DK3293256T3 - Automatiseret fremgangsmåde og apparatur til dyrkning af embryonale stamceller - Google Patents

Automatiseret fremgangsmåde og apparatur til dyrkning af embryonale stamceller

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Publication number
DK3293256T3
DK3293256T3 DK17192886.4T DK17192886T DK3293256T3 DK 3293256 T3 DK3293256 T3 DK 3293256T3 DK 17192886 T DK17192886 T DK 17192886T DK 3293256 T3 DK3293256 T3 DK 3293256T3
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Denmark
Prior art keywords
stem cells
embryonic stem
automated method
culturing embryonic
culturing
Prior art date
Application number
DK17192886.4T
Other languages
Danish (da)
English (en)
Inventor
Nathaniel Beardsley
Veit Bergendahl
Megan Fitzgerald
Christine Daigh
Original Assignee
Fujifilm Cellular Dynamics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by Fujifilm Cellular Dynamics Inc filed Critical Fujifilm Cellular Dynamics Inc
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Publication of DK3293256T3 publication Critical patent/DK3293256T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/07—Dosage or metering devices therefore
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Virology (AREA)
  • Transplantation (AREA)
DK17192886.4T 2007-06-29 2008-06-30 Automatiseret fremgangsmåde og apparatur til dyrkning af embryonale stamceller DK3293256T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94701307P 2007-06-29 2007-06-29
EP08781187.3A EP2173863B1 (en) 2007-06-29 2008-06-30 Automated method and apparatus for embryonic stem cell culture

Publications (1)

Publication Number Publication Date
DK3293256T3 true DK3293256T3 (da) 2019-08-12

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
DK17192886.4T DK3293256T3 (da) 2007-06-29 2008-06-30 Automatiseret fremgangsmåde og apparatur til dyrkning af embryonale stamceller
DK08781187.3T DK2173863T3 (en) 2007-06-29 2008-06-30 Automated method and apparatus for embryonic stem cell culture

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK08781187.3T DK2173863T3 (en) 2007-06-29 2008-06-30 Automated method and apparatus for embryonic stem cell culture

Country Status (8)

Country Link
US (2) US8815585B2 (enExample)
EP (2) EP3293256B1 (enExample)
JP (4) JP5991796B2 (enExample)
KR (2) KR20160005142A (enExample)
AU (1) AU2008272949B2 (enExample)
CA (1) CA2691793A1 (enExample)
DK (2) DK3293256T3 (enExample)
WO (1) WO2009006422A1 (enExample)

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Also Published As

Publication number Publication date
JP2016168056A (ja) 2016-09-23
EP2173863B1 (en) 2018-10-10
EP2173863A1 (en) 2010-04-14
KR20160005142A (ko) 2016-01-13
KR20100059789A (ko) 2010-06-04
DK2173863T3 (en) 2019-01-21
AU2008272949B2 (en) 2014-05-15
JP5991796B2 (ja) 2016-09-14
JP2010532173A (ja) 2010-10-07
AU2008272949A1 (en) 2009-01-08
EP3293256A1 (en) 2018-03-14
WO2009006422A1 (en) 2009-01-08
US8815585B2 (en) 2014-08-26
EP3293256B1 (en) 2019-05-22
JP2014110817A (ja) 2014-06-19
US20130316445A1 (en) 2013-11-28
CA2691793A1 (en) 2009-01-08
US20090029462A1 (en) 2009-01-29
JP2017023158A (ja) 2017-02-02

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