CN102617212A - Sterilizable strain activating agent for edible fungi and application of sterilizable strain activating agent - Google Patents

Sterilizable strain activating agent for edible fungi and application of sterilizable strain activating agent Download PDF

Info

Publication number
CN102617212A
CN102617212A CN2012100821061A CN201210082106A CN102617212A CN 102617212 A CN102617212 A CN 102617212A CN 2012100821061 A CN2012100821061 A CN 2012100821061A CN 201210082106 A CN201210082106 A CN 201210082106A CN 102617212 A CN102617212 A CN 102617212A
Authority
CN
China
Prior art keywords
actication
activating agent
sulfate
edible
sterilizing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN2012100821061A
Other languages
Chinese (zh)
Other versions
CN102617212B (en
Inventor
李莉
徐国华
盖晓宇
陈飞
周建树
冀宝营
筴殿国
柴林山
邓春海
孙立梅
李杨
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LIAONING SCIENTIFIC ACADEMY OF MICROBIOLOGY
Original Assignee
LIAONING SCIENTIFIC ACADEMY OF MICROBIOLOGY
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LIAONING SCIENTIFIC ACADEMY OF MICROBIOLOGY filed Critical LIAONING SCIENTIFIC ACADEMY OF MICROBIOLOGY
Priority to CN201210082106.1A priority Critical patent/CN102617212B/en
Publication of CN102617212A publication Critical patent/CN102617212A/en
Application granted granted Critical
Publication of CN102617212B publication Critical patent/CN102617212B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Mushroom Cultivation (AREA)

Abstract

The invention discloses a sterilizable strain activating agent, which is made of copper sulfate, zinc sulfate, ferrous sulfate, manganese sulfate, magnesium sulfate, dipotassium phosphate, calcium superphosphate, carbamide and glucose. The activating agent can be mixed and used with edible fungus culturing materials and can be also sprayed for use at the later stage of edible fungi after mushrooms grow out. The sterilizable strain activating agent is simple in utilization technique and easy to be accepted by peasants, has active ingredients for starting of edible fungus hypha, is reasonable in proportion and resistant to high pressure and high temperature, and can be directly applied to mixing, bagging and sterilizing and also be sprayed for used at the later stage after mushrooms grow out. The sterilizable strain activating agent enables rough nutrition in the edible fungus culturing materials to be rapidly turned into nutritional ingredients usable for the edible fungus hypha, quick starting is advantageous, pollution is reduced, culturing time is greatly shortened, mushrooms can grow out ahead of time, and benefits of mushroom farmers can be increased. Further, the active ingredients contained in the activating agent are resistant to high pressure and high temperature, and the sterilizable strain activating agent is simple and convenient in application and can be directly mixed for application.

Description

Edible mushrooms is with actication of culture agent and the application thereof of can sterilizing
Technical field
The present invention relates to a kind of edible fungus species acvator technical field; Relating in particular to a kind of edible mushrooms with actication of culture agent and the application thereof of can sterilizing, is a kind of available nutrient preparation that promotes that edible fungus species starts, makes bacterial classification ramp on substratum, breeding fast.
Background technology
Present edible mushrooms has become an important industry of China, agricultural, be only second in the plant husbandry grain, cotton, oil, really, dish, rank the 6th.According to related data statistics, China Edible Fungi main forces are about 3,000 ten thousand people, and ultimate production accounts for 70% of world's total amount, and export volume accounts for more than 80% of the Asia total volume of exports, about 50,000,000,000 yuan of the gross output value, and ultimate production is approximately with annual about 15% speed increase in recent years.Not only quality is tender for edible mushrooms, unique flavor, and is rich in nutritions such as polysaccharide, protein, multiple amino acids, VITAMINs, Mierocrystalline cellulose, mineral substance, and lower fat is fabulous heath food.Along with improving constantly of living standards of the people, more and more deep to the understanding of edible mushrooms, edible mushrooms has become people's important foodstuffs source.But edible mushrooms belongs to macro fungi, and its mycelial growth, breeding, differentiation need the long time.With the mushroom is example, and after mushroom fruiting bag (specification the is 17*55) inoculation, mycelia just can be covered with the bacterium bag in 50-60 days, and this makes mycelial growth inhomogeneous, influences fruiting time, output and quality, thereby has also influenced the economic benefit of mushroom farming.
The short long agent of disclosed at present edible fungus species, as 201010138339.X number, the preparation method of growth promoter for edible fungi and application thereof; 200610022415.4 number, environment-friendly edible fungus bacteria-treating and short long agent and preparation and method of use; Such growth-promoting modifying agent all is used for promptly during fruiting, using after the edible fungus species cultivation, makes such growth promoter can't promote the hypha of edible fungus growth, can not reduce hypha of edible fungus and grow, breeds, breaks up the used time.Not seeing at present the research contents of relevant sterilization property edible fungus species acvator aspect as yet openly reports.
Summary of the invention
The objective of the invention is many defectives based on the prior art existence; The application method of a kind of edible mushrooms with can sterilize actication of culture agent and this actication of culture agent proposed; This actication of culture agent is to be the nutrient prescription of staple to start growth, bacterial classification is started fast and breeds, and makes that thick nutritive ingredient is converted into the available nutritive ingredient of hypha of edible fungus fast in the culture material to occupy advantage; Reduce and pollute, increase output.
Plan of the present invention is: the first is the nutrient that bacterial classification starts provides rational proportion, comprises carbon source (glucose), moderate-element (calcium salt) trace element etc.; The effective constituent that comprises in its two this actication of culture agent is high pressure resistant, high temperature resistant; It three can make in the culture medium of edible fungus thick nutrition be converted into the available nutritive ingredient of hypha of edible fungus fast, starts fast and occupies advantage, reduces and pollutes, and shorten incubation time greatly, makes the fruiting phase in advance, increases benefit; Its four be use easy, directly spice application.The concrete technical scheme that proposes based on above-mentioned purpose the present invention is:
Edible mushrooms is characterized in that with the actication of culture agent of can sterilizing: this actication of culture agent its proportioning by weight is:
Copper sulfate 0.01-0.03kg; Zinc sulfate 0.2-0.8kg; Ferrous sulfate 0.2-0.5kg;
Manganous sulfate 0.1-0.35kg; Sal epsom 3-4kg; Potassium hydrogenphosphate 2.5-3.5kg;
Superphosphate of lime 4.0-6.5kg; Urea 18.0-20kg; Glucose 66-72kg;
Copper sulfate, zinc sulfate, ferrous sulfate, manganous sulfate, sal epsom, potassium hydrogenphosphate, superphosphate of lime, urea, glucose are ground, process 40-80 order particle, packing, sealing are promptly processed edible mushrooms with the actication of culture agent of can sterilizing behind the mixing according to the above ratio.
The range of application of acvator of the present invention is: the above-mentioned actication of culture agent of sterilizing can be used with the culture medium of edible fungus mixing, can use at the edible mushroom later spray again;
Spice uses: above-mentioned edible mushrooms is added water with the actication of culture agent of can sterilizing process suspension-s, suspension-s is joined in the culture medium of edible fungus according to 0.15~0.3% weight ratio again, later stage spice, pack, high pressure or normal-pressure sterilization are pressed routine operation;
Spray and use: the actication of culture agent of can sterilizing is diluted with water to the diluting soln of 0.05~0.1W%, is sprayed directly on edible mushrooms and takes off on bag fruiting bacterium bag, every day 1~3 time, each fruiting bag sprinkling amount: 100~150ml.
The present invention compared with prior art has following characteristics:
1, edible mushrooms of the present invention can make hypha of edible fungus start fast with the actication of culture agent of can sterilizing, and for bacterial classification starts the nutrient that rational proportion is provided, comprises carbon source (glucose), moderate-element (calcium salt) trace element etc.; Make the available nutrient preparation of bacterial classification ramp on substratum, breeding;
2, this acvator range of application is wide in range; Can be used for edible mushroomss such as mushroom, auricularia auriculajudae, flat mushroom, Pleurotus eryngii; Both can add mixing in the culture medium of edible fungus, sterilization application in proportion to, can also use, nutrition can be provided comprehensively at the edible mushroom later spray; Improve the edible mushrooms quality, increase output; Use this technology can shorten the hypha of edible fungus incubation time greatly, make the fruiting phase in advance;
3, can make the thick nutrition in the culture medium of edible fungus be converted into the available nutritive ingredient of hypha of edible fungus fast, start the advantage that occupies fast, shorten incubation time, reduce and pollute;
4, the effective constituent that comprises in the acvator of the present invention is high pressure resistant, high temperature resistant; Use easyly, directly spice is used, and this acvator use technology is simple, is accepted by the peasant easily.
Embodiment
Embodiment 1: edible mushrooms is with the preparation of the actication of culture agent of can sterilizing
1, actication of culture agent preparation:
Prescription 1: copper sulfate 0.01kg; Zinc sulfate 0.25kg; Ferrous sulfate 0.3kg; Glucose 69.0kg; Superphosphate of lime 4.5kg;
Sal epsom 3.5kg; Potassium hydrogenphosphate 3.0kg; Urea 19.0kg; Manganous sulfate 0.2kg.
Prescription 2: copper sulfate 0.02kg; Zinc sulfate 0.5kg; Ferrous sulfate 0.2kg; Glucose 68.0kg; Cross
Calcium phosphate 4.8kg; Sal epsom 3kg; Potassium hydrogenphosphate 2.7kg; Urea 18.0kg; Manganous sulfate 0.1kg.
Prescription 3: copper sulfate 0.03kg; Zinc sulfate 0.75kg; Ferrous sulfate 0.4kg; Glucose 71.0kg; Superphosphate of lime 6.0kg;
Sal epsom 4kg; Potassium hydrogenphosphate 3.5kg; Urea 20.0kg; Manganous sulfate 0.3kg.
2, preparation method:
Copper sulfate, zinc sulfate, ferrous sulfate, glucose, superphosphate of lime, sal epsom, potassium hydrogenphosphate, urea and the manganous sulfate of above-mentioned dosage are ground; Process 40-80 order particle; After mixing, packing, sealing (anti-moisture absorption caking) are promptly processed edible mushrooms with the actication of culture agent of can sterilizing.
Embodiment 2: edible mushrooms is with the application method of actication of culture agent in mushroom is produced of can sterilizing
Actication of culture agent range of application of the present invention: can add sterilization application in the champignon compost by a certain percentage to, promptly use, can also use,, increase output for edible fungus species provides nutrition at mushroom fruiting later spray with the champignon compost mixing.
1. spice uses: process suspension-s but the agent of above-mentioned sterilizing edible fungi actication of culture is dissolved in water, again with suspension-s according in 0.15% the weight proportion adding cultivating champignon material, later stage spice, pack, high pressure or normal-pressure sterilization are pressed routine operation.
2. spray to use: with above-mentioned actication of culture agent dilute with water, be made into the acvator weight content and be 0.1% dilute aqueous solution, diluent is sprayed directly on the fruiting bacterium bag after mushroom takes off bag, every day 1 time, the sprinkling amount is: each fruiting bag 100~150ml.
Below enumerate table 1, table 2 and be the correlation data of mycelial growth and acvator effect of increasing production during the present invention implements.
Table 1: edible mushrooms is with the short hypha of edible fungus growth result table of the actication of culture agent of can sterilizing
Figure BSA00000690755800041
The subordinate list explanation:
1. mycelia is covered with bacterium bag (specification is high for 17cm bag folding footpath * 55cm bag), and required fate refers to that 1000 bacterium bag inoculation back to bacterium bags all cover with needed fate.
2. mycelial growth pollutes the bag number refers to that 1000 bacterium bags take place altogether during all covering with to the bacterium bag after the inoculation pollution bag number.
3. pollution rate refers to the shared per-cent of contaminated bacteria bacterium bag in 1000 bags of experimental bacteria bags.
4. the parallel appearance of 1.2.3. finger to finger test in showing.
5. fill a prescription 1 in the table, prescription 2, prescription 3 refer to the three assembly sides that provide among the embodiment 1.
Table 2: edible mushrooms is with the actication of culture agent effect of increasing production table of can sterilizing
Figure BSA00000690755800051
The subordinate list explanation:
1. the quantity of buddingging refers to that cultivating champignon stops the 3rd day quantity percentage ratio of buddingging of rod that shakes behind the bacterium for the first time, promptly occurs the number of mushroom flower bud in 100 fruiting bacterium bags (model is the high 55cm of bag folding footpath 17cm * bag).
2. the edible mushroom later stage uses and refers to not use during the bacterium behind mushroom fruiting the 3rd damp mushroom; Repairing the bacterium time is 12 days; The 12nd day rod that shakes, the number of buddingging of the 3rd day record in rod back that shake, the mushroom flower bud number that promptly occurs in 100 fruiting bags (model is the high 55cm of bag folding footpath 17cm * bag).
3. output refers to the ultimate production of 1000 shiitake cultivation bags (model is the high 55cm of bag folding footpath 17cm * bag) after the season.
4. the parallel appearance of 1.2.3. finger to finger test in showing.
5. fill a prescription 1 in the table, prescription 2, prescription 3 refer to the three assembly sides that provide among the embodiment 1.
Show through a large amount of tests: in mushroom production, use edible mushrooms with the actication of culture agent of can sterilizing, the spawn culture time average shortens 10.3 days, and pollution rate reduces by 4.8%, and the number of buddingging improves 38%~48%, and output improves 0.1kg/ bag~0.16kg/ bag.

Claims (2)

1. edible mushrooms is characterized in that with the actication of culture agent of can sterilizing: this actication of culture agent its proportioning by weight is:
Copper sulfate 0.01-0.03kg; Zinc sulfate 0.2-0.8kg; Ferrous sulfate 0.2-0.5kg;
Manganous sulfate 0.1-0.35kg; Sal epsom 3-4kg; Potassium hydrogenphosphate 2.5-3.5kg;
Superphosphate of lime 4.0-6.5kg; Urea 18.0-20kg; Glucose 66-72kg;
Copper sulfate, zinc sulfate, ferrous sulfate, manganous sulfate, sal epsom, potassium hydrogenphosphate, superphosphate of lime, urea, glucose are ground, process 40-80 order particle, packing, sealing are promptly processed edible mushrooms with the actication of culture agent of can sterilizing behind the mixing according to the above ratio.
2. according to the application method of the described edible mushrooms of claim 1 with the actication of culture agent of can sterilizing; The range of application that it is characterized in that this acvator is: the above-mentioned actication of culture agent of sterilizing can be used with the culture medium of edible fungus mixing, can use at the edible mushroom later spray again;
Spice uses: above-mentioned edible mushrooms is added water with the actication of culture agent of can sterilizing process suspension-s, suspension-s is joined in the culture medium of edible fungus according to 0.15~0.3% weight ratio again, later stage spice, pack, high pressure or normal-pressure sterilization are pressed routine operation;
Spray and use: the actication of culture agent of can sterilizing is diluted with water to the diluting soln of 0.05~0.1W%, is sprayed directly on edible mushrooms and takes off on bag fruiting bacterium bag, every day 1~3 time, each fruiting bag sprinkling amount: 100~150ml.
CN201210082106.1A 2012-03-22 2012-03-22 Sterilizable strain activating agent for edible fungi and application of sterilizable strain activating agent Expired - Fee Related CN102617212B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201210082106.1A CN102617212B (en) 2012-03-22 2012-03-22 Sterilizable strain activating agent for edible fungi and application of sterilizable strain activating agent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201210082106.1A CN102617212B (en) 2012-03-22 2012-03-22 Sterilizable strain activating agent for edible fungi and application of sterilizable strain activating agent

Publications (2)

Publication Number Publication Date
CN102617212A true CN102617212A (en) 2012-08-01
CN102617212B CN102617212B (en) 2014-04-16

Family

ID=46557486

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201210082106.1A Expired - Fee Related CN102617212B (en) 2012-03-22 2012-03-22 Sterilizable strain activating agent for edible fungi and application of sterilizable strain activating agent

Country Status (1)

Country Link
CN (1) CN102617212B (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105272443A (en) * 2015-09-23 2016-01-27 百色市右江区永乐三宝农产品有限公司 Se-enriched Baise black fungus production medium additive and application method
CN106358757A (en) * 2016-08-29 2017-02-01 惠州市嘉程驾校有限公司 Lentinula edodes planting method
CN107980475A (en) * 2017-12-05 2018-05-04 罗成喜 A kind of implantation methods of mushroom
CN112409031A (en) * 2020-09-30 2021-02-26 上海市农业科学院 Microelement complex solution for promoting agaricus bisporus hypha growth and improving yield
CN112586272A (en) * 2020-12-21 2021-04-02 贺州市田投农业发展有限公司 Method for efficiently cultivating pleurotus citrinopileatus by mulberry twigs
CN114680000A (en) * 2021-11-10 2022-07-01 上海市农业科学院 Straw mushroom culture material additive and preparation method and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1418462A (en) * 2001-11-14 2003-05-21 中国科学院沈阳应用生态研究所 Special fertilizer for cultivating edible mushroom, the prepn. method and application thereof
CN101611719A (en) * 2009-07-23 2009-12-30 武汉联合创业可再生能源有限公司 Plant growth promoter and preparation method thereof
CN101805225A (en) * 2010-03-31 2010-08-18 吴洪生 Method for preparing growth promoter for edible fungi and application thereof
CN101823906A (en) * 2010-04-08 2010-09-08 辽宁省微生物科学研究院 Culture activator and application thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1418462A (en) * 2001-11-14 2003-05-21 中国科学院沈阳应用生态研究所 Special fertilizer for cultivating edible mushroom, the prepn. method and application thereof
CN101611719A (en) * 2009-07-23 2009-12-30 武汉联合创业可再生能源有限公司 Plant growth promoter and preparation method thereof
CN101805225A (en) * 2010-03-31 2010-08-18 吴洪生 Method for preparing growth promoter for edible fungi and application thereof
CN101823906A (en) * 2010-04-08 2010-09-08 辽宁省微生物科学研究院 Culture activator and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
马璐等: "无机盐、维生素与植物生长调节剂对绣球菌菌丝生长的影响", 《菌物研究》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105272443A (en) * 2015-09-23 2016-01-27 百色市右江区永乐三宝农产品有限公司 Se-enriched Baise black fungus production medium additive and application method
CN106358757A (en) * 2016-08-29 2017-02-01 惠州市嘉程驾校有限公司 Lentinula edodes planting method
CN107980475A (en) * 2017-12-05 2018-05-04 罗成喜 A kind of implantation methods of mushroom
CN112409031A (en) * 2020-09-30 2021-02-26 上海市农业科学院 Microelement complex solution for promoting agaricus bisporus hypha growth and improving yield
CN112586272A (en) * 2020-12-21 2021-04-02 贺州市田投农业发展有限公司 Method for efficiently cultivating pleurotus citrinopileatus by mulberry twigs
CN114680000A (en) * 2021-11-10 2022-07-01 上海市农业科学院 Straw mushroom culture material additive and preparation method and application thereof

Also Published As

Publication number Publication date
CN102617212B (en) 2014-04-16

Similar Documents

Publication Publication Date Title
CN102210233B (en) Production method of calcium-enriched edible fungi and formula of culture medium
CN102617212B (en) Sterilizable strain activating agent for edible fungi and application of sterilizable strain activating agent
CN103571766B (en) Liquid microbial leaf fertilizer, and production method and applications thereof
CN104557339A (en) Preparation method of soluble fish protein ammonia acid fertilizer
CN102165895A (en) Fungus stick and method used for cultivating mushroom or agaric by utilizing ramulus mori
CN102696458A (en) Edible fungus compost, production method thereof and edible fungus culture process
CN104429624A (en) Oyster mushroom cultivation method
CN103880543A (en) Culture medium for cultivating straw mushrooms and preparation method thereof
CN107473824A (en) A kind of complex microorganism liquid fertilizer and preparation method thereof
CN104291895A (en) Organic liquid fertilizer special for syzygium samarangense, and preparation technology and application method thereof
CN107602268A (en) The special organic fertilization fertilizer of rape
CN103719541A (en) Mushroom dreg feed and preparation method thereof
CN102040405A (en) Fish protein polypeptide biological bacterial fertilizer
CN104987156A (en) Lyophyllum fumosurn culture medium using fermented bran and method for cultivating lyophyllum fumosurn
CN103396226A (en) Pleurotus eryngii culture medium
CN105110856A (en) Lentinula edodes culture medium
CN103936468B (en) A kind of corn composite fertilizer
CN103708970B (en) Special compound biological fertilizer for tobacco
CN103739391B (en) A kind of leaf vegetables special composite biological is fertile
CN107118060A (en) Biological organic fertilizer and preparation method thereof
CN104862247A (en) Liquid medium of composite microbial agent and preparation method thereof
CN102173910A (en) Marine byproduct fermentation concentration composite fertilizer and production method thereof
CN103044130B (en) Agricultural microbial agent and making method
CN103621310B (en) Utilize mountain grass and the wild beef gill fungus of mushroom bran success domesticating and cultivating
CN110304968A (en) A kind of edible fungus culture medium modifying agent

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140416

Termination date: 20160322