JP5991796B2 - 胚性幹細胞培養のための自動化された方法および装置 - Google Patents

胚性幹細胞培養のための自動化された方法および装置 Download PDF

Info

Publication number
JP5991796B2
JP5991796B2 JP2010515226A JP2010515226A JP5991796B2 JP 5991796 B2 JP5991796 B2 JP 5991796B2 JP 2010515226 A JP2010515226 A JP 2010515226A JP 2010515226 A JP2010515226 A JP 2010515226A JP 5991796 B2 JP5991796 B2 JP 5991796B2
Authority
JP
Japan
Prior art keywords
cells
cell
pluripotent
medium
population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2010515226A
Other languages
English (en)
Japanese (ja)
Other versions
JP2010532173A5 (enExample
JP2010532173A (ja
Inventor
ナサニエル ベアーズリー，
ナサニエル ベアーズリー，
ベイト バーゲンダル，
ベイト バーゲンダル，
メーガン フィッツジェラルド，
メーガン フィッツジェラルド，
クリスティン ダイグ，
クリスティン ダイグ，
Original Assignee
セルラー ダイナミクス インターナショナル， インコーポレイテッド
セルラー ダイナミクス インターナショナル， インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by セルラー ダイナミクス インターナショナル， インコーポレイテッド, セルラー ダイナミクス インターナショナル， インコーポレイテッド filed Critical セルラー ダイナミクス インターナショナル， インコーポレイテッド
Publication of JP2010532173A publication Critical patent/JP2010532173A/ja
Publication of JP2010532173A5 publication Critical patent/JP2010532173A5/ja
Application granted granted Critical
Publication of JP5991796B2 publication Critical patent/JP5991796B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/07—Dosage or metering devices therefore
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Virology (AREA)
  • Transplantation (AREA)
JP2010515226A 2007-06-29 2008-06-30 胚性幹細胞培養のための自動化された方法および装置 Active JP5991796B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94701307P 2007-06-29 2007-06-29
US60/947,013 2007-06-29
PCT/US2008/068814 WO2009006422A1 (en) 2007-06-29 2008-06-30 Automated method and apparatus for embryonic stem cell culture

Related Child Applications (2)

Application Number Title Priority Date Filing Date
JP2014063625A Division JP2014110817A (ja) 2007-06-29 2014-03-26 胚性幹細胞培養のための自動化された方法および装置
JP2016125186A Division JP2016168056A (ja) 2007-06-29 2016-06-24 胚性幹細胞培養のための自動化された方法および装置

Publications (3)

Publication Number Publication Date
JP2010532173A JP2010532173A (ja) 2010-10-07
JP2010532173A5 JP2010532173A5 (enExample) 2012-07-26
JP5991796B2 true JP5991796B2 (ja) 2016-09-14

Family

ID=39672032

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2010515226A Active JP5991796B2 (ja) 2007-06-29 2008-06-30 胚性幹細胞培養のための自動化された方法および装置
JP2014063625A Pending JP2014110817A (ja) 2007-06-29 2014-03-26 胚性幹細胞培養のための自動化された方法および装置
JP2016125186A Pending JP2016168056A (ja) 2007-06-29 2016-06-24 胚性幹細胞培養のための自動化された方法および装置
JP2016217785A Pending JP2017023158A (ja) 2007-06-29 2016-11-08 胚性幹細胞培養のための自動化された方法および装置

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2014063625A Pending JP2014110817A (ja) 2007-06-29 2014-03-26 胚性幹細胞培養のための自動化された方法および装置
JP2016125186A Pending JP2016168056A (ja) 2007-06-29 2016-06-24 胚性幹細胞培養のための自動化された方法および装置
JP2016217785A Pending JP2017023158A (ja) 2007-06-29 2016-11-08 胚性幹細胞培養のための自動化された方法および装置

Country Status (8)

Country Link
US (2) US8815585B2 (enExample)
EP (2) EP3293256B1 (enExample)
JP (4) JP5991796B2 (enExample)
KR (2) KR20100059789A (enExample)
AU (1) AU2008272949B2 (enExample)
CA (1) CA2691793A1 (enExample)
DK (2) DK2173863T3 (enExample)
WO (1) WO2009006422A1 (enExample)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8476070B2 (en) 2005-08-29 2013-07-02 Technion Research & Development Foundation Limited Media for culturing stem cells
ES2704401T3 (es) 2006-08-02 2019-03-18 Technion Res & Dev Foundation Métodos de expansión de células madre embrionarias en un cultivo en suspensión
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
US9096832B2 (en) 2007-07-31 2015-08-04 Lifescan, Inc. Differentiation of human embryonic stem cells
CA2959401C (en) 2008-02-21 2021-12-07 Centocor Ortho Biotech Inc. Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
JP5951254B2 (ja) 2008-03-17 2016-07-13 ザ スクリプス リサーチ インスティテュート 人工多能性幹細胞を作製するための化学的手法と遺伝的手法の組み合わせ法
US8691569B2 (en) 2008-03-17 2014-04-08 Agency For Science, Technology And Research Microcarriers for stem cell culture
US8828720B2 (en) 2008-03-17 2014-09-09 Agency For Science, Technology And Research Microcarriers for stem cell culture
US9458431B2 (en) 2008-03-17 2016-10-04 Agency For Science, Technology And Research Microcarriers for stem cell culture
SG188918A1 (enExample) 2008-03-17 2013-04-30 Agency Science Tech & Res
KR101651661B1 (ko) 2008-06-30 2016-08-26 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
JP5688970B2 (ja) * 2008-07-07 2015-03-25 タカラバイオ株式会社 多能性幹細胞の製造方法
PL2366022T3 (pl) 2008-11-20 2016-11-30 Sposoby i kompozycje do przyłączania i hodowli komórek na podłożach planarnych
PL2366021T3 (pl) 2008-11-20 2017-12-29 Janssen Biotech, Inc. Hodowla pluripotencjalnych komórek macierzystych na mikronośnikach
JP6093110B2 (ja) 2008-12-17 2017-03-08 ザ スクリプス リサーチ インスティテュート 幹細胞の作製と維持
EP2398897B1 (en) 2009-02-20 2017-06-28 Cellular Dynamics International, Inc. Methods and compositions for the differentiation of stem cells
EP2408903B1 (en) * 2009-03-20 2014-08-06 Agency For Science, Technology And Research Culture of pluripotent and multipotent cells on microcarriers
KR20170118969A (ko) 2009-07-20 2017-10-25 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
US9008406B2 (en) * 2009-10-09 2015-04-14 Kawasaki Jukogyo Kabushiki Kaisha Method and apparatus for discriminating undifferentiated pluripotent stem cells, and automated culture method and system
US9005968B2 (en) * 2009-10-16 2015-04-14 The Scripps Research Institute Induction of pluripotent cells
JP5898086B2 (ja) * 2009-11-04 2016-04-06 セルラー ダイナミクス インターナショナル， インコーポレイテッド 化学物質を用いるエピソームリプログラミング
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
US8349609B2 (en) 2009-11-24 2013-01-08 University Of Connecticut Differentiation of human embryonic and induced pluripotent stem cells
AU2010333839C1 (en) 2009-12-23 2016-07-21 Janssen Biotech Inc. Differentiation of human embryonic stem cells
CN107189979B (zh) 2010-03-01 2021-05-04 詹森生物科技公司 纯化衍生自多能干细胞的细胞的方法
EP2553086B1 (en) 2010-03-31 2017-04-19 The Scripps Research Institute Reprogramming cells
EP2566951B1 (en) * 2010-05-05 2020-03-04 Terumo BCT, Inc. Method of reseeding adherent cells grown in a hollow fiber bioreactor system
US20130071927A1 (en) * 2010-05-05 2013-03-21 Sydney Ivf Limited Media and methods for cell culture
EP3399026B1 (en) 2010-06-14 2024-06-26 The Scripps Research Institute Reprogramming of cells to a new fate
US8977031B2 (en) 2010-06-25 2015-03-10 Kawasaki Jukogyo Kabushiki Kaisha Method and device for identifying multipotent stem cell colony, and method and device for automatically culturing multipotent stem cells
AU2011296381B2 (en) 2010-08-31 2016-03-31 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
WO2012032521A2 (en) 2010-09-07 2012-03-15 Technion Research & Development Foundation Ltd. Novel methods and culture media for culturing pluripotent stem cells
AU2011349446C1 (en) 2010-12-22 2016-01-21 Fate Therapauetics, Inc. Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs
KR101293300B1 (ko) * 2011-04-05 2013-08-09 (주)로고스바이오시스템스 세포의 분리시기를 판정하는 방법과, 이를 이용한 세포의 계대 배양 방법 및 계대 배양 장치
WO2013058403A1 (ja) * 2011-10-21 2013-04-25 国立大学法人京都大学 層流による多能性維持単一分散細胞培養法
US10428309B2 (en) 2011-12-01 2019-10-01 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells
AU2012345676B2 (en) * 2011-12-01 2018-10-04 New York Stem Cell Foundation, Inc. Automated system for producing induced pluripotent stem cells or differentiated cells
US9388386B2 (en) 2011-12-22 2016-07-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
CN102604894B (zh) 2012-02-29 2014-07-30 中国科学院广州生物医药与健康研究院 用于制备神经干细胞的培养基及其用途
WO2013184888A1 (en) 2012-06-08 2013-12-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells
WO2014105543A1 (en) 2012-12-31 2014-07-03 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
RU2684215C2 (ru) 2012-12-31 2019-04-04 Янссен Байотек, Инк. Способ получения панкреатических эндокринных клеток (варианты) и способ увеличения выхода бета-клеток
RU2687379C2 (ru) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
US9790465B2 (en) 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
WO2015006751A1 (en) 2013-07-12 2015-01-15 President And Fellows Of Harvard College Systems and methods for cell culture device interconnection and fluidic device interconnection
CA2920667C (en) 2013-08-12 2023-02-21 Invivosciences Inc. Automated system and method for culturing stem cells
SG11201606934SA (en) 2014-03-04 2016-09-29 Fate Therapeutics Inc Improved reprogramming methods and cell culture platforms
JP6588969B2 (ja) 2014-05-16 2019-10-09 ヤンセン バイオテツク，インコーポレーテツド 膵内分泌細胞内のｍａｆａ発現を強化するための小分子の使用
EP3183338B1 (en) 2014-08-19 2020-03-04 FUJIFILM Cellular Dynamics, Inc. Neural networks formed from cells derived from pluripotent stem cells
CN120924475A (zh) 2014-09-18 2025-11-11 北卡罗来纳大学 哺乳动物肺球体和肺球体细胞及其应用
SG11201703493SA (en) 2014-10-29 2017-05-30 Corning Inc Cell culture insert
EP3212760A1 (en) 2014-10-29 2017-09-06 Corning Incorporated Perfusion bioreactor platform
JP2017532971A (ja) 2014-10-29 2017-11-09 コーニング インコーポレイテッド 細胞培養集合体を生成するためのマイクロウェル設計および製造
EP3234109A4 (en) 2014-12-19 2018-06-27 Janssen Biotech, Inc. Suspension culturing of pluripotent stem cells
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
JP7420334B2 (ja) * 2015-06-25 2024-01-23 株式会社カネカ 液体注入方法
WO2017007278A1 (ko) * 2015-07-09 2017-01-12 사회복지법인 삼성생명공익재단 자동 세포 배양기 및 그 배양기의 동작 방법
US11286454B2 (en) 2015-08-31 2022-03-29 I Peace, Inc. Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells
AU2016331926B2 (en) 2015-10-01 2021-12-09 Berkeley Lights, Inc. Well-plate incubator
CA3001917A1 (en) 2015-10-16 2017-04-20 Fate Therapeutics, Inc. Platform for the induction & maintenance of ground state pluripotency
US11674952B2 (en) 2016-02-24 2023-06-13 The Rockefeller University Embryonic cell-based therapeutic candidate screening systems, models for Huntington's Disease and uses thereof
JP6943449B2 (ja) * 2016-03-21 2021-09-29 グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー 撹拌タンクバイオリアクタを用いた多能性幹細胞の増殖および継代
JP6705911B2 (ja) 2016-04-14 2020-06-03 ヤンセン バイオテツク，インコーポレーテツド 多能性幹細胞の中腸内胚葉細胞への分化
JP2018000130A (ja) * 2016-07-05 2018-01-11 株式会社Ihi 細胞培養装置
KR20240145081A (ko) 2016-07-19 2024-10-04 아셀라타 리미티드 현탁 내 만능줄기세포를 배양하기 위한 배양 배지
DE102016114043B3 (de) 2016-07-29 2017-08-10 Technische Universität Dresden Vorrichtung zur Isolierung von Stammzellen aus fötalen Geweben
EP3504315A4 (en) 2016-08-27 2020-04-15 3D Biotek, LLC BIOREACTOR
ES2956547T3 (es) 2016-11-10 2023-12-22 Becton Dickinson Co Sistema de línea de tiempo para supervisar un protocolo de medio de cultivo
CN110366591A (zh) 2016-12-01 2019-10-22 伯克利之光生命科技公司 井孔板培养器
US20200239823A1 (en) * 2017-02-27 2020-07-30 Koji Tanabe Cell processing system and cell processing device
US20190382706A1 (en) * 2017-02-27 2019-12-19 Koji Tanabe Somatic cell production system
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
JP7245222B2 (ja) 2017-07-14 2023-03-23 コーニング インコーポレイテッド 手動又は自動で培地を交換するための３ｄ細胞培養容器
CN111094536B (zh) 2017-07-14 2024-03-29 康宁股份有限公司 用于3d培养的细胞培养容器及培养3d细胞的方法
JP7197557B2 (ja) 2017-07-14 2022-12-27 コーニング インコーポレイテッド 多孔性支持体を有する細胞培養槽
KR102220125B1 (ko) * 2018-04-26 2021-02-25 (주)세포바이오 세포배양 자동화 장치 및 방법
JP7171695B2 (ja) 2018-07-13 2022-11-15 コーニング インコーポレイテッド 液体培地送達面を含む側壁を有するマイクロキャビティ皿
JP7171696B2 (ja) 2018-07-13 2022-11-15 コーニング インコーポレイテッド 相互接続されたウェルを有するマイクロプレートを備えた流体デバイス
PL3649229T3 (pl) 2018-07-13 2021-12-06 Corning Incorporated Naczynia do hodowli komórkowych ze stabilizującymi urządzeniami
US20230035127A1 (en) * 2021-07-21 2023-02-02 University Of Maryland, College Park Culture and differentiation of pluripotent stem cells

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4352883A (en) 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
US4284412A (en) 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
JPS58155087A (ja) * 1982-03-12 1983-09-14 Olympus Optical Co Ltd 細胞の自動培養装置
US4498766A (en) 1982-03-25 1985-02-12 Becton, Dickinson And Company Light beam focal spot elongation in flow cytometry devices
US4661913A (en) 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4767206A (en) 1984-12-24 1988-08-30 Flow Cytometry Standards Corporation Calibration method for flow cytometry using fluorescent microbeads and synthesis thereof
US4857451A (en) 1984-12-24 1989-08-15 Flow Cytometry Standards Corporation Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor
US4774189A (en) 1984-12-24 1988-09-27 Flow Cytometry Standards Corp. Fluorescent calibration microbeads simulating stained cells
US4989977A (en) 1985-07-29 1991-02-05 Becton, Dickinson And Company Flow cytometry apparatus with improved light beam adjustment
US4714682A (en) 1985-12-11 1987-12-22 Flow Cytometry Standards Corporation Fluorescent calibration microbeads simulating stained cells
US5160974A (en) 1990-06-25 1992-11-03 Flow Science, Inc. Closed sample cell for use in flow cytometry
US5478722A (en) 1991-02-17 1995-12-26 The Curators Of The University Of Missouri Preserved cell preparations for flow cytometry and immunology
JP3246664B2 (ja) 1993-01-29 2002-01-15 ニュー ブルンズウイック サイエンティフィック カンパニー インコーポレイテッド 足場および懸濁細胞の培養方法並びにその装置
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6325114B1 (en) 2000-02-01 2001-12-04 Incyte Genomics, Inc. Pipetting station apparatus
US8492140B2 (en) * 2002-04-08 2013-07-23 Octane Biotech Inc. Automated tissue engineering system
DE102004043256B4 (de) * 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
BRPI0514778B1 (pt) 2004-09-08 2018-07-10 Wisconsin Alumni Research Foundation Meio para cultivar células tronco embrionárias
WO2006084040A2 (en) 2005-02-04 2006-08-10 Massachusetts Institute Of Technology Engineering vascularized muscle tissue
US20060199265A1 (en) 2005-03-02 2006-09-07 Wolf Michael F Seeding implantable medical devices with cells

Also Published As

Publication number Publication date
JP2014110817A (ja) 2014-06-19
US20130316445A1 (en) 2013-11-28
DK3293256T3 (da) 2019-08-12
KR20160005142A (ko) 2016-01-13
EP3293256B1 (en) 2019-05-22
WO2009006422A1 (en) 2009-01-08
JP2017023158A (ja) 2017-02-02
JP2016168056A (ja) 2016-09-23
KR20100059789A (ko) 2010-06-04
AU2008272949B2 (en) 2014-05-15
US20090029462A1 (en) 2009-01-29
CA2691793A1 (en) 2009-01-08
EP3293256A1 (en) 2018-03-14
EP2173863A1 (en) 2010-04-14
AU2008272949A1 (en) 2009-01-08
JP2010532173A (ja) 2010-10-07
DK2173863T3 (en) 2019-01-21
US8815585B2 (en) 2014-08-26
EP2173863B1 (en) 2018-10-10

Similar Documents

Publication Publication Date Title
JP2017023158A (ja) 胚性幹細胞培養のための自動化された方法および装置
JP6849719B2 (ja) オートメーション化された細胞培養システム及び方法
JP7123412B2 (ja) オルガノイドを培養するための方法
CN101611139B (zh) 利用微载体的产后来源的细胞的体外扩增
JP7510247B2 (ja) 細胞凝集塊を解離させる方法
EP2398897B1 (en) Methods and compositions for the differentiation of stem cells
US20100093083A1 (en) Large scale production of stem cells
CN107267442A (zh) 微载体上的多能干细胞培养
Reid [12] Cloning
JP7804327B2 (ja) バイオリアクターにおける懸濁状態での幹細胞の増殖方法
AU2014201955B2 (en) Automated method and apparatus for embryonic stem cell culture
US20250122474A1 (en) Production method for natural killer cells
JP7092309B2 (ja) 単一細胞ヒト多能性幹細胞の継代及び採取用配合物
CA2675313A1 (en) Novel mesenchymal progenitor cells derived from human blastocyst-derived stem cells
WO2025062293A1 (en) Methods and materials for scalable 3d cellular redifferentiation
WO2025146565A1 (en) Scalable generation of human induced pluripotent stem cell derived cardiac organoids
Jing et al. Stem cell bioprocessing for regenerative medicine
HK40119737A (en) Production method for natural killer cells
CN115052966A (zh) 三维细胞培养

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110630

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20110630

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120530

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130611

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130808

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20131227

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140326

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20140326

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20140825

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141224

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20150203

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20150313

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20160422

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20160524

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160624

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160816

R150 Certificate of patent or registration of utility model

Ref document number: 5991796

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: R3D03

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: R3D04

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250