CN102617212B - Sterilizable strain activating agent for edible fungi and application of sterilizable strain activating agent - Google Patents
Sterilizable strain activating agent for edible fungi and application of sterilizable strain activating agent Download PDFInfo
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- CN102617212B CN102617212B CN201210082106.1A CN201210082106A CN102617212B CN 102617212 B CN102617212 B CN 102617212B CN 201210082106 A CN201210082106 A CN 201210082106A CN 102617212 B CN102617212 B CN 102617212B
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Abstract
The invention discloses a sterilizable strain activating agent, which is made of copper sulfate, zinc sulfate, ferrous sulfate, manganese sulfate, magnesium sulfate, dipotassium phosphate, calcium superphosphate, carbamide and glucose. The activating agent can be mixed and used with edible fungus culturing materials and can be also sprayed for use at the later stage of edible fungi after mushrooms grow out. The sterilizable strain activating agent is simple in utilization technique and easy to be accepted by peasants, has active ingredients for starting of edible fungus hypha, is reasonable in proportion and resistant to high pressure and high temperature, and can be directly applied to mixing, bagging and sterilizing and also be sprayed for used at the later stage after mushrooms grow out. The sterilizable strain activating agent enables rough nutrition in the edible fungus culturing materials to be rapidly turned into nutritional ingredients usable for the edible fungus hypha, quick starting is advantageous, pollution is reduced, culturing time is greatly shortened, mushrooms can grow out ahead of time, and benefits of mushroom farmers can be increased. Further, the active ingredients contained in the activating agent are resistant to high pressure and high temperature, and the sterilizable strain activating agent is simple and convenient in application and can be directly mixed for application.
Description
Technical field
The present invention relates to a kind of edible fungus species activator technical field, relating in particular to a kind of edible mushrooms with can sterilizing culture activator and application thereof, is a kind of available nutrient preparation that promotes that edible fungus species starts, makes bacterial classification ramp on substratum, breeding fast.
Background technology
Current edible mushrooms has become an important industry of China, agricultural, be only second in plant husbandry grain, cotton, oil, really, dish, rank the 6th.According to related data statistics, China Edible Fungi main forces are about 3,000 ten thousand people, and ultimate production accounts for 70% of world's total amount, and export volume accounts for the more than 80% of the Asia total volume of exports, approximately 50,000,000,000 yuan of the gross output values, and ultimate production is approximately with the speed increase of annual 15% left and right in recent years.Not only quality is tender for edible mushrooms, unique flavor, and is rich in the nutritions such as polysaccharide, protein, multiple amino acids, VITAMIN, Mierocrystalline cellulose, mineral substance, and lower fat, is fabulous heath food.Along with improving constantly of living standards of the people, more and more deep to the understanding of edible mushrooms, edible mushrooms has become people's important foodstuffs source.But edible mushrooms belongs to macro fungi, the time that its mycelial growth, breeding, differentiation need to be longer.Take mushroom as example, and after mushroom fruiting bag (specification is 17*55) inoculation, within 50-60 days, mycelia just can be covered with bacterium bag, and this makes mycelial growth inhomogeneous, affects fruiting time, output and quality, thereby has also affected the economic benefit of mushroom agriculture.
The short long agent of existing published edible fungus species, as No. 201010138339.X, the preparation method of growth promoter for edible fungi and application thereof; 200610022415.4 number, environment-friendly edible fungus bacteria-treating and short long agent and preparation and application thereof; Such growth-promoting modifying agent is used after all cultivating for edible fungus species during fruiting, makes such growth promoter cannot promote hypha of edible fungus growth, can not reduce hypha of edible fungus and grow, breeds, breaks up the time used.The research contents that has not yet to see relevant sterilizing edible fungus species activator aspect is openly reported.
Summary of the invention
The object of the invention is the many defects that exist based on prior art, a kind of edible mushrooms is proposed with can sterilizing culture activator and the application method of this culture activator, this culture activator is to start the nutrient formula that growth is main component, can make bacterial classification start fast and breed, making thick nutritive ingredient rapid conversion in culture material is that the available nutritive ingredient of hypha of edible fungus occupies advantage, reduce and pollute, increase output.
Design of the present invention is: the first, for bacterial classification starts the nutrient that rational proportion is provided, comprises carbon source (glucose), moderate-element (calcium salt) trace element etc.; The effective constituent comprising in its two this culture activator is high pressure resistant, high temperature resistant; Three can to make in culture medium of edible fungus thick nutrition rapid conversion be the available nutritive ingredient of hypha of edible fungus for it, starts fast and occupy advantage, reduces and pollute, and greatly shorten incubation time, makes the fruiting phase in advance, increases benefit; It four is that application is easy, and directly spice is applied.The concrete technical scheme proposing based on above-mentioned purpose the present invention is:
Edible mushrooms with can sterilizing culture activator, is characterized in that: this culture activator by weight its proportioning is:
Copper sulfate 0.01-0.03kg; Zinc sulfate 0.2-0.8kg; Ferrous sulfate 0.2-0.5kg;
Manganous sulfate 0.1-0.35kg; Magnesium sulfate 3-4kg; Dipotassium hydrogen phosphate 2.5-3.5kg;
Calcium superphosphate 4.0-6.5kg; Urea 18.0-20kg; Glucose 66-72kg;
Copper sulfate, zinc sulfate, ferrous sulfate, manganous sulfate, magnesium sulfate, dipotassium hydrogen phosphate, calcium superphosphate, urea, glucose are ground, make 40-80 order particle, mix according to the above ratio rear packing, sealing and make edible mushrooms with can sterilizing culture activator.
The range of application of activator of the present invention is: above-mentioned can use with culture medium of edible fungus mixing by sterilizing culture activator, can use at edible mushroom later spray again;
Spice is used: above-mentioned edible mushrooms is made to suspension with can sterilizing culture activator adding water, then suspension is joined in culture medium of edible fungus according to 0.15～0.3% weight ratio, later stage spice, pack, high pressure or normal-pressure sterilization operate routinely;
Spray to use: can sterilizing culture activator be diluted with water to the diluting soln of 0.05～0.1W%, be sprayed directly on the de-bag of edible mushrooms fruiting bacterium bag every day 1～3 time, each fruiting bag sprinkling amount: 100～150ml.
The present invention compared with prior art has following features:
1, edible mushrooms of the present invention, with can making hypha of edible fungus start fast by sterilizing culture activator, for bacterial classification starts the nutrient that rational proportion is provided, comprises carbon source (glucose), moderate-element (calcium salt) trace element etc.; Make the available nutrient preparation of bacterial classification ramp on substratum, breeding;
2, this activator range of application is wide in range, can be used for the edible mushroomss such as mushroom, auricularia auriculajudae, flat mushroom, Pleurotus eryngii, both mixing in culture medium of edible fungus, sterilizing application can have been added in proportion to, can also use at edible mushroom later spray, nutrition can be provided comprehensively, improve edible mushrooms quality, increase output; Use this technology can greatly shorten hypha of edible fungus incubation time, make the fruiting phase in advance;
3, can make the thick nutrition rapid conversion in culture medium of edible fungus is the available nutritive ingredient of hypha of edible fungus, starts fast the advantage that occupies, and shortens incubation time, reduces and pollutes;
4, the effective constituent comprising in activator of the present invention is high pressure resistant, high temperature resistant; Apply easy, directly spice application, this activator operation technique is simple, easily by peasant, is accepted.
Embodiment
Embodiment 1: preparation that can sterilizing culture activator for edible mushrooms
1, culture activator preparation:
Formula 1: copper sulfate 0.01kg; Zinc sulfate 0.25kg; Ferrous sulfate 0.3kg; Glucose 69.0kg; Calcium superphosphate 4.5kg;
Magnesium sulfate 3.5kg; Dipotassium hydrogen phosphate 3.0kg; Urea 19.0kg; Manganous sulfate 0.2kg.
Formula 2: copper sulfate 0.02kg; Zinc sulfate 0.5kg; Ferrous sulfate 0.2kg; Glucose 68.0kg; Cross
Calcium phosphate 4.8kg; Magnesium sulfate 3kg; Dipotassium hydrogen phosphate 2.7kg; Urea 18.0kg; Manganous sulfate 0.1kg.
Formula 3: copper sulfate 0.03kg; Zinc sulfate 0.75kg; Ferrous sulfate 0.4kg; Glucose 71.0kg; Calcium superphosphate 6.0kg;
Magnesium sulfate 4kg; Dipotassium hydrogen phosphate 3.5kg; Urea 20.0kg; Manganous sulfate 0.3kg.
2, preparation method:
The copper sulfate of above-mentioned dosage, zinc sulfate, ferrous sulfate, glucose, calcium superphosphate, magnesium sulfate, dipotassium hydrogen phosphate, urea and manganous sulfate are ground, make 40-80 order particle, after mixing, edible mushrooms use is made in packing, sealing (anti-moisture absorption caking) can sterilizing culture activator.
Embodiment 2: edible mushrooms is used can the application method of sterilizing culture activator in Lentnus edodes
Culture activator range of application of the present invention: can add by a certain percentage sterilizing application in champignon compost to, use with champignon compost mixing, can also use at mushroom fruiting later spray, for edible fungus species provides nutrition, increase output.
1. spice is used: above-mentioned can sterilizing edible fungi culture activator being dissolved in water made to suspension, then suspension is added in cultivating champignon material according to 0.15% weight proportion, later stage spice, pack, high pressure or normal-pressure sterilization operate routinely.
2. spray to use: by above-mentioned culture activator dilute with water, be made into activator weight content and be 0.1% dilute aqueous solution, diluent is sprayed directly on the fruiting bacterium bag after the de-bag of mushroom, every day 1 time, sprinkling amount is: each fruiting bag 100～150ml.
Below enumerate table 1, table 2 and be the correlation data of mycelial growth and activator effect of increasing production in the invention process.
Table 1: edible mushrooms is with urging hypha of edible fungus growth result table by sterilizing culture activator
Subordinate list explanation:
1. mycelia is covered with bacterium bag (specification is that 17cm bag folding footpath * 55cm bag is high), and required number of days refers to after 1000 bacterium bags inoculations all to cover with needed number of days to bacterium bag.
2. mycelial growth pollutes the pollution bag number that bag number refers to have altogether during 1000 bacterium bags all cover with to bacterium bag after inoculation generation.
3. pollution rate refers to the shared per-cent of contaminated bacteria bacterium bag in 1000 bags of experimental bacteria bags.
4. 1.2.3. finger to finger test Duplicate Samples in showing.
5. three group of formula of filling a prescription 1 in table, providing in formula 2, formula 3 finger embodiment 1.
Table 2: edible mushrooms is used can sterilizing culture activator effect of increasing production table
Subordinate list explanation:
1. Squaring number refers to that cultivating champignon stops the 3rd day Squaring number percentage ratio of rod that shakes after bacterium for the first time, in 100 fruiting bacterium bags (model is the high 55cm of bag folding footpath 17cm * bag), occurs the number of mushroom flower bud.
2. the edible mushroom later stage is used and refers to not during bacterium, use after mushroom fruiting the 3rd damp mushroom, repairing the bacterium time is 12 days, the 12nd day rod that shakes, the number of buddingging of the 3rd day record after the rod that shakes, the mushroom flower bud number occurring in 100 fruiting bags (model is the high 55cm of bag folding footpath 17cm * bag).
3. output refers to the ultimate production of 1000 shiitake cultivation bags (model is the high 55cm of bag folding footpath 17cm * bag) after the season.
4. 1.2.3. finger to finger test Duplicate Samples in showing.
5. three group of formula of filling a prescription 1 in table, providing in formula 2, formula 3 finger embodiment 1.
By a large number of experiments show that: use edible mushrooms with can sterilizing culture activator in Lentnus edodes, spawn culture time average shortens 10.3 days, and pollution rate reduces by 4.8%, and the number of buddingging improves 38%～48%, and output improves 0.1kg/ bag～0.16kg/ bag.
Claims (1)
1. application method that can sterilizing culture activator for edible mushrooms, is characterized in that: this culture activator by weight its proportioning is:
Copper sulfate 0.01-0.03kg; Zinc sulfate 0.2-0.8kg; Ferrous sulfate 0.2-0.5kg;
Manganous sulfate 0.1-0.35kg; Magnesium sulfate 3-4kg; Dipotassium hydrogen phosphate 2.5-3.5kg;
Calcium superphosphate 4.0-6.5kg; Urea 18.0-20kg; Glucose 66-72kg;
Copper sulfate, zinc sulfate, ferrous sulfate, manganous sulfate, magnesium sulfate, dipotassium hydrogen phosphate, calcium superphosphate, urea, glucose are ground, make 40-80 order particle, mix according to the above ratio rear packing, sealing and make edible mushrooms with can sterilizing culture activator;
The range of application of described activator is: above-mentioned can use with culture medium of edible fungus mixing by sterilizing culture activator, can use at edible mushroom later spray again;
Spice is used: above-mentioned edible mushrooms is made to suspension with can sterilizing culture activator adding water, then suspension is joined in culture medium of edible fungus according to 0.15～0.3% weight ratio, later stage spice, pack, high pressure or normal-pressure sterilization operate routinely;
Spray to use: can sterilizing culture activator be diluted with water to the diluting soln of 0.05～0.1W%, be sprayed directly on the de-bag of edible mushrooms fruiting bacterium bag every day 1～3 time, each fruiting bag sprinkling amount: 100～150ml.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105272443A (en) * | 2015-09-23 | 2016-01-27 | 百色市右江区永乐三宝农产品有限公司 | Se-enriched Baise black fungus production medium additive and application method |
| CN106358757A (en) * | 2016-08-29 | 2017-02-01 | 惠州市嘉程驾校有限公司 | Lentinula edodes planting method |
| CN107980475A (en) * | 2017-12-05 | 2018-05-04 | 罗成喜 | A kind of implantation methods of mushroom |
| CN112409031A (en) * | 2020-09-30 | 2021-02-26 | 上海市农业科学院 | Microelement complex solution for promoting agaricus bisporus hypha growth and improving yield |
| CN112586272A (en) * | 2020-12-21 | 2021-04-02 | 贺州市田投农业发展有限公司 | Method for efficiently cultivating pleurotus citrinopileatus by mulberry twigs |
| CN114680000A (en) * | 2021-11-10 | 2022-07-01 | 上海市农业科学院 | Straw mushroom culture material additive and preparation method and application thereof |
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| CN1418462A (en) * | 2001-11-14 | 2003-05-21 | 中国科学院沈阳应用生态研究所 | Special fertilizer for cultivating edible mushroom, the prepn. method and application thereof |
| CN101611719A (en) * | 2009-07-23 | 2009-12-30 | 武汉联合创业可再生能源有限公司 | Plant growth promoter and preparation method thereof |
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