KR900013077A - 글루카곤의 제조방법 - Google Patents
글루카곤의 제조방법 Download PDFInfo
- Publication number
- KR900013077A KR900013077A KR1019900001157A KR900001157A KR900013077A KR 900013077 A KR900013077 A KR 900013077A KR 1019900001157 A KR1019900001157 A KR 1019900001157A KR 900001157 A KR900001157 A KR 900001157A KR 900013077 A KR900013077 A KR 900013077A
- Authority
- KR
- South Korea
- Prior art keywords
- glucagon
- fusion protein
- trp
- amino acid
- glu
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/57—IFN-gamma
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Endocrinology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Steroid Compounds (AREA)
- Control Of El Displays (AREA)
- Earth Drilling (AREA)
- Diaphragms And Bellows (AREA)
- Separation By Low-Temperature Treatments (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 인간 글루카곤을 암호화하는 DNA서열을 나타낸다.
제2(a)도는 본 발명의 발현 벡터 Trp pAT huFNr/글루카곤의 제조에 사요오디는 플라스미드 pGA1의 제조를 나타내는 개략도이다.
제3도는 DNA의 상류 또는 하류에 인접한 부위 뿐만 아니라 발현벡터 Trp pAT huFNr/글루카곤을 함유하는 글루카곤을 암호화하는 DNA염기 서열을 나타낸다.
Claims (11)
1. 다음식(Ⅰ)의 표시되는 융합단백질을 제조하고
x―Glu―Glucagon (Ⅰ)
(여기에서, X는 70내지170개의 아미노산 리더 서열이고, Glu는 글루탐산 잔기이고, Glucagon은 글루카곤의 아미노산 서열을 나타낸다), 상기 융합 단백질을 글루탐산 잔기의 카복실 말단에서 펩티드결합을 특이적으로 가수분해하는 효소로 처리하여 융합 단백질을 절단하는 것을 포함하는 글루카곤의 제조방법.
제1항에 있어서, 서열 X가 인터페론―r, △ Cys 인터페론―r 또는 인터페론 α80A2의 아미노 말단 서열일부를 함유하는 방법.
제1항에 있어서, 글루카곤의 아미노산 서열이 다음식(Ⅱ)으로 표시되는 방법.
His Ser Gln Gly Thr Phe Thr Ser Asp Yyr
Ser Lys Tyr Leu Asp Ser Arg Arg Ala Gln
Asp Phe Val Gln Trp Leu Met Asn Thr (Ⅱ)
제1항에 있어서, 융합 단백질을 식(Ⅰ)로 표시되는 융합 단백질을 발현하는 발현 벡터를E. coli로 도입함으로써 얻어지는 형질전환체를 배양시키는 방법으로 제조하는 방법.
제1항에 있어서, 글루카곤을 (a)융합단백질을 적절한 변성화제 또는 계면활성제로 용해시키고, (b)변성제 또는 계면활성제의 농도를 저하시키고, (c)(b)단계에서 얻은 혼합물에 얻은 혼합물을에 효소를 가하여 융합 단백질의 부분 분해에서 생성되는 가용성 중간물질 함유한 반응 혼합물을 얻고 (d)반응혼합물을 탈염시켜서 중간물질 및 효소를 함유하고 변성제 또는 계면활성제는 함유하지 않는 획분을 얻고, (e)획분에 함유된 효소에 의해 반응을 더 진행시켜서 글루코겐을 방출시키고, (f)방출된 글루코겐을 분리시키는 것을 포함하는 단계에 의해 회수하는 방법.
제1항에 있어서, 효소가스타필로코쿠스 아우레우스로부터 유도된 프로테아제 V8인 방법.
다음식 (Ⅲ)으로 표시되는, 글루카곤을 암호화하는 DNA서열.
5'―CACTCTCAGGGTACCTTCACTTCCGACTACTCTAAA
TACCTGGACTCCCGTCGTGCTCAGGACTTCGTTCAG
TGGCTGATGAACACC―3' (Ⅲ)
다음식(Ⅰ)으로 표시되는 융합 단백질을 암호화하는 DNA서열을 포함하는 발현벡터
X―Glu―Glucagon (Ⅰ)
X는 70내지 170개의 아미노산 리더 서열이고, Glu는 글루탐산 잔기이고, Glucagon은 글루카곤의 아미노산 서열을 나타낸다.
제8항에 있어서, 발현벡터가 Trp pAT huIFN r/글루카곤, Trp pAT△Cys huIFN r/글루카곤, Trp pAT△Cys쇼트 huIFN r/글루카곤, Trp pAT huIFN r80A2/글루카곤 및 Trp pAT△CysⅡhuIFN r/글루카곤으로 구성된 그룹에서 선택된 발현 벡터.
제8항 또는 제9항의 발현 벡터를E. coli의 세포로 도입하여 얻는 형절전환체.
고성능 액체 크로마토그래프로 측정하여 99% 이상의 순도를 갖는 정제된 글루카곤.
※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP89-24158 | 1989-02-01 | ||
JP1-24158 | 1989-02-01 | ||
JP2415889 | 1989-02-01 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR900013077A true KR900013077A (ko) | 1990-09-03 |
KR0161656B1 KR0161656B1 (ko) | 1998-11-16 |
Family
ID=12130531
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019900001157A KR0161656B1 (ko) | 1989-02-01 | 1990-02-01 | 글루카곤의 생산방법 |
Country Status (18)
Country | Link |
---|---|
EP (1) | EP0381433B1 (ko) |
JP (1) | JPH0748999B2 (ko) |
KR (1) | KR0161656B1 (ko) |
CN (1) | CN1063795C (ko) |
AT (1) | ATE144551T1 (ko) |
BR (1) | BR9000445A (ko) |
CA (1) | CA2008824C (ko) |
DE (1) | DE69028935T2 (ko) |
DK (1) | DK0381433T3 (ko) |
ES (1) | ES2095236T3 (ko) |
FI (1) | FI900497A0 (ko) |
GR (1) | GR3021965T3 (ko) |
HU (1) | HUT54410A (ko) |
IL (1) | IL93173A0 (ko) |
NO (1) | NO900410L (ko) |
NZ (1) | NZ232255A (ko) |
PT (1) | PT93031A (ko) |
ZA (1) | ZA90533B (ko) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6506595B2 (en) * | 1998-03-31 | 2003-01-14 | Itoham Foods Inc. | DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs |
CN100480374C (zh) * | 2001-09-28 | 2009-04-22 | 中国科学院上海生命科学研究院 | 制备重组人类胰高血糖素肽-1氨基酸7-37肽段的方法 |
BR112012024379A2 (pt) * | 2010-03-26 | 2017-01-10 | Novo Nordisk As | "peptídeos glucagon, seu uso, bem como composição farmacêutica" |
MX354705B (es) | 2011-09-23 | 2018-03-16 | Novo Nordisk As | Analogos de glucagon novedosos. |
MY174727A (en) | 2013-04-18 | 2020-05-11 | Novo Nordisk As | Stable, protracted glp-1/glucagon receptor co-agonists for medical use |
US10570184B2 (en) | 2014-06-04 | 2020-02-25 | Novo Nordisk A/S | GLP-1/glucagon receptor co-agonists for medical use |
CN104593399A (zh) * | 2014-12-22 | 2015-05-06 | 广西大学 | 一种转基因胰岛素的合成工艺 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK27885A (da) * | 1985-01-22 | 1986-07-23 | Novo Industri As | Fremstilling af peptider |
US4743679A (en) * | 1986-02-24 | 1988-05-10 | Creative Biomolecules, Inc. | Process for producing human epidermal growth factor and analogs thereof |
-
1990
- 1990-01-25 IL IL93173A patent/IL93173A0/xx unknown
- 1990-01-25 ZA ZA90533A patent/ZA90533B/xx unknown
- 1990-01-26 NZ NZ232255A patent/NZ232255A/en unknown
- 1990-01-29 CA CA002008824A patent/CA2008824C/en not_active Expired - Fee Related
- 1990-01-29 NO NO90900410A patent/NO900410L/no unknown
- 1990-01-30 AT AT90300937T patent/ATE144551T1/de not_active IP Right Cessation
- 1990-01-30 DK DK90300937.1T patent/DK0381433T3/da active
- 1990-01-30 EP EP90300937A patent/EP0381433B1/en not_active Expired - Lifetime
- 1990-01-30 ES ES90300937T patent/ES2095236T3/es not_active Expired - Lifetime
- 1990-01-30 DE DE69028935T patent/DE69028935T2/de not_active Expired - Fee Related
- 1990-01-31 FI FI900497A patent/FI900497A0/fi not_active IP Right Cessation
- 1990-01-31 HU HU90614A patent/HUT54410A/hu unknown
- 1990-02-01 BR BR909000445A patent/BR9000445A/pt unknown
- 1990-02-01 PT PT93031A patent/PT93031A/pt not_active Application Discontinuation
- 1990-02-01 KR KR1019900001157A patent/KR0161656B1/ko not_active IP Right Cessation
- 1990-02-01 CN CN90101167A patent/CN1063795C/zh not_active Expired - Fee Related
- 1990-02-01 JP JP2022825A patent/JPH0748999B2/ja not_active Expired - Fee Related
-
1996
- 1996-12-11 GR GR960403387T patent/GR3021965T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
DK0381433T3 (da) | 1996-11-18 |
KR0161656B1 (ko) | 1998-11-16 |
FI900497A0 (fi) | 1990-01-31 |
PT93031A (pt) | 1990-08-31 |
HU900614D0 (en) | 1990-04-28 |
NO900410D0 (no) | 1990-01-29 |
ZA90533B (en) | 1990-10-31 |
ES2095236T3 (es) | 1997-02-16 |
DE69028935D1 (de) | 1996-11-28 |
CA2008824C (en) | 1997-08-05 |
JPH0748999B2 (ja) | 1995-05-31 |
CN1046559A (zh) | 1990-10-31 |
EP0381433A1 (en) | 1990-08-08 |
DE69028935T2 (de) | 1997-03-06 |
NO900410L (no) | 1990-08-02 |
GR3021965T3 (en) | 1997-03-31 |
JPH03254692A (ja) | 1991-11-13 |
EP0381433B1 (en) | 1996-10-23 |
AU4898990A (en) | 1990-08-09 |
CN1063795C (zh) | 2001-03-28 |
NZ232255A (en) | 1992-03-26 |
IL93173A0 (en) | 1990-11-05 |
CA2008824A1 (en) | 1990-08-01 |
ATE144551T1 (de) | 1996-11-15 |
HUT54410A (en) | 1991-02-28 |
BR9000445A (pt) | 1991-01-15 |
AU634999B2 (en) | 1993-03-11 |
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