AU782788B2 - Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (PTGS) - Google Patents

Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (PTGS) Download PDF

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Publication number
AU782788B2
AU782788B2 AU15334/01A AU1533401A AU782788B2 AU 782788 B2 AU782788 B2 AU 782788B2 AU 15334/01 A AU15334/01 A AU 15334/01A AU 1533401 A AU1533401 A AU 1533401A AU 782788 B2 AU782788 B2 AU 782788B2
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AU
Australia
Prior art keywords
gfp
pvx
ptgs
protein
plant
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU15334/01A
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English (en)
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AU1533401A (en
Inventor
David Charles Baulcombe
Andrew John Hamilton
Olivier Voinnet
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Plant Bioscience Ltd
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Plant Bioscience Ltd
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Filing date
Publication date
Priority claimed from GBGB9927609.9A external-priority patent/GB9927609D0/en
Priority claimed from GB0022960A external-priority patent/GB0022960D0/en
Priority claimed from GB0023668A external-priority patent/GB0023668D0/en
Application filed by Plant Bioscience Ltd filed Critical Plant Bioscience Ltd
Publication of AU1533401A publication Critical patent/AU1533401A/en
Application granted granted Critical
Publication of AU782788B2 publication Critical patent/AU782788B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203Virus mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AU15334/01A 1999-11-22 2000-11-22 Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (PTGS) Ceased AU782788B2 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GBGB9927609.9A GB9927609D0 (en) 1999-11-22 1999-11-22 Enhanced expression
GB9927609 1999-11-22
GB0022960A GB0022960D0 (en) 2000-09-19 2000-09-19 Enhanced expression
GB0022960 2000-09-19
GB0023668A GB0023668D0 (en) 2000-09-27 2000-09-27 Enhanced expression
GB0023668 2000-09-27
PCT/GB2000/004454 WO2001038512A2 (en) 1999-11-22 2000-11-22 Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs)

Publications (2)

Publication Number Publication Date
AU1533401A AU1533401A (en) 2001-06-04
AU782788B2 true AU782788B2 (en) 2005-08-25

Family

ID=27255894

Family Applications (1)

Application Number Title Priority Date Filing Date
AU15334/01A Ceased AU782788B2 (en) 1999-11-22 2000-11-22 Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (PTGS)

Country Status (12)

Country Link
US (2) US7217854B1 (enExample)
EP (1) EP1232274B1 (enExample)
JP (1) JP4884628B2 (enExample)
KR (1) KR20030004305A (enExample)
AT (1) ATE460490T1 (enExample)
AU (1) AU782788B2 (enExample)
CA (1) CA2390152C (enExample)
DE (1) DE60043992D1 (enExample)
IL (1) IL149388A0 (enExample)
MX (1) MXPA02005089A (enExample)
NZ (1) NZ518947A (enExample)
WO (1) WO2001038512A2 (enExample)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60029986T2 (de) * 1999-11-12 2007-09-06 University Of South Carolina Kontrolle der post-transkriptionellen Genabschaltung (gene silencing) in Pflanzen
US6972349B1 (en) 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
GB0101513D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst 'Gene silencing suppressor'
GB0101505D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst Protection against gene silencing
US20040268441A1 (en) 2002-07-19 2004-12-30 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
US7683238B2 (en) 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
US7692063B2 (en) 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
US8148608B2 (en) * 2004-02-20 2012-04-03 Fraunhofer Usa, Inc Systems and methods for clonal expression in plants
EP1594956A4 (en) 2003-02-03 2007-08-01 Fraunhofer Usa Inc SYSTEM FOR EXPRESSION OF GENES IN PLANTS
CA2540668C (en) * 2003-10-01 2010-12-21 Japan Science And Technology Agency Dna fragment, method for producting transformant for producing transformant for protein production and utilization thereof
GB0327919D0 (en) * 2003-12-02 2004-01-07 Plant Bioscience Ltd Enhanced expression
CN102559364B (zh) 2004-04-22 2016-08-17 联邦科学技术研究组织 用重组细胞合成长链多不饱和脂肪酸
CA2563875C (en) 2004-04-22 2015-06-30 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
EP1616959A1 (en) * 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants
FR2877014B1 (fr) * 2004-10-21 2009-07-10 Inst Rech Pour Le Dev I R D Et Systemes d'expression de proteines recombinantes et leurs applications
FR2880357B1 (fr) * 2005-01-05 2010-12-10 Inst Rech Developpement Ird Vecteurs viraux pour la surexpression ou pour l'extinction de genes d'interet chez les plantes et leurs applications
US8222488B2 (en) 2006-02-03 2012-07-17 Texas A&M University System Enhancing expression of value-added genes by transgenic expression of tombusvirus-based P19 gene mutants
BRPI0716075A2 (pt) 2006-08-29 2013-08-06 Commw Scient Ind Res Org sÍntese de Ácidos graxos
EP1897953A1 (en) * 2006-09-06 2008-03-12 Icon Genetics GmbH Potexvirus-derived replicon
WO2008036424A2 (en) * 2006-09-22 2008-03-27 Regents Of The University Of California Chemically inducible cucumber mosaic virus protein expression system
WO2008094512A2 (en) 2007-01-29 2008-08-07 The Ohio Sate University Research Foundation System for expression of genes in plants from a virus-based expression vector
EP2164969A1 (en) * 2007-05-09 2010-03-24 Devgen NV A method for sustainable transgene transcription
WO2010025285A1 (en) * 2008-08-27 2010-03-04 Arizona Board Of Regents For And On Behalf Of Arizona State University A dna replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants
CN114045301A (zh) 2008-11-18 2022-02-15 联邦科学技术研究组织 产生ω-3脂肪酸的酶和方法
AU2013204270B8 (en) * 2008-11-18 2015-06-04 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
WO2010099462A2 (en) * 2009-02-26 2010-09-02 Baylor University Highly efficient suppressor-dependent protein expression in plants with a viral vector
US20110197312A1 (en) * 2010-02-07 2011-08-11 University Of Kuwait Indigenous Viral Satellite RNA Associate with Cucumber Mosaic Virus used as a Biological Control Agent against Tomato Diseases caused by Virus Strains, Viroids, and Fungi
FR2960550A1 (fr) 2010-05-26 2011-12-02 Inst Rech R Pour Le Dev Ird Moyens pour la production transitoire dans les plantes de proteines recombinantes utilisables notamment en prophylaxie et en therapeutique
FI20100248A0 (fi) 2010-06-11 2010-06-11 Kristiina Maekinen Menetelmä proteiinien tuottamiseksi
EP2665818B1 (en) 2011-01-17 2017-03-22 Philip Morris Products S.a.s. Protein expression in plants
WO2012098111A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
KR101334846B1 (ko) * 2011-07-20 2013-11-29 대한민국 벼줄무늬잎마름병 바이러스 유래 ns3유전자의 신규 용도
DK2798065T3 (da) * 2011-12-27 2019-09-16 Commw Scient Ind Res Org Samtidig geninaktivering og undertrykkelse af geninaktivering i samme celle
PT2861059T (pt) 2012-06-15 2017-08-08 Grains Res & Dev Corp Produção de ácidos gordos polinsaturados de cadeia longa em células vegetais
WO2015089587A1 (en) 2013-12-18 2015-06-25 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
WO2015168733A1 (en) * 2014-05-05 2015-11-12 Queensland University Of Technology Decoy molecules
PH12016502586B1 (en) 2014-06-27 2023-07-19 Commw Scient Ind Res Org Lipid comprising docosapentaenoic acid
US11649465B2 (en) 2017-09-11 2023-05-16 R.J. Reynolds Tobacco Company Methods and compositions for increasing expression of genes of interest in a plant by co-expression with p21
CN113302308A (zh) * 2018-11-13 2021-08-24 马里兰大学派克分院 植物载体、组合物和与其相关的用途
CN115851820B (zh) * 2022-12-19 2024-06-18 内蒙古农业大学 一株烟草脉斑驳病毒的应用
CN119410670A (zh) * 2025-01-06 2025-02-11 中国热带农业科学院三亚研究院 斯里兰卡木薯花叶病毒转录激活因子ac2及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998044097A2 (en) * 1997-03-28 1998-10-08 Vance Vicki B Method for enhancing expression of a foreign or endogenous gene product in plants

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6972349B1 (en) * 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
DE60029986T2 (de) * 1999-11-12 2007-09-06 University Of South Carolina Kontrolle der post-transkriptionellen Genabschaltung (gene silencing) in Pflanzen

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998044097A2 (en) * 1997-03-28 1998-10-08 Vance Vicki B Method for enhancing expression of a foreign or endogenous gene product in plants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRIGNETI, G. ET AL., 1998, EMBO JOURNAL, 17: 6739-6746 *

Also Published As

Publication number Publication date
IL149388A0 (en) 2002-11-10
ATE460490T1 (de) 2010-03-15
JP4884628B2 (ja) 2012-02-29
US20070292862A1 (en) 2007-12-20
CA2390152C (en) 2012-08-28
US8871997B2 (en) 2014-10-28
JP2003514585A (ja) 2003-04-22
KR20030004305A (ko) 2003-01-14
US7217854B1 (en) 2007-05-15
NZ518947A (en) 2004-05-28
WO2001038512A3 (en) 2002-03-28
CA2390152A1 (en) 2001-05-31
DE60043992D1 (de) 2010-04-22
WO2001038512A2 (en) 2001-05-31
EP1232274B1 (en) 2010-03-10
AU1533401A (en) 2001-06-04
EP1232274A2 (en) 2002-08-21
MXPA02005089A (es) 2003-01-28

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