    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/823—Acetobacter

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Abstract

내용 없음

Description

항 종양성 덱스트란의 제조법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명에서 얻어진 항 종양성 덱스트란의 UV (자외선)흡수 스펙트럼, 제2도는 같은 물질의 IR (적외선)흡수 스펙트럼, 제3도는 같은 물질의 13C―NMR 스펙트럼.

Claims (5)

  1. 락트산균 콘퓨써스(Lactobacillus Confusus)에 속하고, 하기의 성질을 갖는 덱스트란을 생산하는 능력을 갖는 미생물을 배양하고, 배양물로부터 그 물질을 채취하는 것을 특징으로 하는 항 종양성 덱스트란의 제조법.
    (1). 성상 : 무미, 무취의 백색 무정형 분말 (2). 용해성 : 물, 포롬 아미드, 디메틸 술폭시드에 가용, 알코올, 아세톤, 헥산, 클로로포름, 사염화 탄소에 불용 (3). 수용액의 PH : 중성 또는 약산성 (4). 구성당 : 포도당만으로 구성 (5). 원소 분석값 : C : 43―45%, H : 6.0―6.3% (6). 구조 : 선형 α―1.6 글루코시드 결합을 주 결합으로 하는 α―글루칸 (7). 단백질 : 로우 리법으로는 검출되지 않음. (8). 분자량 : 투석막을 통과하지 않고, 분자량이 10,000이상으로 추정된다. (9). 발색반응 : 안트론 황산 반응, 페놀 황산 반응은 양성, 뷰렛반응, 로우리―포오린(Lowery―folin)반응, 엘슨―모르간(Elson―Morgan)반응, 요오드 반응은 음성. (10). 융점 : 융점이 명확하지 않다. (11). 자외선 흡수 스펙트럼 : 특징적 흡수가 없다. (12). 적외선 흡수 스펙트럼 : α―글루칸이 특징적 흡수를 나타낸다. (13).13C―NMR 스펙트럼 : α―1.6―글루칸이 특징적 스펙트럼을 나타낸다. (14). 항 종양 작용을 갖는다.
  2. 제1항에 있어서, 항 종양성 덱스트란 생산 능력을 갖는 미생물이 락트산균 콘퓨써스(Lactobacillus confusus) 40―1주 (FERM BP―2865), 40―3주 (FERM BP―2866), 77―1주 (FERM BP―2867), 78―1주 (FERM BP―2868), 80―1주 (FERM BP―2869) 및 이들의 변이주로 된 군으로부터 선택된 1종의 미생물인 항 종양성 덱스트란의 제조법.
  3. 락트산균 콘퓨써스에 속하고 하기의 성질을 갖는 덱스트란을 생산하는 능력을 갖는 미생물을 배양학 생산된 항 종양성 덱스트란 합성 효소를 회수하며 그 효소를 설탕에 작용시키는 것을 특징으로 하는 양 조양성 덱스트란의 제조법.
    (1). 성상 : 무미, 무취의 백색 무정형 분말 (2). 용해성 : 물, 포롬 아미드, 디메틸 술폭시드에 가용, 알코올, 아세톤, 헥산, 클로로포름, 사염화 탄소에 불용 (3). 수용액의 PH : 중성 또는 약산성 (4). 구성당 : 포도당만으로 구성 (5). 원소 분석값 : C : 43―45%, H : 6.0―6.3% (6). 구조 : 선형 α―1.6 글루코시드 결합을 주 결합으로 하는 α―글루칸 (7). 단백질 : 로우 리법으로는 검출되지 않음. (8) 분자량 : 투석막을 통과하지 않고, 분자량이 10,000이상으로 추정된다. (9). 발색반응 : 안트론 황산 반응, 페놀 황산 반응은 양성, 뷰렛반응, 로우리―포오린 반응, 엘슨―모르간 반응, 요오드 반응은 음성. (10) 융점 : 융점이 명확하지 않다. (11). 자외선 흡수 스펙트럼 : 특징적 흡수가 없다. (12). 적외선 흡수 스펙트럼 : α―글루칸이 특징적 흡수를 나타낸다. (13). 13C―NMR 스펙트럼 : α―1.6―글루칸이 특징적 스펙트럼을 나타낸다. (14). 항 종양 작용을 갖는다.
  4. 제3항에 있어서, 항 종양성 덱스트란 생산 능력을 갖는 미생물이 락트산균 콘퓨써스 40―1주 (FERM BP―2865), 40―3주 (FERM BP―2866), 77―1주 (FERM BP―2867), 78―1주 (FERM BP―2868), 80―1주 (FERM BP―2869) 및 이들의 변이주로 된 군으로부터 선택된 1종의 미생물인 항 종양성 덱스트란의 제조법.
  5. 락트산균 콘퓨써스 40―1주 (FERM BP―2865), 40―3주 (FERM BP―2866), 77―1주 (FERM BP―2867), 78―1주 (FERM BP―2868), 80―1주 (FERM BP―2869).
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019910008633A 1990-05-31 1991-05-27 항 종양성 덱스트란의 제조법 KR910020172A (ko)

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JP90-139906 1990-05-31
JP2139906A JPH0436187A (ja) 1990-05-31 1990-05-31 抗腫瘍性デキストランの製造法

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US (2) US5424201A (ko)
EP (1) EP0459367B1 (ko)
JP (1) JPH0436187A (ko)
KR (1) KR910020172A (ko)
CA (1) CA2042536A1 (ko)
DE (1) DE69116601T2 (ko)
ES (1) ES2085377T3 (ko)

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US5744111A (en) * 1992-07-03 1998-04-28 Kabushiki Kaisha Riken Method for cleaning exhaust gas
CA2160082C (en) * 1994-10-07 1998-11-10 Melvin E. Klegerman Mixture having antitumor activities
JP4042813B2 (ja) * 1997-10-09 2008-02-06 名糖産業株式会社 ホウ素の含有量が低減されたデキストランの製造方法
US5869645A (en) * 1997-10-15 1999-02-09 Board Of Trustees Of The University Of Illinois Method for isolating high molecular weight antineoplastic glycans using urea
US6975892B2 (en) * 2003-10-21 2005-12-13 Oculir, Inc. Methods for non-invasive analyte measurement from the conjunctiva
US6958039B2 (en) * 2003-05-02 2005-10-25 Oculir, Inc. Method and instruments for non-invasive analyte measurement
US6968222B2 (en) 2003-05-02 2005-11-22 Oculir, Inc. Methods and device for non-invasive analyte measurement
US20060224057A1 (en) * 2003-10-21 2006-10-05 Oculir, Inc. Methods for non-invasive analyte measurement
US20080009688A1 (en) * 2004-04-14 2008-01-10 Oculir, Inc. Methods for non-invasive analyte measurement
US20060258919A1 (en) * 2004-04-14 2006-11-16 Oculir, Inc. Non-Invasive Analyte Measurement Device for Measuring Tears and Other Ocular Elements Using Electromagnetic Radiation and Method of Using the Same
WO2008023664A1 (fr) * 2006-08-21 2008-02-28 Sapporo Breweries Limited Souche bactérienne possédant une activité anti-allergique, et boisson, aliment et agent anti-allergique comprenant la souche bactérienne
CN110178897A (zh) * 2019-05-27 2019-08-30 安徽工程大学 一种低血糖生成指数的谷物发酵乳及其制备方法

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US4396763A (en) * 1981-01-14 1983-08-02 Meiji Milk Products Company Limited High molecular polysaccharide MPS-80
JPS601877B2 (ja) * 1981-01-14 1985-01-17 明治乳業株式会社 高分子多糖類物質ｍｐｓ−８０及びその製造法
DE3374695D1 (en) * 1982-09-14 1988-01-07 Degussa L-2-hydroxy-4-methylpentanoic acid dehydrogenase, process for its production and its use
US4814273A (en) * 1985-03-20 1989-03-21 Michigan Biotechnology Institute Production of organic acids by an improved fermentation process
JPH01277484A (ja) * 1988-04-30 1989-11-07 Kanebo Ltd 抗腫瘍活性を有する乳酸菌株およびその培養物
JPH01281073A (ja) * 1988-05-06 1989-11-13 Kanebo Ltd 抗腫瘍活性を有する乳酸菌株およびその培養物
JP2555417B2 (ja) * 1988-06-24 1996-11-20 雪印乳業株式会社 乳酸菌培養物を有効成分とする抗腫瘍剤
JPH03291232A (ja) * 1990-04-09 1991-12-20 Sumitomo Heavy Ind Ltd 抗腫瘍、免疫賦活剤

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CA2042536A1 (en) 1991-12-01
DE69116601T2 (de) 1996-07-18
DE69116601D1 (de) 1996-03-07
US5424201A (en) 1995-06-13
ES2085377T3 (es) 1996-06-01
US5484715A (en) 1996-01-16
EP0459367B1 (en) 1996-01-24
EP0459367A1 (en) 1991-12-04
JPH0436187A (ja) 1992-02-06

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