KR20190104342A - 열안정성 cas9 뉴클레아제 - Google Patents
열안정성 cas9 뉴클레아제 Download PDFInfo
- Publication number
- KR20190104342A KR20190104342A KR1020197020534A KR20197020534A KR20190104342A KR 20190104342 A KR20190104342 A KR 20190104342A KR 1020197020534 A KR1020197020534 A KR 1020197020534A KR 20197020534 A KR20197020534 A KR 20197020534A KR 20190104342 A KR20190104342 A KR 20190104342A
- Authority
- KR
- South Korea
- Prior art keywords
- nucleic acid
- protein
- sequence
- cas protein
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/50—Biochemical production, i.e. in a transformed host cell
- C12N2330/51—Specially adapted vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2016/081077 WO2018108272A1 (en) | 2016-12-14 | 2016-12-14 | Thermostable cas9 nucleases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20190104342A true KR20190104342A (ko) | 2019-09-09 |
Family
ID=57755254
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020197020534A Pending KR20190104342A (ko) | 2016-12-14 | 2016-12-14 | 열안정성 cas9 뉴클레아제 |
| KR1020197020535A Active KR102523544B1 (ko) | 2016-12-14 | 2017-08-16 | 열안정성 cas9 뉴클레아제 |
| KR1020197020536A Active KR102523543B1 (ko) | 2016-12-14 | 2017-08-16 | 열안정성 cas9 뉴클레아제 |
| KR1020197020537A Active KR102662270B1 (ko) | 2016-12-14 | 2017-12-14 | 열안정성 cas9 뉴클레아제 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020197020535A Active KR102523544B1 (ko) | 2016-12-14 | 2017-08-16 | 열안정성 cas9 뉴클레아제 |
| KR1020197020536A Active KR102523543B1 (ko) | 2016-12-14 | 2017-08-16 | 열안정성 cas9 뉴클레아제 |
| KR1020197020537A Active KR102662270B1 (ko) | 2016-12-14 | 2017-12-14 | 열안정성 cas9 뉴클레아제 |
Country Status (11)
| Country | Link |
|---|---|
| US (5) | US11242513B2 (cg-RX-API-DMAC7.html) |
| EP (4) | EP3555275A1 (cg-RX-API-DMAC7.html) |
| JP (6) | JP7182545B2 (cg-RX-API-DMAC7.html) |
| KR (4) | KR20190104342A (cg-RX-API-DMAC7.html) |
| CN (4) | CN110312792B (cg-RX-API-DMAC7.html) |
| AU (4) | AU2016432443B2 (cg-RX-API-DMAC7.html) |
| BR (3) | BR112019012155A2 (cg-RX-API-DMAC7.html) |
| CA (4) | CA3046824A1 (cg-RX-API-DMAC7.html) |
| EA (4) | EA201991443A1 (cg-RX-API-DMAC7.html) |
| PH (4) | PH12019501340A1 (cg-RX-API-DMAC7.html) |
| WO (3) | WO2018108272A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (67)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9637739B2 (en) * | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US20150166982A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting pi3k point mutations |
| EP3177718B1 (en) | 2014-07-30 | 2022-03-16 | President and Fellows of Harvard College | Cas9 proteins including ligand-dependent inteins |
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
| JP7030522B2 (ja) | 2015-05-11 | 2022-03-07 | エディタス・メディシン、インコーポレイテッド | 幹細胞における遺伝子編集のための最適化crispr/cas9システムおよび方法 |
| KR102796744B1 (ko) | 2015-06-09 | 2025-04-15 | 에디타스 메디신, 인코포레이티드 | 이식의 개선을 위한 crispr/cas-관련 방법 및 조성물 |
| GB201510296D0 (en) * | 2015-06-12 | 2015-07-29 | Univ Wageningen | Thermostable CAS9 nucleases |
| US11667911B2 (en) | 2015-09-24 | 2023-06-06 | Editas Medicine, Inc. | Use of exonucleases to improve CRISPR/CAS-mediated genome editing |
| SG11201803173VA (en) | 2015-10-23 | 2018-05-30 | Harvard College | Nucleobase editors and uses thereof |
| WO2017165826A1 (en) | 2016-03-25 | 2017-09-28 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| EP4047092B1 (en) | 2016-04-13 | 2025-07-30 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| JP7231935B2 (ja) | 2016-08-03 | 2023-03-08 | プレジデント アンド フェローズ オブ ハーバード カレッジ | アデノシン核酸塩基編集因子およびそれらの使用 |
| CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| WO2018071868A1 (en) | 2016-10-14 | 2018-04-19 | President And Fellows Of Harvard College | Aav delivery of nucleobase editors |
| CN110312792B (zh) | 2016-12-14 | 2024-04-09 | 瓦赫宁根大学 | 热稳定的Cas9核酸酶 |
| EP3555297A1 (en) | 2016-12-19 | 2019-10-23 | Editas Medicine, Inc. | Assessing nuclease cleavage |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| JP7229923B2 (ja) | 2017-01-06 | 2023-02-28 | エディタス・メディシン、インコーポレイテッド | ヌクレアーゼ切断を評価する方法 |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| EP3615672A1 (en) | 2017-04-28 | 2020-03-04 | Editas Medicine, Inc. | Methods and systems for analyzing guide rna molecules |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US10428319B2 (en) | 2017-06-09 | 2019-10-01 | Editas Medicine, Inc. | Engineered Cas9 nucleases |
| US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| EP3658573A1 (en) | 2017-07-28 | 2020-06-03 | President and Fellows of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace) |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| GB201716590D0 (en) * | 2017-10-10 | 2017-11-22 | Univ Wageningen | Thermostable cas9 nucleases with reduced off-target activity |
| AU2018352592C1 (en) | 2017-10-16 | 2025-09-25 | Beam Therapeutics, Inc. | Uses of adenosine base editors |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| CN111902536B (zh) * | 2018-02-15 | 2024-07-30 | 西格马-奥尔德里奇有限责任公司 | 用于真核基因组修饰的改造的cas9系统 |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| CA3104856A1 (en) | 2018-06-29 | 2020-01-02 | Editas Medicine, Inc. | Synthetic guide molecules, compositions and methods relating thereto |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| JP2022505440A (ja) | 2018-11-01 | 2022-01-14 | キージーン ナムローゼ フェンノートシャップ | 植物細胞におけるCRISPR/Casゲノム編集のためのデュアルガイドRNA |
| WO2020099675A1 (en) * | 2018-11-16 | 2020-05-22 | Depixus | Optimization of in vitro isolation of nucleic acids using site-specific nucleases |
| RU2712497C1 (ru) * | 2018-11-26 | 2020-01-29 | Автономная некоммерческая образовательная организация высшего образования Сколковский институт науки и технологий | Средство разрезания ДНК на основе Cas9 белка из биотехнологически значимой бактерии Clostridium cellulolyticum |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| JP2022520104A (ja) | 2019-02-15 | 2022-03-28 | シグマ-アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | Crispr/cas融合タンパク質およびシステム |
| GB2601617B (en) | 2019-03-19 | 2024-02-21 | Broad Inst Inc | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CN116694603A (zh) * | 2019-05-14 | 2023-09-05 | 深圳华大生命科学研究院 | 新型的Cas蛋白、Crispr-Cas系统及其在基因编辑领域中的用途 |
| US20220348939A1 (en) * | 2019-07-30 | 2022-11-03 | Hubei University | CRISPR-Cas system for genome editing in Zymomonas mobilis, and applications thereof |
| CN110331158B (zh) * | 2019-07-30 | 2021-09-14 | 湖北大学 | 基于运动发酵单胞菌内源CRISPR-Cas系统的多基因位点同时编辑方法及其应用 |
| CN112410234B (zh) * | 2019-08-21 | 2022-08-23 | 江南大学 | 一种多靶点编辑重组曲霉菌株的可视化筛选方法 |
| US11879134B1 (en) * | 2019-09-05 | 2024-01-23 | The Regents Of The University Of Colorado, A Body Corporate | Recombineering machinery to increase homology directed genome editing in thermophilic microbes |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
| CN111778230A (zh) * | 2020-07-17 | 2020-10-16 | 山东舜丰生物科技有限公司 | 一种适用于Cas12蛋白的缓冲系统及其应用 |
| RU2749307C1 (ru) * | 2020-10-30 | 2021-06-08 | Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт сельскохозяйственной биотехнологии" (ФГБНУ ВНИИСБ) | Новая компактная нуклеаза CAS9 II типа из Anoxybacillus flavithermus |
| CN113881652B (zh) * | 2020-11-11 | 2022-11-22 | 山东舜丰生物科技有限公司 | 新型Cas酶和系统以及应用 |
| CN114480347B (zh) * | 2022-02-21 | 2022-12-23 | 中国科学院地球化学研究所 | 一种纯化Cas12a蛋白的方法 |
| CN114934031B (zh) | 2022-05-25 | 2023-08-01 | 广州瑞风生物科技有限公司 | 新型Cas效应蛋白、基因编辑系统及用途 |
| JP7152094B1 (ja) * | 2022-06-30 | 2022-10-12 | リージョナルフィッシュ株式会社 | tracrRNAユニット、及びゲノム編集方法 |
| CN117701530A (zh) * | 2022-12-08 | 2024-03-15 | 广州瑞风生物科技有限公司 | Cas蛋白截短体、构建其的方法及其应用 |
| CN116144631B (zh) * | 2023-01-17 | 2023-09-15 | 华中农业大学 | 耐热型核酸内切酶及其介导的基因编辑系统 |
| WO2025107617A1 (zh) * | 2023-11-24 | 2025-05-30 | 华智生物技术有限公司 | CRISPR/Cas效应蛋白及系统 |
| US20250250641A1 (en) * | 2024-02-06 | 2025-08-07 | Florida State University Research Foundation, Inc. | Methods and compositions related to epigenetic editing by a methlylation-dependent cas9 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2176082B1 (es) | 2000-05-31 | 2004-01-01 | Consejo Superior Investigacion | Bacterias con la capacidad de unir metales pesados y su empleo en la detoxificacion de medios contaminados con metales pesados. |
| US7220571B2 (en) | 2000-09-28 | 2007-05-22 | Archer-Daniels-Midland Company | Escherichia coli strains which over-produce L-threonine and processes for the production of L-threonine by fermentation |
| US20040101928A1 (en) * | 2001-02-23 | 2004-05-27 | Noriko Tsutsumi | Lipolytic enzyme genes |
| US7670807B2 (en) | 2004-03-10 | 2010-03-02 | East Tennessee State Univ. Research Foundation | RNA-dependent DNA polymerase from Geobacillus stearothermophilus |
| JP2006230303A (ja) | 2005-02-25 | 2006-09-07 | Art Engineering Kk | 好熱性リパーゼ産生菌およびその利用 |
| KR100872695B1 (ko) | 2006-11-27 | 2008-12-10 | 씨제이제일제당 (주) | Gras 미생물로부터 발현된 식품안전형 호열성아라비노스 이성화효소 및 그를 이용한 타가토스의제조방법 |
| AU2008210538B2 (en) * | 2007-01-31 | 2013-06-06 | Pelican Technology Holdings, Inc. | Bacterial leader sequences for increased expression |
| EP2483400A1 (en) | 2009-09-29 | 2012-08-08 | Butamax(tm) Advanced Biofuels LLC | Improved flux to acetolactate-derived products in lactic acid bacteria |
| GB2537000C (en) * | 2012-05-25 | 2019-10-09 | Univ California | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| HRP20220803T1 (hr) | 2013-03-15 | 2022-09-30 | Cibus Us Llc | Postupci i kompozicije za povećanje efikasnosti modifikacije ciljanog gena korištenjem genske reparacije posredovane oligonukleotidom |
| FI3116305T3 (fi) | 2014-03-14 | 2024-02-08 | Cibus Us Llc | Menetelmät ja koostumukset kohdennetun geenimuunnoksen tehokkuuden lisäämiseksi käyttämällä oligonukleotidivälitteistä geeninkorjausta |
| US10725041B2 (en) | 2014-11-04 | 2020-07-28 | Versiti Blood Research Institute Foundation, Inc. | Method to bioengineer designer platelets using gene editing and stem cell methodologies |
| WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
| BR112017012765A2 (pt) | 2014-12-17 | 2018-01-16 | Du Pont | ?métodos para editar uma sequência de nucleotídeos, célula e linhagem de e. coli e método para produzir uma célula de e. coli? |
| US20160346359A1 (en) | 2015-05-01 | 2016-12-01 | Spark Therapeutics, Inc. | Adeno-associated Virus-Mediated CRISPR-Cas9 Treatment of Ocular Disease |
| BR112017024534A2 (pt) * | 2015-05-15 | 2018-07-24 | Pioneer Hi Bred Int | sistemas de rna-guia/endonuclease cas9 inovadores |
| GB201510296D0 (en) * | 2015-06-12 | 2015-07-29 | Univ Wageningen | Thermostable CAS9 nucleases |
| CN110312792B (zh) | 2016-12-14 | 2024-04-09 | 瓦赫宁根大学 | 热稳定的Cas9核酸酶 |
-
2016
- 2016-12-14 CN CN201680092098.5A patent/CN110312792B/zh active Active
- 2016-12-14 EP EP16822931.8A patent/EP3555275A1/en active Pending
- 2016-12-14 EA EA201991443A patent/EA201991443A1/ru unknown
- 2016-12-14 KR KR1020197020534A patent/KR20190104342A/ko active Pending
- 2016-12-14 US US16/469,674 patent/US11242513B2/en active Active
- 2016-12-14 CA CA3046824A patent/CA3046824A1/en active Pending
- 2016-12-14 BR BR112019012155-2A patent/BR112019012155A2/pt active Search and Examination
- 2016-12-14 JP JP2019533096A patent/JP7182545B2/ja active Active
- 2016-12-14 AU AU2016432443A patent/AU2016432443B2/en active Active
- 2016-12-14 WO PCT/EP2016/081077 patent/WO2018108272A1/en not_active Ceased
-
2017
- 2017-08-16 AU AU2017377135A patent/AU2017377135B2/en active Active
- 2017-08-16 AU AU2017377136A patent/AU2017377136B2/en active Active
- 2017-08-16 CA CA3046828A patent/CA3046828A1/en active Pending
- 2017-08-16 US US16/469,683 patent/US11976306B2/en active Active
- 2017-08-16 JP JP2019533103A patent/JP7223377B2/ja active Active
- 2017-08-16 EP EP17757728.5A patent/EP3555276A1/en active Pending
- 2017-08-16 KR KR1020197020535A patent/KR102523544B1/ko active Active
- 2017-08-16 EP EP17761030.0A patent/EP3555277A1/en active Pending
- 2017-08-16 BR BR112019012173-0A patent/BR112019012173A2/pt unknown
- 2017-08-16 EA EA201991442A patent/EA201991442A1/ru unknown
- 2017-08-16 CN CN201780086541.2A patent/CN110352241B/zh active Active
- 2017-08-16 WO PCT/EP2017/070797 patent/WO2018108339A1/en not_active Ceased
- 2017-08-16 EA EA201991440A patent/EA201991440A1/ru unknown
- 2017-08-16 KR KR1020197020536A patent/KR102523543B1/ko active Active
- 2017-08-16 US US16/469,677 patent/US11326162B2/en active Active
- 2017-08-16 CN CN201780086540.8A patent/CN110382693B/zh active Active
- 2017-08-16 CA CA3046826A patent/CA3046826A1/en active Pending
- 2017-08-16 JP JP2019532936A patent/JP7223376B2/ja active Active
- 2017-08-16 WO PCT/EP2017/070796 patent/WO2018108338A1/en not_active Ceased
- 2017-12-14 AU AU2017377792A patent/AU2017377792B2/en active Active
- 2017-12-14 JP JP2019533098A patent/JP7283698B2/ja active Active
- 2017-12-14 US US16/469,691 patent/US12071639B2/en active Active
- 2017-12-14 CN CN201780086546.5A patent/CN110431229B/zh active Active
- 2017-12-14 KR KR1020197020537A patent/KR102662270B1/ko active Active
- 2017-12-14 BR BR112019012183-8A patent/BR112019012183A2/pt active Search and Examination
- 2017-12-14 CA CA3046842A patent/CA3046842A1/en active Pending
- 2017-12-14 EA EA201991441A patent/EA201991441A1/ru unknown
- 2017-12-14 EP EP17829169.6A patent/EP3555278B1/en active Active
-
2019
- 2019-06-13 PH PH12019501340A patent/PH12019501340A1/en unknown
- 2019-06-13 PH PH12019501346A patent/PH12019501346A1/en unknown
- 2019-06-13 PH PH12019501344A patent/PH12019501344A1/en unknown
- 2019-06-13 PH PH12019501335A patent/PH12019501335A1/en unknown
-
2022
- 2022-01-14 US US17/575,952 patent/US11939605B2/en active Active
- 2022-07-29 JP JP2022121297A patent/JP2022166045A/ja not_active Withdrawn
- 2022-08-12 JP JP2022128902A patent/JP2022166170A/ja not_active Withdrawn
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11242513B2 (en) | Thermostable Cas9 nucleases | |
| US11802277B2 (en) | Thermostable Cas9 nucleases | |
| CA2989084C (en) | Thermostable cas9 nucleases |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
St.27 status event code: A-0-1-A10-A15-nap-PA0105 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| R15-X000 | Change to inventor requested |
St.27 status event code: A-3-3-R10-R15-oth-X000 |
|
| R16-X000 | Change to inventor recorded |
St.27 status event code: A-3-3-R10-R16-oth-X000 |
|
| PG1501 | Laying open of application |
St.27 status event code: A-1-1-Q10-Q12-nap-PG1501 |
|
| E13-X000 | Pre-grant limitation requested |
St.27 status event code: A-2-3-E10-E13-lim-X000 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| PA0201 | Request for examination |
St.27 status event code: A-1-2-D10-D11-exm-PA0201 |
|
| R17-X000 | Change to representative recorded |
St.27 status event code: A-3-3-R10-R17-oth-X000 |
|
| PE0902 | Notice of grounds for rejection |
St.27 status event code: A-1-2-D10-D21-exm-PE0902 |
|
| T11-X000 | Administrative time limit extension requested |
St.27 status event code: U-3-3-T10-T11-oth-X000 |
|
| T11-X000 | Administrative time limit extension requested |
St.27 status event code: U-3-3-T10-T11-oth-X000 |
|
| T11-X000 | Administrative time limit extension requested |
St.27 status event code: U-3-3-T10-T11-oth-X000 |
|
| T11-X000 | Administrative time limit extension requested |
St.27 status event code: U-3-3-T10-T11-oth-X000 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| D22 | Grant of ip right intended |
Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D22-EXM-PE0701 (AS PROVIDED BY THE NATIONAL OFFICE) |
|
| PE0701 | Decision of registration |
St.27 status event code: A-1-2-D10-D22-exm-PE0701 |