KR20100083203A - Method for producing skin whitening products using the king oyster mushroom - Google Patents

Abstract

PURPOSE: A method for manufacturing whitening product using King oyster mushroom is provided to reduce production cost. CONSTITUTION: A method for manufacturing whitening cosmetic product using King oyster mushroom comprises: a step of mixing glycerin, propylene glycol, EDTA-2Na, methyl paraben, and aqua; a step of adding stearic acid, kalcol 6870, aralcel 165, grape seed oil, olive oil, liquid paraffin, GTCC, and propyl paraben and mixing; a step of adding aqua, and carbopol 940; a step of isolating ergosterol peroxide; and a step of adding Na-Hyaluronate(0.1%) and perfume and mixing.

Description

Method for producing skin whitening products using the King Oyster Mushroom}

The present invention relates to a method for producing a whitening functional product using a Pleurotus eryngii, more specifically, a method for producing a whitening functional transdermal cosmetic (essence, sunscreen) and an oral product (granule, capsule) using a Pleurotus eryngii as a raw material It is about.

As the Pleurotus eryngii is a pesticide-free and pollution-free, safe material with no toxicity, it not only reduces the toxicity required for commercialization and the cost of clinical experiments, but also utilizes the functional properties that have been identified so far, and it is very high as a functional cosmetic material. do. Therefore, the new mushroom mushroom parcel, which is easy to secure and supply raw materials and has a high price competitiveness, is developed as a functional cosmetic material, so that the functional cosmetics can be diversified, and the price of the fresh pine mushroom market is increased, and the fresh pine mushroom is currently in poor business. As it is judged that the managerial stability of the growers can be satisfied, the technology and products to be developed in this project are very important.

Recently, a major concern in women's beauty is the skin of black stools, blemishes, freckles, dark circles and the like, whitening of the skin is more important than ever before. In general, dark skin, blemishes, freckles, dark circles, etc. are considered to be caused by melanocytes are stimulated by ultraviolet light stimulation, hormonal balance abnormalities, genetic factors, etc. As a result, biosynthetic melanin pigment is deposited on the skin. have. Conventionally, melanin inhibitors, such as L-ascorbic acid or its derivatives, hydroquinone derivatives, placental extracts, kojic acid, arbutin and the like, are formulated into cosmetics by applying it to the skin such as dark skin, blemishes, freckles and dark circles. Treatment and improvement methods have been mainly used.

However, in the case of the conventional cosmetics as described above, the effective ingredient is erased over time due to sweating and the like, and thus there is a problem that the whitening effect is very insufficient, and the effect is exerted only on the coated part. That is, when used as a cosmetic, it is necessary to re-apply several times a day, or to apply the cosmetics to the whole body against darkening or blemishes, freckles, dark circles, etc. of the whole body, and the burden or inconvenience of the user is inherent. In addition, the active ingredient is a skin irritant, it is restricted to use or has an unusual smell, there is a lack of stability due to precipitation flocculation, and also decomposed by external stimulation such as pH fluctuations on the skin surface caused by light, heat or sweat. In addition, problems such as deterioration and deterioration have been pointed out.

Because of improving the problems with the existing cosmetics as described above, oral ingestion of whitening foods or whitening drugs are also being developed. For example, there is a combination of vitamin C in this kind of food, vitamin C has a problem that the stability is not enough to satisfy the whitening effect. In addition, whitening food (Japanese Patent Laid-Open Publication No. Hei 6-16685), cosmetics, food and bathing agents (Japanese Patent Laid-Open Publication No. Hei 10-287525) and isoflavones containing koji acids are added. Oral whitening agents and whitening foods (Japanese Patent Laid-Open Publication No. Hei 11-269066), whitening foods containing proanthocyanidins and glutathione as active ingredients (Japanese Patent Laid-Open Publication No. 2000-60482), and the like. The products developed so far could not be expected to have a sufficient whitening effect, and depending on the component has the problem that there are concerns about unpredictable side effects due to oral intake. Judging from this situation comprehensively, the development of a food or oral whitening agent with high stability while having a sufficient whitening effect is urgently required.

The present invention has a technical problem to provide a method for producing a whitening functional transdermal cosmetics (essences, sunscreen) and oral products (granules, capsules) using a new mushroom as a raw material.

In order to solve the above technical problem, the present invention provides a method for producing a whitening functional cosmetics or products using a new pine mushroom having the following technical characteristics.

The whitening functional cosmetics manufacturing method using the Pleurotus eryngii according to the present invention comprises the steps of: mixing Glycerin, Propylene glycol, EDTA-2Na, Methyl paraben, Aqua; Adding and mixing Stearic acid, Kalcol 6870, Aralcel 165, Grape seed oil, Olive oil, Liquid paraffin, GTCC, Propyl paraben; Adding Aqua, Carbopol 940 and mixing; In addition, the ergosterol peroxide is isolated from Pleurotus eryngii and Na-Hyaluronate (0.1%), the step of mixing with Perfume; and characterized in that it comprises a.

The whitening functional cosmetics manufacturing method using the Pleurotus eryngii according to the present invention comprises the steps of: mixing Glycerin, Propylene glycol, EDTA-2Na, Methyl paraben, Aqua; Adding by mixing Stearic acid, Aralcel 165, Parsol 5000, Cutina E-24, Propyl paraben, GTCC, Parsol 1789, Amphisol, BHT, Tea, Parsol MCX; Adding Aqua, Carbopol 940 and mixing; Adding Aqua, Mirasun Tiw 60, and TiO ₂ to mix; And, the step of separating the ergosterol peroxide from the matsutake mushroom and adding with Perfume and mixing; characterized in that it comprises a.

A method for producing a whitening oral product using the mushrooms according to the present invention comprises the steps of extracting hot mushrooms powder, bokbunja, Schisandra chinensis, wolfberry, casualty, earthenware; Adding vitamin C and vitamin E to hot water extracts; And, by kneading the mixture granulation; characterized in that comprises a.

A method for producing a whitening oral product using a fungus according to the present invention comprises: mixing a fungus powder, a ergosterol peroxide concentrated powder isolated from a fungus, vitamin C, and vitamin E; And, the step of putting the mixture into the capsule; characterized in that comprises a.

According to the present invention, since the high value-added whitening functional product can be manufactured using the Pleurotus eryngii discarded as a by-product, an advantageous effect can be expected for economic gain creation.

Hereinafter, look at the present invention based on Examples and Test Examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1 Isolation of Ergosterol Peroxide

The filtrate obtained by immersing 3 kg of Pleurotus eryngii dry powder and drying it three times was immersed in 10 l of 100% ethanol for 3 days, and the filtrate was partitioned with hexane, and the hexane layer concentrate was hexane / ethyl. Silica gel column chromatography was performed while gradually increasing the concentration of ethyl acetate under the solvent condition of acetate to obtain a fraction. The sixth fraction was subjected to silica gel column chromatography while gradually increasing the ether concentration under hexane / ether solvent conditions. The sixth fraction was recrystallized from petroleum ether. 0.6 g was separated. The separated compounds were identified as ergosterol peroxide (C ₂₈ H ₄₄ O ₃ ) by the proton NMR spectrum and carbon NMR spectrum.

Test 1 Inhibitory effect of ergosterol peroxide on melanogenesis: In-flight test

Melanogenesis inhibitory effect was evaluated by in-flight test while observing melanogenesis by applying the diluted solution of ergosterol peroxide to the cells using B16F10 melanomer cells. Divided into 6 steps by concentration in the concentration range of 0 ~ 3.0㎍ / ㎖ by injecting 80μL of cell suspension into the plate containing ergosterol peroxide, and producing melanin in melanoma cells while incubating for 72 hours in 37 ℃ CO ₂ incubate Whether or not and the degree of production was observed.

As a result, as shown in Figure 1, ergosterol peroxide showed a tendency to significantly inhibit melanogenesis, especially at a concentration of 3.0㎍ / ㎖ showed a complete inhibition. These results suggest that ergosterol peroxide has a whitening mechanism that directly inhibits the formation of melanin, unlike the general whitening mechanism that inhibits the formation of melanin by inhibiting tyrosinase activity.

[Test Example 2] Inhibitory Effects of Ergosterol Peroxide on Melanogenesis: Animal Testing

Black mice (5 weeks old, males) were preliminarily bred for one week with commercial feed (CJ Co., Ltd.), and then the back of the black mouse was shaved about half a day. It was irradiated for 5 minutes, and it was irradiated for 5 minutes at 1.0 kV intensity on the 2nd and 3rd day. Subsequently, a group coated with ergosterol peroxide cream was made daily at a predetermined site and raised for two weeks, and then examined by animal tests for differences in the degree of melanin pigmentation induced by UV induction of these black mice. The ergosterol peroxide cream used in this test was prepared by adding 1% ergosterol peroxide to 25% alcohol, polyoxyethylene (25 mole) hardened 2.0 castor oil, antioxidants, preservatives, fragrances, etc. A water-based composition was prepared by mixing 5.0% of sodium hexametaphosphate with an appropriate amount of ion-exchanged water, and then mixing and solubilizing these two types of compositions to prepare a transdermal coating composition (lotion). ergosterol peroxide) was not added.

As a result of the test, ergosterol peroxide was shown to significantly inhibit melanin production, as shown in FIG. These results suggest that ergosterol peroxide inhibits the activity of tyrosinase and thus inhibits the production of melanin, suggesting the possibility of the development of sunscreen.

[Test Example 3] Whitening effect of ergosterol peroxide by oral administration: Animal test

Black mice (5 weeks old, males) were preliminarily bred for one week with commercial feed (CJ Co., Ltd.), and then half of the black mouse's back was removed. It was irradiated for 5 minutes, and it was irradiated for 5 minutes at 1.0 kV intensity on the 2nd and 3rd day. Subsequently, after breeding for 2 weeks with a low vitamin C feed (200.0 mg / kg), these black mice were divided into 3 groups of 7 animals per group so as not to cause a difference in the degree of melanin pigmentation induced by UV induction. As a result, the test group freely fed the low-vitamin C feed with 1% ergosterol peroxide, the control group consumed low vitamin C feed, and the positive control added 1% vitamin C to the low vitamin C feed. One meal was taken.

Diet group Compound Amount Control - - Positive control Ascorbic acid One% Test group Ergosterol peroxide One%

Four weeks after the start of the test diet, the degree of pigmentation of the skin was significantly improved (reliably improved as compared to the positive control group), moderate improvement (pigmentation to the same degree as the positive control group), and slightly improved (the control and positive control groups). Medium pigmentation), no change was judged based on four criteria. The results are shown in the following [Table 2]. As can be seen, the oral administration of ergosterol peroxide significantly improved the deposition of melanin pigment, and the markedly improved sample is shown in FIG. 3.

Results of Whitening Evaluation in Black Mouse group Significantly improved ^{1 )} Medium improvement ^{2 )} Slightly improved ^{3 )} No change ^{4 )} Control 0 0 0 7 Positive control 0 One 4 2 Test group 3 2 2 0 ¹⁾ Significantly improved: Significantly improved compared to the positive control group
²⁾ Medium improvement: Pigmentation of the same degree as the positive control group
³⁾ Slight improvement: Medium pigmentation between control and positive control
⁴⁾ No change: pigmentation of the same degree as the control

These results confirm that the melanin pigmentation can be suppressed by oral administration of ergosterol peroxide, which is evidence that the whitening effect is also obtained by oral administration rather than by transdermal application. Therefore, it suggests that there is also a possibility of developing oral (content) whitening cosmetics rather than transdermal (outer).

Test Example 4 Whitening effect of ergosterol peroxide by transdermal application: human test

25% alcohol, polyoxyethylene (25 mol) hardening 2.0 1% of the test substance (ergosterol peroxide) was added to castor oil, antioxidants, preservatives and fragrances to make an alcoholic composition, and glycerin 5.0% and sodium hexametaphosphate After mixing with ion-exchanged water to make an aqueous composition, these two compositions were mixed and solubilized to prepare a transdermal coating composition (lotion) for human testing.

 As a test method, 20 panelists were selected and exposed to sunlight for 2 hours, 2 hours a day for 2 hours a day from 11 am to 1 pm in summer. After 5 days from the day of exposure to sunlight, the composition was applied once a morning and evening for 5 weeks. As a criterion, it is divided into A that pigmentation becomes hardly noticeable, B which pigmentation becomes very thin, C which pigmentation becomes thinner, and D which does not change. The test was conducted on the control group and the test group as 15 ~ 11 persons were B or higher, and 2 ~ 10 or 6 persons were B or higher, and 3 or 5 or lower were B or higher.

As a result of the test, as shown in [Table 3], the test with ergosterol peroxide resulted in the first grade, whereas in the no addition group, the fourth grade was determined, and as shown in FIG. I could confirm that. Therefore, ergosterol peroxide is considered to have excellent whitening effect even when applied to human percutaneous skin.

External test results Test compound Compound weight (%) effect Ergosterol peroxide 1.0 Grade 1 No additives - Grade 4

Example 2 Preparation of Percutaneous Whitening Functional Cosmetics

1. Whitening essence

The first sample made based on the first recipe based on the Sepig gel was strongly sticky and had a problem in touch, requiring a change of raw materials. Twenty-one of the 30 panelists cited stickiness, and the cause was due to the lack of emulsifier, so the problem was solved by modifying the recipe with a common emulsification base to reduce oily and reduce sticky touch. Could. As shown in FIG. 5, the oil content was significantly reduced by significantly modifying one species of Sepig gel such as Stearic acid, Kalcol 6870, Aralcel 165, Grape seed oil, Olive oil, Liquid paraffin, GTCC, Propyl paraben. In addition, panelists said that the fragrance in the first sample was rather strong, and recently, cosmetic trends tended to favor low fragrances. Could reduce. In order to improve the whitening effect, the amount of ergosterol peroxide was adjusted to 0.5% of the primary sample to 1% of the high whitening effect in animal and human tests. As a result of modifying the recipe as described above was able to obtain a slightly improved effect on the skin absorbency.

2. Whitening suncream

The recipe of the Whiting suncream was designed according to the modified final recipe of the whitening essence, as shown in FIG. A Step and C Step are the same as Whiting essence, and in Step B, Cutina E, which is related to UV protection, excludes 3 kinds of ingredients such as Kalcol 6870, Grape seed oil and Olive oil, which are related to whitening, wrinkle improvement, and transparent skin. 6 species including -24, Amphisol, BHT, Parsol 5000, Parsol 1789, and Parsol MCX were added, and instead of excluding Na-Hyaluronate (0.1%) of D Step, 3 species including Aqua, Mirasun Tiw 60, and TiO ₂ were added. Whiting suncream recipe with UV protection index (SPF) 40 was completed. The characteristic of this whitening suncream is that it is a sunscreen functional cosmetic that has a whitening effect that effectively blocks UV-B. Another advantage is that it was developed as a makeup base.

The manufacturing process of the whitening essence and the whitening sunscreen as described above is as shown in FIG.

Example 3 Preparation of Oral (Contents) Whitening Functional Cosmetics

1.Whitening granule

In the previous animal test, the whitening effect by oral administration was found to be very excellent, and therefore, the oral (content) whitening functional cosmetics for granules were developed, and the developed recipe is shown in FIG. 8.

The five characters used in the recipe of FIG. 8 are good for skin and tonic, and their characteristics are as follows. Bokbunja is a rosaceae medicinal herb made from unripe fruit of bokbunja strawberry, it has the effect of skin shine, oil well (premature ejaculation), infertility, cold, pneumonia, cough, urinary disease, blue blood, adult disease prevention. Schisandra chinensis is a fruit of Schisandra chinensis, which has five flavors: sweet, sour, bitter, salty, and spicy. It has the effects of fatigue, blood pressure control, hypoglycemia, diarrhea, cardiovascular function, and gastric secretion control. There is this. Goji berries belong to the branch family, the fruit of the Goji berries, egg-shaped, long oval, red ripening properties, tonic, antipyretic, liver function protection, renal function, antioxidant effect, diabetes, and prevention of adult diseases. Casualties belong to the Buttercup family, and there are five types, but the medicine used is the seed of the snake. Efficacy is skin disease, tonic, infertility, vaginitis, anti-inflammatory, itching, rheumatism, dry stomach, diuresis, etc. Tosa is a ume flower, a seed of the ginseng, an annual plant. Nourishment, tonic, oil well, low back pain, diabetes, bleeding, jaundice, nocturia, blemishes, freckles, black mushrooms are effective.

The whitening granules of the recipe of Figure 8 can be mass-produced in the same process as in Figure 9, specifically, Pleurotus eryngii powder and Oja put 10 times the water (during drying) compared to the raw materials, the liquid extractor for 36 hours at 97 ℃ Atmospheric hot water extract (2.3 Brix) is added to 20% (weight ratio) of Pleurotus eryngii powder, a small amount of vitamin C and vitamin E, and then kneaded sufficiently to prepare a granulation process. A prototype of the whitening granules produced by this manufacturing process is shown in FIG. 10.

2. Whitening capsule

In the previous animal test, the whitening effect by oral administration was shown to be very excellent, so the capsule oral use (content) was developed to whitening functional cosmetics, the developed recipe is shown in FIG. The manufacturing process of the whitening capsule is shown in Figure 12, specifically, Pleurotus eryngii powder as a main raw material Ergosterol peroxide concentrated powder and a small amount of vitamin C and vitamin E, and then put into the capsule is prepared by the process. A prototype of the whitening capsule manufactured by this manufacturing process is shown in FIG. 13.

1 shows melanogenesis inhibitory activity of ergosterol peroxide.

Figure 2 shows the whitening effect by the application of ergosterol peroxide.

Figure 3 shows the whitening effect by oral administration of ergosterol peroxide.

Figure 4 shows the whitening effect of ergosterol peroxide in human skin.

Figure 5 shows the manufacturing recipe of the whitening essence with a transdermal whitening functional cosmetics.

Figure 6 shows the recipe of whitening suncream as a transdermal whitening functional cosmetics.

Figure 7 shows a manufacturing process of the whitening essence and whitening suncream (dermal dermal) according to the present invention.

8 shows a recipe for preparing a whitening granule according to the present invention.

Figure 9 shows a manufacturing process of the whitening granules (oral).

10 prepares a prototype of a whitening granule.

11 shows a recipe for producing a whitening capsule according to the present invention.

12 shows a manufacturing process diagram of the whitening capsule (oral).

13 shows a prototype of a whitening capsule.

Claims (4)

Mixing Glycerin, Propylene glycol, EDTA-2Na, Methyl paraben, Aqua; Adding and mixing Stearic acid, Kalcol 6870, Aralcel 165, Grape seed oil, Olive oil, Liquid paraffin, GTCC, Propyl paraben; Adding Aqua, Carbopol 940 and mixing; And, Separating ergosterol peroxide from Pleurotus eryngii and adding Na-Hyaluronate (0.1%) and Perfume to mix; Whitening functional cosmetics manufacturing method using a matsutake mushroom, characterized in that comprises a. Mixing Glycerin, Propylene glycol, EDTA-2Na, Methyl paraben, Aqua; Adding by mixing Stearic acid, Aralcel 165, Parsol 5000, Cutina E-24, Propyl paraben, GTCC, Parsol 1789, Amphisol, BHT, Tea, Parsol MCX; Adding Aqua, Carbopol 940 and mixing; Adding Aqua, Mirasun Tiw 60, and TiO ₂ to mix; And, Separating ergosterol peroxide from Pleurotus eryngii and adding it with Perfume to mix; Whitening functional cosmetics manufacturing method using a matsutake mushroom, characterized in that comprises a. Hydrothermal extraction of Pleurotus eryngii powder, bokbunja, Schisandra chinensis, wolfberry, casualty, earthenware; Adding vitamin C and vitamin E to hot water extracts; And, Kneading the mixture to granulate; Method for producing a whitening functional oral product using a matsutake mushroom, characterized in that comprises a. Mixing Pleurotus eryngii powder, concentrated powder of ergosterol peroxide isolated from Pleurotus eryngii, vitamin C, vitamin E; And, Placing the mixture in a capsule; Method for producing a whitening functional oral product using a matsutake mushroom, characterized in that comprises a.

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