KR20130019329A

KR20130019329A - Medium Composition of Mushroom Containing Golden Ingredients and Cultivation Method thereof

Info

Publication number: KR20130019329A
Application number: KR1020110081699A
Authority: KR
Inventors: 전창호
Original assignee: 전창호
Priority date: 2011-08-16
Filing date: 2011-08-16
Publication date: 2013-02-26

Abstract

PURPOSE: A culture medium composition containing gold ions is provided to be used for cultivating all kinds of mushrooms. CONSTITUTION: A culture medium is manufactured by the following steps: mixing 30 wt% scattered straws (or peel of soybean, sawdust of black oak, sawdust of poplar tree, sawdust of Douglas fir, sawdust of poplar tree), 10 wt% bean-curd dregs, 15 wt% rice bran, 5 wt% dried poultry waste powder, 3 wt% calcium hydroxide powder, 2 wt% plaster powder, 5 wt% cotton, 5 wt% arrow-root root powder, 15 wt% microalgae powder, 15 wt% coffee sediment powder, 5 wt% residue of herb medicine powder; diluting 1-10ppm of 20-35nano colloidal gold having concentration of 1000ppm with water 65 wt% of the culture medium weight to make the concentration be 1-10ppm; aging the mixture by dispersing and mixing at intervals of two days for 15 days; and sterilizing the mixture with steam at 100-120°C for 5-10 hours. A cultivation method using the culture medium comprises the following steps: filling up a plastic bottle or plastic bag with about 700gm of the manufactured culture media and injecting mushroom starters; culturing the starters at 20-21°C in 67% humidity for 15-35 days; growing at 14-15°C in 85% humidity after germinating at 16-17°C in 95% humidity for 10 days. The mushrooms include pleurotus eryngii, lentinus edodes, button mushroom(Agaricus bisporus (J. Lange)Sing), oyster mushroom(Pleurotus ostreatus), Auricularia auricula(Hook) Underw, Hericium erinaceum, Hydnum erinaceum Fr., Flammulina velutipes.

Description

Medium composition of mushroom containing golden component and its cultivation method

The present invention relates to a medium composition of the mushroom containing the golden (Au) component and a cultivation method thereof, and more specifically, Pleurotus eryngii, shiitake mushroom, mushroom oyster, oyster mushroom, oyster mushroom, roe deer mushroom, enoki mushroom, basidiomycete Allied medium including all mushrooms of microorganism belonging to Basidiomycets, crushed crest (or oak sawdust, cottonwood sawdust, cypress sawdust, poplar sawdust), bean waste (or soybean hulls, soybean meal, wheat flour, corn powder, Bran), rice bran, chicken dung powder, calcium hydroxide powder, gypsum powder, cotton wool, powdered root powder, microalgae powder, coffee ground powder, herbal powders, and 1000ppm of colloidal state of about 20-35 nanometers in water It relates to a culture medium culture and culture method characterized by the addition of dispersed culture culture, mushroom strain culture, grown mushrooms containing the golden (Au) ion.

In general, mushrooms are classified microorganisms belonging to Basidiomycets, and are natural vegetable-friendly, high-grade foods, and are widely used as health foods due to their high pharmacological effects such as anticancer and antioxidant effects.

Conventional methods for cultivating mushrooms have been to puncture oaks, insert mushroom spawns, and block the holes with cotton and wait for six months to a year or more in a certain place suitable for humidity and temperature. It is difficult to grow the medium according to the mushroom type using wood sawdust, corn powder, soybean hull, bran, etc. in the greenhouse to adjust the temperature and humidity. This has been.

The present invention is to cultivate and grow mushroom strains as an integrated medium of mushrooms, shiitake mushrooms, mushrooms, mushrooms, oyster mushrooms, soybean mushrooms, roe deer mushrooms, oyster mushrooms, all mushrooms of microorganisms belonging to Basidiomtcets. In addition, the purpose is to produce a mushroom containing a differentiated functional gold (Au) ion by adding a gold (Au) ion to the medium of the present invention.

The present invention is an integrated medium of all mushrooms of microorganisms belonging to Pleurotus eryngii mushroom, shiitake mushroom, mushroom mushroom, oyster mushroom, oyster mushroom, roe deer mushroom, enoki mushroom, eggplant mushroom, ganoderma lucidum, basidiomycets 30% by weight of crested rice (or oak sawdust, cottonwood sawdust, cypress sawdust, poplar sawdust), bean curd (or soybean hulls, soybean meal, soybean meal, corn powder, bran) 10% by weight, rice bran 15%, chicken dung powder 5 Weight%, Calcium Hydroxide Powder 3%, Gypsum Powder 2%, Cotton Wool 5%, Root Root Powder 5%, Microalgae Powder 15%, Coffee Ground Powder 5%, Herb Powder 5% 1,000 ppm of gold (Au) ionic liquid in a colloidal state of about 20-35 nanometers was diluted in 1-10 ppm of 65% water in the total weight ratio of the medium, and dispersed and mixed once every two days at 30 ° C. for about 15 days. Steam sterilization at 100-120 ° C for 5-10 hours A seed of a mushroom through a completed by separating medium has a feature that the culture, the composition of the medium and cultivation methods to ensure growth by gold (Au) ions are detected in the mushroom mathematics.

The present invention relates to a medium composition of a golden (Au) -containing mushroom and a method for cultivating the same, and including soy mushroom, shiitake mushroom, mushroom mushroom, oyster mushroom, soybean mushroom, roe mushroom, and oyster mushroom, etc. It was confirmed that the effect of detecting the golden (Au) ions contained in the mushroom cultured and grown mushroom culture with the composition of the integrated medium and the culture medium of the present invention can be used integrally of all the mushrooms of the microorganism belonging to.

The present invention relates to a medium composition of the mushroom containing the golden (Au) component and a cultivation method thereof, which will be described below with reference to examples.

Example 1

As an integrated medium for easy cultivation and growth of mushroom fungi, the first experimental task is to check the process of cultivation and growth by easily absorbing the golden (Au) ions and cultivating and growing soybean sprouts and bean sprouts. 300 ppm of purified water and 1 liter of purified water each added 5 ppm of the concentration of 1,000 ppm of colloidal gold (Au) nano solution with pink (the solution of the nanoscale of gold is the color of pink color). The sprouts and host sprouts were grown by spraying water with -7 times golden ions.

On the 4th day, the shoots sprouted, and on the 7-8th day, the sprouts and the stems of the host sprouts, which grew about 3-5cm, began to grow in a pink color, and visually confirmed that the golden (Au) ions were easily absorbed.

Example 2 (mushroom fungi integrated medium)

30% by weight of crushed crest (or oak sawdust, cottonwood sawdust, cypress sawdust, poplar sawdust), soy bean curd (or soybean hull, soybean meal, wheat bran, corn powder, bran), rice bran 15 weight %, Chicken dung powder 5 wt%, Calcium hydroxide powder 3 wt%, Gypsum powder 2 wt%, Cotton cotton powder 5 wt%, Root root powder 5 wt%, Microalgae powder 15 wt%, Coffee ground powder 5 wt% Disperse and mix 5% by weight of herbal residue powder, and dilute 1-10ppm of 1,000-ppm gold (Au) solution in a colloidal state of 20-35 nanoparticles in about 65% water by weight of the medium. After aging by dispersing once every two days for about 15 days at 5-10 hours steam sterilization at 100-120 ℃ to complete the composition of the integrated medium.

Example 3 (cultivation and growth of mushrooms)

The integrated medium of Example 2 was placed in a plastic bottle and a plastic bag, and about 700 gr of the fungus and the fungus were inoculated, respectively, and incubated for 15-35 days at a temperature of 20-21 ° C. and a humidity of 67%. The mushrooms were harvested at a temperature of about 95 to 10 days at 95 ° C, a growth temperature of about 14 to 10 ° C, and about 6 to 10 days at a humidity of 85%.

After pulverizing each of the harvested Pleurotus eryngii and Thirsty Mushroom, the ions of gold (Au) were quantitatively analyzed, and the results were 4.5 and 5 ppm respectively.

Claims

Crushed rice cake (or oak sawdust, cottonwood sawdust, cypress sawdust, poplar sawdust) 30 Wt%, soybean meal (or soybean hull, soybean meal, wheat flour, corn powder, bran) 10% by weight, rice bran 15%, chicken dung powder 5% by weight, calcium hydroxide powder 3% by weight, gypsum powder 2% by weight, cotton wool 5% %, 5% by weight of root powder, 15% by weight of microalgae powder, 5% by weight of ground peat powder, 5% by weight of Chinese herbal remnant powder, and 1000ppm concentration of gold in 20-35 nano-colloidal state. Au) ion solution was diluted by 1-10ppm dilution in water at 65% of the weight of the medium, dispersed and mixed at 30 ° C for 15 days at 2 days intervals, aged, and then steam sterilized at 100-120 ° C for 5-10 hours. Integrated medium composition of the finished mushroom.

The method according to claim 1, wherein about 700 grams of medium is filled in a plastic bottle or a plastic bag, and mushroom spawn is incubated for 15-35 days at a temperature of 20-21 ° C. and a humidity of 67%. The germination temperature is 16-17 ° C., After culturing for 10 days at 95% humidity, mushrooms grown at 14-15 ° C. and 85% humidity as a growth temperature contain golden (Au) ions.