KR850003843A - 신규의 빌리루빈 산화효소 및 그것을 포함하는 시약조성물 및 그의 사용방법 - Google Patents
신규의 빌리루빈 산화효소 및 그것을 포함하는 시약조성물 및 그의 사용방법 Download PDFInfo
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- KR850003843A KR850003843A KR1019840005904A KR840005904A KR850003843A KR 850003843 A KR850003843 A KR 850003843A KR 1019840005904 A KR1019840005904 A KR 1019840005904A KR 840005904 A KR840005904 A KR 840005904A KR 850003843 A KR850003843 A KR 850003843A
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- KR
- South Korea
- Prior art keywords
- bilirubin
- oxidase
- novel
- reagent composition
- bilirubin oxidase
- Prior art date
Links
- 108010015428 Bilirubin oxidase Proteins 0.000 title claims description 13
- 238000000034 method Methods 0.000 title claims 8
- 239000000203 mixture Substances 0.000 title claims 8
- 239000003153 chemical reaction reagent Substances 0.000 title claims 6
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 claims description 36
- 238000000862 absorption spectrum Methods 0.000 claims description 3
- PHOLIFLKGONSGY-CSKARUKUSA-N (e)-(3-methyl-1,3-benzothiazol-2-ylidene)hydrazine Chemical compound C1=CC=C2S\C(=N\N)N(C)C2=C1 PHOLIFLKGONSGY-CSKARUKUSA-N 0.000 claims description 2
- 229940009976 deoxycholate Drugs 0.000 claims 3
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 claims 3
- 239000003112 inhibitor Substances 0.000 claims 3
- 239000012085 test solution Substances 0.000 claims 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims 2
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 claims 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 2
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 2
- 229910052802 copper Inorganic materials 0.000 claims 2
- 239000010949 copper Substances 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- RCNSAJSGRJSBKK-NSQVQWHSSA-N Biliverdin IX Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(\C=C/2C(=C(C)C(=C/C=3C(=C(C=C)C(=O)N=3)C)/N\2)CCC(O)=O)N1 RCNSAJSGRJSBKK-NSQVQWHSSA-N 0.000 claims 1
- 241000127897 Ganoderma tsunodae Species 0.000 claims 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims 1
- 239000002211 L-ascorbic acid Substances 0.000 claims 1
- 235000000069 L-ascorbic acid Nutrition 0.000 claims 1
- 108090000854 Oxidoreductases Proteins 0.000 claims 1
- 102000004316 Oxidoreductases Human genes 0.000 claims 1
- 102000003992 Peroxidases Human genes 0.000 claims 1
- 238000002835 absorbance Methods 0.000 claims 1
- 229960005070 ascorbic acid Drugs 0.000 claims 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
- 210000000349 chromosome Anatomy 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims 1
- 238000002523 gelfiltration Methods 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 239000011259 mixed solution Substances 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 108040007629 peroxidase activity proteins Proteins 0.000 claims 1
- NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 claims 1
- 238000011002 quantification Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000001055 blue pigment Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000001054 red pigment Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
- C12Y103/03005—Bilirubin oxidase (1.3.3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명에 의해 얻어진 신규 빌리루빈 산화효소 M-1의 pH와 활성의 관계를 나타내고;
제2도는 온도와 활성의 관계를 나타내고;
제5도는 신규 빌리루빈 산화효소 M-1으로 얻어진 MBTH와 빌리루빈의 반응생성물(적색색소)의 흡수스펙트럼(점선)을 설명하는 그래프와, 염산산성 조건하에 생성된 청색색소의 흡수스펙트럼(실선)을 설명하는 그래프.
Claims (11)
- 다음과 같은 이화학적 성질을 갖는 신규의 빌리루빈 산화효소 M-1. (1) 작용:산소존재하에서 빌리루빈을 산화시켜 빌리베르딘, 담자색물질을 거쳐 거의 무색의 물질을 생성하는 작용을 갖지만, 과산화수소는 생성하지 않는다. (2) 기질특이성:빌리루빈에 작용하고, 빌리베르딘에도 어느정도 작용하며, 페놀, 카테콜 및 하이드로퀴논에도 작용. (3) 최적 pH 및 pH안정성:pH 5.5부근에서 최적 pH를 가지며, 37℃에서 60분간 처리에 pH 5와 pH 9사이에서 안정. (4) 최적온도 및 열안정성:50℃부근에서 최적온도를 가지며, pH 7.0에서 10분간 처리에 55℃까지 안정. (5) 분자량:약 83,000(겔여과법). (6) 등전점:3.92±0.05. (7) 저해제:아지드나트륨, L-아스코르빈산, 디티오쓰레이톨 및 시안화칼륨에 의해 저해됨. (8) 가시부 흡수스펙트럼:610nm부근에서 최대흡광도를 갖는다. (9) 당함량:약 4.5%의 당을 함유. (10) 구리함량:1몰당 구리 4몰을 함유.
- 신규 빌리루빈 산화효소 M-1을 함유하는 것을 특징으로 하는 빌리루빈용 시약조성물.
- 제2항에 있어서, 데옥시콜산염을 함유하는 빌리루빈용 시약조성물.
- 빌리루빈함유 피검액에 신규 빌리루빈 산화효소 M-1을 함유하는 빌리루빈용 시약조성물을 작용시키고, 그로 인한 빌리루빈의 변화를 비색학적으로 측정하는 것을 특징으로 하는 빌리루빈의 정량방법.
- 제4항에 있어서, 상기한 조성물이 데옥시콜산염을 함유하는 방법.
- 빌리루빈함유 피검액에 3-메틸-2-벤조티아졸리논-히드라존과 신규 빌리루빈 산화효소 M-1을 함유하는 빌리루빈용 시약조성물을 첨가하여 이 혼합용액을 반응시키고 반응액을 산성화한 후, 비색법으로 빌리루빈을 정량하는 것을 특징으로 하는 빌리루빈의 정량방법.
- 산화효소, 과산화효소 및 수소공급 색원체의 반응을 이용하여 빌리루빈이 저해제로 작용하는 빌리루빈함유 피검액중의 빌리루빈 이외의 성분을 분석하는 방법에 있어서, 상기한 방법이 (a) 신규 빌리루빈 산화효소 M-1을 함유하는 빌리루빈용 시약조성물을 피검액에 작용시켜 공존하는 빌리루빈의 저해효과를 제거하는 단계 및 (b) 잔존 피검액중의 목적성분을 분석하는 단계를 포함하는 것을 특징으로 하는 분석방법.
- 제7항에 있어서, (a)단계가 완결된 후에 신규 빌리루빈 산화효소 M-1저해물질을 첨가하고, 그 후 (b)단계를 수행하는 분석방법.
- 제7항 또는 제8항에 있어서, 상기한 조성물이 데옥시콜산염을 함유하는 분석방법.
- 트라키데르마속에 속하는 신규 빌리루빈 산화효소 M-1 생산균주를 배양하고, 생산된 신규 빌리루빈 산화효소 M-1을 배양액으로부터 분리시키는 것을 특징으로 하는 신규 빌리루빈 산화효소 M-1의 제조방법.
- 제10항에 있어서, 트라키데르마속에 속하는 상기한 신규 빌리루빈 산화효소 M-1 생산균주가 트라키데르마 쓰노데(Trachyderma tsunodae) k-2593인 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP218895 | 1983-11-21 | ||
| JP58218895A JPS60110290A (ja) | 1983-11-21 | 1983-11-21 | 新規ビリルビン・オキシダ−ゼおよびその製造法 |
| JP10560484A JPS60249060A (ja) | 1984-05-24 | 1984-05-24 | ビリルビン用試薬組成物及びその使用方法 |
| JP105604 | 1984-05-24 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR850003843A true KR850003843A (ko) | 1985-06-29 |
| KR880000753B1 KR880000753B1 (ko) | 1988-05-04 |
Family
ID=26445857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019840005904A KR880000753B1 (ko) | 1983-11-21 | 1984-09-26 | 신규의 빌리루빈 산화효소를 포함하는 시약조성물 및 그의 사용방법 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4600689A (ko) |
| KR (1) | KR880000753B1 (ko) |
| CA (1) | CA1219792A (ko) |
| DE (1) | DE3436005C2 (ko) |
| FR (1) | FR2555196B1 (ko) |
| GB (1) | GB2146997B (ko) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3249743C2 (ko) * | 1982-02-18 | 1990-10-04 | Amano Pharmaceutical Co., Ltd., Nagoya, Aichi, Jp | |
| US4701411A (en) * | 1983-09-01 | 1987-10-20 | Eastman Kodak Company | Bilirubin-specific enzyme preparation, assay compositions, analytical elements and methods using same |
| DE3608453A1 (de) * | 1986-03-14 | 1987-09-17 | Boehringer Mannheim Gmbh | Verfahren zur enzymatischen bestimmung von bilirubin im serum |
| US4746606A (en) * | 1986-05-27 | 1988-05-24 | Eastman Kodak Company | Bilirubin-specific enzyme and its analytical use |
| JP2578430B2 (ja) * | 1987-06-10 | 1997-02-05 | 旭化成工業株式会社 | 新規なビリルビン．オキシダーゼおよびその製造法 |
| US4937186A (en) * | 1988-01-28 | 1990-06-26 | Iqbal Siddiqi | Method for the determination of bilirubin in solution |
| JP2856757B2 (ja) * | 1989-03-13 | 1999-02-10 | ユニチカ株式会社 | 総ビリルビンの測定方法および測定用試薬 |
| JPH0771515B2 (ja) * | 1989-12-18 | 1995-08-02 | 日本商事株式会社 | ビリルビンの光学的測定法および測定用試薬 |
| US5229296A (en) * | 1991-12-30 | 1993-07-20 | Miles Inc. | Use of polymer-copper complex to control interference of biological substances in colorimetric test systems |
| JP3091094B2 (ja) * | 1993-12-28 | 2000-09-25 | ユニチカ株式会社 | 直接型ビリルビン測定用試薬 |
| KR970022316A (ko) * | 1995-10-27 | 1997-05-28 | 후루야 아끼라 | 빌리루빈의 정량 방법 |
| US5783407A (en) * | 1996-04-05 | 1998-07-21 | Beckman Instruments, Inc. | Method of measuring bilirubin |
| JP3734115B2 (ja) * | 1997-02-28 | 2006-01-11 | 日東紡績株式会社 | ビリルビン画分の測定方法 |
| US6030802A (en) * | 1998-05-29 | 2000-02-29 | Roche Diagnostics Corporation | Liquid reagent set for L-lactate determination |
| FR2957934B1 (fr) | 2010-03-24 | 2014-10-17 | Centre Nat Rech Scient | Bilirubine oxydase de bacillus pumilus et ses applications |
| FR2975704B1 (fr) | 2011-05-24 | 2015-02-20 | Centre Nat Rech Scient | Bilirubine oxydase de magnaporthe oryzae et ses applications |
| CN108918435A (zh) * | 2018-04-17 | 2018-11-30 | 武汉景川诊断技术股份有限公司 | 胆红素测定方法及试剂盒 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4211844A (en) * | 1978-05-19 | 1980-07-08 | Eastman Kodak Company | Bilirubin-specific fungal enzyme preparation |
| US4221844A (en) * | 1978-09-01 | 1980-09-09 | Hasenour James A | Decorative coating of metal |
| JPS6012031B2 (ja) * | 1981-03-30 | 1985-03-29 | 天野製薬株式会社 | ビリルビンオキシダ−ゼの製造法 |
| DE3249743C2 (ko) * | 1982-02-18 | 1990-10-04 | Amano Pharmaceutical Co., Ltd., Nagoya, Aichi, Jp | |
| JPS59125899A (ja) * | 1982-12-29 | 1984-07-20 | Nippon Shoji Kk | 酵素法による直接ビリルビン測定用試薬および測定法 |
-
1984
- 1984-09-10 US US06/649,054 patent/US4600689A/en not_active Expired - Fee Related
- 1984-09-11 GB GB08422935A patent/GB2146997B/en not_active Expired
- 1984-09-24 CA CA000463878A patent/CA1219792A/en not_active Expired
- 1984-09-26 KR KR1019840005904A patent/KR880000753B1/ko not_active IP Right Cessation
- 1984-10-01 DE DE3436005A patent/DE3436005C2/de not_active Expired
- 1984-10-19 FR FR8416064A patent/FR2555196B1/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| GB2146997A (en) | 1985-05-01 |
| FR2555196B1 (fr) | 1987-05-15 |
| KR880000753B1 (ko) | 1988-05-04 |
| DE3436005A1 (de) | 1985-05-30 |
| CA1219792A (en) | 1987-03-31 |
| GB2146997B (en) | 1987-06-24 |
| GB8422935D0 (en) | 1984-10-17 |
| FR2555196A1 (fr) | 1985-05-24 |
| DE3436005C2 (de) | 1986-11-06 |
| US4600689A (en) | 1986-07-15 |
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