KR20220051401A - 배양 중 개선된 지속성을 갖는 유전적으로 조작된 t 세포 - Google Patents

배양 중 개선된 지속성을 갖는 유전적으로 조작된 t 세포 Download PDF

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KR20220051401A
KR20220051401A KR1020227010925A KR20227010925A KR20220051401A KR 20220051401 A KR20220051401 A KR 20220051401A KR 1020227010925 A KR1020227010925 A KR 1020227010925A KR 20227010925 A KR20227010925 A KR 20227010925A KR 20220051401 A KR20220051401 A KR 20220051401A
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cells
gene
cell
tet2
grna
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KR1020227010925A
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조나단 알렉산더 테레트
데메트리오스 칼라이치디스
한스피터 발트너
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크리스퍼 테라퓨틱스 아게
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KR1020227010925A 2019-09-06 2020-09-04 배양 중 개선된 지속성을 갖는 유전적으로 조작된 t 세포 KR20220051401A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962897016P 2019-09-06 2019-09-06
US62/897,016 2019-09-06
US201962927764P 2019-10-30 2019-10-30
US62/927,764 2019-10-30
US202063034646P 2020-06-04 2020-06-04
US63/034,646 2020-06-04
PCT/IB2020/058280 WO2021044378A1 (en) 2019-09-06 2020-09-04 Genetically engineered t cells having improved persistence in culture

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KR20220051401A true KR20220051401A (ko) 2022-04-26

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US (1) US20210079347A1 (es)
EP (1) EP4025689A1 (es)
JP (1) JP2022547865A (es)
KR (1) KR20220051401A (es)
CN (1) CN114729325A (es)
AU (1) AU2020343516A1 (es)
BR (1) BR112022003891A2 (es)
CA (1) CA3150151A1 (es)
CO (1) CO2022002355A2 (es)
IL (1) IL290795A (es)
MX (1) MX2022002763A (es)
WO (1) WO2021044378A1 (es)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11661459B2 (en) 2020-12-03 2023-05-30 Century Therapeutics, Inc. Artificial cell death polypeptide for chimeric antigen receptor and uses thereof
JP2024500847A (ja) 2020-12-18 2024-01-10 センチュリー セラピューティクス，インコーポレイテッド 適合可能な受容体特異性を有するキメラ抗原受容体システム
WO2022238963A2 (en) * 2021-05-12 2022-11-17 Crispr Therapeutics Ag Genetically engineered immune cells targeting cd70 for use in treating solid tumors
CN117844811A (zh) * 2024-03-08 2024-04-09 上海恒润达生生物科技股份有限公司 靶向敲除CD70基因的sgRNA组合物及其应用

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6905680B2 (en) 1988-11-23 2005-06-14 Genetics Institute, Inc. Methods of treating HIV infected subjects
US6352694B1 (en) 1994-06-03 2002-03-05 Genetics Institute, Inc. Methods for inducing a population of T cells to proliferate using agents which recognize TCR/CD3 and ligands which stimulate an accessory molecule on the surface of the T cells
US5858358A (en) 1992-04-07 1999-01-12 The United States Of America As Represented By The Secretary Of The Navy Methods for selectively stimulating proliferation of T cells
US6534055B1 (en) 1988-11-23 2003-03-18 Genetics Institute, Inc. Methods for selectively stimulating proliferation of T cells
US7175843B2 (en) 1994-06-03 2007-02-13 Genetics Institute, Llc Methods for selectively stimulating proliferation of T cells
US6692964B1 (en) 1995-05-04 2004-02-17 The United States Of America As Represented By The Secretary Of The Navy Methods for transfecting T cells
US7067318B2 (en) 1995-06-07 2006-06-27 The Regents Of The University Of Michigan Methods for transfecting T cells
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6797514B2 (en) 2000-02-24 2004-09-28 Xcyte Therapies, Inc. Simultaneous stimulation and concentration of cells
JP2004500095A (ja) 2000-02-24 2004-01-08 エクサイト　セラピーズ，　インコーポレイテッド 細胞の同時の刺激および濃縮
US6867041B2 (en) 2000-02-24 2005-03-15 Xcyte Therapies, Inc. Simultaneous stimulation and concentration of cells
JP2002060786A (ja) 2000-08-23 2002-02-26 Kao Corp 硬質表面用殺菌防汚剤
EP1421177A4 (en) 2001-08-20 2006-06-07 Scripps Research Inst ZINC FINGER FASTENING DOMAINS FOR CNN
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
AU2010327998B2 (en) 2009-12-10 2015-11-12 Iowa State University Research Foundation, Inc. TAL effector-mediated DNA modification
US9428535B2 (en) 2011-10-03 2016-08-30 Moderna Therapeutics, Inc. Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
AU2013243949A1 (en) 2012-04-02 2014-10-30 Moderna Therapeutics, Inc. Modified polynucleotides for the production of biologics and proteins associated with human disease
AU2015339744B2 (en) * 2014-10-31 2021-03-25 The Trustees Of The University Of Pennsylvania Altering gene expression in CART cells and uses thereof
WO2017049166A1 (en) * 2015-09-17 2017-03-23 Novartis Ag Car t cell therapies with enhanced efficacy
AU2017261380A1 (en) * 2016-05-06 2018-11-22 Editas Medicine, Inc. Genetically engineered cells and methods of making the same
WO2018030874A1 (ko) * 2016-08-12 2018-02-15 주식회사 툴젠 조작된 면역조절요소 및 이에 의해 변형된 면역 활성
JP7215808B2 (ja) * 2016-08-12 2023-01-31 ツールゲン インコーポレイテッド 操作された免疫調節エレメントおよび変更された免疫
CA3062506A1 (en) 2017-05-12 2019-05-23 Crispr Therapeutics Ag Materials and methods for engineering cells and uses thereof in immuno-oncology
TW201936633A (zh) * 2018-02-01 2019-09-16 美商輝瑞大藥廠 標靶至cd70的嵌合抗原受體
MX2020012028A (es) 2018-05-11 2021-03-29 Crispr Therapeutics Ag Metodos y composiciones para tratar el cancer.
BR112021008041A2 (pt) 2018-11-07 2021-08-10 Crispr Therapeutics Ag terapia contra o câncer com células imunitárias anti-cd33
WO2020223537A1 (en) * 2019-05-01 2020-11-05 Pact Pharma, Inc. Compositions and methods for the treatment of cancer using a cdb engineered t cell therapy

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US20210079347A1 (en) 2021-03-18
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WO2021044378A1 (en) 2021-03-11
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