JP5991796B2 - 胚性幹細胞培養のための自動化された方法および装置 - Google Patents

胚性幹細胞培養のための自動化された方法および装置 Download PDF

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JP5991796B2
JP5991796B2 JP2010515226A JP2010515226A JP5991796B2 JP 5991796 B2 JP5991796 B2 JP 5991796B2 JP 2010515226 A JP2010515226 A JP 2010515226A JP 2010515226 A JP2010515226 A JP 2010515226A JP 5991796 B2 JP5991796 B2 JP 5991796B2
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JP2010532173A (ja
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ナサニエル ベアーズリー，
ナサニエル ベアーズリー，
ベイト バーゲンダル，
ベイト バーゲンダル，
メーガン フィッツジェラルド，
メーガン フィッツジェラルド，
クリスティン ダイグ，
クリスティン ダイグ，
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セルラー ダイナミクス インターナショナル， インコーポレイテッド
セルラー ダイナミクス インターナショナル， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
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    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • C—CHEMISTRY; METALLURGY
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/07—Dosage or metering devices therefore
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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JP2010515226A 2007-06-29 2008-06-30 胚性幹細胞培養のための自動化された方法および装置 Active JP5991796B2 (ja)

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US94701307P 2007-06-29 2007-06-29
US60/947,013 2007-06-29
PCT/US2008/068814 WO2009006422A1 (en) 2007-06-29 2008-06-30 Automated method and apparatus for embryonic stem cell culture

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JP2014063625A Division JP2014110817A (ja) 2007-06-29 2014-03-26 胚性幹細胞培養のための自動化された方法および装置
JP2016125186A Division JP2016168056A (ja) 2007-06-29 2016-06-24 胚性幹細胞培養のための自動化された方法および装置

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JP2010532173A JP2010532173A (ja) 2010-10-07
JP2010532173A5 JP2010532173A5 (enExample) 2012-07-26
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JP2016125186A Pending JP2016168056A (ja) 2007-06-29 2016-06-24 胚性幹細胞培養のための自動化された方法および装置
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JP2016217785A Pending JP2017023158A (ja) 2007-06-29 2016-11-08 胚性幹細胞培養のための自動化された方法および装置

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US (2) US8815585B2 (enExample)
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JP (4) JP5991796B2 (enExample)
KR (2) KR20100059789A (enExample)
AU (1) AU2008272949B2 (enExample)
CA (1) CA2691793A1 (enExample)
DK (2) DK2173863T3 (enExample)
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US20090029462A1 (en) 2009-01-29
JP2010532173A (ja) 2010-10-07
JP2017023158A (ja) 2017-02-02
US8815585B2 (en) 2014-08-26
CA2691793A1 (en) 2009-01-08
WO2009006422A1 (en) 2009-01-08
JP2014110817A (ja) 2014-06-19
US20130316445A1 (en) 2013-11-28
EP2173863A1 (en) 2010-04-14
KR20160005142A (ko) 2016-01-13
DK3293256T3 (da) 2019-08-12
EP3293256B1 (en) 2019-05-22
JP2016168056A (ja) 2016-09-23
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AU2008272949A1 (en) 2009-01-08
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