KR20100059789A - 배아줄기세포 배양을 위한 자동화 방법 및 장치 - Google Patents

배아줄기세포 배양을 위한 자동화 방법 및 장치 Download PDF

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KR20100059789A
KR20100059789A KR1020107002003A KR20107002003A KR20100059789A KR 20100059789 A KR20100059789 A KR 20100059789A KR 1020107002003 A KR1020107002003 A KR 1020107002003A KR 20107002003 A KR20107002003 A KR 20107002003A KR 20100059789 A KR20100059789 A KR 20100059789A
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cells
cell
medium
population
inhibitor
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나다니엘 비어즐리
파이트 베르겐달
메건 피츠제럴드
크리스틴 다이그
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셀룰러 다이내믹스 인터내셔널, 인코포레이티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/07—Dosage or metering devices therefore
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Wood Science & Technology (AREA)
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  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
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  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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KR1020107002003A 2007-06-29 2008-06-30 배아줄기세포 배양을 위한 자동화 방법 및 장치 Ceased KR20100059789A (ko)

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US94701307P 2007-06-29 2007-06-29
US60/947,013 2007-06-29

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KR1020157036767A Division KR20160005142A (ko) 2007-06-29 2008-06-30 배아줄기세포 배양을 위한 자동화 방법 및 장치

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KR1020107002003A Ceased KR20100059789A (ko) 2007-06-29 2008-06-30 배아줄기세포 배양을 위한 자동화 방법 및 장치

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US (2) US8815585B2 (enExample)
EP (2) EP3293256B1 (enExample)
JP (4) JP5991796B2 (enExample)
KR (2) KR20160005142A (enExample)
AU (1) AU2008272949B2 (enExample)
CA (1) CA2691793A1 (enExample)
DK (2) DK3293256T3 (enExample)
WO (1) WO2009006422A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
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KR101293300B1 (ko) * 2011-04-05 2013-08-09 (주)로고스바이오시스템스 세포의 분리시기를 판정하는 방법과, 이를 이용한 세포의 계대 배양 방법 및 계대 배양 장치
KR20140101393A (ko) * 2011-12-01 2014-08-19 더 뉴욕 스템 셀 파운데이션 유도 다능성 줄기 세포 또는 분화된 세포를 제조하기 위한 자동화 시스템
KR20190124608A (ko) * 2018-04-26 2019-11-05 (주)세포바이오 세포배양 자동화 장치 및 방법
US11136556B2 (en) 2011-12-01 2021-10-05 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells

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DK2173863T3 (en) 2019-01-21
AU2008272949B2 (en) 2014-05-15
JP5991796B2 (ja) 2016-09-14
JP2010532173A (ja) 2010-10-07
AU2008272949A1 (en) 2009-01-08
EP3293256A1 (en) 2018-03-14
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WO2009006422A1 (en) 2009-01-08
US8815585B2 (en) 2014-08-26
EP3293256B1 (en) 2019-05-22
JP2014110817A (ja) 2014-06-19
US20130316445A1 (en) 2013-11-28
CA2691793A1 (en) 2009-01-08
US20090029462A1 (en) 2009-01-29
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