KR20180072705A - 소분자 글리코실화 방법 - Google Patents

소분자 글리코실화 방법 Download PDF

Info

Publication number
KR20180072705A
KR20180072705A KR1020187011643A KR20187011643A KR20180072705A KR 20180072705 A KR20180072705 A KR 20180072705A KR 1020187011643 A KR1020187011643 A KR 1020187011643A KR 20187011643 A KR20187011643 A KR 20187011643A KR 20180072705 A KR20180072705 A KR 20180072705A
Authority
KR
South Korea
Prior art keywords
sucrose
sucrose phosphorylase
phosphorylase
ascorbic acid
glucosyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
KR1020187011643A
Other languages
English (en)
Korean (ko)
Inventor
라마 크리쉬나 구디민치
베른트 니데츠키
Original Assignee
아시브 게엠베하
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 아시브 게엠베하 filed Critical 아시브 게엠베하
Publication of KR20180072705A publication Critical patent/KR20180072705A/ko
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1066—Sucrose phosphate synthase (2.4.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01007—Sucrose phosphorylase (2.4.1.7)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
KR1020187011643A 2015-09-25 2016-09-22 소분자 글리코실화 방법 Withdrawn KR20180072705A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15186888 2015-09-25
EP15186888.2 2015-09-25
PCT/EP2016/072586 WO2017050920A1 (en) 2015-09-25 2016-09-22 Method for small molecule glycosylation

Publications (1)

Publication Number Publication Date
KR20180072705A true KR20180072705A (ko) 2018-06-29

Family

ID=54251987

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020187011643A Withdrawn KR20180072705A (ko) 2015-09-25 2016-09-22 소분자 글리코실화 방법

Country Status (7)

Country Link
US (1) US20180291410A1 (https=)
EP (1) EP3353311B1 (https=)
JP (1) JP7000327B2 (https=)
KR (1) KR20180072705A (https=)
CN (1) CN108350474B (https=)
ES (1) ES2886572T3 (https=)
WO (1) WO2017050920A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108913641B (zh) * 2018-06-29 2021-02-02 浙江工业大学 一种重组大肠杆菌及其应用
CN109988778B (zh) * 2019-05-14 2020-10-27 南京工业大学 一种蔗糖磷酸化酶基因及其应用
CN111172128A (zh) * 2020-01-21 2020-05-19 浙江工业大学 一种蔗糖磷酸化酶在制备2-O-α-D-葡萄糖基-L-抗坏血酸中的应用
CN113980932B (zh) * 2021-07-15 2023-08-01 暨南大学 一种定点突变的α-葡萄糖苷酶
CN115786388B (zh) * 2022-12-26 2025-01-14 暨南大学 生产2-O-α-D-吡喃葡萄糖基-L-抗坏血酸的基因工程菌及其构建方法与应用
CN116144625A (zh) * 2023-02-24 2023-05-23 杭州佳嘉乐生物技术有限公司 重组蔗糖磷酸化酶及其催化糖基化反应的方法
CN120230742B (zh) * 2025-06-03 2025-07-29 山东三元生物科技股份有限公司 一种固定化细胞及其制备方法和2-O-α-D-吡喃葡萄糖基-L-抗坏血酸的制备方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2832848B2 (ja) 1989-10-21 1998-12-09 株式会社林原生物化学研究所 結晶２―Ｏ―α―Ｄ―グルコピラノシル―Ｌ―アスコルビン酸とその製造方法並びに用途
JP3134235B2 (ja) 1991-10-23 2001-02-13 株式会社林原生物化学研究所 ２−Ｏ−α−Ｄ−グルコピラノシル−Ｌ−アスコルビン酸高含有物の製造方法
US20020006418A1 (en) * 1998-10-13 2002-01-17 John Kung Composition to enhance permeation of topical skin agents
JP4043312B2 (ja) 2002-08-06 2008-02-06 株式会社林原生物化学研究所 ２−Ｏ−α−Ｄ−グルコピラノシル−Ｌ−アスコルビン酸の製造方法
KR20140043890A (ko) * 2011-03-25 2014-04-11 프로테로, 인크. 조절된 유전자 발현 시스템 및 그의 작제물
WO2014060452A1 (en) * 2012-10-18 2014-04-24 Universiteit Gent A thermostable sucrose and sucrose-6'-phosphate phosphorylase
CN103122341B (zh) * 2013-01-08 2015-05-13 江南大学 一种麦芽糊精底物特异性提高的环糊精糖基转移酶及其制备方法
CN104531629B (zh) * 2014-12-16 2017-07-21 江南大学 一种提高aa‑2g转化率的环糊精葡萄糖基转移酶突变体

Also Published As

Publication number Publication date
US20180291410A1 (en) 2018-10-11
CN108350474A (zh) 2018-07-31
ES2886572T3 (es) 2021-12-20
EP3353311A1 (en) 2018-08-01
EP3353311B1 (en) 2021-06-16
JP7000327B2 (ja) 2022-02-04
JP2018535693A (ja) 2018-12-06
CN108350474B (zh) 2022-06-28
WO2017050920A1 (en) 2017-03-30

Similar Documents

Publication Publication Date Title
US10683525B2 (en) Method for producing 2-O-glyceryl-alpha-D-glucopyranoside
KR20180072705A (ko) 소분자 글리코실화 방법
JP6479084B2 (ja) 一連の糖転移酵素およびその応用
KR20090010024A (ko) 유전자 조작법에 의한 덱스트란수크라제 ｄｓｒ－ｓ의 신규변이체의 제조
JP4043015B2 (ja) 糖転移物の製造方法
CN113265433B (zh) 双功能碳苷糖基转移酶及其应用
WO1995034642A1 (en) Novel transferase and amylase, process for producing the enzymes,use thereof, and gene coding for the same
CN109415747B (zh) 一种酶改质甜菊糖的制备方法和制备用酶及应用
Chen et al. Changing the donor cofactor of bovine α1, 3-galactosyltransferase by fusion with UDP-galactose 4-epimerase: more efficient biocatalysis for synthesis of α-Gal epitopes
CN112725313B (zh) 一种β-半乳糖苷酶的制备及其应用
EP2186904B1 (en) Method for producing glucose derivatives
JP2017123844A (ja) 配糖体の製造方法
US5804426A (en) Recombinant cyclodextran glucanotransferase mutants
KR101978565B1 (ko) 루미노코커스 브로미 유래 풀루란 분해 효소 및 이의 용도
DK182217B1 (en) Enzymatic n-acetyllactosaminylation of a terminal galactose in its 6-position
EP0263955A2 (en) Process for the production of panosyl derivatives
CN111635894B (zh) 粉蝶霉素糖基转移酶sGT1及应用
CN121271818A (zh) 一种蔗糖磷酸化酶突变体及其应用
KR101655237B1 (ko) 고정화된 아밀레이즈를 이용한 말토덱스트린의 연속 제조방법
CN117413056A (zh) 用于果寡糖的优化生产的经修饰的果聚糖蔗糖酶
KR101707928B1 (ko) CGTase 변이효소를 이용한 고순도 말토헵타오스의 제조방법
KR20070105563A (ko) 바실러스 세레우스 균주로부터 유래한 당전이효소, 그의유전자 및 그들의 제조방법
JP2005210925A (ja) グルコシル基の転移方法

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20180424

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
N231 Notification of change of applicant
PN2301 Change of applicant

Patent event date: 20191023

Comment text: Notification of Change of Applicant

Patent event code: PN23011R01D

PC1203 Withdrawal of no request for examination
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid