MXPA02005089A - Expresion de transgen mejorada por la coexpresion con un supresor del silenciamiento del gen post-transcripcional (ptgs). - Google Patents
Expresion de transgen mejorada por la coexpresion con un supresor del silenciamiento del gen post-transcripcional (ptgs).Info
- Publication number
- MXPA02005089A MXPA02005089A MXPA02005089A MXPA02005089A MXPA02005089A MX PA02005089 A MXPA02005089 A MX PA02005089A MX PA02005089 A MXPA02005089 A MX PA02005089A MX PA02005089 A MXPA02005089 A MX PA02005089A MX PA02005089 A MXPA02005089 A MX PA02005089A
- Authority
- MX
- Mexico
- Prior art keywords
- protein
- plant
- gfp
- suppressor
- nucleic acid
- Prior art date
Links
- 230000014509 gene expression Effects 0.000 title claims abstract description 136
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 272
- 241000196324 Embryophyta Species 0.000 claims abstract description 255
- 230000032361 posttranscriptional gene silencing Effects 0.000 claims abstract description 187
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 185
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 104
- 238000000034 method Methods 0.000 claims abstract description 102
- 239000013598 vector Substances 0.000 claims abstract description 98
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 89
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 89
- 241000709992 Potato virus X Species 0.000 claims abstract description 67
- 108700019146 Transgenes Proteins 0.000 claims abstract description 64
- 101710142246 External core antigen Proteins 0.000 claims abstract description 63
- 239000002773 nucleotide Substances 0.000 claims abstract description 53
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 53
- 230000003612 virological effect Effects 0.000 claims abstract description 39
- 241000710145 Tomato bushy stunt virus Species 0.000 claims abstract description 26
- 241000702449 African cassava mosaic virus Species 0.000 claims abstract description 19
- 230000003362 replicative effect Effects 0.000 claims abstract description 14
- 102100029008 Putative HTLV-1-related endogenous sequence Human genes 0.000 claims abstract 4
- 230000030279 gene silencing Effects 0.000 claims description 159
- 241000700605 Viruses Species 0.000 claims description 136
- 210000001519 tissue Anatomy 0.000 claims description 114
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 108
- 210000004027 cell Anatomy 0.000 claims description 95
- 230000009885 systemic effect Effects 0.000 claims description 87
- 238000010276 construction Methods 0.000 claims description 56
- 210000003462 vein Anatomy 0.000 claims description 40
- 108020004999 messenger RNA Proteins 0.000 claims description 36
- 108020004414 DNA Proteins 0.000 claims description 32
- 239000000463 material Substances 0.000 claims description 22
- 230000010076 replication Effects 0.000 claims description 20
- 230000001976 improved effect Effects 0.000 claims description 16
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 13
- 238000013518 transcription Methods 0.000 claims description 11
- 230000035897 transcription Effects 0.000 claims description 11
- 230000015556 catabolic process Effects 0.000 claims description 10
- 238000006731 degradation reaction Methods 0.000 claims description 10
- 239000013604 expression vector Substances 0.000 claims description 10
- 240000007594 Oryza sativa Species 0.000 claims description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims description 9
- 230000001939 inductive effect Effects 0.000 claims description 9
- 235000009566 rice Nutrition 0.000 claims description 9
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 8
- 241001465754 Metazoa Species 0.000 claims description 8
- 244000061456 Solanum tuberosum Species 0.000 claims description 7
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 238000012546 transfer Methods 0.000 claims description 7
- 239000013603 viral vector Substances 0.000 claims description 7
- 230000006872 improvement Effects 0.000 claims description 6
- 230000001124 posttranscriptional effect Effects 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 206010003694 Atrophy Diseases 0.000 claims description 5
- 230000037444 atrophy Effects 0.000 claims description 5
- 230000005014 ectopic expression Effects 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 4
- 210000000805 cytoplasm Anatomy 0.000 claims description 4
- 238000003780 insertion Methods 0.000 claims description 4
- 210000001082 somatic cell Anatomy 0.000 claims description 4
- 108060001084 Luciferase Proteins 0.000 claims description 3
- 239000005089 Luciferase Substances 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 239000004009 herbicide Substances 0.000 claims description 3
- 230000006798 recombination Effects 0.000 claims description 3
- 238000005215 recombination Methods 0.000 claims description 3
- 241000238631 Hexapoda Species 0.000 claims description 2
- 230000006652 catabolic pathway Effects 0.000 claims description 2
- 230000037353 metabolic pathway Effects 0.000 claims description 2
- 241000227653 Lycopersicon Species 0.000 claims 3
- 101710190786 PI protein Proteins 0.000 claims 3
- 108091061960 Naked DNA Proteins 0.000 claims 1
- 101800001151 Protein p25 Proteins 0.000 claims 1
- 230000001172 regenerating effect Effects 0.000 claims 1
- 241001429314 Rice yellow mottle virus Species 0.000 abstract description 15
- 101000846901 Drosophila melanogaster Fat-body protein 1 Proteins 0.000 abstract 1
- 230000010474 transient expression Effects 0.000 abstract 1
- 239000005090 green fluorescent protein Substances 0.000 description 212
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 189
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 184
- 235000018102 proteins Nutrition 0.000 description 118
- 101710132601 Capsid protein Proteins 0.000 description 94
- 101710094648 Coat protein Proteins 0.000 description 94
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 94
- 101710125418 Major capsid protein Proteins 0.000 description 94
- 101710141454 Nucleoprotein Proteins 0.000 description 94
- 101710083689 Probable capsid protein Proteins 0.000 description 94
- 241000589158 Agrobacterium Species 0.000 description 55
- 102400000125 Cyclin-dependent kinase 5 activator 1, p25 Human genes 0.000 description 54
- 230000000694 effects Effects 0.000 description 54
- 230000001629 suppression Effects 0.000 description 45
- 230000009261 transgenic effect Effects 0.000 description 42
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 41
- 238000004458 analytical method Methods 0.000 description 39
- 108700026244 Open Reading Frames Proteins 0.000 description 38
- 238000002474 experimental method Methods 0.000 description 35
- 241000207746 Nicotiana benthamiana Species 0.000 description 33
- 238000001764 infiltration Methods 0.000 description 28
- 230000002123 temporal effect Effects 0.000 description 24
- 239000002299 complementary DNA Substances 0.000 description 23
- 230000037430 deletion Effects 0.000 description 23
- 238000012217 deletion Methods 0.000 description 23
- 230000008595 infiltration Effects 0.000 description 22
- 239000013612 plasmid Substances 0.000 description 22
- 241000894007 species Species 0.000 description 22
- 230000001419 dependent effect Effects 0.000 description 21
- 238000012226 gene silencing method Methods 0.000 description 21
- 238000005286 illumination Methods 0.000 description 21
- 108091093088 Amplicon Proteins 0.000 description 20
- 101000737786 Daucus carota Calcium-dependent protein kinase Proteins 0.000 description 20
- 101000737787 Glycine max Calcium-dependent protein kinase SK5 Proteins 0.000 description 20
- 208000015181 infectious disease Diseases 0.000 description 20
- 239000012634 fragment Substances 0.000 description 19
- 230000033001 locomotion Effects 0.000 description 18
- 238000009825 accumulation Methods 0.000 description 17
- 230000035772 mutation Effects 0.000 description 17
- 239000000523 sample Substances 0.000 description 17
- 208000024891 symptom Diseases 0.000 description 17
- 238000012360 testing method Methods 0.000 description 16
- 241000724252 Cucumber mosaic virus Species 0.000 description 15
- 230000007246 mechanism Effects 0.000 description 15
- 238000004519 manufacturing process Methods 0.000 description 14
- 230000001404 mediated effect Effects 0.000 description 14
- 241000723873 Tobacco mosaic virus Species 0.000 description 13
- 108020000999 Viral RNA Proteins 0.000 description 13
- 108090000765 processed proteins & peptides Proteins 0.000 description 13
- 241000723655 Cowpea mosaic virus Species 0.000 description 12
- 238000003556 assay Methods 0.000 description 12
- 238000011081 inoculation Methods 0.000 description 12
- 102000004196 processed proteins & peptides Human genes 0.000 description 12
- 108020004418 ribosomal RNA Proteins 0.000 description 12
- 238000001262 western blot Methods 0.000 description 12
- 241000709996 Narcissus mosaic virus Species 0.000 description 11
- 239000013614 RNA sample Substances 0.000 description 11
- 230000009466 transformation Effects 0.000 description 11
- 241000963701 Nandina virus X Species 0.000 description 10
- 241000208125 Nicotiana Species 0.000 description 10
- 241000963699 Viola mosaic virus Species 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 10
- 229920001184 polypeptide Polymers 0.000 description 10
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- 239000006185 dispersion Substances 0.000 description 9
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 9
- 229960005542 ethidium bromide Drugs 0.000 description 9
- 230000004927 fusion Effects 0.000 description 9
- 230000009467 reduction Effects 0.000 description 9
- 230000002829 reductive effect Effects 0.000 description 9
- 230000002459 sustained effect Effects 0.000 description 9
- 238000003786 synthesis reaction Methods 0.000 description 9
- 241000710017 Foxtail mosaic virus Species 0.000 description 8
- 108091005804 Peptidases Proteins 0.000 description 8
- 241000710007 Potexvirus Species 0.000 description 8
- 239000000499 gel Substances 0.000 description 8
- 230000001105 regulatory effect Effects 0.000 description 8
- 229920006395 saturated elastomer Polymers 0.000 description 8
- 101000609447 Beet necrotic yellow vein virus (isolate Japan/S) Protein P25 Proteins 0.000 description 7
- 125000000174 L-prolyl group Chemical group [H]N1C([H])([H])C([H])([H])C([H])([H])[C@@]1([H])C(*)=O 0.000 description 7
- 108700008625 Reporter Genes Proteins 0.000 description 7
- 239000000306 component Substances 0.000 description 7
- 230000006870 function Effects 0.000 description 7
- 238000011068 loading method Methods 0.000 description 7
- 238000012809 post-inoculation Methods 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 238000011002 quantification Methods 0.000 description 7
- 238000010186 staining Methods 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000035195 Peptidases Human genes 0.000 description 6
- OJOBTAOGJIWAGB-UHFFFAOYSA-N acetosyringone Chemical compound COC1=CC(C(C)=O)=CC(OC)=C1O OJOBTAOGJIWAGB-UHFFFAOYSA-N 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 238000002224 dissection Methods 0.000 description 6
- 230000001965 increasing effect Effects 0.000 description 6
- 239000000411 inducer Substances 0.000 description 6
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 6
- 238000012423 maintenance Methods 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 235000019833 protease Nutrition 0.000 description 6
- 241000724328 Alfalfa mosaic virus Species 0.000 description 5
- 101710118046 RNA-directed RNA polymerase Proteins 0.000 description 5
- 240000003768 Solanum lycopersicum Species 0.000 description 5
- 230000009471 action Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 230000002950 deficient Effects 0.000 description 5
- 238000004520 electroporation Methods 0.000 description 5
- 230000037433 frameshift Effects 0.000 description 5
- 230000002458 infectious effect Effects 0.000 description 5
- 230000000977 initiatory effect Effects 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 230000036961 partial effect Effects 0.000 description 5
- 230000007918 pathogenicity Effects 0.000 description 5
- 230000037361 pathway Effects 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- 238000006467 substitution reaction Methods 0.000 description 5
- 230000014616 translation Effects 0.000 description 5
- 230000009385 viral infection Effects 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 4
- 101000933461 Escherichia coli (strain K12) Beta-glucuronidase Proteins 0.000 description 4
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- 241000702463 Geminiviridae Species 0.000 description 4
- 238000000636 Northern blotting Methods 0.000 description 4
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 4
- 108091027544 Subgenomic mRNA Proteins 0.000 description 4
- 230000004913 activation Effects 0.000 description 4
- 229940024606 amino acid Drugs 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- 230000000692 anti-sense effect Effects 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 231100000221 frame shift mutation induction Toxicity 0.000 description 4
- 230000009368 gene silencing by RNA Effects 0.000 description 4
- 238000001415 gene therapy Methods 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- 230000002792 vascular Effects 0.000 description 4
- 101710097567 12 kDa protein Proteins 0.000 description 3
- 108020005544 Antisense RNA Proteins 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 241000723607 Comovirus Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 101150066002 GFP gene Proteins 0.000 description 3
- 241000124008 Mammalia Species 0.000 description 3
- 101710164418 Movement protein TGB2 Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 101710189610 Protein 2b Proteins 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 108091081024 Start codon Proteins 0.000 description 3
- 101710149950 Suppressor of silencing 2b Proteins 0.000 description 3
- 101001086866 Sus scrofa Pulmonary surfactant-associated protein B Proteins 0.000 description 3
- 241000710141 Tombusvirus Species 0.000 description 3
- 108700005077 Viral Genes Proteins 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 230000007123 defense Effects 0.000 description 3
- 230000005860 defense response to virus Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 239000003623 enhancer Substances 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000017074 necrotic cell death Effects 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 230000011664 signaling Effects 0.000 description 3
- 238000005063 solubilization Methods 0.000 description 3
- 230000007928 solubilization Effects 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 230000028604 virus induced gene silencing Effects 0.000 description 3
- 241000219194 Arabidopsis Species 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 2
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 241000723638 Nepovirus Species 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- 206010034133 Pathogen resistance Diseases 0.000 description 2
- 108010064851 Plant Proteins Proteins 0.000 description 2
- 241000710078 Potyvirus Species 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 241000710119 Sobemovirus Species 0.000 description 2
- 241000723790 Tobacco vein mottling virus Species 0.000 description 2
- 241000723848 Tobamovirus Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 244000098338 Triticum aestivum Species 0.000 description 2
- 108010067390 Viral Proteins Proteins 0.000 description 2
- 108050006628 Viral movement proteins Proteins 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000009418 agronomic effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000001506 fluorescence spectroscopy Methods 0.000 description 2
- 108020001507 fusion proteins Proteins 0.000 description 2
- 102000037865 fusion proteins Human genes 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 208000006278 hypochromic anemia Diseases 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 231100000518 lethal Toxicity 0.000 description 2
- 230000001665 lethal effect Effects 0.000 description 2
- 230000001338 necrotic effect Effects 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 102000013415 peroxidase activity proteins Human genes 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 230000002688 persistence Effects 0.000 description 2
- 235000021118 plant-derived protein Nutrition 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 238000002708 random mutagenesis Methods 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 231100000161 signs of toxicity Toxicity 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 230000029812 viral genome replication Effects 0.000 description 2
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical class O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000724330 Alfamovirus Species 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 102400000068 Angiostatin Human genes 0.000 description 1
- 108010079709 Angiostatins Proteins 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 101710096866 Calmodulin-related protein Proteins 0.000 description 1
- 241000282461 Canis lupus Species 0.000 description 1
- 241000252254 Catostomidae Species 0.000 description 1
- 241000701489 Cauliflower mosaic virus Species 0.000 description 1
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 description 1
- 240000002754 Chenopodium giganteum Species 0.000 description 1
- 235000008091 Chenopodium giganteum Nutrition 0.000 description 1
- 239000005496 Chlorsulfuron Substances 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000724253 Cucumovirus Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 239000005562 Glyphosate Substances 0.000 description 1
- HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 101000721661 Homo sapiens Cellular tumor antigen p53 Proteins 0.000 description 1
- 241000405425 Hura Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 108010092277 Leptin Proteins 0.000 description 1
- 102000016267 Leptin Human genes 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 240000007695 Nandina domestica Species 0.000 description 1
- 108020004485 Nonsense Codon Proteins 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000222291 Passalora fulva Species 0.000 description 1
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 1
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 206010035148 Plague Diseases 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 108010076039 Polyproteins Proteins 0.000 description 1
- 244000028344 Primula vulgaris Species 0.000 description 1
- 235000016311 Primula vulgaris Nutrition 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 101150023114 RNA1 gene Proteins 0.000 description 1
- 108050002653 Retinoblastoma protein Proteins 0.000 description 1
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
- 101150101169 Rx gene Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000242583 Scyphozoa Species 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 108700025695 Suppressor Genes Proteins 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 101710192266 Tegument protein VP22 Proteins 0.000 description 1
- 241000723717 Tobravirus Species 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 241000703392 Tribec virus Species 0.000 description 1
- 241000700618 Vaccinia virus Species 0.000 description 1
- 206010046865 Vaccinia virus infection Diseases 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- JUGOREOARAHOCO-UHFFFAOYSA-M acetylcholine chloride Chemical compound [Cl-].CC(=O)OCC[N+](C)(C)C JUGOREOARAHOCO-UHFFFAOYSA-M 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- FZCSTZYAHCUGEM-UHFFFAOYSA-N aspergillomarasmine B Natural products OC(=O)CNC(C(O)=O)CNC(C(O)=O)CC(O)=O FZCSTZYAHCUGEM-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 108010025267 calcium-dependent protein kinase Proteins 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000013256 coordination polymer Substances 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 108700010905 cucumovirus proteins Proteins 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000408 embryogenic effect Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
- 229940097068 glyphosate Drugs 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 230000009215 host defense mechanism Effects 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000035990 intercellular signaling Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 230000014634 leaf senescence Effects 0.000 description 1
- 229940039781 leptin Drugs 0.000 description 1
- NRYBAZVQPHGZNS-ZSOCWYAHSA-N leptin Chemical compound O=C([C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CC(C)C)CCSC)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CS)C(O)=O NRYBAZVQPHGZNS-ZSOCWYAHSA-N 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 235000019645 odor Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 235000009048 phenolic acids Nutrition 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 210000003449 plasmodesmata Anatomy 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 230000008593 response to virus Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000001743 silencing effect Effects 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 230000005100 tissue tropism Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 230000007919 viral pathogenicity Effects 0.000 description 1
- 230000006656 viral protein synthesis Effects 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9927609.9A GB9927609D0 (en) | 1999-11-22 | 1999-11-22 | Enhanced expression |
| GB0022960A GB0022960D0 (en) | 2000-09-19 | 2000-09-19 | Enhanced expression |
| GB0023668A GB0023668D0 (en) | 2000-09-27 | 2000-09-27 | Enhanced expression |
| PCT/GB2000/004454 WO2001038512A2 (en) | 1999-11-22 | 2000-11-22 | Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MXPA02005089A true MXPA02005089A (es) | 2003-01-28 |
Family
ID=27255894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MXPA02005089A MXPA02005089A (es) | 1999-11-22 | 2000-11-22 | Expresion de transgen mejorada por la coexpresion con un supresor del silenciamiento del gen post-transcripcional (ptgs). |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US7217854B1 (enExample) |
| EP (1) | EP1232274B1 (enExample) |
| JP (1) | JP4884628B2 (enExample) |
| KR (1) | KR20030004305A (enExample) |
| AT (1) | ATE460490T1 (enExample) |
| AU (1) | AU782788B2 (enExample) |
| CA (1) | CA2390152C (enExample) |
| DE (1) | DE60043992D1 (enExample) |
| IL (1) | IL149388A0 (enExample) |
| MX (1) | MXPA02005089A (enExample) |
| NZ (1) | NZ518947A (enExample) |
| WO (1) | WO2001038512A2 (enExample) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6972349B1 (en) | 1999-11-12 | 2005-12-06 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| CA2390049C (en) * | 1999-11-12 | 2012-01-24 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| GB0101505D0 (en) * | 2001-01-19 | 2001-03-07 | Scottish Crop Res Inst | Protection against gene silencing |
| GB0101513D0 (en) * | 2001-01-19 | 2001-03-07 | Scottish Crop Res Inst | 'Gene silencing suppressor' |
| CA2492917C (en) | 2002-07-19 | 2011-10-18 | University Of South Carolina | Compositions and methods for the modulation of gene expression in plants |
| US7683238B2 (en) | 2002-11-12 | 2010-03-23 | iBio, Inc. and Fraunhofer USA, Inc. | Production of pharmaceutically active proteins in sprouted seedlings |
| US7692063B2 (en) | 2002-11-12 | 2010-04-06 | Ibio, Inc. | Production of foreign nucleic acids and polypeptides in sprout systems |
| EP1594956A4 (en) | 2003-02-03 | 2007-08-01 | Fraunhofer Usa Inc | SYSTEM FOR EXPRESSION OF GENES IN PLANTS |
| AU2004278624B2 (en) * | 2003-10-01 | 2008-03-20 | Japan Science And Technology Agency | DNA fragment, method for producing transformant for protein production and utilization thereof |
| GB0327919D0 (en) * | 2003-12-02 | 2004-01-07 | Plant Bioscience Ltd | Enhanced expression |
| ES2532609T3 (es) * | 2004-02-20 | 2015-03-30 | Ibio, Inc. | Sistemas y métodos para expresión clonales en plantas |
| PL1756280T3 (pl) | 2004-04-22 | 2015-04-30 | Commw Scient Ind Res Org | Synteza długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach rekombinowanych |
| ES2529572T3 (es) | 2004-04-22 | 2015-02-23 | Commonwealth Scientific And Industrial Research Organisation | Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes |
| EP1616959A1 (en) * | 2004-07-07 | 2006-01-18 | Icon Genetics AG | Biological safe transient protein expression in plants |
| FR2877014B1 (fr) * | 2004-10-21 | 2009-07-10 | Inst Rech Pour Le Dev I R D Et | Systemes d'expression de proteines recombinantes et leurs applications |
| FR2880357B1 (fr) * | 2005-01-05 | 2010-12-10 | Inst Rech Developpement Ird | Vecteurs viraux pour la surexpression ou pour l'extinction de genes d'interet chez les plantes et leurs applications |
| WO2007092460A2 (en) * | 2006-02-03 | 2007-08-16 | The Texas A & M University System | Enhancing expression of value-added genes by transgenic expression of tombusvirus-based p19 gene mutants |
| BRPI0716075A2 (pt) | 2006-08-29 | 2013-08-06 | Commw Scient Ind Res Org | sÍntese de Ácidos graxos |
| EP1897953A1 (en) * | 2006-09-06 | 2008-03-12 | Icon Genetics GmbH | Potexvirus-derived replicon |
| US9605270B2 (en) * | 2006-09-22 | 2017-03-28 | The Regents Of The University Of California | Chemically inducible cucumber mosaic virus protein expression system |
| WO2008094512A2 (en) | 2007-01-29 | 2008-08-07 | The Ohio Sate University Research Foundation | System for expression of genes in plants from a virus-based expression vector |
| MX2009011893A (es) * | 2007-05-09 | 2010-02-17 | Devgen Nv | Metodo para la transcripcion sustentable de transgenes. |
| US8513397B2 (en) * | 2008-08-27 | 2013-08-20 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | DNA replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants |
| CA3015426C (en) | 2008-11-18 | 2020-01-14 | Commonwealth Scientific And Industrial Research Organisation | Recombinant cells comprising exogenous .delta.5 and .delta.6 desaturases, .delta.5 and .delta.6 elongases, and .delta.4 desaturases |
| AU2013204254B8 (en) * | 2008-11-18 | 2015-10-08 | Commonwealth Scientific And Industrial Research Organisation | Enzymes and methods for producing omega-3 fatty acids |
| WO2010099462A2 (en) * | 2009-02-26 | 2010-09-02 | Baylor University | Highly efficient suppressor-dependent protein expression in plants with a viral vector |
| US20110197312A1 (en) * | 2010-02-07 | 2011-08-11 | University Of Kuwait | Indigenous Viral Satellite RNA Associate with Cucumber Mosaic Virus used as a Biological Control Agent against Tomato Diseases caused by Virus Strains, Viroids, and Fungi |
| FR2960550A1 (fr) * | 2010-05-26 | 2011-12-02 | Inst Rech R Pour Le Dev Ird | Moyens pour la production transitoire dans les plantes de proteines recombinantes utilisables notamment en prophylaxie et en therapeutique |
| FI20100248A0 (fi) | 2010-06-11 | 2010-06-11 | Kristiina Maekinen | Menetelmä proteiinien tuottamiseksi |
| CA2824152C (en) | 2011-01-17 | 2022-12-13 | Philip Morris Products S.A. | Method and system for producing a heterologous polypeptide in a nicotiana tabacum plant |
| WO2012098111A1 (en) | 2011-01-17 | 2012-07-26 | Philip Morris Products S.A. | Vectors for nucleic acid expression in plants |
| KR101334846B1 (ko) * | 2011-07-20 | 2013-11-29 | 대한민국 | 벼줄무늬잎마름병 바이러스 유래 ｎｓ3유전자의 신규 용도 |
| WO2013096992A1 (en) * | 2011-12-27 | 2013-07-04 | Commonwealth Scientific And Industrial Reserach Organisation | Simultaneous gene silencing and supressing gene silencing in the same cell |
| HUE033766T2 (en) | 2012-06-15 | 2018-01-29 | Commw Scient Ind Res Org | Production of long chain polyunsaturated fatty acids in plant cells |
| EA037817B1 (ru) | 2013-12-18 | 2021-05-25 | Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн | Экстрагированный растительный липид, содержащий длинноцепочечные полиненасыщенные жирные кислоты |
| WO2015168733A1 (en) * | 2014-05-05 | 2015-11-12 | Queensland University Of Technology | Decoy molecules |
| CA2953008C (en) | 2014-06-27 | 2024-03-19 | Nuseed Pty Ltd | Lipid comprising docosapentaenoic acid |
| WO2019049111A1 (en) | 2017-09-11 | 2019-03-14 | R. J. Reynolds Tobacco Company | METHODS AND COMPOSITIONS FOR INCREASING THE EXPRESSION OF GENES OF INTEREST IN A PLANT BY CO-EXPRESSION WITH P21 |
| US20220002746A1 (en) * | 2018-11-13 | 2022-01-06 | University Of Maryland, College Park | Plant Vectors, Compositions and Uses Relating Thereto |
| CN115851820B (zh) * | 2022-12-19 | 2024-06-18 | 内蒙古农业大学 | 一株烟草脉斑驳病毒的应用 |
| CN119410670A (zh) * | 2025-01-06 | 2025-02-11 | 中国热带农业科学院三亚研究院 | 斯里兰卡木薯花叶病毒转录激活因子ac2及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5939541A (en) * | 1997-03-28 | 1999-08-17 | University Of South Carolina | Method for enhancing expression of a foreign or endogenous gene product in plants |
| US6972349B1 (en) * | 1999-11-12 | 2005-12-06 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| CA2390049C (en) * | 1999-11-12 | 2012-01-24 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
-
2000
- 2000-11-22 WO PCT/GB2000/004454 patent/WO2001038512A2/en not_active Ceased
- 2000-11-22 AU AU15334/01A patent/AU782788B2/en not_active Ceased
- 2000-11-22 JP JP2001540262A patent/JP4884628B2/ja not_active Expired - Fee Related
- 2000-11-22 MX MXPA02005089A patent/MXPA02005089A/es unknown
- 2000-11-22 CA CA2390152A patent/CA2390152C/en not_active Expired - Lifetime
- 2000-11-22 US US10/130,758 patent/US7217854B1/en not_active Expired - Lifetime
- 2000-11-22 KR KR1020027006548A patent/KR20030004305A/ko not_active Ceased
- 2000-11-22 EP EP00977696A patent/EP1232274B1/en not_active Expired - Lifetime
- 2000-11-22 AT AT00977696T patent/ATE460490T1/de not_active IP Right Cessation
- 2000-11-22 DE DE60043992T patent/DE60043992D1/de not_active Expired - Lifetime
- 2000-11-22 IL IL14938800A patent/IL149388A0/xx not_active IP Right Cessation
- 2000-11-22 NZ NZ518947A patent/NZ518947A/en unknown
-
2006
- 2006-11-20 US US11/602,416 patent/US8871997B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US8871997B2 (en) | 2014-10-28 |
| US7217854B1 (en) | 2007-05-15 |
| KR20030004305A (ko) | 2003-01-14 |
| JP2003514585A (ja) | 2003-04-22 |
| ATE460490T1 (de) | 2010-03-15 |
| AU782788B2 (en) | 2005-08-25 |
| IL149388A0 (en) | 2002-11-10 |
| JP4884628B2 (ja) | 2012-02-29 |
| WO2001038512A3 (en) | 2002-03-28 |
| AU1533401A (en) | 2001-06-04 |
| CA2390152A1 (en) | 2001-05-31 |
| EP1232274A2 (en) | 2002-08-21 |
| CA2390152C (en) | 2012-08-28 |
| EP1232274B1 (en) | 2010-03-10 |
| DE60043992D1 (de) | 2010-04-22 |
| WO2001038512A2 (en) | 2001-05-31 |
| NZ518947A (en) | 2004-05-28 |
| US20070292862A1 (en) | 2007-12-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1232274B1 (en) | Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs) | |
| US6531647B1 (en) | Gene silencing methods | |
| Rajamaki et al. | Control of nuclear and nucleolar localization of nuclear inclusion protein a of picorna-like Potato virus A in Nicotiana species | |
| Ratcliff et al. | Technical advance: tobacco rattle virus as a vector for analysis of gene function by silencing | |
| Dunoyer et al. | Retracted: Identification, subcellular localization and some properties of a cysteine‐rich suppressor of gene silencing encoded by peanut clump virus | |
| Mangwende et al. | The P0 gene of Sugarcane yellow leaf virus encodes an RNA silencing suppressor with unique activities | |
| Saxena et al. | Improved foreign gene expression in plants using a virus‐encoded suppressor of RNA silencing modified to be developmentally harmless | |
| US7229829B2 (en) | Tobacco rattle virus vectors and related compositions and methods | |
| Sempere et al. | Development of expression vectors based on pepino mosaic virus | |
| Gao et al. | Enhanced transgene expression in sugarcane by co-expression of virus-encoded RNA silencing suppressors | |
| Ehrenfeld et al. | Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants | |
| CN101092634B (zh) | 一种培育抗黄瓜花叶病毒植物的方法 | |
| Rubino et al. | Biologically active cymbidium ringspot virus satellite RNA in transgenic plants suppresses accumulation of DI RNA | |
| US20050214263A1 (en) | Methods and means for gene silencing in plants | |
| CA2297616A1 (en) | Viral vectors | |
| US20110154538A1 (en) | Infectious Plant Viral Vector and An Artificial Bipartite Plant Viral Vector An Infectious Plant Viral Vector and An Artificial Bipartite Plant Viral Vector | |
| WO2011154611A2 (en) | A method for enhanced protein synthesis | |
| Doreste et al. | Transgenic potato plants expressing the potato virus X (PVX) coat protein gene developed resistance to the viral infection | |
| WO2008119136A1 (en) | Improved methods and constructs for marker free agrobacterium mediated transformatiom | |
| Han et al. | Gene silencing-mediated resistance in transgenic tobacco plants carrying potato virus Y coat protein gene | |
| Vasudevan et al. | Characterization of resistance mechanism in transgenic Nicotiana benthamiana containing Turnip crinkle virus coat protein | |
| Sarkar | Suppressor activity analysis of Tomato leaf curl New Delhi virus gene (s) and development of RNAi construct to evaluate their efficacy in inhibiting the virus. | |
| Jin et al. | Expression of the tobacco mosaic virus movement protein alters starch accumulation in Nicotiana benthamiana | |
| Doreste et al. | Transgenic Potato Plants Expressing the Coat Protein Gene Potato Virus X (PVX) Developed Resistance to the Viral Infection | |
| Gao et al. | Enhanced Transgene Expression in Sugarcane by Co-Expression of Virus |