KR20030004305A - 전사 후 유전자 사일런싱(ptgs)의 억제제와 함께발현시킴으로써 이식 유전자의 발현을 증가시키는 방법 - Google Patents

전사 후 유전자 사일런싱(ptgs)의 억제제와 함께발현시킴으로써 이식 유전자의 발현을 증가시키는 방법 Download PDF

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Publication number
KR20030004305A
KR20030004305A KR1020027006548A KR20027006548A KR20030004305A KR 20030004305 A KR20030004305 A KR 20030004305A KR 1020027006548 A KR1020027006548 A KR 1020027006548A KR 20027006548 A KR20027006548 A KR 20027006548A KR 20030004305 A KR20030004305 A KR 20030004305A
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South Korea
Prior art keywords
protein
gfp
nucleic acid
ptgs
inhibitor
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Ceased
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KR1020027006548A
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English (en)
Korean (ko)
Inventor
바울콤베데이비드찰스
보인네트올리비어
함밀톤앤드류존
Original Assignee
플랜트 바이오사이언스 리미티드
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Filing date
Publication date
Priority claimed from GBGB9927609.9A external-priority patent/GB9927609D0/en
Priority claimed from GB0022960A external-priority patent/GB0022960D0/en
Priority claimed from GB0023668A external-priority patent/GB0023668D0/en
Application filed by 플랜트 바이오사이언스 리미티드 filed Critical 플랜트 바이오사이언스 리미티드
Publication of KR20030004305A publication Critical patent/KR20030004305A/ko
Ceased legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203Virus mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
KR1020027006548A 1999-11-22 2000-11-22 전사 후 유전자 사일런싱(ptgs)의 억제제와 함께발현시킴으로써 이식 유전자의 발현을 증가시키는 방법 Ceased KR20030004305A (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GBGB9927609.9A GB9927609D0 (en) 1999-11-22 1999-11-22 Enhanced expression
GB9927609.9 1999-11-22
GB0022960.9 2000-09-19
GB0022960A GB0022960D0 (en) 2000-09-19 2000-09-19 Enhanced expression
GB0023668A GB0023668D0 (en) 2000-09-27 2000-09-27 Enhanced expression
GB0023668.7 2000-09-27

Publications (1)

Publication Number Publication Date
KR20030004305A true KR20030004305A (ko) 2003-01-14

Family

ID=27255894

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020027006548A Ceased KR20030004305A (ko) 1999-11-22 2000-11-22 전사 후 유전자 사일런싱(ptgs)의 억제제와 함께발현시킴으로써 이식 유전자의 발현을 증가시키는 방법

Country Status (12)

Country Link
US (2) US7217854B1 (enExample)
EP (1) EP1232274B1 (enExample)
JP (1) JP4884628B2 (enExample)
KR (1) KR20030004305A (enExample)
AT (1) ATE460490T1 (enExample)
AU (1) AU782788B2 (enExample)
CA (1) CA2390152C (enExample)
DE (1) DE60043992D1 (enExample)
IL (1) IL149388A0 (enExample)
MX (1) MXPA02005089A (enExample)
NZ (1) NZ518947A (enExample)
WO (1) WO2001038512A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101334846B1 (ko) * 2011-07-20 2013-11-29 대한민국 벼줄무늬잎마름병 바이러스 유래 ns3유전자의 신규 용도

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DE60029986T2 (de) * 1999-11-12 2007-09-06 University Of South Carolina Kontrolle der post-transkriptionellen Genabschaltung (gene silencing) in Pflanzen
US6972349B1 (en) 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
GB0101513D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst 'Gene silencing suppressor'
GB0101505D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst Protection against gene silencing
WO2004009779A2 (en) 2002-07-19 2004-01-29 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
US7692063B2 (en) 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
US7683238B2 (en) 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
ES2531125T3 (es) 2003-02-03 2015-03-10 Ibio Inc Sistema para la expresión de genes en plantas
CA2540668C (en) * 2003-10-01 2010-12-21 Japan Science And Technology Agency Dna fragment, method for producting transformant for producing transformant for protein production and utilization thereof
GB0327919D0 (en) * 2003-12-02 2004-01-07 Plant Bioscience Ltd Enhanced expression
EP1769068B1 (en) * 2004-02-20 2014-12-31 iBio, Inc. Systems and methods for clonal expression in plants
WO2005103253A1 (en) 2004-04-22 2005-11-03 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
US7807849B2 (en) 2004-04-22 2010-10-05 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
EP1616959A1 (en) * 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants
FR2877014B1 (fr) * 2004-10-21 2009-07-10 Inst Rech Pour Le Dev I R D Et Systemes d'expression de proteines recombinantes et leurs applications
FR2880357B1 (fr) * 2005-01-05 2010-12-10 Inst Rech Developpement Ird Vecteurs viraux pour la surexpression ou pour l'extinction de genes d'interet chez les plantes et leurs applications
US8222488B2 (en) 2006-02-03 2012-07-17 Texas A&M University System Enhancing expression of value-added genes by transgenic expression of tombusvirus-based P19 gene mutants
RU2009111266A (ru) 2006-08-29 2010-10-10 Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU) Синтез жирных кислот
EP1897953A1 (en) 2006-09-06 2008-03-12 Icon Genetics GmbH Potexvirus-derived replicon
WO2008036424A2 (en) * 2006-09-22 2008-03-27 Regents Of The University Of California Chemically inducible cucumber mosaic virus protein expression system
EP2118274A4 (en) * 2007-01-29 2010-09-01 Univ Ohio State Res Found GENE EXPRESSION SYSTEM IN PLANTS WITH A VIRUS-BASED EXPRESSION VECTOR
US20080317881A1 (en) * 2007-05-09 2008-12-25 Devgen N.V. Method for sustainable transgene transcription
WO2010025285A1 (en) * 2008-08-27 2010-03-04 Arizona Board Of Regents For And On Behalf Of Arizona State University A dna replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants
AU2009317860B2 (en) 2008-11-18 2014-03-27 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
AU2013204254B8 (en) * 2008-11-18 2015-10-08 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
WO2010099462A2 (en) * 2009-02-26 2010-09-02 Baylor University Highly efficient suppressor-dependent protein expression in plants with a viral vector
US20110197312A1 (en) * 2010-02-07 2011-08-11 University Of Kuwait Indigenous Viral Satellite RNA Associate with Cucumber Mosaic Virus used as a Biological Control Agent against Tomato Diseases caused by Virus Strains, Viroids, and Fungi
FR2960550A1 (fr) * 2010-05-26 2011-12-02 Inst Rech R Pour Le Dev Ird Moyens pour la production transitoire dans les plantes de proteines recombinantes utilisables notamment en prophylaxie et en therapeutique
FI20100248A0 (fi) 2010-06-11 2010-06-11 Kristiina Maekinen Menetelmä proteiinien tuottamiseksi
WO2012098111A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
BR112013018130A2 (pt) 2011-01-17 2017-06-27 Philip Morris Products Sa "método para produzir um polipeptídeo heterólogo em nicotiana tabacum, composição compreendendo um polipeptídeo por tal método e sistema para aplicação do mesmo"
CA2860432C (en) * 2011-12-27 2024-06-04 Commonwealth Scientific And Industrial Research Organisation Simultaneous gene silencing and suppressing gene silencing in the same cell
US8816111B2 (en) 2012-06-15 2014-08-26 Commonwealth Scientific And Industrial Research Organisation Lipid comprising polyunsaturated fatty acids
WO2015196250A1 (en) 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
AU2014366839B2 (en) 2013-12-18 2021-07-01 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
WO2015168733A1 (en) * 2014-05-05 2015-11-12 Queensland University Of Technology Decoy molecules
US11649465B2 (en) 2017-09-11 2023-05-16 R.J. Reynolds Tobacco Company Methods and compositions for increasing expression of genes of interest in a plant by co-expression with p21
MX2021005555A (es) * 2018-11-13 2021-09-10 Univ Maryland Vectores vegetales, composiciones y usos relacionados con los mismos.
CN115851820B (zh) * 2022-12-19 2024-06-18 内蒙古农业大学 一株烟草脉斑驳病毒的应用
CN119410670A (zh) * 2025-01-06 2025-02-11 中国热带农业科学院三亚研究院 斯里兰卡木薯花叶病毒转录激活因子ac2及其应用

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US5939541A (en) * 1997-03-28 1999-08-17 University Of South Carolina Method for enhancing expression of a foreign or endogenous gene product in plants
US6972349B1 (en) * 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
DE60029986T2 (de) * 1999-11-12 2007-09-06 University Of South Carolina Kontrolle der post-transkriptionellen Genabschaltung (gene silencing) in Pflanzen

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101334846B1 (ko) * 2011-07-20 2013-11-29 대한민국 벼줄무늬잎마름병 바이러스 유래 ns3유전자의 신규 용도

Also Published As

Publication number Publication date
IL149388A0 (en) 2002-11-10
EP1232274A2 (en) 2002-08-21
CA2390152A1 (en) 2001-05-31
MXPA02005089A (es) 2003-01-28
WO2001038512A3 (en) 2002-03-28
US20070292862A1 (en) 2007-12-20
AU782788B2 (en) 2005-08-25
DE60043992D1 (de) 2010-04-22
WO2001038512A2 (en) 2001-05-31
US7217854B1 (en) 2007-05-15
AU1533401A (en) 2001-06-04
CA2390152C (en) 2012-08-28
EP1232274B1 (en) 2010-03-10
US8871997B2 (en) 2014-10-28
JP4884628B2 (ja) 2012-02-29
ATE460490T1 (de) 2010-03-15
JP2003514585A (ja) 2003-04-22
NZ518947A (en) 2004-05-28

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